[ Objective ] The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [ Method ] Ten kinds of com- mon commercial bactericides preliminarily screened were selected...[ Objective ] The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [ Method ] Ten kinds of com- mon commercial bactericides preliminarily screened were selected for indoor antibacterial test using paper plate method and plate streaking method, and six kinds of bactericides with good antibacterial effects were further screened for indoor toxicity test. [ Result] Chloramphenicoloethylicin had the best antibacterial effect, with EC50 of 36.34 rag/L; followed by Xintianxlucai No. 6 and embamycin, with EC50 of 83.21 and 92.34 mg/L, respectively. [ Conclusion] Cldoramphenicoloethylicin, Xintianxiucai No. 6 and embamycin screened in the test had good antibacterial effect against kiwifruit bacteriai canker in Wanzhou District, and chlorampheni- coleethylicin was the most effective agent. These three agents could be directly applied to control kiwifruit bacterial canker in production, while application time and climate conditions in fields should also be considered during the actual application process. It is suggested that three agents can be used alternatively in order to de- lay the resistance of plants.展开更多
The toxicity of 12 kinds of bactericides against the pathogen of cassava bacterial blight （Xanthomonas axonopodis pv. manihotis） was determined by tox- ic medium method. The results showed that 2% triadimefon ·...The toxicity of 12 kinds of bactericides against the pathogen of cassava bacterial blight （Xanthomonas axonopodis pv. manihotis） was determined by tox- ic medium method. The results showed that 2% triadimefon · 30% ethylicin EC, 80% ethylicin EC, 70% mancozeb WP and 72% strepolin SP had the lowest ef- fective concentration and the best inhibitory effect, while 12.5% myclobutanil SP, 20% thiodiazole-copper SC and 20% bismerthlazol WP had the highest effective concentration but the worst inhibitory effect. Field test of six kinds of bactericides was conducted in Zhanjiang City of Guangdong Province in 2011, and the results showed that 3% zhongshengrnycin WP, 2% triadimefon · 30% ethylicin EC and 80% ethylicin EC had good control effect, which were suggested to be used for field control.展开更多
Protease-assisted soaking has received increasing attention in recent years.However,few reports have elaborated on the effect of bactericides,which are used to protect raw hides from microbial damage in the soaking pr...Protease-assisted soaking has received increasing attention in recent years.However,few reports have elaborated on the effect of bactericides,which are used to protect raw hides from microbial damage in the soaking process,on the performance of protease-assisted soaking.Here we investigate the effects of three bactericides,namely,2-methyl-4-isothiazolin-3-one(MIT),sodium propyl 4-hydroxybenzoate(SPHB)and cetyl trimethyl ammonium bromide(CTAB),on the catalytic activity of protease.MIT and SPHB have little effect on the proteolytic activity,whilst CTAB has a negative effect.Fluorescence spectroscopy,synchronous fluorescence spectroscopy,molecular docking and molecular dynamics simulation were used to analyse the bactericide-protease interaction.The data reveal that MIT and SPHB are bound to the non-catalytic sites of protease,whilst CTAB affects the catalytic triad of protease.Furthermore,the protease and bactericides were used alone,simultaneously and sequentially in the soaking process,and their soaking performances were evaluated.The evaluation shows that the use of protease increases the microorganisms in the soaking float,and MIT exhibits the best bactericidal effect.The simultaneous use of protease and MIT effectively inhibits bacteria and scarcely affects the removal of unstructured proteins from hides and the attack on epidermis by protease.These findings contribute to a better understanding of the scientific use of protease with other auxiliaries in soaking.展开更多
Jasmine essential oil and lemon essential oil were selected for deodorizing ingredient.Lysine and silver ions were selected as auxiliary raw material.The product was prepared by emulsion dissolution technology.The min...Jasmine essential oil and lemon essential oil were selected for deodorizing ingredient.Lysine and silver ions were selected as auxiliary raw material.The product was prepared by emulsion dissolution technology.The minimum inhibitory concentration of various bacteria were during 100~2,000 mg/L.In the test,the germicidal efficiency of 2.0%(w/w)deodorant was 99%.The removal efficiency of trimethylamine and methanthiol were more than 95%by 3.0%(w/w).deodorant.The removal efficiency of ammonia and methanthiol were more than 92%by 3.0%(w/w)deodorant.In addition,The removal efficiency of isovaleric acid was 98%by 2.0%(w/w)deodorant.In analysis result,common pathogenic bacteria were effectively suppressed,kitchen and toilet odors were efficiently removed by natural plant deodorant.展开更多
In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,name...In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.展开更多
Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for c...Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for catheter coating materials.Photothermal therapy(PTT)is a highly efficient bactericidal method.Here,a composite hydrogel containing MXene nanosheets and hydrophilic 3-sulfopropyl methacrylate potassium salt(SPMK)is reported,which is synthesized through the one-pot method and heat-initiated polymerization.The hydrogel shows excellent antibacterial performance against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)in 3 min in the air or 20 min in the water environment under near-infrared light(NIR;808 nm)irradiation.The friction coefficient of the hydrogel is about 0.11,which is 48%lower than that without SPMK.The rapid photothermal sterilization is attributed to the outstanding antibacterial ability and thermal effect of photoactivated MXene.The ultra-low friction is the result of the hydration lubrication mechanism.This study provides a potential strategy for the surface coatings of biomedical catheters,which enables rapid sterilization and extremely low interface resistance between catheters and biological tissues.展开更多
Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive ...Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive species,which can be regulated by the activation process.In this study,surface plasma-activated air(SAA)and a He+O_(2)plasma jet(Jet)were parallelly combined(the SAA+Jet combination)or sequentially combined(the SAA→Jet combination and the Jet→SAA combination)to prepare plasma-activated saline(PAS).The PAS activated by the combinations exhibited stronger bactericidal effects than that activated by the SAA or the Jet alone.The concentrations of H_(2)O_(2)and NO_(2)^(-)were higher in the PAS activated by the Jet→SAA combination,while ONOO^(-)concentrations were close in the three kinds of PAS and^(1)O_(2)concentrations were higher in the PAS activated by the SAA+Jet combination.The analysis of scavengers also demonstrated that H_(2)O_(2),^(1)O_(2),and ONOO^(-)in the PAS activated by the SAA+Jet combination,and^(1)O_(2)in the PAS activated by the Jet→SAA combination played critical roles in bactericidal effects.Further,the effective placement time of the three PAS varied,and the PAS activated by the Jet→SAA combination could also inactivate 2.6-log_(10)of MRSA cells after placement for more than 60 min.The regulation of reactive species in plasma-activated water via different combinations of plasma devices could improve the directional application of plasma-activated water in the biomedical field.展开更多
Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated ...Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.展开更多
Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 202...Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 2022. Methodology: During this eleven-month prospective study, we set up a quality control device (QCD). For microbiological quality control, we performed different cultures of bacteria with different bacteriological and morphological characteristics at T0 (no sterilization) and at T30 (after 30 minutes of sterilization under DISTER-UV). Results: After the realization, the DCQ attached to the DISTER-UV1 allows to display of the UV wavelength present in the light box. This device also displays and alerts when the UV intensity emitted by the lamps is below 250 nm. During microbiological quality control, the cultures carried out at T30 and incubated for 24 hours did not reveal any bacterial colonies. This shows the bactericidal character of DISTER-UV-2. Conclusion: The improvement and the microbiological quality control allowed us to switch from DISTER-UV1 (without sensor) to DISTER-UV-2 (with sensor or DCQ). The biological control allowed us to affirm that the DISTER-UV-2 is bactericidal.展开更多
This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family.The inhibitory rate,minimum inhibitory concentration(MIC),and minimum ba...This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family.The inhibitory rate,minimum inhibitory concentration(MIC),and minimum bactericidal concentration(MBC)of leaf and stem,and root and rhizome extracts from Alpinia katsumadai Hayata,Alpinia oxyphylla Miq×Alpinia henryi K.Schumann,Alpinia oblongifolia Hayata,Alpinia nigra(Gaertn.)Burtt,Amomum villosum Lour,Alpinia zerumbet(Pers.)Burtt.et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method.The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi.At a 1.5 mg mL^(−1),A.zerumbet root and rhizome extracts exhibited strong inhibitory activity against S.aureus and E.coli,with 83.23%and 79.62%,respectively.In addition,A.zerumbet leaf and stem extracts had an inhibitory rate of 90.85%against P.aeruginosa.At the same concentration,the leaf and stem,root and rhizome extracts of A.katsumadai had the best anti-bacterial effect against F.oxysporum,with inhibition rates of 84.46%and 84.73%,respectively.Moreover,A.katsumadai and A.zerumbet leaf and stem extracts had the most significant antibacterial effect against S.aureus,with a MIC of 0.063 mg mL^(−1).Thus,both A.katsumadai and A.zerumbet extracts had significant antibacterial activity.In addition,by comparing the inhibitory effect of extracts from different parts,it was found that the inhibitory rate and average inhibitory rate of extracts from leaf and stem were higher than those from root and rhizome.The chemical constituents of A.katsumadai and A.zerumbet,determined by the high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),revealed that citric acid(CA),alpinetin,and pinocembrin(PNCB)were the functional constituents yielding the antibacterial activity.Overall,A.katsumadai and A.zerumbet have the potential to be developed as new plant fungicides and bactericides.展开更多
The determination of 7 bactericidals residues,including chlorthalonil,dichlofluanid,tolylfluanid,captan,folpet,captafol and deltamethrin,in foods by using on-line gel permeation chromatography(GPC) coupled with gas ch...The determination of 7 bactericidals residues,including chlorthalonil,dichlofluanid,tolylfluanid,captan,folpet,captafol and deltamethrin,in foods by using on-line gel permeation chromatography(GPC) coupled with gas chromatography-mass spectrometry(GC-MS).Bactericidal residues were extracted with acetonitrile,and cleaned up by ENVI-Crab,LC-NH2 column and GPC,respectively.The analysis of 7 bactericidals residues was performed by GC-MS.The average recoveries of analytes were range of 75%-120% with RSD of 3.5%-10.3%.展开更多
The application of cutting fluid in the field of engineering manufacturing has a history of hundreds of years,and it plays a vital role in the processing efficiency and surface quality of parts.Among them,water-based ...The application of cutting fluid in the field of engineering manufacturing has a history of hundreds of years,and it plays a vital role in the processing efficiency and surface quality of parts.Among them,water-based cutting fluid accounts for more than 90%of the consumption of cutting fluid.However,long-term recycling of water-based cutting fluid could easily cause deterioration,and the breeding of bacteria could cause the cutting fluid to fail,increase manufacturing costs,and even endanger the health of workers.Traditional bactericides could improve the biological stability of cutting fluids,but they are toxic to the environment and do not conform to the development trend of low-carbon manufacturing.Low-carbon manufacturing is inevitable and the direction of sustainable manufacturing.The use of nanomaterials,transition metal complexes,and physical sterilization methods on the bacterial cell membrane and genetic material could effectively solve this problem.In this article,the mechanism of action of additives and microbial metabolites was first analyzed.Then,the denaturation mechanism of traditional bactericides on the target protein and the effect of sterilization efficiency were summarized.Further,the mechanism of nanomaterials disrupting cell membrane potential was discussed.The effects of lipophilicity and the atomic number of transition metal complexes on cell membrane penetration were also summarized,and the effects of ultraviolet rays and ozone on the destruction of bacterial genetic material were reviewed.In other words,the bactericidal performance,hazard,degradability,and economics of various sterilization methods were comprehensively evaluated,and the potential development direction of improving the biological stability of cutting fluid was proposed.展开更多
ByBimolecular Nucleophilic Substitution, four new types of alkylene triphenyl double quaternary phosphonium salt were synthesized respectively by using triphenylphosphine, 1,3-dibromopropane, 1,6-dibromohexane,1,10-di...ByBimolecular Nucleophilic Substitution, four new types of alkylene triphenyl double quaternary phosphonium salt were synthesized respectively by using triphenylphosphine, 1,3-dibromopropane, 1,6-dibromohexane,1,10-dibromo- decane, 1,12-dibromododecane as raw materials and using DMAC as the solvent, under a certain temperature and reac- tion time. The productivity is 58% - 83%. The molecular structures of the products were characterized by IR, NMR and elemental analysis. The sterilizing effect of 1,6-hexylidene triphenyl double phosphonium bromide(HTDPB) and 1,12- dodecylidene triphenyl double phosphonium bromide(DoTDPB) was evaluated by using extinct dilution method.The experimental result shows that the sterilizing effect of DoTDPB is better than the effect of HTDPB under the same drug concentration and contact time. When the concentration of DoTDPB was 20 mg/L and the contact time was 0.5 h, the sterilizing rate of DoTDPB used to kill saprophytic bacteria (TGB), sulfate-reducing bacteria (SRB) and iron bacteria (IB) was 95.56%, 84% and 99.58% respectively.展开更多
By way of bactericidal halo test, the antibacterial performance of various electroplated deposits (cobalt, zinc, copper, silver, gold, nickel, chromium, cobalt-containing alloy of nickel, and nickel-containing alloy o...By way of bactericidal halo test, the antibacterial performance of various electroplated deposits (cobalt, zinc, copper, silver, gold, nickel, chromium, cobalt-containing alloy of nickel, and nickel-containing alloy of cobalt) were determined. The results of the test show that the electroplated deposits of zinc, cobalt, cobalt-containing alloy of nickel, and nickel-containing alloy of cobalt, silver and copper possess a distinct antibacterial capability against the growth of pathogenic bacteria tested, and that SUS304 stainless steel and chromium deposit have no antibacterial activity. The relationship between the antibacterial capability and the rest potentials of the deposits was investigated. It was found that the higher the antibacterial activity of the electroplated deposit was, the more negative the rest potential of deposit was.展开更多
This work is a result of previously done studies on the synthesis of A2FeVIO4 wet ferrate (VI) formula, using chlorine as an oxidant. The major problem of these ferrates is related to their stability over time. This b...This work is a result of previously done studies on the synthesis of A2FeVIO4 wet ferrate (VI) formula, using chlorine as an oxidant. The major problem of these ferrates is related to their stability over time. This brings us to identify and optimize the critical parameters influencing the preparation of the Na2FeO4 at room stable phase with acceptable performance. The use of water bleach (hypochlorite ClO‾) at a chlorometric degree of 50°F in the synthesis of the Na2FeO4 ambient stable phase promotes the oxidation of iron (II) iron to (VI) in a concentrated NaOH alkaline medium. The synthesis reaction is in the presence of FeSO4 7H2O hydrated iron sulfate at a temperature of about 55°C in order to simplify the synthesis process, to enhance the production of the Fe (VI) and to meet the growing demand of ferrates (VI) for their interest in the treatment of water. Monitoring the degradation of synthesized Na2FeO4 shows its stability up to 12 months, which facilitates storage and transportation. The phases obtained were characterized by IR spectroscopy, and RX by UV spectrophotometer, measuring the optical density at 507 nm.展开更多
Quaternary ammonium compound are a class of broad-spectrum,high-efficiency and low-toxicity bactericide,which are widely used in daily life and industrial sterilization.Herein,the current state of domestic quaternary ...Quaternary ammonium compound are a class of broad-spectrum,high-efficiency and low-toxicity bactericide,which are widely used in daily life and industrial sterilization.Herein,the current state of domestic quaternary ammonium disinfectant products was summarized,as well as those new products developed and studied,and the development trend was also predicted.展开更多
The iron compounds in the oxidation state (VI) have the specific advantage of being powerful oxidants and bactericides. This feature explains their particular interest in the treatment of water. The aim of this work i...The iron compounds in the oxidation state (VI) have the specific advantage of being powerful oxidants and bactericides. This feature explains their particular interest in the treatment of water. The aim of this work is to prepare Na2FeO4 stable at ambient in order to optimize the key parameters influencing the performance of the oxidation of iron (II) to iron (VI), as well as to monitor its degradation over time. The synthesis of this phase has been carried out by using the dry reaction Na2O2 with Fe2O3 with a temperature of 700°C for a reaction time of 13 hours with a Na/Fe ratio of 4 to make it possible to simplify the synthesis procedure, to minimize the cost and enhance the production of iron (VI) to meet the growing demand of ferrate (VI) for its interest in water treatment. The obtained phase was characterized by UV spectrophotometer by measuring the optical density at a wavelength of 507 nm.展开更多
Essential oils (EOs) are natural bioactive compounds with antibacterial activity against a variety of microorganisms including phytopathogens. The use of EOs and their components as viable therapeutic antibacterials i...Essential oils (EOs) are natural bioactive compounds with antibacterial activity against a variety of microorganisms including phytopathogens. The use of EOs and their components as viable therapeutic antibacterials is however greatly compromised by their volatile nature, hydrophobicity and instability when exposed to environmental and physiological factors. Encapsulation of these compounds in an appropriate carrier system can alleviate these challenges. This study therefore aimed at developing, characterizing and evaluating the efficacy of antibacterial potential of thymol and eugenol loaded chitosan nanoparticles (TCNPs and ECNPs) against <i>Ralstonia solanacearum</i>, the bacterial wilt-causing pathogen in potatoes. Synthesis of TCNP and ECNP was achieved via ionic gelation method and the prepared nanoparticles characterized by their particle size distributions, encapsulation efficiency, loading capacity (LC) and <i>in-vitro</i> release characteristics. Antibacterial activities of the nanoparticles were investigated using agar dilution and colony counting methods and their minimum inhibitory concentration (MIC) determined by 96-well broth micro-dilution method. Scanning electron microscope images of TCNPs and ECNPs showed that the nanoparticles were spherical in shape and were well separated with an average particle size of 590 nm and 555 nm respectively. The average size of chitosan nanoparticles alone was however 375 nm. The encapsulation efficiency was 72.9% for TCNP (with 48.3% LC) and 71.7% for ECNP (with 49.5% LC). The release of thymol and eugenol from the microcapsules was found to be pH dependent with the highest release at pH 1.5. The growth inhibition of <i>R. solanacearum</i> was 92% and 94% for TCNP and ECNPs respectively. The MIC of thymol and eugenol before encapsulation was 175 μg/ml and 275 μg/ml respectively, but this reduced significantly to 22.5 μg/ml and 45 μg/ml after encapsulation. Thus, encapsulation of thymol and eugenol in chitosan nanoparticles has shown promising potential as a bactericide alternative for <i>R. solanacearum</i> and could be useful in managing the soil borne phytopathogen.展开更多
Aryloxy--terminated polydimethylsiloxanes (AOS) were synthesized by the telomerization of hexamethylcyclotrisiloxane(D3) with phenols. The structures of AOS were characterized by UV, IR, 2HNMR and elemental analysis, ...Aryloxy--terminated polydimethylsiloxanes (AOS) were synthesized by the telomerization of hexamethylcyclotrisiloxane(D3) with phenols. The structures of AOS were characterized by UV, IR, 2HNMR and elemental analysis, The effects of the reaction conditions and the mole ratios of the reactants on foe molecular weight of the polymer were discussed. A OS's structural influence on bactericidal action, flocculation and hydrolysis was studied.展开更多
Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on se...Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on sensory and physiochemical quality indices, detergency and foaming performance of laundry detergent were comprehensively investigated,and antimicrobial performance as well as practical application performance including skin irritation of laundry detergent formulated with 1227 was emphatically examined and compared with that of market product with similar functions. Results indicated that, in formulation without anionic surfactant, all indices of self-prepared bacteriostatic laundry detergent can meet requirements specified in relevant standards. The foaming power of self-prepared bacteriostatic laundry detergent was lower and easier to rinse than bacteriostatic laundry detergent product in market. Using aqueous solution of 1% mass fraction of self-prepared bacteriostatic laundry detergent containing 2%of 1227, the antibacterial rate for 20 min on both Escherichia coli and Staphylococcus aureus separately reached 100%, which was much better than that of the bacteriostatic laundry detergent product from market. The action effectiveness of the self-prepared bacteriostatic laundry detergent can be maintained for a longer time period and shows no obvious irritation effect to human skin.展开更多
基金Supported by Application Development Research Project of Chongqing Municipal Commission of Science and Technology in 2014 "Occurrence Regularity and Integrated Key Control Technologies of Kiwifruit Bacterial Canker"(cstc2014yykfA8011)
文摘[ Objective ] The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [ Method ] Ten kinds of com- mon commercial bactericides preliminarily screened were selected for indoor antibacterial test using paper plate method and plate streaking method, and six kinds of bactericides with good antibacterial effects were further screened for indoor toxicity test. [ Result] Chloramphenicoloethylicin had the best antibacterial effect, with EC50 of 36.34 rag/L; followed by Xintianxlucai No. 6 and embamycin, with EC50 of 83.21 and 92.34 mg/L, respectively. [ Conclusion] Cldoramphenicoloethylicin, Xintianxiucai No. 6 and embamycin screened in the test had good antibacterial effect against kiwifruit bacteriai canker in Wanzhou District, and chlorampheni- coleethylicin was the most effective agent. These three agents could be directly applied to control kiwifruit bacterial canker in production, while application time and climate conditions in fields should also be considered during the actual application process. It is suggested that three agents can be used alternatively in order to de- lay the resistance of plants.
基金Supported by Project for Modern Agro-industry Technology Research System(CARS-12-hnhgx)Sub-project of National Science and Technology Support Program(2007BAD48B06)Fundamental Research Special for Central-level Nonprofit Research Institutes(2011hzs1J024,2012hzs1J027)
文摘The toxicity of 12 kinds of bactericides against the pathogen of cassava bacterial blight （Xanthomonas axonopodis pv. manihotis） was determined by tox- ic medium method. The results showed that 2% triadimefon · 30% ethylicin EC, 80% ethylicin EC, 70% mancozeb WP and 72% strepolin SP had the lowest ef- fective concentration and the best inhibitory effect, while 12.5% myclobutanil SP, 20% thiodiazole-copper SC and 20% bismerthlazol WP had the highest effective concentration but the worst inhibitory effect. Field test of six kinds of bactericides was conducted in Zhanjiang City of Guangdong Province in 2011, and the results showed that 3% zhongshengrnycin WP, 2% triadimefon · 30% ethylicin EC and 80% ethylicin EC had good control effect, which were suggested to be used for field control.
基金supported by the“Ju Yuan Xing Chuan”Project of Sichuan Province(2022ZHCG0128).
文摘Protease-assisted soaking has received increasing attention in recent years.However,few reports have elaborated on the effect of bactericides,which are used to protect raw hides from microbial damage in the soaking process,on the performance of protease-assisted soaking.Here we investigate the effects of three bactericides,namely,2-methyl-4-isothiazolin-3-one(MIT),sodium propyl 4-hydroxybenzoate(SPHB)and cetyl trimethyl ammonium bromide(CTAB),on the catalytic activity of protease.MIT and SPHB have little effect on the proteolytic activity,whilst CTAB has a negative effect.Fluorescence spectroscopy,synchronous fluorescence spectroscopy,molecular docking and molecular dynamics simulation were used to analyse the bactericide-protease interaction.The data reveal that MIT and SPHB are bound to the non-catalytic sites of protease,whilst CTAB affects the catalytic triad of protease.Furthermore,the protease and bactericides were used alone,simultaneously and sequentially in the soaking process,and their soaking performances were evaluated.The evaluation shows that the use of protease increases the microorganisms in the soaking float,and MIT exhibits the best bactericidal effect.The simultaneous use of protease and MIT effectively inhibits bacteria and scarcely affects the removal of unstructured proteins from hides and the attack on epidermis by protease.These findings contribute to a better understanding of the scientific use of protease with other auxiliaries in soaking.
文摘Jasmine essential oil and lemon essential oil were selected for deodorizing ingredient.Lysine and silver ions were selected as auxiliary raw material.The product was prepared by emulsion dissolution technology.The minimum inhibitory concentration of various bacteria were during 100~2,000 mg/L.In the test,the germicidal efficiency of 2.0%(w/w)deodorant was 99%.The removal efficiency of trimethylamine and methanthiol were more than 95%by 3.0%(w/w).deodorant.The removal efficiency of ammonia and methanthiol were more than 92%by 3.0%(w/w)deodorant.In addition,The removal efficiency of isovaleric acid was 98%by 2.0%(w/w)deodorant.In analysis result,common pathogenic bacteria were effectively suppressed,kitchen and toilet odors were efficiently removed by natural plant deodorant.
基金supported by the National Natural Science Foundation of China(No.31972802)the Natural Science Foundation of Shandong Province(No.ZR2019MC041)the Taishan Scholar Program of Shandong Province(No.tsqn201812023).
文摘In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.
基金support from the National Natural Science Foundation of China(No.52175187)the Fundamental Research Funds for the Central Universities(No.3102019JC001)the Research Fund of the State Key Laboratory of Solidification Processing(NPU),China(No.2023-TS-06).
文摘Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for catheter coating materials.Photothermal therapy(PTT)is a highly efficient bactericidal method.Here,a composite hydrogel containing MXene nanosheets and hydrophilic 3-sulfopropyl methacrylate potassium salt(SPMK)is reported,which is synthesized through the one-pot method and heat-initiated polymerization.The hydrogel shows excellent antibacterial performance against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)in 3 min in the air or 20 min in the water environment under near-infrared light(NIR;808 nm)irradiation.The friction coefficient of the hydrogel is about 0.11,which is 48%lower than that without SPMK.The rapid photothermal sterilization is attributed to the outstanding antibacterial ability and thermal effect of photoactivated MXene.The ultra-low friction is the result of the hydration lubrication mechanism.This study provides a potential strategy for the surface coatings of biomedical catheters,which enables rapid sterilization and extremely low interface resistance between catheters and biological tissues.
基金supported by National Natural Science Foundation of China(No.51977174)。
文摘Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive species,which can be regulated by the activation process.In this study,surface plasma-activated air(SAA)and a He+O_(2)plasma jet(Jet)were parallelly combined(the SAA+Jet combination)or sequentially combined(the SAA→Jet combination and the Jet→SAA combination)to prepare plasma-activated saline(PAS).The PAS activated by the combinations exhibited stronger bactericidal effects than that activated by the SAA or the Jet alone.The concentrations of H_(2)O_(2)and NO_(2)^(-)were higher in the PAS activated by the Jet→SAA combination,while ONOO^(-)concentrations were close in the three kinds of PAS and^(1)O_(2)concentrations were higher in the PAS activated by the SAA+Jet combination.The analysis of scavengers also demonstrated that H_(2)O_(2),^(1)O_(2),and ONOO^(-)in the PAS activated by the SAA+Jet combination,and^(1)O_(2)in the PAS activated by the Jet→SAA combination played critical roles in bactericidal effects.Further,the effective placement time of the three PAS varied,and the PAS activated by the Jet→SAA combination could also inactivate 2.6-log_(10)of MRSA cells after placement for more than 60 min.The regulation of reactive species in plasma-activated water via different combinations of plasma devices could improve the directional application of plasma-activated water in the biomedical field.
基金supported by the Biological Breeding-Major Projects(2023ZD04065)。
文摘Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.
文摘Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 2022. Methodology: During this eleven-month prospective study, we set up a quality control device (QCD). For microbiological quality control, we performed different cultures of bacteria with different bacteriological and morphological characteristics at T0 (no sterilization) and at T30 (after 30 minutes of sterilization under DISTER-UV). Results: After the realization, the DCQ attached to the DISTER-UV1 allows to display of the UV wavelength present in the light box. This device also displays and alerts when the UV intensity emitted by the lamps is below 250 nm. During microbiological quality control, the cultures carried out at T30 and incubated for 24 hours did not reveal any bacterial colonies. This shows the bactericidal character of DISTER-UV-2. Conclusion: The improvement and the microbiological quality control allowed us to switch from DISTER-UV1 (without sensor) to DISTER-UV-2 (with sensor or DCQ). The biological control allowed us to affirm that the DISTER-UV-2 is bactericidal.
基金funded by the Forestry Science and Technology Innovation Project of Guangdong Province,China(2020KJCX010).
文摘This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family.The inhibitory rate,minimum inhibitory concentration(MIC),and minimum bactericidal concentration(MBC)of leaf and stem,and root and rhizome extracts from Alpinia katsumadai Hayata,Alpinia oxyphylla Miq×Alpinia henryi K.Schumann,Alpinia oblongifolia Hayata,Alpinia nigra(Gaertn.)Burtt,Amomum villosum Lour,Alpinia zerumbet(Pers.)Burtt.et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method.The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi.At a 1.5 mg mL^(−1),A.zerumbet root and rhizome extracts exhibited strong inhibitory activity against S.aureus and E.coli,with 83.23%and 79.62%,respectively.In addition,A.zerumbet leaf and stem extracts had an inhibitory rate of 90.85%against P.aeruginosa.At the same concentration,the leaf and stem,root and rhizome extracts of A.katsumadai had the best anti-bacterial effect against F.oxysporum,with inhibition rates of 84.46%and 84.73%,respectively.Moreover,A.katsumadai and A.zerumbet leaf and stem extracts had the most significant antibacterial effect against S.aureus,with a MIC of 0.063 mg mL^(−1).Thus,both A.katsumadai and A.zerumbet extracts had significant antibacterial activity.In addition,by comparing the inhibitory effect of extracts from different parts,it was found that the inhibitory rate and average inhibitory rate of extracts from leaf and stem were higher than those from root and rhizome.The chemical constituents of A.katsumadai and A.zerumbet,determined by the high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),revealed that citric acid(CA),alpinetin,and pinocembrin(PNCB)were the functional constituents yielding the antibacterial activity.Overall,A.katsumadai and A.zerumbet have the potential to be developed as new plant fungicides and bactericides.
文摘The determination of 7 bactericidals residues,including chlorthalonil,dichlofluanid,tolylfluanid,captan,folpet,captafol and deltamethrin,in foods by using on-line gel permeation chromatography(GPC) coupled with gas chromatography-mass spectrometry(GC-MS).Bactericidal residues were extracted with acetonitrile,and cleaned up by ENVI-Crab,LC-NH2 column and GPC,respectively.The analysis of 7 bactericidals residues was performed by GC-MS.The average recoveries of analytes were range of 75%-120% with RSD of 3.5%-10.3%.
基金Supported by National Key Research and Development Program of China(Grant No.2020YFB2010500)National Natural Science Foundation of China(Grant Nos.51975305,51905289)+2 种基金Shandong Provincial Major Science and Technology Innovation Engineering Projects of China(Grant No.2019JZZY020111)Shandong Provincial Natural Science Foundation of China(Grant Nos.ZR2020KE027,ZR2020ME158,ZR2019PEE008)Applied Basic Research Youth Project of Qingdao Science and Technology Plan(Grant No.19-6-2-63-cg)。
文摘The application of cutting fluid in the field of engineering manufacturing has a history of hundreds of years,and it plays a vital role in the processing efficiency and surface quality of parts.Among them,water-based cutting fluid accounts for more than 90%of the consumption of cutting fluid.However,long-term recycling of water-based cutting fluid could easily cause deterioration,and the breeding of bacteria could cause the cutting fluid to fail,increase manufacturing costs,and even endanger the health of workers.Traditional bactericides could improve the biological stability of cutting fluids,but they are toxic to the environment and do not conform to the development trend of low-carbon manufacturing.Low-carbon manufacturing is inevitable and the direction of sustainable manufacturing.The use of nanomaterials,transition metal complexes,and physical sterilization methods on the bacterial cell membrane and genetic material could effectively solve this problem.In this article,the mechanism of action of additives and microbial metabolites was first analyzed.Then,the denaturation mechanism of traditional bactericides on the target protein and the effect of sterilization efficiency were summarized.Further,the mechanism of nanomaterials disrupting cell membrane potential was discussed.The effects of lipophilicity and the atomic number of transition metal complexes on cell membrane penetration were also summarized,and the effects of ultraviolet rays and ozone on the destruction of bacterial genetic material were reviewed.In other words,the bactericidal performance,hazard,degradability,and economics of various sterilization methods were comprehensively evaluated,and the potential development direction of improving the biological stability of cutting fluid was proposed.
文摘ByBimolecular Nucleophilic Substitution, four new types of alkylene triphenyl double quaternary phosphonium salt were synthesized respectively by using triphenylphosphine, 1,3-dibromopropane, 1,6-dibromohexane,1,10-dibromo- decane, 1,12-dibromododecane as raw materials and using DMAC as the solvent, under a certain temperature and reac- tion time. The productivity is 58% - 83%. The molecular structures of the products were characterized by IR, NMR and elemental analysis. The sterilizing effect of 1,6-hexylidene triphenyl double phosphonium bromide(HTDPB) and 1,12- dodecylidene triphenyl double phosphonium bromide(DoTDPB) was evaluated by using extinct dilution method.The experimental result shows that the sterilizing effect of DoTDPB is better than the effect of HTDPB under the same drug concentration and contact time. When the concentration of DoTDPB was 20 mg/L and the contact time was 0.5 h, the sterilizing rate of DoTDPB used to kill saprophytic bacteria (TGB), sulfate-reducing bacteria (SRB) and iron bacteria (IB) was 95.56%, 84% and 99.58% respectively.
文摘By way of bactericidal halo test, the antibacterial performance of various electroplated deposits (cobalt, zinc, copper, silver, gold, nickel, chromium, cobalt-containing alloy of nickel, and nickel-containing alloy of cobalt) were determined. The results of the test show that the electroplated deposits of zinc, cobalt, cobalt-containing alloy of nickel, and nickel-containing alloy of cobalt, silver and copper possess a distinct antibacterial capability against the growth of pathogenic bacteria tested, and that SUS304 stainless steel and chromium deposit have no antibacterial activity. The relationship between the antibacterial capability and the rest potentials of the deposits was investigated. It was found that the higher the antibacterial activity of the electroplated deposit was, the more negative the rest potential of deposit was.
文摘This work is a result of previously done studies on the synthesis of A2FeVIO4 wet ferrate (VI) formula, using chlorine as an oxidant. The major problem of these ferrates is related to their stability over time. This brings us to identify and optimize the critical parameters influencing the preparation of the Na2FeO4 at room stable phase with acceptable performance. The use of water bleach (hypochlorite ClO‾) at a chlorometric degree of 50°F in the synthesis of the Na2FeO4 ambient stable phase promotes the oxidation of iron (II) iron to (VI) in a concentrated NaOH alkaline medium. The synthesis reaction is in the presence of FeSO4 7H2O hydrated iron sulfate at a temperature of about 55°C in order to simplify the synthesis process, to enhance the production of the Fe (VI) and to meet the growing demand of ferrates (VI) for their interest in the treatment of water. Monitoring the degradation of synthesized Na2FeO4 shows its stability up to 12 months, which facilitates storage and transportation. The phases obtained were characterized by IR spectroscopy, and RX by UV spectrophotometer, measuring the optical density at 507 nm.
文摘Quaternary ammonium compound are a class of broad-spectrum,high-efficiency and low-toxicity bactericide,which are widely used in daily life and industrial sterilization.Herein,the current state of domestic quaternary ammonium disinfectant products was summarized,as well as those new products developed and studied,and the development trend was also predicted.
文摘The iron compounds in the oxidation state (VI) have the specific advantage of being powerful oxidants and bactericides. This feature explains their particular interest in the treatment of water. The aim of this work is to prepare Na2FeO4 stable at ambient in order to optimize the key parameters influencing the performance of the oxidation of iron (II) to iron (VI), as well as to monitor its degradation over time. The synthesis of this phase has been carried out by using the dry reaction Na2O2 with Fe2O3 with a temperature of 700°C for a reaction time of 13 hours with a Na/Fe ratio of 4 to make it possible to simplify the synthesis procedure, to minimize the cost and enhance the production of iron (VI) to meet the growing demand of ferrate (VI) for its interest in water treatment. The obtained phase was characterized by UV spectrophotometer by measuring the optical density at a wavelength of 507 nm.
文摘Essential oils (EOs) are natural bioactive compounds with antibacterial activity against a variety of microorganisms including phytopathogens. The use of EOs and their components as viable therapeutic antibacterials is however greatly compromised by their volatile nature, hydrophobicity and instability when exposed to environmental and physiological factors. Encapsulation of these compounds in an appropriate carrier system can alleviate these challenges. This study therefore aimed at developing, characterizing and evaluating the efficacy of antibacterial potential of thymol and eugenol loaded chitosan nanoparticles (TCNPs and ECNPs) against <i>Ralstonia solanacearum</i>, the bacterial wilt-causing pathogen in potatoes. Synthesis of TCNP and ECNP was achieved via ionic gelation method and the prepared nanoparticles characterized by their particle size distributions, encapsulation efficiency, loading capacity (LC) and <i>in-vitro</i> release characteristics. Antibacterial activities of the nanoparticles were investigated using agar dilution and colony counting methods and their minimum inhibitory concentration (MIC) determined by 96-well broth micro-dilution method. Scanning electron microscope images of TCNPs and ECNPs showed that the nanoparticles were spherical in shape and were well separated with an average particle size of 590 nm and 555 nm respectively. The average size of chitosan nanoparticles alone was however 375 nm. The encapsulation efficiency was 72.9% for TCNP (with 48.3% LC) and 71.7% for ECNP (with 49.5% LC). The release of thymol and eugenol from the microcapsules was found to be pH dependent with the highest release at pH 1.5. The growth inhibition of <i>R. solanacearum</i> was 92% and 94% for TCNP and ECNPs respectively. The MIC of thymol and eugenol before encapsulation was 175 μg/ml and 275 μg/ml respectively, but this reduced significantly to 22.5 μg/ml and 45 μg/ml after encapsulation. Thus, encapsulation of thymol and eugenol in chitosan nanoparticles has shown promising potential as a bactericide alternative for <i>R. solanacearum</i> and could be useful in managing the soil borne phytopathogen.
文摘Aryloxy--terminated polydimethylsiloxanes (AOS) were synthesized by the telomerization of hexamethylcyclotrisiloxane(D3) with phenols. The structures of AOS were characterized by UV, IR, 2HNMR and elemental analysis, The effects of the reaction conditions and the mole ratios of the reactants on foe molecular weight of the polymer were discussed. A OS's structural influence on bactericidal action, flocculation and hydrolysis was studied.
文摘Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on sensory and physiochemical quality indices, detergency and foaming performance of laundry detergent were comprehensively investigated,and antimicrobial performance as well as practical application performance including skin irritation of laundry detergent formulated with 1227 was emphatically examined and compared with that of market product with similar functions. Results indicated that, in formulation without anionic surfactant, all indices of self-prepared bacteriostatic laundry detergent can meet requirements specified in relevant standards. The foaming power of self-prepared bacteriostatic laundry detergent was lower and easier to rinse than bacteriostatic laundry detergent product in market. Using aqueous solution of 1% mass fraction of self-prepared bacteriostatic laundry detergent containing 2%of 1227, the antibacterial rate for 20 min on both Escherichia coli and Staphylococcus aureus separately reached 100%, which was much better than that of the bacteriostatic laundry detergent product from market. The action effectiveness of the self-prepared bacteriostatic laundry detergent can be maintained for a longer time period and shows no obvious irritation effect to human skin.
