In this study,a novel porous 3D composite scaffold based on the biodegradable Poly(ε-caprolactone)(PCL),Polylactide Acid(PLA)and Calcium Citrate(CC)was developed via polymer blends and thermal-induced phase separatio...In this study,a novel porous 3D composite scaffold based on the biodegradable Poly(ε-caprolactone)(PCL),Polylactide Acid(PLA)and Calcium Citrate(CC)was developed via polymer blends and thermal-induced phase separation.The chemical structure,crystalline structure and micromorphology as well as mechanical strength of the scaffolds were characterized by Fourier Transform Infrared Spectroscopy(FTIR),X-ray Diffraction(XRD),Scanning Electron Microscope(SEM)and tensile tests.The results show that the obtained composite scaffold present a suitable bone-like porous structure and sufficient mechanical strength.Furthermore,the release of calcium ions in Simulated Body Fluid(SBF)indicates that the composite material can provide a stable calcium-ion environment and maintain a constant pH value during the soaking process.The cell proliferation results from CCK-8 and light microscopy show that MG63 cells exhibit excellent adhesion and proliferation on the stent.At the same time,animal implantation histology confirms that the composite scaffolds have good biocompatibility in vivo.The scaffold material has greatly potential application value in the field of bone tissue engineering.展开更多
Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totall...Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 （CC-rhBMP-2） group and rhBMP-2 only group. Two 10 ram-long and 5 ram-deep bone defects were respectively created in the left and right femoral condyles of the rabbits. Subsequently 5 pellets of calcium citrate （10 mg） combined with rhBMP-2 （2 rag） or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab. Bone granules were obtained at 2, 4 and 6 weeks after procedure and received histological analysis. LSD t-test and a subsequent t-test were adopted for statistical analysis. Results： Histomorphometric analysis revealed newlyformed bones, and calcium citrate has been absorbed in the treatment group. The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73%±1.26% vs 48.21%±2.37% at 2 weeks; 43.40%±1.65% vs 57.32%±1.47% at 4 weeks, and 51.32%±7.80% vs 66.74%±4.05% at 6 weeks （P〈0.05 for all）. At 2 weeks, mature cancellous bone was observed to be already formed in the treatment group. Conclusion： From this study, it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects. This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.展开更多
基金jointly supported by the National Natural Science Foundation of China(NO.41673109)Sichuan Science and Technology Program(2021YFH0098)Key Project of Sichuan Vanadium and Titanium Industry Development Research Center(2018VTCYZ-01).
文摘In this study,a novel porous 3D composite scaffold based on the biodegradable Poly(ε-caprolactone)(PCL),Polylactide Acid(PLA)and Calcium Citrate(CC)was developed via polymer blends and thermal-induced phase separation.The chemical structure,crystalline structure and micromorphology as well as mechanical strength of the scaffolds were characterized by Fourier Transform Infrared Spectroscopy(FTIR),X-ray Diffraction(XRD),Scanning Electron Microscope(SEM)and tensile tests.The results show that the obtained composite scaffold present a suitable bone-like porous structure and sufficient mechanical strength.Furthermore,the release of calcium ions in Simulated Body Fluid(SBF)indicates that the composite material can provide a stable calcium-ion environment and maintain a constant pH value during the soaking process.The cell proliferation results from CCK-8 and light microscopy show that MG63 cells exhibit excellent adhesion and proliferation on the stent.At the same time,animal implantation histology confirms that the composite scaffolds have good biocompatibility in vivo.The scaffold material has greatly potential application value in the field of bone tissue engineering.
文摘Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 （CC-rhBMP-2） group and rhBMP-2 only group. Two 10 ram-long and 5 ram-deep bone defects were respectively created in the left and right femoral condyles of the rabbits. Subsequently 5 pellets of calcium citrate （10 mg） combined with rhBMP-2 （2 rag） or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab. Bone granules were obtained at 2, 4 and 6 weeks after procedure and received histological analysis. LSD t-test and a subsequent t-test were adopted for statistical analysis. Results： Histomorphometric analysis revealed newlyformed bones, and calcium citrate has been absorbed in the treatment group. The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73%±1.26% vs 48.21%±2.37% at 2 weeks; 43.40%±1.65% vs 57.32%±1.47% at 4 weeks, and 51.32%±7.80% vs 66.74%±4.05% at 6 weeks （P〈0.05 for all）. At 2 weeks, mature cancellous bone was observed to be already formed in the treatment group. Conclusion： From this study, it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects. This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.
