期刊文献+
共找到2,056篇文章
< 1 2 103 >
每页显示 20 50 100
Dietary sodium acetate and sodium butyrate improve high-carbohydrate diet utilization by regulating gut microbiota, liver lipid metabolism, oxidative stress, and inflammation in largemouth bass(Micropterus salmoides) 被引量:1
1
作者 Qiao Liu Liangshun Cheng +9 位作者 Maozhu Wang Lianfeng Shen Chengxian Zhang Jin Mu Yifan Hu Yihui Yang Kuo He Haoxiao Yan Liulan Zhao Song Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1704-1722,共19页
Background Adequate level of carbohydrates in aquafeeds help to conserve protein and reduce cost. However, studies have indicated that high-carbohydrate(HC) diet disrupt the homeostasis of the gut–liver axis in large... Background Adequate level of carbohydrates in aquafeeds help to conserve protein and reduce cost. However, studies have indicated that high-carbohydrate(HC) diet disrupt the homeostasis of the gut–liver axis in largemouth bass, resulting in decreased intestinal acetate and butyrate level.Method Herein, we had concepted a set of feeding experiment to assess the effects of dietary sodium acetate(SA) and sodium butyrate(SB) on liver health and the intestinal microbiota in largemouth bass fed an HC diet. The experimental design comprised 5 isonitrogenous and isolipidic diets, including LC(9% starch), HC(18% starch), HCSA(18% starch;2 g/kg SA), HCSB(18% starch;2 g/kg SB), and HCSASB(18% starch;1 g/kg SA + 1 g/kg SB). Juvenile largemouth bass with an initial body weight of 7.00 ± 0.20 g were fed on these diets for 56 d.Results We found that dietary SA and SB reduced hepatic triglyceride accumulation by activating autophagy(ATG101, LC3B and TFEB), promoting lipolysis(CPT1α, HSL and AMPKα), and inhibiting adipogenesis(FAS, ACCA, SCD1 and PPARγ). In addition, SA and SB decreased oxidative stress in the liver(CAT, GPX1α and SOD1) by activating the Keap1-Nrf2 pathway. Meanwhile, SA and SB alleviated HC-induced inflammation by downregulating the expression of pro-inflammatory factors(IL-1β, COX2 and Hepcidin1) through the NF-κB pathway. Importantly, SA and SB increased the abundance of bacteria that produced acetic acid and butyrate(Clostridium_sensu_stricto_1). Combined with the KEGG analysis, the results showed that SA and SB enriched carbohydrate metabolism and amino acid metabolism pathways, thereby improving the utilization of carbohydrates. Pearson correlation analysis indicated that growth performance was closely related to hepatic lipid deposition, autophagy, antioxidant capacity, inflammation, and intestinal microbial composition.Conclusions In conclusion, dietary SA and SB can reduce hepatic lipid deposition;and alleviate oxidative stress and inflammation in largemouth bass fed on HC diet. These beneficial effects may be due to the altered composition of the gut microbiota caused by SA and SB. The improvement effects of SB were stronger than those associated with SA. 展开更多
关键词 High carbohydrate diet Intestinal microbiota Largemouth bass Lipid deposition Sodium acetate Sodium butyrate
下载PDF
Understanding activity of butyrate at a cellular level
2
作者 Prapti Chakraborty Angela S.Laird 《Neural Regeneration Research》 SCIE CAS 2025年第8期2323-2324,共2页
Butyrate is a short-chain fatty acid of four carbons in length that is a by-product produced by the microbial fermentation of dietary fiber and undigested carbohydrates within the colon.Over the years,butyrate has att... Butyrate is a short-chain fatty acid of four carbons in length that is a by-product produced by the microbial fermentation of dietary fiber and undigested carbohydrates within the colon.Over the years,butyrate has attracted significant attention due to its diverse roles within cells. 展开更多
关键词 CARBONS butyrATE FIBER
下载PDF
Sodium butyrate alleviates fructose-induced non-alcoholic fatty liver disease by remodeling gut microbiota to promoteγ-amino butyric acid production
3
作者 Qu Chen Lei Wu +4 位作者 Aijia Zhang Chen Wu Liuping Cai Yingping Xiao Yingdong Ni 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期961-971,共11页
Sodium butyrate(NaB)can regulate lipid metabolism and inhibit hepatic steatosis.This study aimed to investigate whether NaB can alleviate fructose-induced hepat ic steatosis via remodeling the gut microbiota and evalu... Sodium butyrate(NaB)can regulate lipid metabolism and inhibit hepatic steatosis.This study aimed to investigate whether NaB can alleviate fructose-induced hepat ic steatosis via remodeling the gut microbiota and evaluate the anti-fatty liver mechanisms.The results showed that NaB and NaB-remodeled gut microbiota significantly alleviated fructose-induced hepatic steatosis and increased plasma uric acid and fructose levels.Furthermore,both NaB and NaB-remodeled gut microbiota increased the abundance of Lactobacillus and altered the levels of plasma amino acids(upregulating gamma-amino butyric acid(GABA)and downregulating L-glutamic acid and L-arginine)in fructose-exposed mice.The correlation analysis showed that GABA levels positively correlated with Lactobacillus abundance,and increased GABA levels might promote the reduction of the hepatic triglyceride content.Further studies confirmed that GABA significantly reduced lipid deposition in mouse hepatocytes induced via fructose pretreatment in vitro.These findings suggested that NaB could ameliorate fructose-induced hepatic steatosis by regulating gut microbiota. 展开更多
关键词 butyrATE FRUCTOSE Gut microbiota Hepatic steatosis
下载PDF
Alginate oligosaccharide-mediated butyrate-HIF-1α axis improves skin aging in mice
4
作者 Ting Gao Yixuan Li +1 位作者 Xiaoyu Wang Fazheng Ren 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第5期678-692,共15页
The“gut-skin”axis has been proved and is considered as a novel therapy for the prevention of skin aging.The antioxidant efficacy of oligomannonic acid(MAOS)makes it an intriguing target for use to improve skin aging... The“gut-skin”axis has been proved and is considered as a novel therapy for the prevention of skin aging.The antioxidant efficacy of oligomannonic acid(MAOS)makes it an intriguing target for use to improve skin aging.The present study further explored whereby MAOS-mediated gut-skin axis balance prevented skin aging in mice.The data indicated the skin aging phenotypes,oxidative stress,skin mitochondrial dysfunction,and intestinal dysbiosis(especially the butyrate and HIF-1a levels decreased)in aging mice.Similarly,fecal microbiota transplantation(FMT)from aging mice rebuild the aging-like phenotypes.Further,we demonstrated MAOS-mediated colonic butyrate-HIF-1a axis homeostasis promoted the entry of butyrate into the skin,upregulated mitophagy level and ultimately improving skin aging via HDAC3/PHD/HIF-1a/mitophagy loop in skin of mice.Overall,our study offered a better insights of the effectiveness of alginate oligosaccharides(AOS),promised to become a personalized targeted therapeutic agents,on gut-skin axis disorder inducing skin aging. 展开更多
关键词 Alginate oligosaccharide Skin aging butyrATE HIF-1A MITOPHAGY
下载PDF
Coated sodium butyrate ameliorates high‑energy and low‑protein diet induced hepatic dysfunction via modulating mitochondrial dynamics, autophagy and apoptosis in laying hens
5
作者 Sasa Miao Tianming Mu +5 位作者 Ru Li Yan Li Wenyan Zhao Jiankui Li Xinyang Dong Xiaoting Zou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第3期1190-1206,共17页
Background Fatty liver hemorrhagic syndrome(FLHS),a fatty liver disease in laying hens,poses a grave threat to the layer industry,stemming from its ability to trigger an alarming plummet in egg production and usher in... Background Fatty liver hemorrhagic syndrome(FLHS),a fatty liver disease in laying hens,poses a grave threat to the layer industry,stemming from its ability to trigger an alarming plummet in egg production and usher in acute mortality among laying hens.Increasing evidence suggests that the onset and progression of fatty liver was closely related to mitochondria dysfunction.Sodium butyrate was demonstrated to modulate hepatic lipid metabolism,alle-viate oxidative stress and improve mitochondrial dysfunction in vitro and mice models.Nevertheless,there is limited existing research on coated sodium butyrate(CSB)to prevent FLHS in laying hens,and whether and how CSB exerts the anti-FLHS effect still needs to be explored.In this experiment,the FLHS model was induced by administering a high-energy low-protein(HELP)diet in laying hens.The objective was to investigate the effects of CSB on alleviating FLHS with a focus on the role of CSB in modulating mitochondrial function.Methods A total of 288 healthy 28-week-old Huafeng laying hens were arbitrarily allocated into 4 groups with 6 replicates each,namely,the CON group(normal diet),HELP group(HELP diet),CH500 group(500 mg/kg CSB added to HELP diet)and CH750 group(750 mg/kg CSB added to HELP diet).The duration of the trial encompassed a period of 10 weeks.Results The result revealed that CSB ameliorated the HELP-induced FLHS by improving hepatic steatosis and patho-logical damage,reducing the gene levels of fatty acid synthesis,and promoting the mRNA levels of key enzymes of fatty acid catabolism.CSB reduced oxidative stress induced by the HELP diet,upregulated the activity of GSH-Px and SOD,and decreased the content of MDA and ROS.CSB also mitigated the HELP diet-induced inflammatory response by blocking TNF-α,IL-1β,and F4/80.In addition,dietary CSB supplementation attenuated HELP-induced activation of the mitochondrial unfolded protein response(UPRmt),mitochondrial damage,and decline of ATPase activity.HELP diet decreased the autophagosome formation,and downregulated LC3B but upregulated p62 protein expression,which CSB administration reversed.CSB reduced HELP-induced apoptosis,as indicated by decreases in the Bax/Bcl-2,Caspase-9,Caspase-3,and Cyt C expression levels.Conclusions Dietary CSB could ameliorate HELP diet-induced hepatic dysfunction via modulating mitochondrial dynamics,autophagy,and apoptosis in laying hens.Consequently,CSB,as a feed additive,exhibited the capacity to prevent FLHS by modulating autophagy and lipid metabolism. 展开更多
关键词 AUTOPHAGY Coated sodium butyrate Laying hens Lipid metabolism MITOCHONDRIA
下载PDF
Fusobacterium nucleatum-induced imbalance in microbiome-derived butyric acid levels promotes the occurrence and development of colorectal cancer
6
作者 Qi-Long Wu Xiao-Ting Fang +5 位作者 Xin-Xin Wan Qing-Yong Ding Yan-Jun Zhang Ling Ji Yong-Liang Lou Xiang Li 《World Journal of Gastroenterology》 SCIE CAS 2024年第14期2018-2037,共20页
BACKGROUND Colorectal cancer(CRC)ranks among the most prevalent malignant tumors globally.Recent reports suggest that Fusobacterium nucleatum(F.nucleatum)contributes to the initiation,progression,and prognosis of CRC.... BACKGROUND Colorectal cancer(CRC)ranks among the most prevalent malignant tumors globally.Recent reports suggest that Fusobacterium nucleatum(F.nucleatum)contributes to the initiation,progression,and prognosis of CRC.Butyrate,a short-chain fatty acid derived from the bacterial fermentation of soluble dietary fiber,is known to inhibit various cancers.This study is designed to explore whether F.nucleatum influences the onset and progression of CRC by impacting the intestinal metabolite butyric acid.AIM To investigate the mechanism by which F.nucleatum affects CRC occurrence and development.METHODS Alterations in the gut microbiota of BALB/c mice were observed following the oral administration of F.nucleatum.Additionally,DLD-1 and HCT116 cell lines were exposed to sodium butyrate(NaB)and F.nucleatum in vitro to examine the effects on proliferative proteins and mitochondrial function.RESULTS Our research indicates that the prevalence of F.nucleatum in fecal samples from CRC patients is significantly greater than in healthy counterparts,while the prevalence of butyrate-producing bacteria is notably lower.In mice colonized with F.nucleatum,the population of butyrate-producing bacteria decreased,resulting in altered levels of butyric acid,a key intestinal metabolite of butyrate.Exposure to NaB can impair mitochondrial morphology and diminish mitochondrial membrane potential in DLD-1 and HCT116 CRC cells.Consequently,this leads to modulated production of adenosine triphosphate and reactive oxygen species,thereby inhibiting cancer cell prolif-eration.Additionally,NaB triggers the adenosine monophosphate-activated protein kinase(AMPK)signaling pathway,blocks the cell cycle in HCT116 and DLD-1 cells,and curtails the proliferation of CRC cells.The combined presence of F.nucleatum and NaB attenuated the effects of the latter.By employing small interfering RNA to suppress AMPK,it was demonstrated that AMPK is essential for NaB’s inhibition of CRC cell proliferation.CONCLUSION F.nucleatum can promote cancer progression through its inhibitory effect on butyric acid,via the AMPK signaling pathway. 展开更多
关键词 Colorectal cancer Fusobacterium nucleatum butyric acid Gut microbiota Adenosine monophosphate-activated protein kinase signal pathway
下载PDF
Effects of coagulation bath temperature on structure and performance of poly(vinyl butyral) hollow fiber membranes via thermally induced phase separation 被引量:2
7
作者 张明 邱运仁 《Journal of Central South University》 SCIE EI CAS 2014年第8期3057-3062,共6页
Poly (vinyl butyral) (PVB) hollow fiber membranes were fabricated via thermally induced phase separation (TIPS). The effects of coagulation bath temperature (CBT) on the structure and performance of membranes ... Poly (vinyl butyral) (PVB) hollow fiber membranes were fabricated via thermally induced phase separation (TIPS). The effects of coagulation bath temperature (CBT) on the structure and performance of membranes were investigated in detail. The morphologies of the membranes were studied by scanning electron microscopy (SEM), the performances of water permeability, rejection, breaking strength and elongation were measured, respectively. The results indicate that all the membranes have the asymmetric morphology and the thickness of the skin layer decreases and the pore size of the outer layer increases with the increase of CBT. The permeability of membranes prepared at air gap 1.0 cm and take-up speed 0.253 m/s increases from 1.047×10-7 to 5.909×10-7 m3/(m2·s-kPa) with the CBT increasing from 20 ℃ to 40℃, and sharply increases to 35.226×10 7 m3/(m2.s.kPa)once the CBT arrives at 50 ℃. While the carbonic ink rejections have no significant decrease, totally exceed 98%, but that of acid-maleic acid copolymer greatly decreases with the increase of CBT. Both the breaking strength and elongation decrease with the increase of CBT. 展开更多
关键词 thermally induced phase separation coagulation bath temperature poly (vinyl butyral hollow fiber membrane
下载PDF
Rheological properties of polyvinyl butyral/Pluronic F127/PEG200 blend systems
8
作者 欧阳伟 邱运仁 《Journal of Central South University》 SCIE EI CAS 2011年第6期1891-1896,共6页
The influence of temperature and mass fraction of Pluonic F127 on the rheological properties of polyvinyl butyric (PVB)/Pluronic F127/polyethylene glycol (PEG) 200 blend systems was investigated by a rotational rh... The influence of temperature and mass fraction of Pluonic F127 on the rheological properties of polyvinyl butyric (PVB)/Pluronic F127/polyethylene glycol (PEG) 200 blend systems was investigated by a rotational rheometer with parallel plates. The results show that the blend systems approach homogeneous state at 140, 150 and 160℃while the rheological properties of the blend systems significantly deviate from the homogeneous systems at 120℃. Shear thinning behavior is observed for all the blend systems at different temperatures and is enhanced by increasing Pluronic F127 content. The complex viscosity, storage modulus, loss modulus, zero-shear activation energy and flow recovery of the blend systems increase with the increase of Pluronic F127 content. There is a crossing point for the loss modulus and the storage modulus of each sample at high frequency, which is called specified frequency (SF). The loss modulus is larger than the storage modulus when the frequency is less than the SF, and when frequency is more than SF, the loss modulus is smaller than the storage modulus. 展开更多
关键词 polyvinyl butyral amphiphilic copolymer polyethylene glycol dynamic rheological properties
下载PDF
Electrokinetic phenomena of poly(vinyl butyral) hollow fiber membranes in different electrolyte solutions
9
作者 漆静 王永前 邱运仁 《Journal of Central South University》 SCIE EI CAS 2013年第6期1490-1495,共6页
The streaming potential of poly(vinyl butyral) (PVB) hollow fiber membrane was studied in different electrolyte solutions (including NaCl, KCl, CaCl2 and MgCl2), the effects of ionic strength, ion valence and pH... The streaming potential of poly(vinyl butyral) (PVB) hollow fiber membrane was studied in different electrolyte solutions (including NaCl, KCl, CaCl2 and MgCl2), the effects of ionic strength, ion valence and pH value on the streaming potential (SP) of the membrane were investigated. The zeta potentials and surface charge densities of the membrane were estimated on the basis of Helmholtz-Smoluchowski equation and Gouy-Chapmann theory. The results show that the PVB membrane has a weak negative charge due to the specific adsorption of ions. Moreover, the streaming potential, the zeta potential and the surface charge density of the membrane depend strongly on the salt concentration and the type and valence of ions. The iso-electric point (IEP) of the PVB membrane is arotmd 3.0 in the monovalent media (NaC1 and KC1) and 3.5 in divalent electrolytes (CaCl2 and MgCl2). A few retentions were obtained for PVB membrane in low concentration solutions. This result verifies that the negative charged membrane surface can reject inorganic solutes by means of electrostatic repulsion effect even though the size of membrane pores is much larger than the size of salts. 展开更多
关键词 streaming potential zeta potential poly(vinyl butyral hollow fiber membrane
下载PDF
Preparation of Cellulose Acetate Butyrate Porous Micro/Nanofibrous Membranes and Their Properties 被引量:1
10
作者 张晓晓 苏亚洲 +4 位作者 石凌翔 王玉洁 黄长芬 王新厚 孙晓霞 《Journal of Donghua University(English Edition)》 CAS 2023年第5期461-466,共6页
Cellulose acetate butyrate(CAB)is a cellulose ester that is commonly used in applications such as coatings and leather brighteners.However,its appearance in a fibrous form is rarely reported.CAB porous micro/nanofibro... Cellulose acetate butyrate(CAB)is a cellulose ester that is commonly used in applications such as coatings and leather brighteners.However,its appearance in a fibrous form is rarely reported.CAB porous micro/nanofibrous membranes with a large number of nanopores on the fiber surface were successfully prepared by electrospinning with dichloromethane(DCM)/acetone(AC)as the mixed solvent.Apparent morphology,porosity,moisture permeability,air permeability,static water contact angles,and thermal conductivity of the fibrous membranes were investigated at different spinning voltages.The results showed that with the increase of the spinning voltage,the average fiber diameter of the CAB porous micro/nanofibrous membranes gradually decreased and the fiber diameter distribution was more uniform.When the spinning voltage reached 40 kV,the porosity reached 91.38%,the moisture permeability was up to 7430 g/(m^(2)·d),the air permeability was up to 36.289 mm/s,the static water contact angle was up to 145.0°,while the thermal conductivity of the fibrous membranes reached 0.030 W/(m·K).The material can be applied as thermal-insulation,waterproof and moisture-permeable membranes. 展开更多
关键词 ELECTROSPINNING cellulose acetate butyrate(CAB) porous material waterproof and permeable membrane low thermal conductivity
下载PDF
Sodium butyrate alleviates deoxynivalenol-induced hepatic cholesterol metabolic dysfunction via RORγ-mediated histone acetylation modification in weaning piglets
11
作者 Qiufang Zong Huan Qu +5 位作者 Yahui Zhao Haoyu Liu Shenglong Wu Shuai Wang Wenbin Bao Demin Cai 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第2期792-805,共14页
Background:Cholesterol is an essential component of lipid rafts in cell plasma membrane,which exerts a hepatoprotective role against mycotoxin exposure in pigs,and cholesterol metabolism is vulnerable to epigenetic hi... Background:Cholesterol is an essential component of lipid rafts in cell plasma membrane,which exerts a hepatoprotective role against mycotoxin exposure in pigs,and cholesterol metabolism is vulnerable to epigenetic histone acetylation.Therefore,our present study aimed to investigate whether a histone deacetylase inhibitor(sodium butyrate [NaBu]) could protect the porcine liver from deoxynivalenol(DON) exposure by modulating cholesterol metabolism.Herein,we randomly divided 28 pigs into four groups,which were fed an uncontaminated basal diet,contaminated diet(4 mg DON/kg),uncontaminated diet supplemented with 0.2% NaBu or 4 mg/kg DON contaminated diet(4 mg DON/kg) supplemented with 0.2% NaBu for 28 d.Results:We found that the serum alanine transaminase(ALT),aspartate transaminase(AST),and alkaline phosphatase(ALP) were all increased in pigs exposed to DON,indicative of significant liver injury.Furthermore,the cholesterol content in the serum of DON-exposed pigs was significantly reduced,compared to the healthy Vehicle group.Transcriptome analysis of porcine liver tissues revealed that the cholesterol homeostasis pathway was highly enriched due to DON exposure.In which we validated by qRT-PCR and western blotting that the cholesterol program was markedly activated.Importantly,NaBu effectively restored parameters associated with liver injury,along with the cholesterol content and the expression of key genes involved in the cholesterol biosynthesis pathway.Mechanistically,we performed a ChIP-seq analysis of H3K27ac and showed that NaBu strongly diminished DON-increased H3K27ac genome-wide enrichment.We further validated that the elevated H3K27ac and H3K9ac occupancies on cholesterol biosynthesis genes were both decreased by NaBu,as determined by ChIP-qPCR analysis.Notably,nuclear receptor RORγ,a novel regulator of cholesterol biosynthesis,was found in the hyperacetylated regions.Again,a remarkable increase of RORγ at both mRNA and protein levels in DON-exposed porcine livers was drastically reduced by NaBu.Consistent with RORγ expression,NaBu also hindered RORγ transcriptional binding enrichments on these activated cholesterol biosynthesis genes like HMGCR,SQLE,and DHCR24.Furthermore,we conducted an in vitro luciferase reporter assay to verify that porcine RORγ directly bonds to the promoters of the above target genes.Conclusions:Collectively,our results demonstrate the utility of the natural product Na Bu as a potential anti-mycotoxin nutritional strategy for regulating cholesterol metabolism via RORγ-mediated histone acetylation modification. 展开更多
关键词 Cholesterol biosynthesis DON Histone acetylation RORγ Sodium butyrate
下载PDF
The specificity of ten non-digestible carbohydrates to enhance butyrate-producing bacteria and butyrate production in vitro fermentation
12
作者 Jingjing Xu Ruyue Wang +9 位作者 Weibao Liu Zhongwei Yin Jianrong Wu Xun Yu Wen Wang Hongtao Zhang Zhitao Li Minjie Gao Li Zhu Xiaobei Zhan 《Food Science and Human Wellness》 SCIE CSCD 2023年第6期2344-2354,共11页
Butyrate and butyrate-producing bacteria are important indicators of gut microbial metabolism in human health.Ten non-digestible carbohydrates(NDCs),including inulin,fructooligosaccharide(FOS),oatsβ-glucans(OGS),oats... Butyrate and butyrate-producing bacteria are important indicators of gut microbial metabolism in human health.Ten non-digestible carbohydrates(NDCs),including inulin,fructooligosaccharide(FOS),oatsβ-glucans(OGS),oatsβ-glucan oligosaccharides(OGOS),Astragalus polysaccharides(APS),Astragalus oligosaccharides(AOS),xanthan gum oligosaccharides(XGOS),gellan gum oligosaccharides(GGOS),curdlan oligosaccharides(COS),and pullulan oligosaccharides(POS)were used to investigate NDC specifi city in modulating butyrate-producing bacteria and butyrate production in 48 h in vitro fermentation studies in combination with fecal inocula from 7 healthy donors and 11 patients with type 2 diabetes(T2D).We observed that the amount of these ten NDCs utilized depended on NDC structure and inter-individual gut microbial differences.XGOS and GGOS fermentations signifi cantly increased butyrate-producing bacteria(especially f_Lachnospiraceae)and butyric acid production.Furthermore,XGOS and GGOS fermentations showed a better ability to consistently modulate gut microbiota composition and metabolic properties between individuals of healthy donors or T2D patients when compared to inulin,FOS,APS,AOS,OGS,OGOS,COS and POS fermentation.This research indicated that xanthan gum and gellan gum oligosaccharides have strong specifi city to enhance butyrate-producing bacteria and butyrate production. 展开更多
关键词 Non-digestible carbohydrates Gut microbiota butyric acid butyrate-producing bacteria
下载PDF
丁酸钠通过G蛋白偶联受体41介导蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路刺激牛乳腺上皮细胞增殖 被引量:1
13
作者 张静 卜丽君 +4 位作者 郎姣姣 刘亚鹏 夏呈强 霍文婕 刘强 《动物营养学报》 CAS CSCD 北大核心 2024年第3期1878-1891,共14页
本试验旨在研究丁酸钠(SB)刺激牛乳腺上皮细胞(BMECs)增殖的分子机制。采用单因素设计,以含不同浓度(0、15、30、45、60和75μmol/L)SB的DMEM/F12培养基(含10%新生胎牛血清)培养BMECs,通过细胞计数试剂盒(CCK⁃8)检测细胞活性,确定适宜S... 本试验旨在研究丁酸钠(SB)刺激牛乳腺上皮细胞(BMECs)增殖的分子机制。采用单因素设计,以含不同浓度(0、15、30、45、60和75μmol/L)SB的DMEM/F12培养基(含10%新生胎牛血清)培养BMECs,通过细胞计数试剂盒(CCK⁃8)检测细胞活性,确定适宜SB浓度。然后以对照(0μmol/L)和适宜SB浓度培养BMECs,并采用蛋白激酶B(Akt)阻断剂(AKT⁃IN⁃1)、哺乳动物雷帕霉素靶蛋白(mTOR)阻断剂雷帕霉素(Rap)或小干扰RNA(siR⁃NA)沉默G蛋白偶联受体41(GPR41)对信号通路及受体进行处理,检测BMECs细胞增殖、凋亡以及GPR41和Akt/mTOR信号通路相关基因和蛋白表达的变化。结果表明:1)与对照组相比,60μmol/L的SB显著提高了BMECs细胞活力(P<0.05),75μmol/L的SB显著抑制了BMECs细胞活力(P<0.05);60μmol/L的SB极显著增加了增殖细胞核抗原(PCNA)、细胞周期蛋白A2(CCNA2)和细胞周期蛋白D1(CCND1)的mRNA相对表达量(P<0.01),显著增加了PCNA和细胞周期蛋白A1(CCNA1)的蛋白相对表达量(P<0.05)。2)与对照组相比,60μmol/L SB显著或极显著提高了B细胞淋巴瘤2(BCL2)的mRNA和蛋白相对表达量及BCL2/B细胞淋巴瘤2相关X蛋白(BAX)比值(P<0.05或P<0.01),显著或极显著降低了BAX、半胱氨酸天冬氨酸蛋白酶-3(Caspase⁃3)和半胱氨酸天冬氨酸蛋白酶-9(Caspase⁃9)的mRNA和蛋白相对表达量(P<0.05),同时显著或极显著提高了磷酸化蛋白激酶B(p⁃Akt)/Akt和磷酸化哺乳动物雷帕霉素靶蛋白(p⁃mTOR)/mTOR比值(P<0.05或P<0.01)。60μmol/L的SB对Akt和mTOR信号通路的激活被AKT⁃IN⁃1极显著阻断(P<0.01),而Rap抑制mTOR完全逆转了60μmol/L SB对BMECs增殖的促进作用以及增殖基因和蛋白表达的改变(P<0.05或P<0.01),但不影响与凋亡和SB激活的Akt信号通路相关的mRNA或蛋白表达(P>0.05)。3)与对照组相比,60μmol/L的SB极显著提高了GPR41的mRNA相对表达量(P<0.01),显著提高了GPR41蛋白相对表达量(P<0.05)。GPR41 siRNA沉默GPR41后完全逆转了60μmol/L SB对BMECs增殖的促进作用以及Akt/mTOR信号通路相关的增殖基因和蛋白的mRNA或蛋白表达(P<0.05或P<0.01)。由此可见,60μmol/L SB可通过GPR41介导Akt/mTOR信号通路促进BMECs增殖。 展开更多
关键词 Akt mTOR 信号通路 牛乳腺上皮细胞 增殖 丁酸钠
下载PDF
3种不同添加物对凡纳滨对虾生长、非特异免疫和抗病力的影响 被引量:1
14
作者 田相利 秦光彩 +6 位作者 罗凯 汪仕爽 刘杨 魏聪 王明阳 董双林 刘清兵 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期33-43,共11页
为评估3种不同添加物对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异免疫反应及抗病力的影响,在基础饲料中分别添加了丁酸梭菌CBG01(Clostridium butyricum CBG01)活菌(CB组)、3%聚β-羟基丁酸酯(PHB组)和1%丁酸钠(BS组)来投喂对... 为评估3种不同添加物对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异免疫反应及抗病力的影响,在基础饲料中分别添加了丁酸梭菌CBG01(Clostridium butyricum CBG01)活菌(CB组)、3%聚β-羟基丁酸酯(PHB组)和1%丁酸钠(BS组)来投喂对虾,对照组投喂基础饲料,养殖6周后测量对虾生长情况、检测血清非特异免疫指标和肝胰腺免疫相关基因表达水平,进行副溶血弧菌(Vibrio parahaemolyticus)攻毒实验。研究表明,CB组和PHB组对虾末体质量和特定生长率显著高于对照组(P<0.05),CB组最高,BS组与对照组差异不显著(P>0.05)。3个处理组对虾成活率和饲料效率均显著高于对照组(P<0.05)。3个处理组对虾血清中溶菌酶、过氧化物酶、超氧化物歧化酶活性以及总抗氧化能力显著高于对照组(P<0.05),CB组对虾血清中碱性磷酸酶、酸性磷酸酶、酚氧化酶活性显著高于对照组(P<0.05),PHB组对虾血清中碱性磷酸酶、酸性磷酸酶、总一氧化氮合酶活性显著高于对照组(P<0.05),BS组对虾血清中酸性磷酸酶活性显著高于对照组(P<0.05)。与对照组相比,CB组对虾肝胰腺中所有免疫相关基因(SOD、LZM、proPO、LGBP、HSP70、Imd、Toll、Relish、TOR、4E-BP、eIF4E1α、eIF4E2)相对表达量均显著上调(P<0.05),而PHB组的Toll基因以及BS组的Imd、Toll、Relish、eIF4E2基因相对表达量与对照组无显著差异(P>0.05)。副溶血弧菌攻毒实验表明,3个处理组对虾的累积死亡率均显著低于对照组(P<0.05)。研究结果表明,饲料中添加丁酸梭菌、PHB和丁酸钠均可不同程度地提高凡纳滨对虾的生长性能和非特异免疫能力,能够显著提高对虾对副溶血弧菌感染的抵抗力。总体比较,添加丁酸梭菌和PHB的作用效果基本相当,在一定程度上要优于添加丁酸钠。在饲料生产加工过程中,在难以添加丁酸梭菌活菌的情况下,建议适当添加聚β-羟基丁酸酯来替代丁酸梭菌。 展开更多
关键词 丁酸梭菌 聚Β-羟基丁酸酯 丁酸钠 凡纳滨对虾 生长 非特异性免疫 抗病力 副溶血弧菌
下载PDF
紫外线屏蔽PDA/BC/PVB复合透明薄膜的制备及性能
15
作者 晋玉霞 刘永超 +2 位作者 任小逆 刘恒源 潘玮 《工程塑料应用》 CAS CSCD 北大核心 2024年第8期52-57,共6页
聚乙烯醇缩丁醛(PVB)薄膜制品在包装材料领域得到了广泛应用,然而PVB材料的光降解导致材料的应用价值降低,因此,设计防紫外线PVB复合材料被认为是实现其应用价值的必要条件。以盐酸多巴胺(DA)、细菌纤维素(BC)和PVB为原材料,首先基于DA... 聚乙烯醇缩丁醛(PVB)薄膜制品在包装材料领域得到了广泛应用,然而PVB材料的光降解导致材料的应用价值降低,因此,设计防紫外线PVB复合材料被认为是实现其应用价值的必要条件。以盐酸多巴胺(DA)、细菌纤维素(BC)和PVB为原材料,首先基于DA的氧化自聚合,在BC表面原位沉积聚多巴胺(PDA)对BC改性,得到PDA/BC纳米复合材料,然后通过溶液共混和涂膜工艺将PDA/BC与PVB共混,制备了PDA/BC/PVB复合纳米薄膜,并使用扫描电子显微镜(SEM)、紫外-可见红外分光光度计、万能材料试验机、傅里叶变换红外光谱以及X射线衍射仪研究了PDA/BC的添加量对PDA/BC/PVB复合薄膜结构与性能的影响。SEM表明,当DA的质量分数为0.1%时,反应得到的PDA可均匀覆盖在BC的表面,且制备的PDA/BC/PVB复合薄膜的截面致密;当PDA/BC质量分数小于1%时PDA/BC在PVB中分散均匀。紫外透过率结果表明,当加入质量分数为1%的PDA/BC时,PDA/BC/PVB复合材料在370 nm处紫外屏蔽效率达到98%,复合透明薄膜表现出优异的紫外线阻隔性能,材料的拉伸强度高达61.2 MPa,远高于原始PVB的强度(39.7 MPa)。光学照片显示PDA/BC的加入并未改变材料的透明性。 展开更多
关键词 聚乙烯醇缩丁醛 原位聚合 聚多巴胺 紫外线屏蔽 纤维增强
下载PDF
丁酸盐在炎症性肠病中的免疫调节机制研究进展
16
作者 崔馨月 石璠 +1 位作者 郑丽红 王海强 《河北医药》 CAS 2024年第9期1397-1402,共6页
炎症性肠病(IBD)是一组与肠道慢性炎症相关的异质性疾病,丁酸盐是肠道微生物群产生的关键代谢产物,能够调节免疫细胞的发育和功能,调节免疫功能并防止过度免疫反应,从而延缓IBD的临床进展。本文就丁酸盐在调节免疫功能方面改善IBD作用... 炎症性肠病(IBD)是一组与肠道慢性炎症相关的异质性疾病,丁酸盐是肠道微生物群产生的关键代谢产物,能够调节免疫细胞的发育和功能,调节免疫功能并防止过度免疫反应,从而延缓IBD的临床进展。本文就丁酸盐在调节免疫功能方面改善IBD作用机制的研究进展进行综述,旨在为IBD的临床治疗提供新的选择。 展开更多
关键词 炎症性肠病 丁酸盐 G蛋白耦联受体 TH17细胞 TREG细胞
下载PDF
蛋白质琥珀酰化参与丁酸抑制胃癌细胞增殖及迁移的作用
17
作者 黄颖鹏 张珂 +3 位作者 吴芳全 施迪邦 韩千年 王方岩 《温州医科大学学报》 CAS 2024年第2期99-105,共7页
目的:研究丁酸对胃癌细胞增殖及代谢的影响,并探讨其潜在作用机制。方法:使用5 mmol/L丁酸钠处理胃癌细胞48 h后,通过EdU染色及Transwell法检测细胞增殖、迁移情况;收集丁酸处理后的胃癌细胞,提取代谢物进行代谢组学分析筛选出其中显著... 目的:研究丁酸对胃癌细胞增殖及代谢的影响,并探讨其潜在作用机制。方法:使用5 mmol/L丁酸钠处理胃癌细胞48 h后,通过EdU染色及Transwell法检测细胞增殖、迁移情况;收集丁酸处理后的胃癌细胞,提取代谢物进行代谢组学分析筛选出其中显著富集的代谢通路以及上调的代谢物;采用Western blot以及细胞免疫荧光检测丁酸钠处理后胃癌细胞蛋白质琥珀酰化程度,同时采用RT-qPCR检测SIRT5、SUCLG1、SUCLG2和SUCLA2的mRNA水平。结果:丁酸钠处理48 h后胃癌细胞增殖被显著抑制,细胞中检测到三羧酸循环及氧化磷酸化等代谢通路显著富集,其中三羧酸循环代谢物L-苹果酸、琥珀酰辅酶A及延胡索酸显著上调(均P<0.05);Western blot和免疫荧光显示丁酸处理后胃癌细胞蛋白琥珀酰化水平上调(P<0.05)。同时RT-qPCR证实了丁酸处理上调了琥珀酰辅酶A合成酶编码基因SUCLG1、SUCLG2和SUCLA2的mRNA表达水平(均P<0.05),但并未影响SIRT5表达(P>0.05)。结论:丁酸能够抑制胃癌细胞增殖及迁移,其可能通过促进SUCLs表达以及上调琥珀酰辅酶A水平并进一步促进胃癌细胞蛋白质琥珀酰化实现。 展开更多
关键词 丁酸 胃肿瘤 代谢组学 琥珀酰化 琥珀酰辅酶A
下载PDF
0.01%28-高芸苔素内酯·吲哚丁酸对寒地水稻生长及产量的影响
18
作者 黄成亮 《北方水稻》 CAS 2024年第1期30-34,42,共6页
为明确0.01%28-高芸苔素内酯·吲哚丁酸对寒地水稻生长调节作用,采用随机区组试验设计,研究不同施药量对寒地水稻生长及产量的影响。结果表明,于分蘖期施用0.01%28-高芸苔素内酯·吲哚丁酸颗粒剂,能明显促进水稻分蘖,缩短始穗... 为明确0.01%28-高芸苔素内酯·吲哚丁酸对寒地水稻生长调节作用,采用随机区组试验设计,研究不同施药量对寒地水稻生长及产量的影响。结果表明,于分蘖期施用0.01%28-高芸苔素内酯·吲哚丁酸颗粒剂,能明显促进水稻分蘖,缩短始穗期至齐穗期天数,促进干物质积累,增强抗倒伏能力,提高水稻产量,对水稻生长安全。药剂施用量为22.5 kg/hm^(2)时,水稻穗粒数、结实率、千粒重最大,产量最高,是最佳施用量。 展开更多
关键词 0.01%28-高芸苔素内酯·吲哚丁酸 寒地水稻 生长 产量
下载PDF
制粒丁酸钠对蛋鸭产蛋性能、肠道组织形态及菌群的影响 被引量:1
19
作者 李成凤 黄曼曼 +5 位作者 孙寒雪 屈元启 李浙烽 曾涛 田勇 卢立志 《中国家禽》 北大核心 2024年第4期31-39,共9页
试验旨在研究制粒丁酸钠对蛋鸭产蛋性能、肠道组织形态及菌群的影响。试验选用120只48周龄体重与产蛋率相近的蛋鸭分为2组:对照组(C组)饲喂基础日粮,制粒丁酸钠组(GSB组)在基础日粮中添加500 mg/kg制粒丁酸钠。每组6个重复,每个重复10只... 试验旨在研究制粒丁酸钠对蛋鸭产蛋性能、肠道组织形态及菌群的影响。试验选用120只48周龄体重与产蛋率相近的蛋鸭分为2组:对照组(C组)饲喂基础日粮,制粒丁酸钠组(GSB组)在基础日粮中添加500 mg/kg制粒丁酸钠。每组6个重复,每个重复10只鸭,试验期60 d。结果显示:制粒丁酸钠组蛋鸭产蛋率、平均日采食量、蛋重和料蛋比与对照组差异不显著(P>0.05),与对照组相比,制粒丁酸钠组蛋鸭十二指肠绒毛高度显著增加(P<0.05),隐窝深度显著减少(P<0.05),绒毛高度与隐窝深度之比显著增加(P<0.01)。盲肠菌群多样性结果显示,制粒丁酸钠组蛋鸭盲肠菌群的Chao1指数显著高于对照组(P<0.05);制粒丁酸钠组蛋鸭盲肠菌群的Good's coverage指数显著低于对照组(P<0.05)。与对照组相比,制粒丁酸钠组蛋鸭盲肠拟杆菌门丰度显著降低(P<0.05),变形菌门和放线菌门丰度显著提高(P<0.01),制粒丁酸钠组蛋鸭盲肠普雷沃菌属显著降低(P<0.01),栖粪杆菌属丰度显著提高(P<0.05)。通过LEfSe分析发现对照组中主要的差异菌群为拟杆菌门、拟杆菌纲、拟杆菌目、拟杆菌属和拟杆菌科(P<0.05),制粒丁酸钠组中主要的差异菌群为变形菌门、放线菌门和栖粪杆菌属等(P<0.05)。综上所述,饲粮添加500 mg/kg制粒丁酸钠可以改善蛋鸭肠道组织形态,提高蛋鸭盲肠菌群丰富度,增加有益菌群丰度并减少致病菌的定植,从而促进蛋鸭肠道健康。 展开更多
关键词 制粒丁酸钠 蛋鸭 肠道组织形态 肠道菌群
原文传递
Effects of β-Amino Butyric Acid Induced Rice Blast Resistance on Reactive Oxygen Metabolism 被引量:5
20
作者 李莉 郭晓丽 +5 位作者 刘晓梅 温嘉伟 张秀容 孙辉 赵宇 任金平 《Agricultural Science & Technology》 CAS 2009年第3期112-114,共3页
[Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly sus... [Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly susceptible to disease as experimental material, the changes of catalase(CAT), and superoxide dismutase(SOD) and MDA activities in rice treated by BABA were investigated. [Result] In rice plants treated by BABA, the activities of CAT and SOD increased, meanwhile the MDA content also rose to some extent, resulting in the disease resistance to rice blast. [Conclusion] By influencing reactive oxygen metabolism, BABA endows rice plants with resistance to rice blast. BABA is safe to environment and has highly resistance-inducing capacity, it could be generalized in production. 展开更多
关键词 β-Amino butyric acid Rice blast Induced resistance
下载PDF
上一页 1 2 103 下一页 到第
使用帮助 返回顶部