The prevalence of antibiotic susceptibility and disinfectant resistance in bacterial pathogens causes a serious problem not only to food safety but also to public health, which directly or indirectly leads to treatmen...The prevalence of antibiotic susceptibility and disinfectant resistance in bacterial pathogens causes a serious problem not only to food safety but also to public health, which directly or indirectly leads to treatment and disinfection failures. In this review, multidrug resistance, the mechanism of disinfectant resistance, the methods for detecting disinfectant resistance and the cross-resistance between an- tibiotics and disinfectants are summarized. More efforts should be devoted to explor- ing the professional guidance of using antibiotics and disinfectants, and to develop- ing the comprehensive detection with genetic and molecular methods is highly ex- pected.展开更多
In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with ...In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with a broad spectrum of antimicrobial effect.It is vital to inhibit the spread of pathogen in hospital.However,a large number of pathogens with the decreased antiseptic susceptibility have been isolated from clinical samples which showed an increased minimal inhibitory concentration(MIC)against those antiseptics.These resistant pathogens are the major causes for nosocomial crossinfections in hospital.The present study demonstrated the utility of Oxford plate assay system in determining the potential disinfectant resistance of bacteria.The microbiological assay is based on the inhibitory effect of tested disinfectants upon the strains of Staphylococcus aureus and Escherichia coli.Statistical analysis of the bioassay results indicated the linear correlation(r=-0.87-0.99,P<0.01)between the diameter of growth inhibition zone and the log dosage of the tested disinfectants.Moreover,comparison of inhibitory efficacy of benzalkonium bromide upon 29 S.aureus strains isolated from clinical samples by both Oxford plate method and broth dilution method showed that the diameter of growth inhibition zone has significantly negative correlation with the minimal inhibitory concentration(MIC)(r=-0.574,P<0.001).These results suggest that the Oxford plate is a simple and time-saving method in detecting potential clinical disinfectant resistance and its usefulness for routine surveillance of pathogenic resistance to disinfectants warrants further investigation.展开更多
This study compared three different disinfection processes （chlorination, E-beam, and ozone） and the efficacy of three oxidants （H202, S2O8-, and peroxymonosulfate （MPS）） in removing antibiotic resistant bacter...This study compared three different disinfection processes （chlorination, E-beam, and ozone） and the efficacy of three oxidants （H202, S2O8-, and peroxymonosulfate （MPS）） in removing antibiotic resistant bacteria （ARB） and antibiotic resistance genes （ARGs） in a synthetic wastewater. More than 30 mg/L of chlorine was needed to remove over 90% of ARB and ARG. For the E-beam method, only 1 dose （kGy） was needed to remove ARB and ARG, and ozone could reduce ARB and ARG by more than 90% even at 3 mg/L ozone concentration. In the ozone process, CT values （concentration × time） were compared for ozone alone and combined with different catalysts based on the 2-log removal of ARB and ARG. Ozone treatment yielded a value of 31 and 33 （mg·min）/L for ARB and ARGs respectively. On the other hand, ozone with persulfate yielded 15.9 and 18.5 （mg.min）/L while ozone with monopersulfate yielded a value of 12 and 14.5 （mg·min）/L. This implies that the addition of these catalysts significantly reduces the contact time to achieve a 2-log removal, thus enhancing the process in terms of its kinetics.展开更多
基金Supported by the National Key Research and Development Program of China(2016YFD0501208)the Social Development Program of Yangzhou(YZ2016058)+1 种基金the National Major Project for Agro-product Quality&Safety Risk Assessment(GJFP2017007)the Project for the Construction of Science and Technology Service Platform for Poultry Quality and Safety of Yangzhou(yz2015162)~~
文摘The prevalence of antibiotic susceptibility and disinfectant resistance in bacterial pathogens causes a serious problem not only to food safety but also to public health, which directly or indirectly leads to treatment and disinfection failures. In this review, multidrug resistance, the mechanism of disinfectant resistance, the methods for detecting disinfectant resistance and the cross-resistance between an- tibiotics and disinfectants are summarized. More efforts should be devoted to explor- ing the professional guidance of using antibiotics and disinfectants, and to develop- ing the comprehensive detection with genetic and molecular methods is highly ex- pected.
文摘In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with a broad spectrum of antimicrobial effect.It is vital to inhibit the spread of pathogen in hospital.However,a large number of pathogens with the decreased antiseptic susceptibility have been isolated from clinical samples which showed an increased minimal inhibitory concentration(MIC)against those antiseptics.These resistant pathogens are the major causes for nosocomial crossinfections in hospital.The present study demonstrated the utility of Oxford plate assay system in determining the potential disinfectant resistance of bacteria.The microbiological assay is based on the inhibitory effect of tested disinfectants upon the strains of Staphylococcus aureus and Escherichia coli.Statistical analysis of the bioassay results indicated the linear correlation(r=-0.87-0.99,P<0.01)between the diameter of growth inhibition zone and the log dosage of the tested disinfectants.Moreover,comparison of inhibitory efficacy of benzalkonium bromide upon 29 S.aureus strains isolated from clinical samples by both Oxford plate method and broth dilution method showed that the diameter of growth inhibition zone has significantly negative correlation with the minimal inhibitory concentration(MIC)(r=-0.574,P<0.001).These results suggest that the Oxford plate is a simple and time-saving method in detecting potential clinical disinfectant resistance and its usefulness for routine surveillance of pathogenic resistance to disinfectants warrants further investigation.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (No. 2012-0003505)Korea Ministry of Environment as "Global Top Project" (No. GT-11-B-01-005-1)
文摘This study compared three different disinfection processes （chlorination, E-beam, and ozone） and the efficacy of three oxidants （H202, S2O8-, and peroxymonosulfate （MPS）） in removing antibiotic resistant bacteria （ARB） and antibiotic resistance genes （ARGs） in a synthetic wastewater. More than 30 mg/L of chlorine was needed to remove over 90% of ARB and ARG. For the E-beam method, only 1 dose （kGy） was needed to remove ARB and ARG, and ozone could reduce ARB and ARG by more than 90% even at 3 mg/L ozone concentration. In the ozone process, CT values （concentration × time） were compared for ozone alone and combined with different catalysts based on the 2-log removal of ARB and ARG. Ozone treatment yielded a value of 31 and 33 （mg·min）/L for ARB and ARGs respectively. On the other hand, ozone with persulfate yielded 15.9 and 18.5 （mg.min）/L while ozone with monopersulfate yielded a value of 12 and 14.5 （mg·min）/L. This implies that the addition of these catalysts significantly reduces the contact time to achieve a 2-log removal, thus enhancing the process in terms of its kinetics.
