Nuclear safety is a global growing concern,where ionizing radiation(IR)is a major injury factor resulting in serious damage to organisms.The detection of IR is usually conducted with physical dosimeters;however,biolog...Nuclear safety is a global growing concern,where ionizing radiation(IR)is a major injury factor resulting in serious damage to organisms.The detection of IR is usually conducted with physical dosimeters;however,biological IR detection methods are deficient.Here,a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed for the biological detection of IR.Three strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were constructed with IR-dependent fluorescence,and the DH5αstrain was finally selected due to its highest radiation response and fluorescence.Engineered bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different rheology,3D printability and bacterial applicability.A high-gelatin-content hydrogel containing 10%gelatin/1.25%sodium alginatewas optimal.The optimal living composite hydrogelwas 3D-printedwith the special bioink,which reported significant green fluorescence underγ-ray radiation.The living composite hydrogel provides a biological strategy for the detection of environmental ionizing radiation.展开更多
The development of drug delivery vehicles is in significant demand in the context of precision medicine.With the development of synthetic biology,the use of genetically engineered bacteria as drug delivery vectors has...The development of drug delivery vehicles is in significant demand in the context of precision medicine.With the development of synthetic biology,the use of genetically engineered bacteria as drug delivery vectors has attracted more and more attention.Herein,we reviewed the research advances in bioengineered bacteria as drug carriers,with emphasis on the synthetic biology strategies for modifying these bacteria,including the targeted realization method of engineered bacteria,the designing scheme of genetic circuits,and the release pathways of therapeutic compounds.Based on this,the essential components,design principles,and health concerns of engineering bacteria as drug carriers and the development prospects in this field have been discussed.展开更多
Bacteria-mediated anti-tumor therapy has received widespread attention due to its natural tumor-targeting ability and specific immune-activation characteristics.It has made significant progress in breaking the limitat...Bacteria-mediated anti-tumor therapy has received widespread attention due to its natural tumor-targeting ability and specific immune-activation characteristics.It has made significant progress in breaking the limitations of monotherapy and effectively eradicating tumors,especially when combined with traditional therapy,such as radiotherapy.According to their different biological characteristics,bacteria and their derivatives can not only improve the sensitivity of tumor radiotherapy but also protect normal tissues.Moreover,genetically engineered bacteria and bacteria-based biomaterials have further expanded the scope of their applications in radiotherapy.In this review,we have summarized relevant researches on the application of bacteria and its derivatives in radiotherapy in recent years,expounding that the bacteria,bacterial derivatives and bacteria-based biomaterials can not only directly enhance radiotherapy but also improve the anti-tumor effect by improving the tumor microenvironment(TME)and immune effects.Furthermore,some probiotics can also protect normal tissues and organs such as intestines from radiation via anti-inflammatory,anti-oxidation and apoptosis inhibition.In conclusion,the prospect of bacteria in radiotherapy will be very extensive,but its biological safety and mechanism need to be further evaluated and studied.展开更多
Conventional photodynamic therapy(PDT)approaches face challenges including limited light penetration,low uptake of photosensitizers by tumors,and lack of oxygen in tumor microenvironments.One promising solution is to ...Conventional photodynamic therapy(PDT)approaches face challenges including limited light penetration,low uptake of photosensitizers by tumors,and lack of oxygen in tumor microenvironments.One promising solution is to internally generate light,photosensitizers,and oxygen.This can be accomplished through endogenous production,such as using bioluminescence as an endogenous light source,synthesizing genetically encodable photosensitizers in situ,and modifying cells genetically to express catalase enzymes.Furthermore,these strategies have been reinforced by the recent rapid advancements in synthetic biology.In this review,we summarize and discuss the approaches to overcome PDT obstacles by means of endogenous production of excitation light,photosensitizers,and oxygen.We envision that as synthetic biology advances,genetically engineered cells could act as precise and targeted“living factories”to produce PDT components,leading to enhanced performance of PDT.展开更多
基金supported by the Special Program for Capability Promotion
文摘Nuclear safety is a global growing concern,where ionizing radiation(IR)is a major injury factor resulting in serious damage to organisms.The detection of IR is usually conducted with physical dosimeters;however,biological IR detection methods are deficient.Here,a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed for the biological detection of IR.Three strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were constructed with IR-dependent fluorescence,and the DH5αstrain was finally selected due to its highest radiation response and fluorescence.Engineered bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different rheology,3D printability and bacterial applicability.A high-gelatin-content hydrogel containing 10%gelatin/1.25%sodium alginatewas optimal.The optimal living composite hydrogelwas 3D-printedwith the special bioink,which reported significant green fluorescence underγ-ray radiation.The living composite hydrogel provides a biological strategy for the detection of environmental ionizing radiation.
基金This work is supported by I-Corps at Ohio(No.UT22117).
文摘The development of drug delivery vehicles is in significant demand in the context of precision medicine.With the development of synthetic biology,the use of genetically engineered bacteria as drug delivery vectors has attracted more and more attention.Herein,we reviewed the research advances in bioengineered bacteria as drug carriers,with emphasis on the synthetic biology strategies for modifying these bacteria,including the targeted realization method of engineered bacteria,the designing scheme of genetic circuits,and the release pathways of therapeutic compounds.Based on this,the essential components,design principles,and health concerns of engineering bacteria as drug carriers and the development prospects in this field have been discussed.
基金partially supported by the National Natural Science Foundation of China(U1932208,32171382)Key Research and Development Program of Social Development of Jiangsu Province(BE2022725,China)+2 种基金Hui-Chun Chin and Tsung-Dao Lee Chinese Undergraduate Research Endowment(CURE,China)Undergraduate Training Program for Innovation and Entrepreneurship Soochow University(202010285046Z,China)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,China)。
文摘Bacteria-mediated anti-tumor therapy has received widespread attention due to its natural tumor-targeting ability and specific immune-activation characteristics.It has made significant progress in breaking the limitations of monotherapy and effectively eradicating tumors,especially when combined with traditional therapy,such as radiotherapy.According to their different biological characteristics,bacteria and their derivatives can not only improve the sensitivity of tumor radiotherapy but also protect normal tissues.Moreover,genetically engineered bacteria and bacteria-based biomaterials have further expanded the scope of their applications in radiotherapy.In this review,we have summarized relevant researches on the application of bacteria and its derivatives in radiotherapy in recent years,expounding that the bacteria,bacterial derivatives and bacteria-based biomaterials can not only directly enhance radiotherapy but also improve the anti-tumor effect by improving the tumor microenvironment(TME)and immune effects.Furthermore,some probiotics can also protect normal tissues and organs such as intestines from radiation via anti-inflammatory,anti-oxidation and apoptosis inhibition.In conclusion,the prospect of bacteria in radiotherapy will be very extensive,but its biological safety and mechanism need to be further evaluated and studied.
基金supported by the National Natural Science Foundation of China(32000036)。
文摘Conventional photodynamic therapy(PDT)approaches face challenges including limited light penetration,low uptake of photosensitizers by tumors,and lack of oxygen in tumor microenvironments.One promising solution is to internally generate light,photosensitizers,and oxygen.This can be accomplished through endogenous production,such as using bioluminescence as an endogenous light source,synthesizing genetically encodable photosensitizers in situ,and modifying cells genetically to express catalase enzymes.Furthermore,these strategies have been reinforced by the recent rapid advancements in synthetic biology.In this review,we summarize and discuss the approaches to overcome PDT obstacles by means of endogenous production of excitation light,photosensitizers,and oxygen.We envision that as synthetic biology advances,genetically engineered cells could act as precise and targeted“living factories”to produce PDT components,leading to enhanced performance of PDT.
