Direct sewage discharge may enhance soil nitrous oxide(N_(2)O)emissions,worsening the greenhouse effect.However,the effects of sewage discharge into bogs on N_(2)O flux,drivers and influencing mechanisms remain unclea...Direct sewage discharge may enhance soil nitrous oxide(N_(2)O)emissions,worsening the greenhouse effect.However,the effects of sewage discharge into bogs on N_(2)O flux,drivers and influencing mechanisms remain unclear.Additionally,investigating the impact of reclaimed water on N_(2)O flux is important for bog replenishment and water shortage alleviation.This study simulated sewage from different sources into a bog and analyzed N_(2)O fluxes,soil(organic carbon,total nitrogen,ammonium nitrogen,nitrate nitrogen,total phosphorus,available phosphorus,pH and electrical conductivity),plant(species richness and biomass)and microorganisms(ammonia-oxidizing archaea,napA,nirS,nirK and nosZ genes).Results showed that the reclaimed water did not significantly change N_(2)O flux,while 50%tap water mixed with 50%domestic sewage and domestic sewage significantly increased the N_(2)O flux.Among soil factors,available nitrogen and pH were key in influencing N_(2)O flux.Among plant parameters,species richness was the primary factor affecting N_(2)O flux.Nitrogen transformation functional genes contributed the most to the increase in the N_(2)O fluxes,with an increase in domestic sewage input leading to a higher abundance of these genes and subsequent N_(2)O emissions.Therefore,domestic sewage should be considered,as it significantly increases N_(2)O emissions by affecting the soil,plants and microorganisms,thereby increasing the global warming potential.This study’s findings suggest that using treated reclaimed water for bog replenishment could be an environmentally friendly approach to wetland management.展开更多
Soil metaproteomics has excellent potential as a tool to elucidate the structural and functional changes in soil microbial communities in response to environmental alterations. However, soil metaproteomics is hindered...Soil metaproteomics has excellent potential as a tool to elucidate the structural and functional changes in soil microbial communities in response to environmental alterations. However, soil metaproteomics is hindered by several challenges and gaps. Soil microbial communities possess extremely complex microbial composition, including many uncultured microorganisms without whole genome sequencing. Thus, how to select a suitable protein sequence database remains challenging in soil metaproteomics. In this study, the Public database and Meta-database were constructed using protein sequences from public databases and metagenomics, respectively. We comprehensively analyzed and compared the soil metaproteomic results using these two kinds of protein sequence databases for protein identification based on published soil metaproteomic raw data. The results demonstrated that many more proteins, higher sequence coverage, and even more microbial species and functional annotations could be identified using the Meta-database compared with those identified using the Public database. These findings indicated that the Meta-database was more specific as a protein sequence database. However, the follow-up in-depth metaproteomic analyses exhibited similar main results regardless of the database used. The microbial community composition at the genus level was similar between the two databases, especially the species annotations with high peptide-spectrum match and high abundance. The functional analyses in response to stress, such as the gene ontology enrichment of biological progress and molecular function and the key functional microorganisms, were also similar regardless of the database. Our analysis revealed that the Public database could also meet the demand to explore the functional responses of microbial proteins to some extent. This study provides valuable insights into the choice of protein sequence databases and their impacts on subsequent bioinformatic analysis in soil metaproteomic research and will facilitate the optimization of experimental design for different purposes.展开更多
Surfactants were expected to exhibit positive effects on the waste activated sludge(WAS)disposal.However,the systematic comparison of different categories of surfactants on the WAS fermentation and the functional mech...Surfactants were expected to exhibit positive effects on the waste activated sludge(WAS)disposal.However,the systematic comparison of different categories of surfactants on the WAS fermentation and the functional mechanisms,especially microbial metabolic traits,have not yet been precisely explored.This study revealed the positive effects of different surfactants on the volatile fatty acid(VFA)production,which followed the order of alkyl polysaccharides(APG)>sodium dodecylbenzene sulfonate(SDBS)>hexadecyl trimethyl ammonium bromide(HTAB).Mechanistic exploration found that the presence of different surfactants improved solubilization and hydrolysis steps,and then contributed to the subsequent acidification with different efficiencies.The functional microorganisms associated with VFA generation were enriched in surfactant-conditioned reactors.Metagenomic analysis further indicated that the key genes involved in the particular process of VFA generation were over-expressed.The simultaneous bioavailable substrate improvement,functional bacterial enrichment,and metabolic activity upregulation induced by different surfactants jointly contributed to VFA promotion during WAS fermentation.This study could provide a comprehensive realization of surfactants’impacts on theWAS fermentation process,and more importantly,it reminded the public to discern the distinct interplaying effects induced by different chemicals in regulating the WAS disposal and resource recovery.展开更多
基金supported by the National Key R&D Program of China(2022YFF1300900)the National Natural Science Foundation of China(32271624)+1 种基金the Foundation of Jilin Scientifc and Technological Development Project(20220203003SF)the Education Department of Jilin Province(JJKH20230516KJ).
文摘Direct sewage discharge may enhance soil nitrous oxide(N_(2)O)emissions,worsening the greenhouse effect.However,the effects of sewage discharge into bogs on N_(2)O flux,drivers and influencing mechanisms remain unclear.Additionally,investigating the impact of reclaimed water on N_(2)O flux is important for bog replenishment and water shortage alleviation.This study simulated sewage from different sources into a bog and analyzed N_(2)O fluxes,soil(organic carbon,total nitrogen,ammonium nitrogen,nitrate nitrogen,total phosphorus,available phosphorus,pH and electrical conductivity),plant(species richness and biomass)and microorganisms(ammonia-oxidizing archaea,napA,nirS,nirK and nosZ genes).Results showed that the reclaimed water did not significantly change N_(2)O flux,while 50%tap water mixed with 50%domestic sewage and domestic sewage significantly increased the N_(2)O flux.Among soil factors,available nitrogen and pH were key in influencing N_(2)O flux.Among plant parameters,species richness was the primary factor affecting N_(2)O flux.Nitrogen transformation functional genes contributed the most to the increase in the N_(2)O fluxes,with an increase in domestic sewage input leading to a higher abundance of these genes and subsequent N_(2)O emissions.Therefore,domestic sewage should be considered,as it significantly increases N_(2)O emissions by affecting the soil,plants and microorganisms,thereby increasing the global warming potential.This study’s findings suggest that using treated reclaimed water for bog replenishment could be an environmentally friendly approach to wetland management.
基金supported by the National Key Research and Development Program of China(No.2016YFD0200-308)the National Key Basic Research Program of China(No.2015CB150501)the Project of Priority and Key Areas,Institute of Soil Science,Chinese Academy of Sciences(Nos.ISSASIP1605 and ISSASIP1640).
文摘Soil metaproteomics has excellent potential as a tool to elucidate the structural and functional changes in soil microbial communities in response to environmental alterations. However, soil metaproteomics is hindered by several challenges and gaps. Soil microbial communities possess extremely complex microbial composition, including many uncultured microorganisms without whole genome sequencing. Thus, how to select a suitable protein sequence database remains challenging in soil metaproteomics. In this study, the Public database and Meta-database were constructed using protein sequences from public databases and metagenomics, respectively. We comprehensively analyzed and compared the soil metaproteomic results using these two kinds of protein sequence databases for protein identification based on published soil metaproteomic raw data. The results demonstrated that many more proteins, higher sequence coverage, and even more microbial species and functional annotations could be identified using the Meta-database compared with those identified using the Public database. These findings indicated that the Meta-database was more specific as a protein sequence database. However, the follow-up in-depth metaproteomic analyses exhibited similar main results regardless of the database used. The microbial community composition at the genus level was similar between the two databases, especially the species annotations with high peptide-spectrum match and high abundance. The functional analyses in response to stress, such as the gene ontology enrichment of biological progress and molecular function and the key functional microorganisms, were also similar regardless of the database. Our analysis revealed that the Public database could also meet the demand to explore the functional responses of microbial proteins to some extent. This study provides valuable insights into the choice of protein sequence databases and their impacts on subsequent bioinformatic analysis in soil metaproteomic research and will facilitate the optimization of experimental design for different purposes.
基金The work is financially supported by the National Natural Science Foundation of China(Grant No.52070069)Fundamental Research Funds for the Central Universities(China)(No.B200202099)+3 种基金Jiangsu Province Natural Science Foundation(China)(BK20211207)Shanghai Post-doctoral Excellence Program(China)(No.2020419)China Postdoctoral Science Foundation(No.2021M692423)the State Key Laboratory of Pollution Control and Resource Reuse Foundation(China)(No.PCRRF20005).
文摘Surfactants were expected to exhibit positive effects on the waste activated sludge(WAS)disposal.However,the systematic comparison of different categories of surfactants on the WAS fermentation and the functional mechanisms,especially microbial metabolic traits,have not yet been precisely explored.This study revealed the positive effects of different surfactants on the volatile fatty acid(VFA)production,which followed the order of alkyl polysaccharides(APG)>sodium dodecylbenzene sulfonate(SDBS)>hexadecyl trimethyl ammonium bromide(HTAB).Mechanistic exploration found that the presence of different surfactants improved solubilization and hydrolysis steps,and then contributed to the subsequent acidification with different efficiencies.The functional microorganisms associated with VFA generation were enriched in surfactant-conditioned reactors.Metagenomic analysis further indicated that the key genes involved in the particular process of VFA generation were over-expressed.The simultaneous bioavailable substrate improvement,functional bacterial enrichment,and metabolic activity upregulation induced by different surfactants jointly contributed to VFA promotion during WAS fermentation.This study could provide a comprehensive realization of surfactants’impacts on theWAS fermentation process,and more importantly,it reminded the public to discern the distinct interplaying effects induced by different chemicals in regulating the WAS disposal and resource recovery.
