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Comparative Study of the Growth and Gonad Development of Clarias gariepinus (Burchell 1822) Fed Diets with Plant and Animal-based Ingredients in Concrete Tanks 被引量:1
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作者 Albert Philip Ekanem Sunday Urom Eteng +1 位作者 Francis Maduwuba Nwosu Victor Oscar Eyo 《Journal of Agricultural Science and Technology(A)》 2012年第10期1203-1210,共8页
A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus wi... A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus with an average weight of 205 ×5.09 g, were stocked in six concrete tanks (9 × 4 × 2 m3) containing 20 fish each. Experiment had 3 replicates and animals fed daily at 5% body weights. There was no significant difference (P 〈 0.05) in proximate compositions of the two experimental feeds and controls. Fecundity, gonad weight and gonasomatic index were higher in fish fed diet 13 than A and C. Similarly, growth indices were higher in fish fed diet B than in A and C. Histology of gonads showed a faster development of oocytes of eggs in fish fed animal-based ingredients than plant-based and combined plant and animal diets. Although there were slight differences in growth parameters and gonad development in favor of feed with animal-based ingredients, plant-based feed compared favorably in the growth and gonad development of C. gariepinus. Plant-based ingredients are recommended on the basis of affordability and availability as substitute for animal-based ingredients in C. gariepinus feed. 展开更多
关键词 Plant-based animal-based diets GROWTH gonad development Clarias gariepinus.
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Molecular Characterization, Expression Pattern and Transcriptional Regulation of Figla During Gonad Development in Japanese Founder (Paralichthys olivaceus)
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作者 QU Jiangbo LI Rui +5 位作者 LIU Yuxiang SUN Minmin YAN Weijie LIU Jinxiang WANG Xubo ZHANG Quanqi 《Journal of Ocean University of China》 SCIE CAS CSCD 2022年第4期1037-1050,共14页
The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in ... The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in teleosts remain unclear. Here,figla in P. olivaceus(Pofigla) was characterized with encoding a 202 amino acid protein that contains a conserved basic region and helix-loop-helix(HLH) domain. Amino acids alignment and synteny analysis revealed that Pofigla was conserved with the orthologous gene sequences in other vertebrates. The results of qRT-PCR showed Pofigla was maternally inherited during embryonic development. For tissue distribution, Pofigla showed a sexually dimorphic gene expression in the gonad of different genders, with a higher expression in ovary than in testis. In situ hybridization(ISH) results demonstrated Pofigla was specifically expressed in germ cells including oocytes, spermatogonia and spermatocytes. By screening and analyzing two proximal regions(-2966/-2126 and-772/-444) with high promoter activity, we found SOX5, LEF1, FOXP1 and GATA1 may play important roles in the transcriptional regulation of Pofigla. Furthermore, we observed the co-localization between Figla and LEF1 in HEK 293T cells. And the significant up-regulation effect of the canonical Wnt signaling pathway on the expression of Pofigla was found in cultured ovarian cells. This study provided the first evidence that figla not only has an important function in ovary development, but also plays some potential roles in testis development and/or male germ cell differentiation during early testis development in P. olivaceus. The results provide valuable reference in exploring the regulatory network of figla in teleost. 展开更多
关键词 figla transcriptional regulation Wnt signaling pathway gonad development Paralichthys olivaceus
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Gonad traits and age structure of the sea urchin Heliocidaris crassispina in central range:a comparison with rangeextenders
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作者 Wenping FENG Daichi NAKANO +3 位作者 Nobuyasu NAKABAYASHI Masakazu NAOKI Xiaofei TIAN Yukio AGATSUMA 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第5期1954-1964,共11页
The sea urchin Heliocidaris crassispina has northern expanded in geographic range to Toga Bay,Oga Peninsula in the Sea of Japan due to the global warming.To demonstrate the gonad traits and age structure of H.crassisp... The sea urchin Heliocidaris crassispina has northern expanded in geographic range to Toga Bay,Oga Peninsula in the Sea of Japan due to the global warming.To demonstrate the gonad traits and age structure of H.crassispina in the central range and compare the difference with those in northern extended range,we collected 100 H.crassispina individuals from a barren off Shitsumi,Fukui(central range),in August 2018.Their growth,gonad traits(size,development and color),and sex ratio were investigated and compared with those in Toga Bay,in August 2014(extended range).The successive 2012–2016 year classes of H.crassispina indicated successive juvenile recruitment in the central range,as found in the extended range.The individuals at three years old in central range were obviously larger than those in the extended range,showing large test diameter and body weight,possibly due to the higher temperature in central range than that in northern extended range.In addition,a balanced sex ratio in the central range was found,in contrast to the female-skewed sex ratio found in the extended range.Gonadal development was sex-and site-dependent.The testicular development in the central range was not only delayed in comparison to ovarian development,but also less delayed than those in the extended range,which possibly attribute to the higher water temperature in the central range.A significantly lower male gonad index and L*(lightness)value was recorded in central ranges compared to that in extended range,possibly due to the releasement of sperm of H.crassispina in central ranges. 展开更多
关键词 Heliocidaris crassispina age gonad development gonad color range extension
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Effects of spectral composition,photoperiod and light intensity on the gonadal development of Atlantic salmon Salmo salar in recirculating aquaculture systems(RAS) 被引量:4
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作者 仇登高 徐世宏 +5 位作者 宋昌斌 迟良 李贤 孙国祥 刘宝良 刘鹰 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第1期45-56,共12页
Artificial lighting regimes have been successfully used to inhibit sexual maturity of Atlantic salmon in confinement.However,when these operations are applied in commercial recirculating aquaculture systems(RAS) using... Artificial lighting regimes have been successfully used to inhibit sexual maturity of Atlantic salmon in confinement.However,when these operations are applied in commercial recirculating aquaculture systems(RAS) using standard lighting technology,sexual maturation is not suppressed.In this study,an L_9(3~3) orthogonal design was used to determine the effects of three factors(spectral composition,photoperiod,and light intensity) on the gonadal development of Atlantic salmon in RAS.We demonstrated that the photoperiod at the tested levels had a much greater effect on the gonadosomatic index and female Fulton condition factor than spectral composition and light intensity.The photoperiod had a significant effect on the secretion of sex steroids and melatonin(P<0.05),and a short photoperiod delayed sex steroid and melatonin level increases.The three test factors had no significant effects on the survival rate,specific growth rate,relative weight gain,and male Fulton condition factor(P>0.05).The optimum lighting levels in female and male Atlantic salmon were LD 8:16,455 nm(or 625 nm),8.60 W/m^2;and LD 8:16,8.60 W/m^2,455 nm respectively.These conditions not only delayed gonadal development,but also had no negative effects on Atlantic salmon growth in RAS.These results demonstrate that a combination of spectral composition,photoperiod and light intensity is effective at delaying the gonadal development of both male and female salmon in RAS. 展开更多
关键词 Salmo salar artificial light regimes gonadal development recirculating aquaculture systems (RAS)
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Annual change of the gonadal development of the amphioxus in Xiamen 被引量:2
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作者 Fang Yongqiang, Qi Xiang, Liang Ping and Hong GuiyingThird Institute of Oceanography, State Oceanic Administration, Xiamen, China 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1991年第3期477-479,共3页
The study was made by observing the annual change of the gonadal development of amphioxus in Xiamen cohtinuously for two years from November, 1986 to November, 1988. We determined the gonadal diameter, gonadosomatic i... The study was made by observing the annual change of the gonadal development of amphioxus in Xiamen cohtinuously for two years from November, 1986 to November, 1988. We determined the gonadal diameter, gonadosomatic index of each amphioxus, and observed the histological characteristic of the gonadal development of the female and male by cut section, which provided the basis for dividing the stages gonads and the determining level of the gonadal development of amphioxus in each month. 展开更多
关键词 Annual change of the gonadal development of the amphioxus in Xiamen
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Morphological and histological changes in the brains of turbot(Scophthalmus maximus)with gonadal development
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作者 Chunyan Zhao Liang Chi +5 位作者 Yongshuang Xiao Bing Li Yunliang Lu Yanting Cui Wenqi Wang Jun Li 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2022年第12期115-122,共8页
The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and... The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes. 展开更多
关键词 TURBOT BRAIN PITUITARY gonadal development morphology HISTOLOGY
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Hypothalamic-Pituitary-Gonadal(HPG)Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder(Paralichthys olivaceus)
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作者 NI Feifei YU Haiyang +7 位作者 QU Jiangbo MENG Lihui LIU Xiumei YAN Weijie CHANG Jing ZHANG Quanqi WANG Xubo YU Haiyang 《Journal of Ocean University of China》 SCIE CAS CSCD 2020年第6期1378-1388,共11页
The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline maintenance.In this study,we identified and char... The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline maintenance.In this study,we identified and characterized the Paralichthys olivaceus piwil2 gene,a constituent factor of the piRNA pathways involved in the biogenesis of reproductive development.The biological analysis indicated that piwil2,which contains PAZ and PIWI domains,was highly conserved between teleosts and tetrapods.The piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern,with a higher expression level in testis.In situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the testes.Gene piwil2 showed a maternally inherited expression pattern during embryonic development,and was highly expressed during the early embryonic development.Different luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of piwil2.The piwil2 core promoter region was located at−360 bp to−60 bp.Furthermore,some representative sex hormones,including human chorionic gonadotropin,17α-methyltestosterone,and estradiol-17βhad distinct regulatory effects on piwil2.In a summery,these results indicate that piwil2,regulated by sex hormones and transcriptional elements,has vital functions in the reproductive cycle and gonadal development. 展开更多
关键词 piwil2 gametogenesis and gonadal development HPG axis transcriptional regulatory elements Paralichthys olivaceus
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克氏原螯虾PcCRCN-L基因的克隆鉴定及在低氧胁迫下的表达响应
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作者 韩一鸣 鲁苏皖 +5 位作者 许志强 徐宇 林海 潘建林 杨家新 李旭光 《渔业科学进展》 CSCD 北大核心 2024年第1期128-137,共10页
虾青蛋白(Crustacyanin,CRCN)在甲壳动物脂类代谢及低氧胁迫应激调控等方面具有重要的功能。为获取虾青蛋白在克氏原螯虾(Procambarus clarkii)性腺发育和低氧胁迫应答中的作用,本研究在克氏原螯虾肝胰腺组织中鉴定出1个类虾青蛋白PcCRC... 虾青蛋白(Crustacyanin,CRCN)在甲壳动物脂类代谢及低氧胁迫应激调控等方面具有重要的功能。为获取虾青蛋白在克氏原螯虾(Procambarus clarkii)性腺发育和低氧胁迫应答中的作用,本研究在克氏原螯虾肝胰腺组织中鉴定出1个类虾青蛋白PcCRCN-L基因的c DNA序列,分析了PcCRCN-L基因的结构特征和进化模式,研究了PcCRCN-L基因在不同组织与性腺不同发育时期的表达特征,探讨了Pc CRCN-L在低氧–复氧胁迫下的表达响应模式。结果显示,PcCRCN-L基因c DNA序列长2700 bp,其开放阅读框(ORF)长度为1587 bp,编码528个氨基酸残基;DNA序列长6130 bp,位于克氏原螯虾基因组的第12号染色体,包含5个外显子和4个内含子,内含子/外显子剪接方式符合GT-AG规则。Pc CRCN-L具有1个完整的lipocalin结构域,包含典型的序列保守区Ⅰ(SCR1)序列G-X-W、保守区Ⅱ(SCR2)序列T-D-Y和保守区Ⅲ(SCR3)序列精氨酸R。多序列比对与系统进化分析结果显示,PcCRCN-L独立于传统虾青蛋白A和C亚族,单独聚为一支。Pc CRCN-L在克氏原螯虾多个组织中均有表达,在肝胰腺中表达量最高;在性腺不同发育时期,肝胰腺以及卵巢组织中的Pc CRCN-L基因表达量显著降低;低氧胁迫下,肝胰腺组织中Pc CRCN-L表达量显著降低,复氧后显著升高。研究结果表明,PcCRCN-L与克氏原螯虾性腺发育密切相关,并参与了克氏原螯虾的低氧–复氧胁迫应激调控。 展开更多
关键词 克氏原螯虾 类虾青蛋白 组织表达 性腺不同发育时期 低氧胁迫
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Advances in Growth and Development in Crustaceans Induced by Eyestalk Ablation
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作者 Yan SHUI Yonghong SHI +1 位作者 Zenghong XU Xin ZHOU 《Agricultural Biotechnology》 CAS 2014年第4期28-30,共3页
Eyestalk ablation has been widely used in artificial breeding of crustaceans due to its significant promotion effect on gonadal development, which plays a certain role by directly affecting neuroendocrine function of ... Eyestalk ablation has been widely used in artificial breeding of crustaceans due to its significant promotion effect on gonadal development, which plays a certain role by directly affecting neuroendocrine function of X-organ sinus gland (XO-SG) complex in eyestalks. As an important endocrine organ, XO-SG complex of the eyestalk has become a hot issue in crustacean endocrinology. This paper introduced the biological structure and physiological functions of XO-SG complex and summarized the molecular mechanism of eyestalk neuroendocrine, which provided theoretical references for further revealing the regulatory mechanism of eyestalk neuroendocrine system. 展开更多
关键词 Eyestalk- ablation XO-SG gonadal development CHH
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黄海北部虾夷扇贝性腺发育及繁殖规律研究 被引量:7
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作者 李文姬 滕炜鸣 +5 位作者 王笑月 徐高蓉 李华琳 鲁学辉 刘项峰 于佐安 《水产科学》 CAS 北大核心 2012年第12期703-707,共5页
通过性腺指数周年监测及性腺的组织切片观察,对黄海北部虾夷扇贝性腺发育及繁殖规律进行了研究。试验结果表明,虾夷扇贝性腺发育可划分为5期,即增殖期(Ⅰ期)、生长期(Ⅱ期)、成熟期(Ⅲ期)、排放期(Ⅳ)和休止期(Ⅴ期)。性腺周年变化规律... 通过性腺指数周年监测及性腺的组织切片观察,对黄海北部虾夷扇贝性腺发育及繁殖规律进行了研究。试验结果表明,虾夷扇贝性腺发育可划分为5期,即增殖期(Ⅰ期)、生长期(Ⅱ期)、成熟期(Ⅲ期)、排放期(Ⅳ)和休止期(Ⅴ期)。性腺周年变化规律是,11—12月为增殖期,1—2月为生长期,3月为成熟期、4—5月为排放期,6—10月为休止期。水温对性腺发育和产卵期的影响是,水温低性腺指数峰值高,产卵期相对滞后;水温高性腺指数峰值低,产卵期相对提早。 展开更多
关键词 虾夷扇贝 性腺发育 性腺分期 繁殖规律
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马粪海胆卵巢发育周年变化研究 被引量:3
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作者 曾晓起 袁修宝 张鹏 《海洋科学》 CAS CSCD 北大核心 2005年第12期38-42,共5页
利用光镜和电镜观察研究了马粪海胆(Hemicentrotus pulcherrimus)卵巢组织的周年变化,同时逐月测定了海胆的生殖腺指数.在此基础上将生殖腺的发育周期划分为五期:恢复期、生长期、成熟前期、成熟期、排放后期.实验结果表明,青岛近岸马... 利用光镜和电镜观察研究了马粪海胆(Hemicentrotus pulcherrimus)卵巢组织的周年变化,同时逐月测定了海胆的生殖腺指数.在此基础上将生殖腺的发育周期划分为五期:恢复期、生长期、成熟前期、成熟期、排放后期.实验结果表明,青岛近岸马粪海胆的产卵期为4~6月. 展开更多
关键词 马粪海胆(Hemicentrotus pulcherrimus) 卵巢 生殖腺指数 发育周期
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Interspecific Hybridization Between Crassostrea angulata and C. ariakensis 被引量:2
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作者 YAO Tuo ZHANG Yuehuan +4 位作者 YAN Xiwu WANG Zhaoping LI Dongchun SU Jiaqi YU Ruihai 《Journal of Ocean University of China》 SCIE CAS 2015年第4期710-716,共7页
lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish sp... lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish species in China. We conducted 2 × 2 reciprocal crosses to determine whether these two species can cross-fertilize and their hybrids can hatch, survive and perform hetero- sis. Fertilization was found symmetrical without delay. The rate of fertilization success of C. angulata ♀× C. ariakensis ♂ was lower than that of C. ariakensis ♀ x C. angulata ♂, and the success rate of both hybridizations was lower than that of two intraspecific crosses each. During the planktonic period, survival rate of the progeny was lower in the hybrid crosses than in the intraspecific crosses. On day 360, mean shell height of the progeny of C. angulata ♀ x C. angulata ♂ was highest, which was followed by that of C. angulata ♀ × C. ariakensis ♂, C. ariakensis ♀x C. ariakensis ♂ and C. ariakensis ♀× C. angulata ♂ in a descending order. Mor- phology of adults produced by the hybrid crosses was similar to that of C. angulata. Both hybrids underwent normal gonad devel- opment and produced mature gametes in the mating season. This study provided new insights into the quantitative traits in interspeci- fic crosses of Crassostrea species, thus being of guidance value for selective breeding of oyster. 展开更多
关键词 Crassostrea angulata Crassostrea ariakensis interspecific hybridization gonad development HETEROSIS
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Effect of temperature and photoperiod on maturation of Cephalothrix hongkongiensis(Nemertea: Palaeonemertea)
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作者 李应东 宋肖跃 +2 位作者 张珊珊 朱林 孙世春 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第4期764-772,共9页
The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few sp... The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4~C and 6~C showed no growth or gonadal development, and all died within 109 days. At 8~C and 12~C, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28~C showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature (16℃), photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis. 展开更多
关键词 TEMPERATURE PHOTOPERIOD gonad development REPRODUCTION Cephalothrix hongkongiensis NEMERTEA
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Study on Early Propagation Technology of Yellow Catfish (Pelteobagrus fulvidraco)
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作者 Heng ZHANG Guoliang RUAN +2 位作者 Kun GUO Wenhao FAN Weiyou ZHENG 《Agricultural Biotechnology》 CAS 2020年第1期63-66,69,共5页
[Objectives] This study was conducted to study early reproduction techniques of yellow catfish(Pelteobagrus fulvidraco) in Jianghan Plain. [Methods] Parents of P. fulvidraco were cultured by the methods of artificial ... [Objectives] This study was conducted to study early reproduction techniques of yellow catfish(Pelteobagrus fulvidraco) in Jianghan Plain. [Methods] Parents of P. fulvidraco were cultured by the methods of artificial temperature control and nutrition enhancement. [Results] After culture, the body weight, gonad weight, maturity coefficient and egg diameter of P. fulvidraco increased gradually. Up to April 30, the gonad weight of the female parents of P. fulvidraco cultured in greenhouses was significantly higher than that of the parents under natural conditions(P<0.05), 54% higher than that of the parents naturally cultured at the same period;the maturity coefficient of the parents cultured under strengthened conditions was also significantly higher than that of the parents under natural conditions at the same period(P<0.05);and the ovaries of the strengthened parents were orange yellow and full of eggs, and the number of eggs per fish was relatively high. In early May, the parents cultured under strengthened conditions were induced to spawn artificially. The average induced spawning rate and fertilization rate reached 86.7% and 71.6%, respectively, reaching the respective normal level. The gonad maturity of P. fulvidraco cultured under strengthened conditions was 15 d earlier than that under natural conditions, resulting in mass production of early-propagated larvae of P. fulvidraco. [Conclusions] In Jianghan plain area, artificial propagation can be achieved in early May by means of artificial temperature control and strengthened culture, which is 20 d ahead of natural conditions. 展开更多
关键词 Pelteobagrus fulvidraco Early propagation gonad development
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牙鲆Lhx1基因的克隆和表达分析 被引量:1
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作者 翁申达 尤锋 +2 位作者 范兆飞 文爱韵 王丽娟 《海洋科学》 CAS CSCD 北大核心 2013年第11期1-8,共8页
Lhx1基因对于哺乳动物卵巢缪勒氏管的形成具有重要作用,但鱼类中的报道仅限于其在体轴和肾脏形成中的作用,尚未见到其与性别及性腺发育相关的报道。本研究克隆获得了牙鲆(Paralichthys olivaceus)Lhx1a和Lhx1b开放阅读框(ORF)序列,长度... Lhx1基因对于哺乳动物卵巢缪勒氏管的形成具有重要作用,但鱼类中的报道仅限于其在体轴和肾脏形成中的作用,尚未见到其与性别及性腺发育相关的报道。本研究克隆获得了牙鲆(Paralichthys olivaceus)Lhx1a和Lhx1b开放阅读框(ORF)序列,长度分别为1224 bp和1206 bp(GenBank注册号为:JX999940、JX999941),同源序列比较显示牙鲆Lhx1a和Lhx1b基因均具有两个LIM及一个HOX结构域,符合Lhx1进化保守性。这两个基因在牙鲆雌、雄成鱼各组织RT-PCR差异表达谱不尽相同,Lhx1a在精巢中弱表达,卵巢中不表达,在其他组织中雌、雄表达谱差异不大,都只在脑和眼组织有弱表达;而Lhx1b的表达在性腺中相对较高,且卵巢的表达量高于精巢。进一步检测牙鲆Lhx1a和Lhx1b在雌、雄性腺发育各期的表达谱,发现这两个基因表达集中于Ⅲ、Ⅳ期性腺,Lhx1a在精巢中弱表达,Lhx1b卵巢中的表达明显高于精巢,而在Ⅰ、Ⅱ、Ⅱ、Ⅴ期性腺几乎均不表达。由此推断牙鲆Lhx1a和Lhx1b均为性别相关基因,但在两性性腺发育中的作用可能各有不同。 展开更多
关键词 Lhx1基因 牙鲆(Paralichthys olivaceus) RT-PCR 组织 性腺发育期
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Impacts of different feeding modes on the gonadal development, total edible yield, and nutritional composition of male Chinese mitten crab (Eriocheir sinensis) 被引量:8
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作者 Jie Pan Renfu Wu +3 位作者 Xugan Wu Xiaowen Long Xichang Wang Yongxu Cheng 《Aquaculture and Fisheries》 2020年第6期300-307,共8页
This study analyzed the tissue indices,proximate composition,fatty acid and amino acid composition of edible tissues of male E.sinensis under three feeding modes(formulated diets(FD),natural diets(ND),and traditional ... This study analyzed the tissue indices,proximate composition,fatty acid and amino acid composition of edible tissues of male E.sinensis under three feeding modes(formulated diets(FD),natural diets(ND),and traditional diets(TD)).The results showed that different feeding modes had no impacts on the overall gonadal development,as no significant difference was found in gonadsomatic index among three treatments.For edible yield,three modes all had similar levels of the meat yield,hepatosomatic index,and total edible yield.Although different feeding modes did not affect the individual edible yield and gonadal development,significant differences were detected in the nutritional composition of males from three modes.For proximate composition,significant differences were found in the total lipids of gonads and the carbohydrate of hepatopancreas and meat.For fatty acids,crabs from ND had a higher proportion of monounsaturated fatty acids.In gonads and hepatopancreas,FD had a higher proportion of long-chain polyunsaturated fatty acids.For amino acids,FD had higher amino acid contents,while ND had the lowest.Except tryptophan,the essential amino acids scores of the meat were all greater than 100.As there was no significant difference on meat quality and meat yields between three feeding modes,this study suggests that the formulated diet could replace the natural diet and traditional diet towards sustainable crab farming in future. 展开更多
关键词 Eriocheir sinensis Feeding modes Edible yield gonadal development
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Pod1 is involved in the sexual differentiation and gonadal development of the Nile tilapia 被引量:2
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作者 LI JianZhong LIU ShaoJun NAGAHAMA Yoshitaka 《Science China(Life Sciences)》 SCIE CAS 2011年第11期1005-1010,共6页
Pod1 is a member of the basic helix-loop-helix(bHLH) family of transcription factors that have been implicated in the regulation of sexual differentiation and gonadal development in mammals.However,to date,little is k... Pod1 is a member of the basic helix-loop-helix(bHLH) family of transcription factors that have been implicated in the regulation of sexual differentiation and gonadal development in mammals.However,to date,little is known about the role of Pod1 in nonmammalian vertebrate gonadogenesis.We cloned and characterized the Pod1 gene from tilapia.The tilapia Pod1 gene contains an open reading frame(ORF) of 525 nucleotides which potentially codes for a protein with 174 amino acids.Sequence alignment revealed that the deduced tilapia protein sequence shared high homology(79.5% to 90.5%) with the Pod1 sequences of other vertebrates.The tissue distribution of Pod1 revealed by RT-PCR showed that it had varied expression patterns in adult tilapia.In situ hybridization was performed to examine the temporal and spatial expression patterns of Pod1 during tilapia sexual differentiation and gonadal development.In the undifferentiated gonad,Pod1 was expressed in the somatic cells of both sexes.Subsequently,Pod1 expression in tilapia persisted in differentiated juvenile and adult ovary and testis.Our data indicate for the first time that Pod1 is not only necessary for the onset of sexual differentiation,but also plays an important role in gonadal development in the teleost. 展开更多
关键词 Pod1 somatic cells sexual differentiation gonadal development TILAPIA
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Environmental hypoxia:A threat to the gonadal development and reproduction in bony fishes 被引量:3
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作者 Nirmalendu Saha Debaprasad Koner Ritu Sharma 《Aquaculture and Fisheries》 2022年第5期572-582,共11页
Vast stretches of open water bodies are gradually becoming hypoxic as a result of depletion of oxygen levels mainly due to various human anthropogenic activities.This problem of hypoxic stress on the fish population i... Vast stretches of open water bodies are gradually becoming hypoxic as a result of depletion of oxygen levels mainly due to various human anthropogenic activities.This problem of hypoxic stress on the fish population is likely to be exacerbated soon since the aquatic hypoxic environment is continuously spreading over vast areas worldwide.In recent years,various harmful effects of hypoxia to bony fishes have been reported,such as the restriction of energy-consuming metabolic processes,arrest of the growth of ovary and testes that are associated with endocrine disruption,loss of sperm and egg quality,inhibition of fertilization,hatching success,and also the reduction of larval survivability,thereby impairment of overall reproductive and developmental processes in fish.Disruption of the brain-pituitary-gonad axis,and certain enzymes related to steroidogenesis and vitellogenesis in fish have also been reported as the primary targets for an endocrine malfunction during hypoxia.Hypoxia-sensitive downregulation of key genes responsible for controlling sex hormones’synthesis has been documented in certain bony fishes.Further,continuous exposure to hypoxia was reported to induce early expression of pro-apoptotic/tumor suppressor p53 genes,thereby causing immense cell death in hypoxic embryos.However,the cellular responses to long-term hypoxia exposure and the degree of reproductive impairments in bony fishes are still not adequate to figure out the actual underlying mechanisms.The present review intends to highlight the current knowledge about the detrimental impact of chronic/acute hypoxia at different stages of fish reproduction and the associated underlying molecular mechanisms. 展开更多
关键词 Sex hormones Endocrine disruption GAMETOGENESIS gonadal development Fish reproduction HPG-Axis
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暗纹东方鲀芳香化酶基因的结构及表达分析 被引量:3
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作者 高莹莹 刘新富 +2 位作者 胡鹏 黄滨 刘滨 《水产科学》 CAS CSCD 北大核心 2020年第1期30-39,共10页
性腺型芳香化酶基因是一种调节雌雄激素平衡的基因。为初步探究暗纹东方鲀性腺型芳香化酶基因在性腺发育和性别分化过程中的作用,通过RACE及荧光定量PCR技术,成功克隆出暗纹东方鲀性腺型芳香化酶基因的cDNA全长序列,共1786 bp,编码514... 性腺型芳香化酶基因是一种调节雌雄激素平衡的基因。为初步探究暗纹东方鲀性腺型芳香化酶基因在性腺发育和性别分化过程中的作用,通过RACE及荧光定量PCR技术,成功克隆出暗纹东方鲀性腺型芳香化酶基因的cDNA全长序列,共1786 bp,编码514个氨基酸,与红鳍东方鲀及星点东方鲀同源性最高。氨基酸序列分析显示,该基因编码的蛋白是一种稳定的亲水性蛋白,但不存在信号肽序列;性腺型芳香化酶含有38个磷酸化位点、4个N-糖基化位点、2个跨膜保守结构域;其氨基酸序列在哺乳动物和鱼类中均十分保守,且含有跨膜区、Ⅰ-螺旋区、Ozol′s肽区、芳香化酶特异性保守区和亚铁血红素结合区等功能保守区。表达分析显示,暗纹东方鲀性腺型芳香化酶基因主要在肌肉和卵巢中表达,其次在其他组织中也有少量表达,而且在幼鱼不同发育期卵巢中的表达量呈现出逐渐升高再降低的表达趋势,在精巢中则基本不表达。研究结果表明,性腺型芳香化酶基因很可能参与了暗纹东方鲀雌鱼性腺分化后卵巢的发育、雌性特征的维持和其他组织的发育等过程。 展开更多
关键词 暗纹东方鲀 性腺型芳香化酶基因 CDNA克隆 生物信息学 组织表达 发育期
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Gonadal development and silvering of the Japanese Eel(Anguilla japonica)in the Yangtze River during downstream migration 被引量:1
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作者 Xiaoling Gong Dehai Wang +3 位作者 Baolong Bao Qian Zhang Xinglong Jiang Liping Liu 《Aquaculture and Fisheries》 2017年第4期173-178,共6页
The Yangtze River and its branches are important habitats for the Japanese Eel(Anguilla japonica),however,there are few data on gonadal development and silvering indices of eels during the downstream spawning migratio... The Yangtze River and its branches are important habitats for the Japanese Eel(Anguilla japonica),however,there are few data on gonadal development and silvering indices of eels during the downstream spawning migration.This study investigates gonadal development in 121 Japanese eels caught downstream from the Jingjiang Reach of the Yangtze River in October 2012.We found that in females the ovaries of most of the out-migrating population developed earlier than the testes in males,that is,the ovaries reached stage 3(F3)or early stage 4(eF4),while the testes were at stage 2(M2)or stage 3(M3).Gonadal development in eels collected in 2012 was earlier than that of samples caught from the same region in 2007e2008 and from the Pearl River Estuary in 1972e1973.The average age,body size and gonadal-somatic index of females were significantly greater than those in males.For both females and males,there were no significant differences between the two stages of gonadal development in any of the morphological indices,which indicates that gonadal development of the Japanese Eel is independent of body size.These results help us to understand the spawning population of Japanese Eels in the Yangtze River. 展开更多
关键词 Japanese eel Anguilla japonica Spawning migration gonadal development Morphological indices
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