目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cel...目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cell tumor of bone,NGCTB)(包含14例动脉瘤样骨囊肿、16例软骨母细胞瘤和53例非骨化性纤维瘤)患者的样本和病历资料,采用免疫组织化学EliVision法检测H3.3G34W、p63及SATB2的表达情况。通过χ^(2)检验判断H3.3G34W、p63及SATB2的阳性率在各组间是否存在统计学差异;通过Logistic回归分析建立包括H3.3G34W、p63及SATB2的联合诊断模型,通过受试者工作特征(ROC)曲线分析评价模型的诊断价值。结果H3.3G34W、p63及SATB2在GCTB组中阳性率分别为81.5%、90.7%、92.6%;在NGCTB组中阳性率分别为2.4%、28.9%、62.7%。与NGCTB组相比,GCTB组患者年龄显著较大[(41.222±14.849)vs.(16.566±9.439);P<0.001],女性比男性患病率更高(51.9%vs.48.1%,P<0.001)。与NGCTB组相比,GCTB组中H3.3G34W(81.5%vs.2.4%,P<0.001);p63(90.7%vs.28.9%,P<0.001)和SATB2(92.6%vs.62.7%,P<0.001)的阳性率更高。单因素Logistic回归分析构建单因素预测模型,同时行ROC曲线分析,表明年龄(AUC=92.9%,P<0.001)、性别(AUC=64.5%,P=0.004)、H3.3G34W阳性率(AUC=89.5%,P<0.001)、p63阳性率(AUC=80.9%,P<0.001)、SATB2阳性率(AUC=65.0%,P=0.003)是GCTB诊断的独立预测因素。进一步的多因素Logistic回归分析构建混合预测模型,并行ROC曲线分析,发现混合模型展现出比单因素模型更好的预测价值(AUC=98.4%,P<0.001)。结论H3.3G34W、p63及SATB2是有效诊断GCTB的分子标记物,且三者联合应用更能提高GCTB的诊断预测效能。展开更多
The molecular recyclability of poly (ethylene terephthalate) (PET) and three semi-aromatic polyesters poly (phloretic acid) (poly-H), poly (dihydroferulic acid) (poly-G), and poly (dihydrosinapinic acid) (poly-S) is e...The molecular recyclability of poly (ethylene terephthalate) (PET) and three semi-aromatic polyesters poly (phloretic acid) (poly-H), poly (dihydroferulic acid) (poly-G), and poly (dihydrosinapinic acid) (poly-S) is evaluated in this study. PET is an extensively used aromatic polyester, and poly-H, poly-G, and poly-S can be considered semi-aromatic poly (lactic acid) modifications. All these polyesters have been depolymerized at neutral pH and by acid- and base-catalyzed hydrolysis at two temperatures, i.e., 50˚C and 80˚C. Base-catalyzed depolymerization of virgin PET leads to an isolated yield of 38% after 48 hours of reaction at 80˚C. Contrary to these results for PET, almost all the monomers of the semi-aromatic polyesters poly-H, poly-G, and poly-S are recovered with isolated yields larger than 90% at the same temperature after 15 minutes in a facile manner. A shrinking particle model used to determine the global kinetics of the base-catalyzed depolymerization showed that the rate rises with increasing temperature. Using the shrinking particle model, the intrinsic reaction rate constants were determined. It has been demonstrated that the rate coefficients of the depolymerization of the semi-aromatic polyesters poly-H, poly-G, and poly-S are between 2 and 3 orders of magnitude higher than those for PET.展开更多
目的:探讨结直肠癌(colorectal cancer,CRC)中非染色体结构维持蛋白凝缩蛋白复合体I亚单位H(non-SMC condensin I complex subunit H,NCAPH)、G补缀FHA域血管新生因子1(angiogenic factor with G and FHA domains 1,AGGF1)及跨膜4L六家...目的:探讨结直肠癌(colorectal cancer,CRC)中非染色体结构维持蛋白凝缩蛋白复合体I亚单位H(non-SMC condensin I complex subunit H,NCAPH)、G补缀FHA域血管新生因子1(angiogenic factor with G and FHA domains 1,AGGF1)及跨膜4L六家族成员1(transmembrane-4-L-six-family-1,TM4SF1)蛋白质表达之间的关系及临床意义。方法:收集145例CRC术后标本和30例癌旁正常黏膜组织标本,采用免疫组织化学法检测CRC和癌旁正常黏膜组织中NCAPH、AGGF1及TM4SF1蛋白质的表达情况,分析其表达与各种临床病理因素的关系以及三者之间的相关性。结果:在CRC和癌旁组织中,NCAPH、AGGF1及TM4SF1的阳性表达率分别为55.2%、53.1%、60.7%和3.3%、6.6%、0,差异均有统计学意义(均P<0.001)。3种蛋白质的表达均与CRC的组织学分化和TNM分期有关(均P<0.001);NCAPH和TM4SF1的表达均与CRC的淋巴结转移有关(均P<0.05);NCAPH和AGGF1的表达均与CRC组织脉管侵犯有关(均P<0.05);AGGF1和TM4SF1的表达均与CRC的肿瘤浸润深度有关(均P<0.01)。TM4SF1的表达分别与NCAPH和AGGF1的表达呈正相关(r值分别为0.311和0.517,均P<0.001);同时,AGGF1与NCAPH的表达亦呈正相关(r=0.291,P=0.001)。Kaplan-Meier生存分析表明:NCAPH、AGGF1及TM4SF1的表达上调均与患者的生存率有关,NCAPH、AGGF1及TM4SF1阳性的患者生存率明显低于三者阴性患者(均P<0.05)。多因素分析表明:TNM分期、NCAPH、AGGF1及TM4SF1的表达和肿瘤脉管侵犯均是影响CRC根治术后患者预后的独立因素(均P<0.05)。结论:CRC组织中NCAPH、AGGF1及TM4SF1的表达上调与CRC的分化程度、转移和预后等因素相关,这些指标的联合检测可能作为判断CRC进展及患者预后的重要指标。展开更多
文摘目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cell tumor of bone,NGCTB)(包含14例动脉瘤样骨囊肿、16例软骨母细胞瘤和53例非骨化性纤维瘤)患者的样本和病历资料,采用免疫组织化学EliVision法检测H3.3G34W、p63及SATB2的表达情况。通过χ^(2)检验判断H3.3G34W、p63及SATB2的阳性率在各组间是否存在统计学差异;通过Logistic回归分析建立包括H3.3G34W、p63及SATB2的联合诊断模型,通过受试者工作特征(ROC)曲线分析评价模型的诊断价值。结果H3.3G34W、p63及SATB2在GCTB组中阳性率分别为81.5%、90.7%、92.6%;在NGCTB组中阳性率分别为2.4%、28.9%、62.7%。与NGCTB组相比,GCTB组患者年龄显著较大[(41.222±14.849)vs.(16.566±9.439);P<0.001],女性比男性患病率更高(51.9%vs.48.1%,P<0.001)。与NGCTB组相比,GCTB组中H3.3G34W(81.5%vs.2.4%,P<0.001);p63(90.7%vs.28.9%,P<0.001)和SATB2(92.6%vs.62.7%,P<0.001)的阳性率更高。单因素Logistic回归分析构建单因素预测模型,同时行ROC曲线分析,表明年龄(AUC=92.9%,P<0.001)、性别(AUC=64.5%,P=0.004)、H3.3G34W阳性率(AUC=89.5%,P<0.001)、p63阳性率(AUC=80.9%,P<0.001)、SATB2阳性率(AUC=65.0%,P=0.003)是GCTB诊断的独立预测因素。进一步的多因素Logistic回归分析构建混合预测模型,并行ROC曲线分析,发现混合模型展现出比单因素模型更好的预测价值(AUC=98.4%,P<0.001)。结论H3.3G34W、p63及SATB2是有效诊断GCTB的分子标记物,且三者联合应用更能提高GCTB的诊断预测效能。
文摘The molecular recyclability of poly (ethylene terephthalate) (PET) and three semi-aromatic polyesters poly (phloretic acid) (poly-H), poly (dihydroferulic acid) (poly-G), and poly (dihydrosinapinic acid) (poly-S) is evaluated in this study. PET is an extensively used aromatic polyester, and poly-H, poly-G, and poly-S can be considered semi-aromatic poly (lactic acid) modifications. All these polyesters have been depolymerized at neutral pH and by acid- and base-catalyzed hydrolysis at two temperatures, i.e., 50˚C and 80˚C. Base-catalyzed depolymerization of virgin PET leads to an isolated yield of 38% after 48 hours of reaction at 80˚C. Contrary to these results for PET, almost all the monomers of the semi-aromatic polyesters poly-H, poly-G, and poly-S are recovered with isolated yields larger than 90% at the same temperature after 15 minutes in a facile manner. A shrinking particle model used to determine the global kinetics of the base-catalyzed depolymerization showed that the rate rises with increasing temperature. Using the shrinking particle model, the intrinsic reaction rate constants were determined. It has been demonstrated that the rate coefficients of the depolymerization of the semi-aromatic polyesters poly-H, poly-G, and poly-S are between 2 and 3 orders of magnitude higher than those for PET.
文摘目的:探讨结直肠癌(colorectal cancer,CRC)中非染色体结构维持蛋白凝缩蛋白复合体I亚单位H(non-SMC condensin I complex subunit H,NCAPH)、G补缀FHA域血管新生因子1(angiogenic factor with G and FHA domains 1,AGGF1)及跨膜4L六家族成员1(transmembrane-4-L-six-family-1,TM4SF1)蛋白质表达之间的关系及临床意义。方法:收集145例CRC术后标本和30例癌旁正常黏膜组织标本,采用免疫组织化学法检测CRC和癌旁正常黏膜组织中NCAPH、AGGF1及TM4SF1蛋白质的表达情况,分析其表达与各种临床病理因素的关系以及三者之间的相关性。结果:在CRC和癌旁组织中,NCAPH、AGGF1及TM4SF1的阳性表达率分别为55.2%、53.1%、60.7%和3.3%、6.6%、0,差异均有统计学意义(均P<0.001)。3种蛋白质的表达均与CRC的组织学分化和TNM分期有关(均P<0.001);NCAPH和TM4SF1的表达均与CRC的淋巴结转移有关(均P<0.05);NCAPH和AGGF1的表达均与CRC组织脉管侵犯有关(均P<0.05);AGGF1和TM4SF1的表达均与CRC的肿瘤浸润深度有关(均P<0.01)。TM4SF1的表达分别与NCAPH和AGGF1的表达呈正相关(r值分别为0.311和0.517,均P<0.001);同时,AGGF1与NCAPH的表达亦呈正相关(r=0.291,P=0.001)。Kaplan-Meier生存分析表明:NCAPH、AGGF1及TM4SF1的表达上调均与患者的生存率有关,NCAPH、AGGF1及TM4SF1阳性的患者生存率明显低于三者阴性患者(均P<0.05)。多因素分析表明:TNM分期、NCAPH、AGGF1及TM4SF1的表达和肿瘤脉管侵犯均是影响CRC根治术后患者预后的独立因素(均P<0.05)。结论:CRC组织中NCAPH、AGGF1及TM4SF1的表达上调与CRC的分化程度、转移和预后等因素相关,这些指标的联合检测可能作为判断CRC进展及患者预后的重要指标。
