Wound management is an important issue that places enormous pressure on the physical and mental health of patients,especially in cases of infection,where the increased inflammatory response could lead to severe hypert...Wound management is an important issue that places enormous pressure on the physical and mental health of patients,especially in cases of infection,where the increased inflammatory response could lead to severe hypertrophic scars(HSs).In this study,a hydrogel dressing was developed by combining the high strength and toughness,swelling resistance,antibacterial and antioxidant capabilities.The hydrogel matrix was composed of a double network of polyvinyl alcohol(PVA)and agarose with excellent mechanical properties.Hyperbranched polylysine(HBPL),a highly effective antibacterial cationic polymer,and tannic acid(TA),a strong antioxidant molecule,were added to the hydrogel as functional components.Examination of antibacterial and antioxidant properties of the hydrogel confirmed the full play of the efficacy of HBPL and TA.In the in vivo studies of methicillin-resistant Staphylococcus aureus(MRSA)infection,the hydrogel had shown obvious promotion of wound healing,and more profoundly,significant suppression of scar formation.Due to the common raw materials and simple preparation methods,this hydrogel can be mass produced and used for accelerating wound healing while preventing HSs in infected wounds.展开更多
Rapid post-wound closure is necessary to avoid wound infection and promote scar-free healing when skin trauma occurs.In this study,new types of hydrogel dressings with adjustable contractility were fabricated based on...Rapid post-wound closure is necessary to avoid wound infection and promote scar-free healing when skin trauma occurs.In this study,new types of hydrogel dressings with adjustable contractility were fabricated based on N-isopropyl acrylamide/sodium alginate/graphene oxide(P/SA/GO).Then,the chitosan(CS)solution was used as a bridging polymer to achieve tissue adhesion to the hydrogel.The results show that the hydrogel based on poly(N-isopropyl acrylamide)(PNIPAM)not only has the ability to self-shrink but also can adjust the rate of shrinkage through near-infrared thermal stimulation.At the same time,high adhesion strength(7.86±1.22 kPa)between the tissue and the dressing is achieved through the introduction of bridging polymers(CS),and the coating area of the bridging polymer can be adjusted to achieve regional adhesion.The mouse total skin defects experiments have shown that sutures-free wound closure in the early stages of wound healing could be obtained by adjusting the material temperature.Besides,the dressings can promote scar-free wound healing by reducing inflammatory cell infiltration and collagen deposition.These results indicate that double-crosslinked PNIPAM-based hydrogel dressings with adjustable adhesion and contractility proposed in this study provide a candidate material for achieving trackless wound healing.展开更多
Burn wounds are destructive skin traumas typically of irregular shape and large area. Prone to infection, they require frequent dressing replacement, and painless removal of dressings from burn wounds remains a major ...Burn wounds are destructive skin traumas typically of irregular shape and large area. Prone to infection, they require frequent dressing replacement, and painless removal of dressings from burn wounds remains a major challenge. This study focuses on the dynamic characteristics and treatment difficulty of burn wounds. Hydrogel dressings based on glycol chitosan and propionaldehyde-or benzaldehyde-terminated 4-arm poly(ethylene glycol) were designed on the basis of Schiff base cross-linking networks. The hydrogels exhibited shape-adaptability, self-healing and fast-degradation properties, which makes these hydrogels suitable for burn wounds. Salvianolic acid B(SaB)-loaded hydrogel exhibited good antioxidant properties in vitro. In a rat model of deep second-degree burn wounds, the SaB-loaded hydrogel could quickly reduce wound temperature, regulate wound oxidant microenvironment, promote angiogenesis, and accelerate wound healing. Thus, the drug-loaded hydrogel shows significant potential as a first-aid dressing for treatment of burn wounds.展开更多
A hydrogel dressing based on bacterial cellulose(BC),which is grafted with quaternary ammonium func-tional and crosslinked with the gelatin-heparin system,is prepared to provide the features mainly con-cerning softnes...A hydrogel dressing based on bacterial cellulose(BC),which is grafted with quaternary ammonium func-tional and crosslinked with the gelatin-heparin system,is prepared to provide the features mainly con-cerning softness,high swelling ratio,antibacterial property,and biocompatibility.An innovation of prepa-ration is that the BC is beaten into short-chain scaffolds to improve the efficiency of grafting,which not only simplifies the preparation process but also avoids the biotoxicity caused by the introduction of toxic catalyst such as dimethyl sulfoxide(DMSO)or uncertain toxic side products in long-chain graft-ing.Scanning electron microscopy(SEM)shows that the QBC/Hep/Gel composite hydrogel possesses a three-dimensional mesh structure with high porosity.The hydrogel shows outstanding water manage-ment performance indicated by the swelling ratio of 1476%,water retention ratio of more than 90%at 120 h,and moisture permeability of 3296 g m^(-2) 24 h^(-1).The antibacterial experiment is implemented with staphylococcus aureus,and the antibacterial effect is represented by an inhibition zone of 3 cm in diameter.In vivo animal experiments suggested that QBC/Hep/Gel could effectively promote epithelial reconstruction,collagen deposition,and angiogenesis in normal wounds,reduce inflammation,and ac-celerate wound healing.All these results indicate that the proposed QBC/Hep/Gel hydrogel is a potential composite for antibacterial dressing.展开更多
Diabetic foot ulcer(DFU)often evolves into chronic wounds that resist healing over an extended period,sometimes necessitating amputation in severe cases.Traditional wound management approaches generally fail to contro...Diabetic foot ulcer(DFU)often evolves into chronic wounds that resist healing over an extended period,sometimes necessitating amputation in severe cases.Traditional wound management approaches generally fail to control these chronic sores successfully.Thus,it arouses a huge demand in clinic for a novel wound dressing to treat DFU effectively.Hydrogel as an ideal delivery system exhibits excellent loading capacity and sustainable release behavior.It also boasts tunable physical and chemical properties adaptable to diverse biomedical scenarios,making it a suitable material for fabricating functional wound dressings to treat DFU.The hydrogel dressings are classified into hemostatic,antibacterial and anti-inflammatory,and healing-promoting hydrogel dressings by associating the pathogenesis of DFU in this paper.The design and fabrication strategies for the dressings,as well as their therapeutic effects in treating DFU,are extensively reviewed.Additionally,this paper highlights future perspectives of multifunctional hydrogel dressings in DFU treatment.This review aims to provide valuable references for material scientists to design and develop hydrogel wound dressings with enhanced capabilities for DFU treatment,and to further translate them intothe clinic inthefuture.展开更多
Bioactive tragacanth gum(TG)was functionalized by covalent crosslinking of poly[2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide(PMEDSAH)to design network structure in form of hydrogel wound dressin...Bioactive tragacanth gum(TG)was functionalized by covalent crosslinking of poly[2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide(PMEDSAH)to design network structure in form of hydrogel wound dressings(HWD).These copolymers were encapsulated with antibiotic drug vancomycin to enhance wound healing potential of the dressings.The copolymers were characterized by SEM,AFM,FTIR,13C NMR,XRD,and TGA-DSC analysis.SEM demonstrated uneven heterogeneous morphology and AFM revealed rough surface of copolymer.Inclusion of synthetic component into HD was confirmed by FTIR and 13C NMR.Hydrogel dressings absorbed 8.491.03 g/g simulated wound fluid and exhibited non-hemolytic(3.70.02%hemolytic potential)and antioxidant(37.421.54%free radical scavenging in DPPH assay)properties.Polymers required 35.05.0 mN detachment force to get it detach from the mucosal surface during mucoadhesive test.Tensile strength was found to be 1.880.13 N/mm2 during mechanical stability test.Hydrogel dressings were permeable to O2/H2O and impermeable to microbes.Release of drug vancomycin occurred through non-Fickian diffusion mechanism and release profile was best described by Korsmeyer-Peppas kinetic model.Overall,these results revealed that these hydrogels could be explored as materials for hydrogel wound dressings.展开更多
Hydrogels are emerging as the most promising dressings due to their excellent biocompatibility,extracellular matrix mimicking structure,and drug loading ability.However,existing hydrogel dressings exhibit limited brea...Hydrogels are emerging as the most promising dressings due to their excellent biocompatibility,extracellular matrix mimicking structure,and drug loading ability.However,existing hydrogel dressings exhibit limited breathability,poor environmental adaptability,potential drug resistance,and limited drug options,which extremely restrict their therapeutic effect and working scenarios.Here,the current research introduces the first paradigm of hydrogel textile dressings based on novel gelatin glycerin hydrogel(glyhydrogel)fibers fabricated by the Hofmeister effect based wet spinning.Benefiting from the unique knitted structure,the textile dressing features excellent breathability(1800 times that of the commercially available 3 M dressing)and stretchability(535.51±38.66%).Furthermore,the glyhydrogel textile dressing can also withstand the extreme temperature of-80℃,showing the potential for application in subzero environments.Moreover,the introduction of glycerin endows the textile dressing with remarkable antibacterial property and expands the selection of loaded drugs(e.g.,clindamycin).The prepared glyhydrogel textile dressing shows an excellent infected wound healing effect with a complete rat skin closure within 14 days.All these functions have not been achievable by traditional hydrogel dressings and provide a new approach for the development of hydrogel dressings.展开更多
Diabetic wounds, as a complication of diabetes, are slow to heal and seriously affect the quality of life of patients. Functional hydrogel dressing is an effective approach to improve diabetic wound healing. Electrica...Diabetic wounds, as a complication of diabetes, are slow to heal and seriously affect the quality of life of patients. Functional hydrogel dressing is an effective approach to improve diabetic wound healing. Electrical stimulation (ES) therapy is conducive to promoting cell migration and wound healing. In this work, a multifunctional PPTZ hydrogel wound dressing was developed by freeze-thaw method with polyvinyl alcohol (PVA), phytic acid (PA), tannic acid (TA), and Zinc chloride. The obtained PPTZ hydrogel has good mechanical properties (stress and strain of 700.03 kPa and 575.08%), light transmittance (close to 100%) and antibacterial rate (over 75%). With good biocompatibility, antioxidant abilities and conductivity, the PPTZ hydrogel could effectively promote the healing of diabetic wounds with two weeks under the action of electric field, which provides an auxiliary treatment strategy for diabetic patients.展开更多
This article deals with the exploration of the design of network crosslinked structure by covalent bonding for use as wound dressing materials keeping in view inherent therapeutic role of neem gum in wound healing.The...This article deals with the exploration of the design of network crosslinked structure by covalent bonding for use as wound dressing materials keeping in view inherent therapeutic role of neem gum in wound healing.These copolymeric hydrogels were prepared by graft-copolymerization reaction of carbopol and poly(N-vinylpyrrolidone)(poly(NVP).Antibiotic drug levofloxacin was encapsulated in dressings for better wound healing.The scanning electron microscopy(SEM),atomic force microscopy(AFM),Fourier transform infrared(FTIR)spectroscopy,^(13)C nuclear magnetic resonance(NMR)spectroscopy,X-ray diffraction(XRD),thermogravimetric analysis(TGA),differential thermal gravimetry(DTG),and differential scanning calorimetry(DSC)techniques were applied for characterization.NMR and FTIR demonstrated incorporation of carbapol and poly(NVP)in dressings.XRD indicated amorphous state in copolymeric dressings.Interactions of copolymers with drug,blood and bio membrane were studied to evaluate biomedical properties which revealed controlled release of drug from hydrogel dressings which were possessing blood-compatible(haemolytic value=2.4±0.82)and mucoadhesive(detachment force=124±41 mN)properties.Dressings were permeable to O2 and H2O vapour and absorbed 6.68±±0.62 g/g wound fluid which is ideal dressing characteristic.Diffusion mechanism type of drug levofloxacin was Fickian with kinetic model First order.These properties suggested use of hydrogel material for wound dressing and drug delivery applications for better wound care management.This article deals with the exploration of the design of network crosslinked structure by covalent bonding for use as wound dressing materials keeping in view inherent therapeutic role of neem gum in wound healing.These copolymeric hydrogels were prepared by graft-copolymerization reaction of carbopol and poly(N-vinylpyrrolidone)(poly(NVP).Antibiotic drug levofloxacin was encapsulated in dressings for better wound healing.The scanning electron microscopy(SEM),atomic force microscopy(AFM),Fourier transform infrared(FTIR)spectroscopy,13C nuclear magnetic resonance(NMR)spectroscopy,X-ray diffraction(XRD),thermogravimetric analysis(TGA),differential thermal gravimetry(DTG),and differential scanning calorimetry(DSC)techniques were applied for characterization.NMR and FTIR demonstrated incorporation of carbapol and poly(NVP)in dressings.XRD indicated amorphous state in copolymeric dressings.Interactions of copolymers with drug,blood and bio membrane were studied to evaluate biomedical properties which revealed controlled release of drug from hydrogel dressings which were possessing blood-compatible(haemolytic value=2.45±.82)and mucoadhesive(detachment force=124±41 mN)properties.Dressings were permeable to O2 and H_(2)O vapour and absorbed 6.68±0.62 g/g wound fluid which is ideal dressing characteristic.Diffusion mechanism type of drug levofloxacin was Fickian with kinetic model First order.These properties suggested use of hydrogel material for wound dressing and drug delivery applications for better wound care management.展开更多
Bacterial infection,excessive inflammation and damaging blood vessels network are the major factors to delay the healing of diabetic ulcer.At present,most of wound repair materials are passive and can’t response to t...Bacterial infection,excessive inflammation and damaging blood vessels network are the major factors to delay the healing of diabetic ulcer.At present,most of wound repair materials are passive and can’t response to the wound microenvironment,resulting in a low utilization of bioactive substances and hence a poor therapeutic effect.Therefore,it’s essential to design an intelligent wound dressing responsive to the wound microenvironment to achieve the release of drugs on-demand on the basis of multifunctionality.In this work,metformin-laden CuPDA NPs composite hydrogel(Met@CuPDA NPs/HG)was fabricated by dynamic phenylborate bonding of gelatin modified by dopamine(Gel-DA),Cu-loaded polydopamine nanoparticles(CuPDA NPs)with hyaluronic acid modified by phenyl boronate acid(HA-PBA),which possessed good injectability,self-healing,adhesive and DPPH scavenging performance.The slow release of metformin was achieved by the interaction with CuPDA NPs,boric groups(B-N coordination)and the constraint of hydrogel network.Metformin had a pH and glucose responsive release behavior to treat different wound microenvironment intelligently.Moreover,CuPDA NPs endowed the hydrogel excellent photothermal responsiveness to kill bacteria of>95%within 10 min and also the slow release of Cu^(2+)to protect wound from infection for a long time.Met@CuPDA NPs/HG also recruited cells to a certain direction and promoted vascularization by releasing Cu^(2+).More importantly,Met@CuPDA NPs/HG effectively decreased the inflammation by eliminating ROS and inhibiting the activation of NF-κB pathway.Animal experiments demonstrated that Met@CuPDA NPs/HG significantly promoted wound healing of diabetic SD rats by killing bacteria,inhibiting inflammation,improving angiogenesis and accelerating the deposition of ECM and collagen.Therefore,Met@CuPDA NPs/HG had a great application potential for diabetic wound healing.展开更多
Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has crea...Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has created a new problem of superbugs.Herein,we propose a new strategy to obtain pH-responsive antimicrobial P-ZIF(ZIF:zeolitic imidazolate framework)by loading polyhexamethylenebiguanide(PHMB)into the framework of ZIF-8 nanoparticles.This will enable PHMB to be released in the weak acid environment of an infected wound.To address burn infections,P-ZIF nanoparticles were loaded into a hydrogel system made of sodium alginate(SA)and 3-aminophenylboronic acid modified human-like collagen(H-A)through borate ester bonds.The resulting H-A/SA/P-ZIF(HASPZ)hydrogel dressing not only possesses antibacterial and wound healing properties but also has dual pH responsiveness to prevent the overuse of medication while effectively treat deep second-degree burns.Therefore,P-ZIF nanoparticles and the corresponding HASPZ hydrogel dressing are considered of significant importance in antimicrobial,drug delivery,and wound repair.展开更多
Excessive reactive oxygen species(ROS)at severe burn injury sites may promote metabolic reprogramming of macrophages to induce a deteriorative and uncontrolled inflammation cycle,leading to delayed wound healing and r...Excessive reactive oxygen species(ROS)at severe burn injury sites may promote metabolic reprogramming of macrophages to induce a deteriorative and uncontrolled inflammation cycle,leading to delayed wound healing and regeneration.Here,a novel bioactive,anti-fouling,flexible polyzwitterionic hydrogel encapsulated with epigallocatechin gallate(EGCG)-copper(Cu)capsules(termed as EGCG-Cu@CBgel)is engineered for burn wound management,which is dedicated to synergistically exerting ROS-scavenging,immune metabolic regulation and pro-angiogenic effects.EGCG-Cu@CBgel can scavenge ROS to normalize intracellular redox homeostasis,effectively relieving oxidative damages and blocking proinflammatory signal transduction.Importantly,EGCG-Cu can inhibit the activity of hexokinase and phosphofructokinase,alleviate accumulation of pyruvate and convert it to acetyl coenzyme A(CoA),whereby inhibits glycolysis and normalizes tricarboxylic acid(TCA)cycle.Additionally,metabolic reprogramming of macrophages by EGCG-Cu downregulates M1-type polarization and the expression of proinflammatory cytokines both in vitro and in vivo.Meanwhile,copper ions(Cu^(2+))released from the hydrogel facilitate angiogenesis.EGCG-Cu@CBgel significantly accelerates the healing of severe burn wound via promoting wound closure,weakening tissue-damaging inflammatory responses and enhancing the remodeling of pathological structure.Overall,this study demonstrates the great potential of bioactive hydrogel dressing in treating burn wounds without unnecessary secondary damage to newly formed skin,and highlights the importance of immunometabolism modulation in tissue repair and regeneration.展开更多
Hydrogels are classical soft and wet materials that have been extensively studied over the past several decades. Recently, with the development of supramolecular science, nanotechnology and precisely synthetic chemist...Hydrogels are classical soft and wet materials that have been extensively studied over the past several decades. Recently, with the development of supramolecular science, nanotechnology and precisely synthetic chemistry, various novel hydrogels have been designed and fabricated, which show emerging applications in tissue engineering, drug delivery, anti-fouling coatings, flexible electronics and soft robotics. Through tailoring their two-dimensional surface structures and three- dimensional networks, unique properties such as ultra-high mechanical strength, responsiveness to various kinds of stimuli, biocompatibility, special wettability and adhesion can be achieved.展开更多
基金supported by the Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(2021SZ-TD004)the Natural Science Foundation of Zhejiang Province(LD21E030001)+1 种基金the Joint Fund of National Natural Science Foundation of China(U22A20155)the Lingyan Program of Zhejiang Province(2022C01106).
文摘Wound management is an important issue that places enormous pressure on the physical and mental health of patients,especially in cases of infection,where the increased inflammatory response could lead to severe hypertrophic scars(HSs).In this study,a hydrogel dressing was developed by combining the high strength and toughness,swelling resistance,antibacterial and antioxidant capabilities.The hydrogel matrix was composed of a double network of polyvinyl alcohol(PVA)and agarose with excellent mechanical properties.Hyperbranched polylysine(HBPL),a highly effective antibacterial cationic polymer,and tannic acid(TA),a strong antioxidant molecule,were added to the hydrogel as functional components.Examination of antibacterial and antioxidant properties of the hydrogel confirmed the full play of the efficacy of HBPL and TA.In the in vivo studies of methicillin-resistant Staphylococcus aureus(MRSA)infection,the hydrogel had shown obvious promotion of wound healing,and more profoundly,significant suppression of scar formation.Due to the common raw materials and simple preparation methods,this hydrogel can be mass produced and used for accelerating wound healing while preventing HSs in infected wounds.
基金supported by the National Natural Science Foundation of China(12272253,82103147)Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(2021SX-AT008,2021SX-AT009)+1 种基金the Foundamental Research Program of Shanxi Province,China(20210302124405,20210302124082)the Science and Technology projects from Guangzhou City(202102010481).
文摘Rapid post-wound closure is necessary to avoid wound infection and promote scar-free healing when skin trauma occurs.In this study,new types of hydrogel dressings with adjustable contractility were fabricated based on N-isopropyl acrylamide/sodium alginate/graphene oxide(P/SA/GO).Then,the chitosan(CS)solution was used as a bridging polymer to achieve tissue adhesion to the hydrogel.The results show that the hydrogel based on poly(N-isopropyl acrylamide)(PNIPAM)not only has the ability to self-shrink but also can adjust the rate of shrinkage through near-infrared thermal stimulation.At the same time,high adhesion strength(7.86±1.22 kPa)between the tissue and the dressing is achieved through the introduction of bridging polymers(CS),and the coating area of the bridging polymer can be adjusted to achieve regional adhesion.The mouse total skin defects experiments have shown that sutures-free wound closure in the early stages of wound healing could be obtained by adjusting the material temperature.Besides,the dressings can promote scar-free wound healing by reducing inflammatory cell infiltration and collagen deposition.These results indicate that double-crosslinked PNIPAM-based hydrogel dressings with adjustable adhesion and contractility proposed in this study provide a candidate material for achieving trackless wound healing.
基金supported by the National Natural Science Foundation of China(Grant Nos. 52173147, 22105198, 51973218, 51833010)the Scientific and Technological Development Projects of Jilin Province(Grant No. 20210204136YY)。
文摘Burn wounds are destructive skin traumas typically of irregular shape and large area. Prone to infection, they require frequent dressing replacement, and painless removal of dressings from burn wounds remains a major challenge. This study focuses on the dynamic characteristics and treatment difficulty of burn wounds. Hydrogel dressings based on glycol chitosan and propionaldehyde-or benzaldehyde-terminated 4-arm poly(ethylene glycol) were designed on the basis of Schiff base cross-linking networks. The hydrogels exhibited shape-adaptability, self-healing and fast-degradation properties, which makes these hydrogels suitable for burn wounds. Salvianolic acid B(SaB)-loaded hydrogel exhibited good antioxidant properties in vitro. In a rat model of deep second-degree burn wounds, the SaB-loaded hydrogel could quickly reduce wound temperature, regulate wound oxidant microenvironment, promote angiogenesis, and accelerate wound healing. Thus, the drug-loaded hydrogel shows significant potential as a first-aid dressing for treatment of burn wounds.
基金Science and Technology Program of Shaanxi Province(No.2019GY-200).
文摘A hydrogel dressing based on bacterial cellulose(BC),which is grafted with quaternary ammonium func-tional and crosslinked with the gelatin-heparin system,is prepared to provide the features mainly con-cerning softness,high swelling ratio,antibacterial property,and biocompatibility.An innovation of prepa-ration is that the BC is beaten into short-chain scaffolds to improve the efficiency of grafting,which not only simplifies the preparation process but also avoids the biotoxicity caused by the introduction of toxic catalyst such as dimethyl sulfoxide(DMSO)or uncertain toxic side products in long-chain graft-ing.Scanning electron microscopy(SEM)shows that the QBC/Hep/Gel composite hydrogel possesses a three-dimensional mesh structure with high porosity.The hydrogel shows outstanding water manage-ment performance indicated by the swelling ratio of 1476%,water retention ratio of more than 90%at 120 h,and moisture permeability of 3296 g m^(-2) 24 h^(-1).The antibacterial experiment is implemented with staphylococcus aureus,and the antibacterial effect is represented by an inhibition zone of 3 cm in diameter.In vivo animal experiments suggested that QBC/Hep/Gel could effectively promote epithelial reconstruction,collagen deposition,and angiogenesis in normal wounds,reduce inflammation,and ac-celerate wound healing.All these results indicate that the proposed QBC/Hep/Gel hydrogel is a potential composite for antibacterial dressing.
基金funded by the Science and Technology Innovation Project,China Academy of Chinese Medical Sciences,clinical study on the effect of modified Bu yang Huan wu decoction combined with early anticoagulant intervention on the prevention of lower extremity deep vein thrombosis syndrome(CI2021A01905)。
文摘Diabetic foot ulcer(DFU)often evolves into chronic wounds that resist healing over an extended period,sometimes necessitating amputation in severe cases.Traditional wound management approaches generally fail to control these chronic sores successfully.Thus,it arouses a huge demand in clinic for a novel wound dressing to treat DFU effectively.Hydrogel as an ideal delivery system exhibits excellent loading capacity and sustainable release behavior.It also boasts tunable physical and chemical properties adaptable to diverse biomedical scenarios,making it a suitable material for fabricating functional wound dressings to treat DFU.The hydrogel dressings are classified into hemostatic,antibacterial and anti-inflammatory,and healing-promoting hydrogel dressings by associating the pathogenesis of DFU in this paper.The design and fabrication strategies for the dressings,as well as their therapeutic effects in treating DFU,are extensively reviewed.Additionally,this paper highlights future perspectives of multifunctional hydrogel dressings in DFU treatment.This review aims to provide valuable references for material scientists to design and develop hydrogel wound dressings with enhanced capabilities for DFU treatment,and to further translate them intothe clinic inthefuture.
文摘Bioactive tragacanth gum(TG)was functionalized by covalent crosslinking of poly[2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide(PMEDSAH)to design network structure in form of hydrogel wound dressings(HWD).These copolymers were encapsulated with antibiotic drug vancomycin to enhance wound healing potential of the dressings.The copolymers were characterized by SEM,AFM,FTIR,13C NMR,XRD,and TGA-DSC analysis.SEM demonstrated uneven heterogeneous morphology and AFM revealed rough surface of copolymer.Inclusion of synthetic component into HD was confirmed by FTIR and 13C NMR.Hydrogel dressings absorbed 8.491.03 g/g simulated wound fluid and exhibited non-hemolytic(3.70.02%hemolytic potential)and antioxidant(37.421.54%free radical scavenging in DPPH assay)properties.Polymers required 35.05.0 mN detachment force to get it detach from the mucosal surface during mucoadhesive test.Tensile strength was found to be 1.880.13 N/mm2 during mechanical stability test.Hydrogel dressings were permeable to O2/H2O and impermeable to microbes.Release of drug vancomycin occurred through non-Fickian diffusion mechanism and release profile was best described by Korsmeyer-Peppas kinetic model.Overall,these results revealed that these hydrogels could be explored as materials for hydrogel wound dressings.
基金National Key Research and Development Program of China(2021YFC2101800,2021YFC2400802)National Natural Science Foundation of China(52173117)+5 种基金Natural Science Foundation of Shanghai(20ZR1402500)Belt&Road Young Scientist Exchanges Project of Science and Technology Commission Foundation of Shanghai(20520741000)Ningbo 2025 Science and Technology Major Project(2019B10068)Science and Technology Commission of Shanghai Municipality(20DZ2254900,20DZ2270800)Fundamental Research Funds for the Central Universities,DHU Distinguished Young Professor Program(LZA2019001)Shanghai Stomatological Hospital Science and Technology Talents Project(SSH-2022-KJCX-B01).
文摘Hydrogels are emerging as the most promising dressings due to their excellent biocompatibility,extracellular matrix mimicking structure,and drug loading ability.However,existing hydrogel dressings exhibit limited breathability,poor environmental adaptability,potential drug resistance,and limited drug options,which extremely restrict their therapeutic effect and working scenarios.Here,the current research introduces the first paradigm of hydrogel textile dressings based on novel gelatin glycerin hydrogel(glyhydrogel)fibers fabricated by the Hofmeister effect based wet spinning.Benefiting from the unique knitted structure,the textile dressing features excellent breathability(1800 times that of the commercially available 3 M dressing)and stretchability(535.51±38.66%).Furthermore,the glyhydrogel textile dressing can also withstand the extreme temperature of-80℃,showing the potential for application in subzero environments.Moreover,the introduction of glycerin endows the textile dressing with remarkable antibacterial property and expands the selection of loaded drugs(e.g.,clindamycin).The prepared glyhydrogel textile dressing shows an excellent infected wound healing effect with a complete rat skin closure within 14 days.All these functions have not been achievable by traditional hydrogel dressings and provide a new approach for the development of hydrogel dressings.
基金supported by the National key Research and development program of China(No.2022YFC2106100)the National Natural Science Foundation of China(Nos.22078265,and 21908179)the Natural Science Foundation of Shaanxi Province,China(No.218JQ2052).
文摘Diabetic wounds, as a complication of diabetes, are slow to heal and seriously affect the quality of life of patients. Functional hydrogel dressing is an effective approach to improve diabetic wound healing. Electrical stimulation (ES) therapy is conducive to promoting cell migration and wound healing. In this work, a multifunctional PPTZ hydrogel wound dressing was developed by freeze-thaw method with polyvinyl alcohol (PVA), phytic acid (PA), tannic acid (TA), and Zinc chloride. The obtained PPTZ hydrogel has good mechanical properties (stress and strain of 700.03 kPa and 575.08%), light transmittance (close to 100%) and antibacterial rate (over 75%). With good biocompatibility, antioxidant abilities and conductivity, the PPTZ hydrogel could effectively promote the healing of diabetic wounds with two weeks under the action of electric field, which provides an auxiliary treatment strategy for diabetic patients.
文摘This article deals with the exploration of the design of network crosslinked structure by covalent bonding for use as wound dressing materials keeping in view inherent therapeutic role of neem gum in wound healing.These copolymeric hydrogels were prepared by graft-copolymerization reaction of carbopol and poly(N-vinylpyrrolidone)(poly(NVP).Antibiotic drug levofloxacin was encapsulated in dressings for better wound healing.The scanning electron microscopy(SEM),atomic force microscopy(AFM),Fourier transform infrared(FTIR)spectroscopy,^(13)C nuclear magnetic resonance(NMR)spectroscopy,X-ray diffraction(XRD),thermogravimetric analysis(TGA),differential thermal gravimetry(DTG),and differential scanning calorimetry(DSC)techniques were applied for characterization.NMR and FTIR demonstrated incorporation of carbapol and poly(NVP)in dressings.XRD indicated amorphous state in copolymeric dressings.Interactions of copolymers with drug,blood and bio membrane were studied to evaluate biomedical properties which revealed controlled release of drug from hydrogel dressings which were possessing blood-compatible(haemolytic value=2.4±0.82)and mucoadhesive(detachment force=124±41 mN)properties.Dressings were permeable to O2 and H2O vapour and absorbed 6.68±±0.62 g/g wound fluid which is ideal dressing characteristic.Diffusion mechanism type of drug levofloxacin was Fickian with kinetic model First order.These properties suggested use of hydrogel material for wound dressing and drug delivery applications for better wound care management.This article deals with the exploration of the design of network crosslinked structure by covalent bonding for use as wound dressing materials keeping in view inherent therapeutic role of neem gum in wound healing.These copolymeric hydrogels were prepared by graft-copolymerization reaction of carbopol and poly(N-vinylpyrrolidone)(poly(NVP).Antibiotic drug levofloxacin was encapsulated in dressings for better wound healing.The scanning electron microscopy(SEM),atomic force microscopy(AFM),Fourier transform infrared(FTIR)spectroscopy,13C nuclear magnetic resonance(NMR)spectroscopy,X-ray diffraction(XRD),thermogravimetric analysis(TGA),differential thermal gravimetry(DTG),and differential scanning calorimetry(DSC)techniques were applied for characterization.NMR and FTIR demonstrated incorporation of carbapol and poly(NVP)in dressings.XRD indicated amorphous state in copolymeric dressings.Interactions of copolymers with drug,blood and bio membrane were studied to evaluate biomedical properties which revealed controlled release of drug from hydrogel dressings which were possessing blood-compatible(haemolytic value=2.45±.82)and mucoadhesive(detachment force=124±41 mN)properties.Dressings were permeable to O2 and H_(2)O vapour and absorbed 6.68±0.62 g/g wound fluid which is ideal dressing characteristic.Diffusion mechanism type of drug levofloxacin was Fickian with kinetic model First order.These properties suggested use of hydrogel material for wound dressing and drug delivery applications for better wound care management.
基金supported by the National Natural Science Foundation of China(Grant No.52272276,52073103,52203164)the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B090924004)+2 种基金the funds for Zhongshan Innovation Project of high-end Scientific Research Institutions(Grant No.2020AG020)the Fundamental Research Funds for the Central Universities(No.2022ZYGXZR105)Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515010905).
文摘Bacterial infection,excessive inflammation and damaging blood vessels network are the major factors to delay the healing of diabetic ulcer.At present,most of wound repair materials are passive and can’t response to the wound microenvironment,resulting in a low utilization of bioactive substances and hence a poor therapeutic effect.Therefore,it’s essential to design an intelligent wound dressing responsive to the wound microenvironment to achieve the release of drugs on-demand on the basis of multifunctionality.In this work,metformin-laden CuPDA NPs composite hydrogel(Met@CuPDA NPs/HG)was fabricated by dynamic phenylborate bonding of gelatin modified by dopamine(Gel-DA),Cu-loaded polydopamine nanoparticles(CuPDA NPs)with hyaluronic acid modified by phenyl boronate acid(HA-PBA),which possessed good injectability,self-healing,adhesive and DPPH scavenging performance.The slow release of metformin was achieved by the interaction with CuPDA NPs,boric groups(B-N coordination)and the constraint of hydrogel network.Metformin had a pH and glucose responsive release behavior to treat different wound microenvironment intelligently.Moreover,CuPDA NPs endowed the hydrogel excellent photothermal responsiveness to kill bacteria of>95%within 10 min and also the slow release of Cu^(2+)to protect wound from infection for a long time.Met@CuPDA NPs/HG also recruited cells to a certain direction and promoted vascularization by releasing Cu^(2+).More importantly,Met@CuPDA NPs/HG effectively decreased the inflammation by eliminating ROS and inhibiting the activation of NF-κB pathway.Animal experiments demonstrated that Met@CuPDA NPs/HG significantly promoted wound healing of diabetic SD rats by killing bacteria,inhibiting inflammation,improving angiogenesis and accelerating the deposition of ECM and collagen.Therefore,Met@CuPDA NPs/HG had a great application potential for diabetic wound healing.
基金supported by the National key Research and Development Program of China(Nos.2021YFC2101504,2021YFC2103900,and 2019YFA0905200)the National Natural Science Foundation of China(Nos.22078265 and 21908179)the Natural Science Foundation of Shaanxi Province,China(No.218JQ2052).
文摘Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has created a new problem of superbugs.Herein,we propose a new strategy to obtain pH-responsive antimicrobial P-ZIF(ZIF:zeolitic imidazolate framework)by loading polyhexamethylenebiguanide(PHMB)into the framework of ZIF-8 nanoparticles.This will enable PHMB to be released in the weak acid environment of an infected wound.To address burn infections,P-ZIF nanoparticles were loaded into a hydrogel system made of sodium alginate(SA)and 3-aminophenylboronic acid modified human-like collagen(H-A)through borate ester bonds.The resulting H-A/SA/P-ZIF(HASPZ)hydrogel dressing not only possesses antibacterial and wound healing properties but also has dual pH responsiveness to prevent the overuse of medication while effectively treat deep second-degree burns.Therefore,P-ZIF nanoparticles and the corresponding HASPZ hydrogel dressing are considered of significant importance in antimicrobial,drug delivery,and wound repair.
基金the National Natural Science Foundation of China(No.82272162,82001954)Fundamental Research Funds for the Central Universities (3332021067, 2019PT350005)+2 种基金Natural Science Fund for Distinguished Young Scholars of Tianjin (21JCJQJC00020)CAMS Innovation Fund for Medical Sciences (2021-I2M-1-052, 2021- I2M-1-058, 2021-I2M-1-065)Tianjin Innovation and Promotion Plan Key Innovation Team of Implantable and Interventional Biomedical Materials.
文摘Excessive reactive oxygen species(ROS)at severe burn injury sites may promote metabolic reprogramming of macrophages to induce a deteriorative and uncontrolled inflammation cycle,leading to delayed wound healing and regeneration.Here,a novel bioactive,anti-fouling,flexible polyzwitterionic hydrogel encapsulated with epigallocatechin gallate(EGCG)-copper(Cu)capsules(termed as EGCG-Cu@CBgel)is engineered for burn wound management,which is dedicated to synergistically exerting ROS-scavenging,immune metabolic regulation and pro-angiogenic effects.EGCG-Cu@CBgel can scavenge ROS to normalize intracellular redox homeostasis,effectively relieving oxidative damages and blocking proinflammatory signal transduction.Importantly,EGCG-Cu can inhibit the activity of hexokinase and phosphofructokinase,alleviate accumulation of pyruvate and convert it to acetyl coenzyme A(CoA),whereby inhibits glycolysis and normalizes tricarboxylic acid(TCA)cycle.Additionally,metabolic reprogramming of macrophages by EGCG-Cu downregulates M1-type polarization and the expression of proinflammatory cytokines both in vitro and in vivo.Meanwhile,copper ions(Cu^(2+))released from the hydrogel facilitate angiogenesis.EGCG-Cu@CBgel significantly accelerates the healing of severe burn wound via promoting wound closure,weakening tissue-damaging inflammatory responses and enhancing the remodeling of pathological structure.Overall,this study demonstrates the great potential of bioactive hydrogel dressing in treating burn wounds without unnecessary secondary damage to newly formed skin,and highlights the importance of immunometabolism modulation in tissue repair and regeneration.
文摘Hydrogels are classical soft and wet materials that have been extensively studied over the past several decades. Recently, with the development of supramolecular science, nanotechnology and precisely synthetic chemistry, various novel hydrogels have been designed and fabricated, which show emerging applications in tissue engineering, drug delivery, anti-fouling coatings, flexible electronics and soft robotics. Through tailoring their two-dimensional surface structures and three- dimensional networks, unique properties such as ultra-high mechanical strength, responsiveness to various kinds of stimuli, biocompatibility, special wettability and adhesion can be achieved.
