<strong>Objective: </strong><span style="font-family:""><span style="font-family:Verdana;">To investigate the clinical effects of</span><a name="_Hlk26140...<strong>Objective: </strong><span style="font-family:""><span style="font-family:Verdana;">To investigate the clinical effects of</span><a name="_Hlk26140736"></a><span style="font-family:Verdana;"> recombinant human interleukin-2 (rhIL-2) combined with </span><a name="_Hlk26140744"></a><span style="font-family:Verdana;">Zhenqi Fuzheng and Baofukang on cervical intraepithelial neoplasia II (CINII) combined with human papilloma virus infection. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">There were 593 patients diagnosed with CINII with HPV infection, including 296 in the control group and 297 in the experimental group. The control group was given only Zhenqi Fuzheng oral and Baofukang suppository vaginal medicine. The experimental group was treated with rhIL-2 injection in addition to Zhenqi Fuzheng oral and Baofukang suppository vaginal medicine which is treated for 3 months. After 3 months, Thinprep cytologic test (TCT), human papilloma virus (HPV) quantitative examination and colposcopy biopsy were reviewed. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> After 3 months of treatment, the negative conversion rate and total effective rate of HPV in the control group were 58.11% and 70.95% respectively, and the negative conversion rate and total effective rate of the experimental group were 79.46% and 90.57% respectively. There was significant difference between the two groups (p = 0.000). The curative rate of cervical lesions was significantly higher in the test group than in the control group, 89.56%, 68.91%, respectively. The statistical difference between the two groups is significant (p = 0.000). </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> It has an essential clinical value that HPV infection patients and cervical intraepithelial neoplasia II associated with </span><a name="_Hlk47768779"></a><span style="font-family:Verdana;">HPV infection patients are treated by rhIL-2 combined with Zhenqifuzheng and Baofukang, </span><a name="_Hlk47805707"></a><span style="font-family:Verdana;">which is safe, effective, non-invasive, reusable advantages. However, the long-term efficacy and side effects need to be further studied.</span></span>展开更多
Purpose: Bursal inflammation is thought to be a major cause of pain in degenerative rotator cuff tears (RCTs). While the expression of proinflammatory mediators, such as COX-2, TNF-α, IL-1β, and IL-6, is crucial for...Purpose: Bursal inflammation is thought to be a major cause of pain in degenerative rotator cuff tears (RCTs). While the expression of proinflammatory mediators, such as COX-2, TNF-α, IL-1β, and IL-6, is crucial for the pathophysiology of osteoarthritis (OA), their role in degenerative RCTs remains unknown. The aim of this study was to determine the expression of COX-2 and proinflammatory mediators in the development of RCT-induced pain by comparing their levels in patients with hip OA or RCTs. Methods: We included samples obtained from 31 shoulders of 31 patients with RCTs and samples from 30 hips of 27 patients with hip OA. The mRNA levels of COX-2, TNF-α, IL-1β, and IL-6 were determined using RT-PCR, and were compared between the subacromial bursa and hip joints. We also analyzed IL-1β-induced COX-2 expression in the subacromial bursa and synovial blast of the hip. Results: COX-2, IL-1β, and IL-6 expression levels were significantly lower in the subacromial bursa of RCTs than in hip OA samples, while no significant difference was observed for TNF-α. No significant difference in the fold increase was observed between subacromial bursa and hip OA samples, even though IL-1β-induced COX-2 expression increased in both samples. Conclusion: Our findings suggest that the main mechanism underlying pain development differs between patients with RCTs and those with hip OA.展开更多
Steroid resistance represents a major clinical problem in the treatment of severe asthma,and therefore a better understanding of its pathogenesis is warranted.Recent studies indicated that histone deacetylase 2(HDAC2)...Steroid resistance represents a major clinical problem in the treatment of severe asthma,and therefore a better understanding of its pathogenesis is warranted.Recent studies indicated that histone deacetylase 2(HDAC2)and interleukin 17A(IL-17A)play important roles in severe asthma.HDAC2 activity is reduced in patients with severe asthma and smoking-induced asthma,perhaps accounting for the amplified expression of inflammatory genes,which is associated with increased acetylation of glucocorticoid receptors.Neutrophilic inflammation contributes to severe asthma and may be related to T helper(Th)17 rather than Th2 cytokines.IL-17A levels are elevated in severe asthma and correlate with the presence of neutrophils.Restoring the activity of HDAC2 or targeting the Th17 signaling pathway is a potential therapeutic approach to reverse steroid insensitivity.展开更多
Objective To investigate wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor.Method Group experiment and statistical analysis were conducted.Results were analyzed comparative...Objective To investigate wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor.Method Group experiment and statistical analysis were conducted.Results were analyzed comparatively.Result Wheat maxibastion of Guanyuan point could reduce ATEP(associated tumor erythrocyte rate) and direct tumor erythrocyte rate(DTER) and raise excitotumorous erythrocyte rate(ETER)(P< 0.05,P< 0.01) and stimulate the reduced activity of NK cells and IL 2 significantly(P< 0.01) in mice with tumor.Conclusion Wheat maxibastion of Guanyuan point can increase the adhesion ability to tumor cells of RBC,and increase the activity of NK cell and IL 2.展开更多
Objective To investigate the antigen-specific T cell functionality in type 2 diabetes mellitus patients.Methods Peripheral blood from 38 type 2 diabetes mellitus patients and 47 healthy controls(control group)were col...Objective To investigate the antigen-specific T cell functionality in type 2 diabetes mellitus patients.Methods Peripheral blood from 38 type 2 diabetes mellitus patients and 47 healthy controls(control group)were collected.The proportions of CD4^+and CD8^+ T cell as well as the ratio of CD4+/CD8 + were monitored by flow cytometry.Meanwhile,antigen-nonspecific and specific Th1 responses were compared between the two groups through detecting interferon(IFN)-γ,interleukin 2(IL-2),and tumor necrosis factor(TNF)-α producing cells upon propylene glycol monomethyl ether acetate(PMA)/ionomycine and epstein-barr virus(EBV)peptides stimulation,respectively followed by an intracellular cytokine staining.Results Compared to the control group,the proportion of CD4^+T cell and the ratio of CD4^+/CD8^+ were significantly increased in the type 2 diabetes mellitus group(P<0.05)whereas CD8+T cells exhibited no significant difference between the two groups.Antigen-nonspecific Th1 responses in type 2 diabetes mellitus patients were significantly decreased,demonstrated by lower percentages of IFN-γ,IL-2,and TNF-α producing CD4^+T cells when compared to the control group,while CD8^+T cells in the type 2 diabetes mellitus patients exhibited similar cytokine production patterns.However,when stimulated by EBV specific peptides,the percentages of IFN-γ,IL-2,and TNF-α producing CD8^+T cells were significantly higher in type 2 diabetes mellitus patients than those in the control group(P<0.05).Hb A1 C was positively correlated with the percentage of EBV-specific TNF-α producing CD8^+T cells(P<0.05).Conclusion In type 2 diabetes mellitus,the secretion capacity of CD4^+and CD8^+ T cell was significantly decreased and the antigen-specific responses represent the presence of an abnormal activated status,which indicates that chronic hyperglycemia may damage T cells function and aggravate chronic inflammation.展开更多
文摘<strong>Objective: </strong><span style="font-family:""><span style="font-family:Verdana;">To investigate the clinical effects of</span><a name="_Hlk26140736"></a><span style="font-family:Verdana;"> recombinant human interleukin-2 (rhIL-2) combined with </span><a name="_Hlk26140744"></a><span style="font-family:Verdana;">Zhenqi Fuzheng and Baofukang on cervical intraepithelial neoplasia II (CINII) combined with human papilloma virus infection. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">There were 593 patients diagnosed with CINII with HPV infection, including 296 in the control group and 297 in the experimental group. The control group was given only Zhenqi Fuzheng oral and Baofukang suppository vaginal medicine. The experimental group was treated with rhIL-2 injection in addition to Zhenqi Fuzheng oral and Baofukang suppository vaginal medicine which is treated for 3 months. After 3 months, Thinprep cytologic test (TCT), human papilloma virus (HPV) quantitative examination and colposcopy biopsy were reviewed. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> After 3 months of treatment, the negative conversion rate and total effective rate of HPV in the control group were 58.11% and 70.95% respectively, and the negative conversion rate and total effective rate of the experimental group were 79.46% and 90.57% respectively. There was significant difference between the two groups (p = 0.000). The curative rate of cervical lesions was significantly higher in the test group than in the control group, 89.56%, 68.91%, respectively. The statistical difference between the two groups is significant (p = 0.000). </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> It has an essential clinical value that HPV infection patients and cervical intraepithelial neoplasia II associated with </span><a name="_Hlk47768779"></a><span style="font-family:Verdana;">HPV infection patients are treated by rhIL-2 combined with Zhenqifuzheng and Baofukang, </span><a name="_Hlk47805707"></a><span style="font-family:Verdana;">which is safe, effective, non-invasive, reusable advantages. However, the long-term efficacy and side effects need to be further studied.</span></span>
文摘Purpose: Bursal inflammation is thought to be a major cause of pain in degenerative rotator cuff tears (RCTs). While the expression of proinflammatory mediators, such as COX-2, TNF-α, IL-1β, and IL-6, is crucial for the pathophysiology of osteoarthritis (OA), their role in degenerative RCTs remains unknown. The aim of this study was to determine the expression of COX-2 and proinflammatory mediators in the development of RCT-induced pain by comparing their levels in patients with hip OA or RCTs. Methods: We included samples obtained from 31 shoulders of 31 patients with RCTs and samples from 30 hips of 27 patients with hip OA. The mRNA levels of COX-2, TNF-α, IL-1β, and IL-6 were determined using RT-PCR, and were compared between the subacromial bursa and hip joints. We also analyzed IL-1β-induced COX-2 expression in the subacromial bursa and synovial blast of the hip. Results: COX-2, IL-1β, and IL-6 expression levels were significantly lower in the subacromial bursa of RCTs than in hip OA samples, while no significant difference was observed for TNF-α. No significant difference in the fold increase was observed between subacromial bursa and hip OA samples, even though IL-1β-induced COX-2 expression increased in both samples. Conclusion: Our findings suggest that the main mechanism underlying pain development differs between patients with RCTs and those with hip OA.
基金This work was supported by the Guangdong Basic and Applied Ba-sic Research Foundation(No.2020B1515020004)the National Natural Science Foundation of China(No.81873404)+1 种基金Project of Young Inno-vative Talents in Colleges and Universities in Guangdong Province(No.2018KQNCX095)Affiliated Hospital of Guangdong Medical University Clinical Research Program(Nos.LCYJ2018C001,LCYJ2019B011).
文摘Steroid resistance represents a major clinical problem in the treatment of severe asthma,and therefore a better understanding of its pathogenesis is warranted.Recent studies indicated that histone deacetylase 2(HDAC2)and interleukin 17A(IL-17A)play important roles in severe asthma.HDAC2 activity is reduced in patients with severe asthma and smoking-induced asthma,perhaps accounting for the amplified expression of inflammatory genes,which is associated with increased acetylation of glucocorticoid receptors.Neutrophilic inflammation contributes to severe asthma and may be related to T helper(Th)17 rather than Th2 cytokines.IL-17A levels are elevated in severe asthma and correlate with the presence of neutrophils.Restoring the activity of HDAC2 or targeting the Th17 signaling pathway is a potential therapeutic approach to reverse steroid insensitivity.
文摘Objective To investigate wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor.Method Group experiment and statistical analysis were conducted.Results were analyzed comparatively.Result Wheat maxibastion of Guanyuan point could reduce ATEP(associated tumor erythrocyte rate) and direct tumor erythrocyte rate(DTER) and raise excitotumorous erythrocyte rate(ETER)(P< 0.05,P< 0.01) and stimulate the reduced activity of NK cells and IL 2 significantly(P< 0.01) in mice with tumor.Conclusion Wheat maxibastion of Guanyuan point can increase the adhesion ability to tumor cells of RBC,and increase the activity of NK cell and IL 2.
文摘Objective To investigate the antigen-specific T cell functionality in type 2 diabetes mellitus patients.Methods Peripheral blood from 38 type 2 diabetes mellitus patients and 47 healthy controls(control group)were collected.The proportions of CD4^+and CD8^+ T cell as well as the ratio of CD4+/CD8 + were monitored by flow cytometry.Meanwhile,antigen-nonspecific and specific Th1 responses were compared between the two groups through detecting interferon(IFN)-γ,interleukin 2(IL-2),and tumor necrosis factor(TNF)-α producing cells upon propylene glycol monomethyl ether acetate(PMA)/ionomycine and epstein-barr virus(EBV)peptides stimulation,respectively followed by an intracellular cytokine staining.Results Compared to the control group,the proportion of CD4^+T cell and the ratio of CD4^+/CD8^+ were significantly increased in the type 2 diabetes mellitus group(P<0.05)whereas CD8+T cells exhibited no significant difference between the two groups.Antigen-nonspecific Th1 responses in type 2 diabetes mellitus patients were significantly decreased,demonstrated by lower percentages of IFN-γ,IL-2,and TNF-α producing CD4^+T cells when compared to the control group,while CD8^+T cells in the type 2 diabetes mellitus patients exhibited similar cytokine production patterns.However,when stimulated by EBV specific peptides,the percentages of IFN-γ,IL-2,and TNF-α producing CD8^+T cells were significantly higher in type 2 diabetes mellitus patients than those in the control group(P<0.05).Hb A1 C was positively correlated with the percentage of EBV-specific TNF-α producing CD8^+T cells(P<0.05).Conclusion In type 2 diabetes mellitus,the secretion capacity of CD4^+and CD8^+ T cell was significantly decreased and the antigen-specific responses represent the presence of an abnormal activated status,which indicates that chronic hyperglycemia may damage T cells function and aggravate chronic inflammation.
