Lipid peroxidation and iron accumulation are closely associated with neurodegenerative diseases,such as Alzheimer’s,Parkinson’s,and Huntington’s diseases,or neurodegeneration with brain iron accumulation disorders....Lipid peroxidation and iron accumulation are closely associated with neurodegenerative diseases,such as Alzheimer’s,Parkinson’s,and Huntington’s diseases,or neurodegeneration with brain iron accumulation disorders.Mitochondrial dysfunction,lipofuscin accumulation,autophagy disruption,and ferroptosis have been implicated as the critical pathomechanisms of lipid peroxidation and iron accumulation in these disorders.Currently,the connection between lipid peroxidation and iron accumulation and the initial cause or consequence in neurodegeneration processes is unclear.In this review,we have compiled the known mechanisms by which lipid peroxidation triggers iron accumulation and lipofuscin formation,and the effect of iron overload on lipid peroxidation and cellular function.The vicious cycle established between both pathological alterations may lead to the development of neurodegeneration.Therefore,the investigation of these mechanisms is essential for exploring therapeutic strategies to restrict neurodegeneration.In addition,we discuss the interplay between lipid peroxidation and iron accumulation in neurodegeneration,particularly in PLA2G6-associated neurodegeneration,a rare neurodegenerative disease with autosomal recessive inheritance,which belongs to the group of neurodegeneration with brain iron accumulation disorders.展开更多
In the present study,we investigated the changes of the coagulation state,bone microthrombus,microvascular bed and bone density levels in iron accumulation rats.Meanwhile,the effect of anticoagulation therapy on bone ...In the present study,we investigated the changes of the coagulation state,bone microthrombus,microvascular bed and bone density levels in iron accumulation rats.Meanwhile,the effect of anticoagulation therapy on bone mineral density was further investigated.We established two groups:a control(Ctrl)group and an iron intervention(FAC)group.Changes in coagulation function,peripheral blood cell counts,bone microthrombus,bone vessels and bone mineral density were compared between the two groups.We designed the non-treatment group and treatment group to study the changes of bone mineral density by preventing microthrombus formation with the anticoagulant fondaparinux.We found that the fbrinogen and D-dimer contents were significantly higher,whereas the thrombin time(TT)and prothrombin time(PT)were significantly shorter in the FAC group.After ink staining,the microvascular bed in the FAC group was significantly reduced compared with that in the Ctrl group.HE and Martius Scarlet Blue(MSB)staining showed microthrombus in the bone marrow of the iron accumulation rats.Following anticoagulation therapy,the bone microcirculation vascular bed areas in the treatment group rats were significantly increased.Furthermore,the bone mineral density was increased in the treatment group compared with that in the non-treatment group.Through experiments,we found that the blood in iron accumulation rat was relatively hypercoagulable;moreover,there was microthrombus in the bone marrow,and the bone vascular bed was reduced.Additionally,anticoagulation was helpful for improving bone microcirculation,reducing microthrombus and decreasing bone loss.展开更多
We found a mistake in Figure 6. Panels A (Sham group) and F (DFX group) (180degrees rotated) is same images. We have replaced the incorrect images (Panels F)with the correct Figure. This error does not change the mean...We found a mistake in Figure 6. Panels A (Sham group) and F (DFX group) (180degrees rotated) is same images. We have replaced the incorrect images (Panels F)with the correct Figure. This error does not change the meaning of the picture orthe conclusion of the manuscript. We apologize for our unintentional mistakes,which caused great inconvenience.展开更多
Postmenopausal osteoporosis is a kind of degenerative disease,also described as“invisible killer.”Estrogen is generally considered as the key hormone for women to maintain bone mineral content during their lives.Iro...Postmenopausal osteoporosis is a kind of degenerative disease,also described as“invisible killer.”Estrogen is generally considered as the key hormone for women to maintain bone mineral content during their lives.Iron accumulation refers to a state of human serum ferritin that is higher than the normal value but less than 1000μg/L.It has been found that iron accumulation and osteoporosis could occur simultaneously with the decrease in estrogen level after menopause.In recent years,many studies indicated that iron accumulation plays a vital role in postmenopausal osteoporosis,and a significant correlation has been found between iron accumulation and fragility fractures.In this review,we summarize and analyze the relevant literature including randomized controlled trials,systematic reviews,and meta-analyses between January 1996 and July 2022.We investigate the mechanism of the effect of iron accumulation on bone metabolism and discuss the relationship of iron accumulation,osteoporosis,and postmenopausal fragility fractures,as well as the main clinical treatment strategies.We conclude that it is necessary to pay attention to the phenomenon of iron accumulation in postmenopausal women with osteoporosis and explore the in-depth mechanism of abnormal bone metabolism caused by iron accumulation,in order to facilitate the discovery of effective therapeutic targets for postmenopausal osteoporosis.展开更多
The morphology is the consequence of evolution and adaptation.Escherichia coli is rod-shaped bacillus with regular dimension of about 1.5μm long and 0.5μm wide.Many shape-related genes have been identified and used ...The morphology is the consequence of evolution and adaptation.Escherichia coli is rod-shaped bacillus with regular dimension of about 1.5μm long and 0.5μm wide.Many shape-related genes have been identified and used in morphology engineering of this bacteria.However,little is known about if specific metabolism and metal irons could modulate bacteria morphology.Here in this study,we discovered filamentous shape change of E.coli cells overexpressing pigeon MagR,a putative magnetoreceptor and extremely conserved iron-sulfur protein.Comparative transcriptomic analysis strongly suggested that the iron metabolism change and iron accumulation due to the overproduction of MagR was the key to the morphological change.This model was further validated,and filamentous morphological change was also achieved by supplement E.coli cells with iron in culture medium or by increase the iron uptake genes such as entB and fepA.Our study extended our understanding of morphology regulation of bacteria,and may also serves as a prototype of morphology engineering by modulating the iron metabolism.展开更多
A 52-day continuous semi-static waterborne exposure（test media renewed daily） regimen was employed to investigate the accumulation and elimination profiles of two iron oxide nanomaterials（nano-Fe2O3 and nano-Fe3O4...A 52-day continuous semi-static waterborne exposure（test media renewed daily） regimen was employed to investigate the accumulation and elimination profiles of two iron oxide nanomaterials（nano-Fe2O3 and nano-Fe3O4） in zebrafish（Danio rerio）. Adult zebrafish were exposed to nanomaterial suspensions with initial concentrations of 4.0 and 10.0 mg/L for28 days and then were moved to clean water for 24 days to perform the elimination experiment. Fe content was measured in fish body and feces to provide data on accumulation and elimination of the two iron oxide nanomaterials in zebrafish. The experiment revealed that：（1） high accumulation of nano-Fe2O3 and nano-Fe3O4 were found in zebrafish, with maximum Fe contents, respectively, of 1.32 and 1.25 mg/g for 4.0 mg/L treatment groups and 1.15 and 0.90 mg/g for 10.0 mg/L treatment groups;（2） accumulated nanoparticles in zebrafish can be eliminated efficiently（the decrease of body burden of Fe conforms to a first-order decay equation） when fish were moved to nanoparticle-free water,and the elimination rates ranged from 86% to 100% by 24 days post-exposure; and（3）according to analysis of Fe content in fish excrement in the elimination phase, iron oxide nanomaterials may be adsorbed via the gastrointestinal tract, and stored for more than12 days.展开更多
基金supported by FIS PI16/00786(2016)and FIS PI19/00377(2019)grantsthe Ministerio de Sanidad,Spain and the Fondo Europeo de Desarrollo Regional(FEDER Unión Europea)Spanish Ministry of Education,Culture and Sport.This activity has been co-financed by the European Regional Development Fund(ERDF)and by the Regional Ministry of Economic Transformation,Industry,Knowledge and Universities of the Junta de Andalucía,within the framework of the ERDF Andalusia operational program 2014-2020 Thematic objective“01-Reinforcement of research,technological development and innovation”through the reference research project CTS-5725 and PY18-850(to JASA).
文摘Lipid peroxidation and iron accumulation are closely associated with neurodegenerative diseases,such as Alzheimer’s,Parkinson’s,and Huntington’s diseases,or neurodegeneration with brain iron accumulation disorders.Mitochondrial dysfunction,lipofuscin accumulation,autophagy disruption,and ferroptosis have been implicated as the critical pathomechanisms of lipid peroxidation and iron accumulation in these disorders.Currently,the connection between lipid peroxidation and iron accumulation and the initial cause or consequence in neurodegeneration processes is unclear.In this review,we have compiled the known mechanisms by which lipid peroxidation triggers iron accumulation and lipofuscin formation,and the effect of iron overload on lipid peroxidation and cellular function.The vicious cycle established between both pathological alterations may lead to the development of neurodegeneration.Therefore,the investigation of these mechanisms is essential for exploring therapeutic strategies to restrict neurodegeneration.In addition,we discuss the interplay between lipid peroxidation and iron accumulation in neurodegeneration,particularly in PLA2G6-associated neurodegeneration,a rare neurodegenerative disease with autosomal recessive inheritance,which belongs to the group of neurodegeneration with brain iron accumulation disorders.
文摘In the present study,we investigated the changes of the coagulation state,bone microthrombus,microvascular bed and bone density levels in iron accumulation rats.Meanwhile,the effect of anticoagulation therapy on bone mineral density was further investigated.We established two groups:a control(Ctrl)group and an iron intervention(FAC)group.Changes in coagulation function,peripheral blood cell counts,bone microthrombus,bone vessels and bone mineral density were compared between the two groups.We designed the non-treatment group and treatment group to study the changes of bone mineral density by preventing microthrombus formation with the anticoagulant fondaparinux.We found that the fbrinogen and D-dimer contents were significantly higher,whereas the thrombin time(TT)and prothrombin time(PT)were significantly shorter in the FAC group.After ink staining,the microvascular bed in the FAC group was significantly reduced compared with that in the Ctrl group.HE and Martius Scarlet Blue(MSB)staining showed microthrombus in the bone marrow of the iron accumulation rats.Following anticoagulation therapy,the bone microcirculation vascular bed areas in the treatment group rats were significantly increased.Furthermore,the bone mineral density was increased in the treatment group compared with that in the non-treatment group.Through experiments,we found that the blood in iron accumulation rat was relatively hypercoagulable;moreover,there was microthrombus in the bone marrow,and the bone vascular bed was reduced.Additionally,anticoagulation was helpful for improving bone microcirculation,reducing microthrombus and decreasing bone loss.
文摘We found a mistake in Figure 6. Panels A (Sham group) and F (DFX group) (180degrees rotated) is same images. We have replaced the incorrect images (Panels F)with the correct Figure. This error does not change the meaning of the picture orthe conclusion of the manuscript. We apologize for our unintentional mistakes,which caused great inconvenience.
基金Outstanding Youth Science Fund Project of the First Affiliated Hospital of Bengbu Medical College(No.2021byyfyyq04),China.
文摘Postmenopausal osteoporosis is a kind of degenerative disease,also described as“invisible killer.”Estrogen is generally considered as the key hormone for women to maintain bone mineral content during their lives.Iron accumulation refers to a state of human serum ferritin that is higher than the normal value but less than 1000μg/L.It has been found that iron accumulation and osteoporosis could occur simultaneously with the decrease in estrogen level after menopause.In recent years,many studies indicated that iron accumulation plays a vital role in postmenopausal osteoporosis,and a significant correlation has been found between iron accumulation and fragility fractures.In this review,we summarize and analyze the relevant literature including randomized controlled trials,systematic reviews,and meta-analyses between January 1996 and July 2022.We investigate the mechanism of the effect of iron accumulation on bone metabolism and discuss the relationship of iron accumulation,osteoporosis,and postmenopausal fragility fractures,as well as the main clinical treatment strategies.We conclude that it is necessary to pay attention to the phenomenon of iron accumulation in postmenopausal women with osteoporosis and explore the in-depth mechanism of abnormal bone metabolism caused by iron accumulation,in order to facilitate the discovery of effective therapeutic targets for postmenopausal osteoporosis.
基金supported by the National Natural Science Foundation of China(31640001 and T2350005 to C.X.,U21A20148 to X.Z.and C.X.)Ministry of Science and Technology of China(2021ZD0140300 to C.X.)Presidential Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(Y96XC11131,E26CCG27,and E26CCD15 to C.X.,E36CWGBR24B and E36CZG14132 to T.C.)。
文摘The morphology is the consequence of evolution and adaptation.Escherichia coli is rod-shaped bacillus with regular dimension of about 1.5μm long and 0.5μm wide.Many shape-related genes have been identified and used in morphology engineering of this bacteria.However,little is known about if specific metabolism and metal irons could modulate bacteria morphology.Here in this study,we discovered filamentous shape change of E.coli cells overexpressing pigeon MagR,a putative magnetoreceptor and extremely conserved iron-sulfur protein.Comparative transcriptomic analysis strongly suggested that the iron metabolism change and iron accumulation due to the overproduction of MagR was the key to the morphological change.This model was further validated,and filamentous morphological change was also achieved by supplement E.coli cells with iron in culture medium or by increase the iron uptake genes such as entB and fepA.Our study extended our understanding of morphology regulation of bacteria,and may also serves as a prototype of morphology engineering by modulating the iron metabolism.
基金supported by the Doctoral Education Funding of Education Ministry of China (No. 20070055033)
文摘A 52-day continuous semi-static waterborne exposure（test media renewed daily） regimen was employed to investigate the accumulation and elimination profiles of two iron oxide nanomaterials（nano-Fe2O3 and nano-Fe3O4） in zebrafish（Danio rerio）. Adult zebrafish were exposed to nanomaterial suspensions with initial concentrations of 4.0 and 10.0 mg/L for28 days and then were moved to clean water for 24 days to perform the elimination experiment. Fe content was measured in fish body and feces to provide data on accumulation and elimination of the two iron oxide nanomaterials in zebrafish. The experiment revealed that：（1） high accumulation of nano-Fe2O3 and nano-Fe3O4 were found in zebrafish, with maximum Fe contents, respectively, of 1.32 and 1.25 mg/g for 4.0 mg/L treatment groups and 1.15 and 0.90 mg/g for 10.0 mg/L treatment groups;（2） accumulated nanoparticles in zebrafish can be eliminated efficiently（the decrease of body burden of Fe conforms to a first-order decay equation） when fish were moved to nanoparticle-free water,and the elimination rates ranged from 86% to 100% by 24 days post-exposure; and（3）according to analysis of Fe content in fish excrement in the elimination phase, iron oxide nanomaterials may be adsorbed via the gastrointestinal tract, and stored for more than12 days.
