Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these val...Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these valuable chemicals, we have collected 110 varieties of mung bean seed samples and 8 mung bean products to determine the levels of these bioactive compounds. We also measured the DCI content in mung bean sprouts at different germination stages. Content of DCI, vitexin, and isovitexin in all mung bean varieties ranged from 0.43 to 5.79, 0.12 to 3.00, and 0.03 to 1.16 mg g-~, respectively. The varieties of C0001321, C0003522, and C0004485 have the highest DCI, vitexin, and isovitexin contents, respectively. The mung bean products in the market contained relatively lower level of these bioactive components. Contents of DCI, vitexin, and isovitexin in all mung bean products ranged from 0.119 to 0.717, 0 to 0.547, and 0 to 0.923 mg g-~, respectively. During the 112 h of germination test, DCI level steadily increased at first stage and reached the highest level at 80 h of germination （4.79 mg g-~）. These results provide useful information for the selection of suitable varieties and proper germination stages to obtain functional ingredients from mung beans.展开更多
Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, n...Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.展开更多
An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at...An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at a detection wavelength of 338 nm and a column temperature of 30℃.Rutin was chosen as the internal standard.The linear range of the standard curves was 0.20-128.75μg/mL in the plasma and 0.024-3.09μg/mL in the tissues.The LOQ was 0.19μg/mL in the plasma and 0.024μg/mL in the tissues.The relative recoveries of isovitexin ranged from 93% to 105% in the plasma and 87% to 112% in the tissues.The intra-and inter-day precisions were all below 8%.The pharmacokinetics and tissue distribution of isovitexin in rats were studied with the method.Blood samples were collected at fixed time intervals after the i.v.injection of isovitexin at a dosage of 18.75,3.75 and 0.75 mg/kg;the tissue samples（brain,liver,kidney,heart,lung,spleen and ovary）were obtained at 10,30,and 60 min after the i.v.injection of isovitexin at a dosage of 18.75 mg/kg.The pharmacokinetics of the isovitexin in three different dosages in the rats fit the two-compartment open model.The isovitexin displayed linear dynamics in the dosage range of 0.75-18.75 mg/kg.The mean value of t_（1/2α）was 1.54-1.84 min,and t_（1/2β）was 36.94-46.27 min at the three dosages.The tissue distribution study showed that the sequence of tissue drug concentration from high to low was kidneyliverlung≈ovaryheart≈spleenbrain.展开更多
Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since...Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.展开更多
Background The proliferation of porcine ovarian granulosa cells(GCs)is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle homeostasis.Previous studies foun...Background The proliferation of porcine ovarian granulosa cells(GCs)is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle homeostasis.Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1(UCHL1)regulates female reproduction,especially in ovarian development.However,the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.Results UCHL1 overexpression promoted GC proliferation,and knockdown had the opposite effect.UCHL1 is directly bound to cyclin B1(CCNB1),prolonging the half-life of CCNB1 and inhibiting its degradation,thereby promoting GC proliferation.What's more,a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.Conclusions UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1,and isovitexin enhanced the enzyme activity of UCHL1.These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.展开更多
建立了测定木豆叶、茎、根中牡荆苷和异牡荆苷含量的高效液相色谱分析方法。色谱柱为HIQ Sil C18V(250mm×4.6mm,5μm);流动相为甲醇-水-甲酸(体积比35∶64.74∶0.26);检测波长330nm;流速1mL/min;进样量10μL;柱温30℃。在2～200mg/...建立了测定木豆叶、茎、根中牡荆苷和异牡荆苷含量的高效液相色谱分析方法。色谱柱为HIQ Sil C18V(250mm×4.6mm,5μm);流动相为甲醇-水-甲酸(体积比35∶64.74∶0.26);检测波长330nm;流速1mL/min;进样量10μL;柱温30℃。在2～200mg/L牡荆苷、异牡荆苷质量浓度与色谱峰面积线性关系良好;牡荆苷峰面积精密度的相对标准偏差(RSD)最大为2.93%,重复性RSD为2.91%,加样回收率为97.38%;异牡荆苷峰面积精密度的RSD最大为2.69%,重复性RSD为3.37%,加样回收率为98.63%。木豆叶、茎、根中牡荆苷、异牡荆苷的质量分数分别为0.768、0.066、0.183mg/g和0.799、0.139、0.013mg/g。叶中牡荆苷和异牡荆苷含量明显高于茎、根中的含量,可以作为大规模获得牡荆苷、异牡荆苷的资源。展开更多
基金supported by the Talent Fund(to Dr. Ren Guixing) from the Chinese Academy of Agricultural Sciencesthe Earmarked Fund for Modern Agro-Industry Technology Research System,China(nycytx-018)
文摘Mung bean （Vigna radiata L.） is rich in bioactive compounds including D-chiro-inositol （DCI）, vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these valuable chemicals, we have collected 110 varieties of mung bean seed samples and 8 mung bean products to determine the levels of these bioactive compounds. We also measured the DCI content in mung bean sprouts at different germination stages. Content of DCI, vitexin, and isovitexin in all mung bean varieties ranged from 0.43 to 5.79, 0.12 to 3.00, and 0.03 to 1.16 mg g-~, respectively. The varieties of C0001321, C0003522, and C0004485 have the highest DCI, vitexin, and isovitexin contents, respectively. The mung bean products in the market contained relatively lower level of these bioactive components. Contents of DCI, vitexin, and isovitexin in all mung bean products ranged from 0.119 to 0.717, 0 to 0.547, and 0 to 0.923 mg g-~, respectively. During the 112 h of germination test, DCI level steadily increased at first stage and reached the highest level at 80 h of germination （4.79 mg g-~）. These results provide useful information for the selection of suitable varieties and proper germination stages to obtain functional ingredients from mung beans.
基金supported by the Talent Fund(to Dr. Ren Guixing)from the Chinese Academy of Agricultural Sciencesthe Earmarked Fund for Modern Agro-Industry Technology Research System,China(nycytx-018)
文摘Mung bean （Vigna radiata L.） is rich in D-chiro-inositol （DCI）, vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy （NIRS） was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography （HPLC）. The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares （PLS） algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions （R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin）. The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.
基金Foundation for Distinguished Young Talents in Higher Education of Guangdong and Foundation for Young Scholars of Guangdong Medical College(Grant No XQ0802)
文摘An HPLC method for the determination of isovitexin in rat plasma and different tissues was developed.The separation was achieved on a C_（18）column with a mobile phase consisting of methanol-1% acetum（40：60,v/v）at a detection wavelength of 338 nm and a column temperature of 30℃.Rutin was chosen as the internal standard.The linear range of the standard curves was 0.20-128.75μg/mL in the plasma and 0.024-3.09μg/mL in the tissues.The LOQ was 0.19μg/mL in the plasma and 0.024μg/mL in the tissues.The relative recoveries of isovitexin ranged from 93% to 105% in the plasma and 87% to 112% in the tissues.The intra-and inter-day precisions were all below 8%.The pharmacokinetics and tissue distribution of isovitexin in rats were studied with the method.Blood samples were collected at fixed time intervals after the i.v.injection of isovitexin at a dosage of 18.75,3.75 and 0.75 mg/kg;the tissue samples（brain,liver,kidney,heart,lung,spleen and ovary）were obtained at 10,30,and 60 min after the i.v.injection of isovitexin at a dosage of 18.75 mg/kg.The pharmacokinetics of the isovitexin in three different dosages in the rats fit the two-compartment open model.The isovitexin displayed linear dynamics in the dosage range of 0.75-18.75 mg/kg.The mean value of t_（1/2α）was 1.54-1.84 min,and t_（1/2β）was 36.94-46.27 min at the three dosages.The tissue distribution study showed that the sequence of tissue drug concentration from high to low was kidneyliverlung≈ovaryheart≈spleenbrain.
基金This study was supported by the Key Project of Natural Science Research for Colleges and Universities in Anhui Province(KJ2021A0533,2023AH050345)the Excellent Scientific Research and Innovation Team of Universities in Anhui Province(2022AH010029).
文摘Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.
基金funded by National Key R&D Program of China(NO.2022YFD1300303)National Natural Science Foundation of China(32272849)。
文摘Background The proliferation of porcine ovarian granulosa cells(GCs)is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle homeostasis.Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1(UCHL1)regulates female reproduction,especially in ovarian development.However,the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.Results UCHL1 overexpression promoted GC proliferation,and knockdown had the opposite effect.UCHL1 is directly bound to cyclin B1(CCNB1),prolonging the half-life of CCNB1 and inhibiting its degradation,thereby promoting GC proliferation.What's more,a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.Conclusions UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1,and isovitexin enhanced the enzyme activity of UCHL1.These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.
文摘建立了测定木豆叶、茎、根中牡荆苷和异牡荆苷含量的高效液相色谱分析方法。色谱柱为HIQ Sil C18V(250mm×4.6mm,5μm);流动相为甲醇-水-甲酸(体积比35∶64.74∶0.26);检测波长330nm;流速1mL/min;进样量10μL;柱温30℃。在2～200mg/L牡荆苷、异牡荆苷质量浓度与色谱峰面积线性关系良好;牡荆苷峰面积精密度的相对标准偏差(RSD)最大为2.93%,重复性RSD为2.91%,加样回收率为97.38%;异牡荆苷峰面积精密度的RSD最大为2.69%,重复性RSD为3.37%,加样回收率为98.63%。木豆叶、茎、根中牡荆苷、异牡荆苷的质量分数分别为0.768、0.066、0.183mg/g和0.799、0.139、0.013mg/g。叶中牡荆苷和异牡荆苷含量明显高于茎、根中的含量,可以作为大规模获得牡荆苷、异牡荆苷的资源。
