The constant evolution of pathogens poses a threat to wheat resistance against diseases,endangering food security.Developing resistant wheat varieties is the most practical approach for circumventing this problem.As a...The constant evolution of pathogens poses a threat to wheat resistance against diseases,endangering food security.Developing resistant wheat varieties is the most practical approach for circumventing this problem.As a close relative of wheat,Aegilops geniculata,particularly accession SY159,has evolved numerous beneficial traits that could be applied to improve wheat.In this study,we established the karyotype of SY159 by fluorescence in situ hybridization(FISH)using the oligonucleotide probes Oligo-pTa535 and Oligo-pSc119.2 and a complete set of wheat–Ae.geniculata accession TA2899 addition lines as a reference.Using specific-locus amplified fragment sequencing(SLAF-seq)technology,400 specific markers were established for detecting the SY159 chromosomes with efficiencies reaching 81.5%.The SY159-specific markers were used to classify the different homologous groups of SY159 against the wheat-Ae.geniculata addition lines.We used these specific markers on the 7Mg chromosome after classification,and successfully confirmed their suitability for studying the different chromosomes of SY159.This study provides a foundation for accelerating the application of SY159 in genetic breeding programs designed to improve wheat.展开更多
Karyotype prescription is based on clinical signs (or reasons for karyotype prescription) which are phenotypic manifestations associated with chromosomal abnormalities. The aim of this study was to establish a corresp...Karyotype prescription is based on clinical signs (or reasons for karyotype prescription) which are phenotypic manifestations associated with chromosomal abnormalities. The aim of this study was to establish a correspondence between karyotype indications and their results in patients. This was a retrospective study that was carried out in the Histology-Embryology-Cytogenetics laboratory of the University Hospital of Cocody-Abidjan from 2014 to 2019. 58 patient files were identified and included the indication or reason for prescribing a constitutional karyotype and the biological result obtained. An individual data sheet was used to collect the data. 17 reasons for prescription were identified and divided into 2 groups. Sexual ambiguity was the most frequent reason (29.3%). The first group (G1) represented the 10 reasons for which the karyotype results were normal. The second group (G2) corresponded of the 7 motives with normal or abnormal karyotype results. Several anomalies were listed according to these reasons: inversions, mosaics (anomalies of number and structure) and trisomy 21. The last was the most frequent chromosomal anomaly (69.24%). It was found in several reasons for karyotype prescription: malformations, neurological disorders, suspected trisomy and cardiac pathology. Several factors could explain these results, among which are the limits of the karyotype and the non-genetic causes that can induce these abnormal phenotypes. Complementary examinations to the karyotype are molecular cytogenetic techniques, notably fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (Array-CGH).展开更多
[Objective] The research aimed to study the morphological characteristics and karyotype of Pelteobagrus fulvidraco in Dongting Lake water system.[Method] By using the conventional biological morphometry,PHA and colchi...[Objective] The research aimed to study the morphological characteristics and karyotype of Pelteobagrus fulvidraco in Dongting Lake water system.[Method] By using the conventional biological morphometry,PHA and colchicine injection method in vivo,the morphological characteristics and karyotype of P.fulvidraco in Yuanshui River and Lishui River of Dongting Lake were analyzed.[Result] In three ratio traits including standard length/head length,standard length/caudal peduncle depth,head length/snout length,P.fulvidraco of Yuanshui River and Lishui River had significant differences（P0.05）.However,the number and karyotype of their chromosomes were same.The chromosome number was 2n = 52,and the karyotype formula was 20M＋12SM＋10ST＋10T.The number of chromosome arm was 84.[Conclusion] The research result had certain theoretical guidance significance for the protection and utilization of wild P.resource in Dongting Lake water system.展开更多
The karyotype of two Chinese species in Orius were studied in male germ cells prepared on air dried slides stained with Giemsa. It is reported that two species have 24 chromosomes in diploid and X Y sex chromoso...The karyotype of two Chinese species in Orius were studied in male germ cells prepared on air dried slides stained with Giemsa. It is reported that two species have 24 chromosomes in diploid and X Y sex chromosomal mechanism. But the beha vior of chromosomes during meiosis between two species is different. Which show in the arrangment and location of the X chromosome and autosome at the late diakinesis as well as metaphase stage respectively and so on. These characteristics may be used in ta xonomy among species of Orius .展开更多
Karyotype of 3 species of the genus Orius Wolff were firstly studied using the Giemsa staining and squashing slides method in male germ cell. It is reported that number of chromosome and system of sex chromosome of ha...Karyotype of 3 species of the genus Orius Wolff were firstly studied using the Giemsa staining and squashing slides method in male germ cell. It is reported that number of chromosome and system of sex chromosome of haploid chromosome complement of these species is n=11A＋X （Y） in the first meiotic stage. However, the behavior of chromosomes among the species are different distinctly, thus cytotaxonomic character can be adopted to the identification among the three species.展开更多
Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one wa...Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one was 1.31; the proportions of chromosomes with arm ratio higher than 2 was 0.08; the asymmetry index was 57.02; the karyotype type was 2A; and the karyotype formula was 2n=-24=20m＋4sm.展开更多
[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head...[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.展开更多
Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationsh...Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationships among 8 subgroups of D. melanogaster species group were analyzed systematically. The results were as follows. The basic karyotype of elegans subgroup was type A. The karyotypes of eugracilis subgroup, melanogaster subgroup, and ficusphila subgroup were all type C. The karyotypes of takahashii subgroup and suzukii subgroup were both type C and type D. The montium subgroup had six kinds of karyotypes types B, C, C＇, D, D＇, and E. The ananassae subgroup had three kinds of karyotypes： types F, G, and H. Thus, the melanogaster species group was classified into five pedigrees based on the diversity of these karyotypes： 1） elegans; 2） eugracilis-melanogasterficusphila; 3） takkahashii-suzukii; 4） montium; 5） ananassae. The above-mentioned results in karyotypic evolution were consistent with those of DNA sequence analysis reported by Yang except for the elegans subgroup and this subgroup was considered as the ancestral subgroup. Karyotype analysis of the same drosophila from different isofemale lines indicated that the same Drosophila from different places showed karyotypic variation which might be due to different geographical environment and evolutionary degree or interaction between the two factors.展开更多
[Objective]This study was to analyze the karyotypes of wild diploid strawberry species,so as to provide basis for revealing the ori- gins,evolvement and genetic breeding of strawberry. [Method] Four diploid strawberry...[Objective]This study was to analyze the karyotypes of wild diploid strawberry species,so as to provide basis for revealing the ori- gins,evolvement and genetic breeding of strawberry. [Method] Four diploid strawberry species including Fragaria gracilis A. Los,Fragaria nilgerrensis Schidl,Fragaria pentaphylla A. Los. and Fragaria vesca L. were used as experimental materials to analyze their chromosomal kary- otypes. [Result]The karyotype formula for strawberry species tested was as follows： 2n =2x =14 =10m ＋2sm ＋2m＊ for F. gracilis A. Los, belonging to type 1B; 2n =2x =14 =14m for F. nilgerrensis Schidl,belonging to type 1A; 2n =2x =14 =12m ＋2sm for F. pentaphylla A. Loz. , belonging to type 1A; 2n =2x =14 =14m for F. vesca L. ,belonging to type 1A. Karyotype analysis showed that karyotypic asymmetry degree of the four species was F. gracilis A. Los. F. pentaphylla A. Loz. F. vesca L. F. nilgerrensis Schidl. [Conclusion] The evolution order of these four species was probably F. gracilis A. Los. ,F. pentaphylla A. Loz. ,F. vesca L. ,F. nilgerrensis Schidl.展开更多
[Objective] The aim of this study was to investigate the chromosome number and the karyotype of Scilla scilloides in Yantai.[Method]Root tips of Scilla scilloides were pretreated by 8-hydroxyquinoline,then fixed,disso...[Objective] The aim of this study was to investigate the chromosome number and the karyotype of Scilla scilloides in Yantai.[Method]Root tips of Scilla scilloides were pretreated by 8-hydroxyquinoline,then fixed,dissociated and stained for slice production.The chromosome number was analyzed by microscopic examination,and then cells with good chromosomal morphology and dispersal chromosome were studied by microscopic photos.[Result]The somatic chromosome number of Scilla scilloides in Yantai was 2n=16+1Bs,while the karyotype formula was K(2n)=2x=16+1Bs=6m+4sm(2SAT)+4st+2t+1Bs and the karyotype classification was "3B" type.[Conclusion]Karyotype comparison,division of cell type and evolution of Scilla scilloides in Yantai are discussed,which provides basis for cytogenetics,evolutionary genetics,modern taxonomy and genetic breeding.展开更多
The chromosome karyotypes of Chorthippus （Megaulacobothrus） aethalinus （Zubovsky） and Ch. （M.） chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n （♂） = 17 = ...The chromosome karyotypes of Chorthippus （Megaulacobothrus） aethalinus （Zubovsky） and Ch. （M.） chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n （♂） = 17 = 16 ＋ XO, in which three pairs of autosomal and sex chromosomes were terminal chromosomes, and the other five pairs of autosomes were metacentric chromosomes. These are diagnosis characters for Chorthippus. However, these two species can be distinguished by the different chromosome formula （CF） and the relative length （RL）. The CF in Ch. （M.） aethalinus is K （2n, ♂） = 6m ＋ 11t = 6L ＋ 6M ＋ 4S ＋ XO, whereas that of Ch. （M.） chinensis is K （2n, ♂） = 6m ＋ 11t = 6L ＋ 8M ＋ 2S ＋ XO. In addition, we also found that the relative lengths of sex chromosomes in the two species were different from each other. The sex-chromosome of Ch. （M.） aethalinus is located at the fifth position and its relative length is 8.33% whereas that of Ch. （M.） chinensis is at the eighth position and its relative length is 5.53%. These results show that significantly different genetic features exist for the two species of Chorthippus.展开更多
[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan...[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.展开更多
A new species, Hedotettix nujiangensis Zheng sp. nov., is described. The chromosome complement of H. nujiangensis consists of 2n （♂） = 13. Sex determination is XO. All chromosomes are telocentric （T） and the sex ...A new species, Hedotettix nujiangensis Zheng sp. nov., is described. The chromosome complement of H. nujiangensis consists of 2n （♂） = 13. Sex determination is XO. All chromosomes are telocentric （T） and the sex chromosome is the fourth element in size. Type specimens are deposited at Southwest Forestry University.展开更多
The chromosome karyotypes of Chorthippus albomarginatus （De Geer） which represents the genus Chorthippus Fieber and Euchorthippus unicolor （Ikonnikov） which represents the genus Euchorthippus Tarbinsky were compar...The chromosome karyotypes of Chorthippus albomarginatus （De Geer） which represents the genus Chorthippus Fieber and Euchorthippus unicolor （Ikonnikov） which represents the genus Euchorthippus Tarbinsky were compared. The chromosome numbers for these two species fit 2n （♂） = 17 = 16 ＋ XO, in which five pairs of autosomal and sex chromosomes are terminal chromosomes, and the other three pairs of autosomes are metacentric chromosomes. This similarity between the two genera indicates that they have a close phylogenetic relationship. However, these two species can be distinguished by their different chromosome formula （CF） and the relative sex chromosome length （RL）. The CF in C. albomarginatus is K （2n,♂） = 6m ＋ 11t = 8L ＋ 4M ＋ 4S ＋ XO, whereas that ofE. unicolor is K （2n, ♂） = 6m ＋ 11t = 8L ＋ 6M ＋ 2S ＋ XO. The relative lengths of sex chromosomes in the two species are different. The sex-chromosome of C. albomarginatus is located at the ninth position and its relative length is 2.96% whereas that ofE. unicolor is at the eighth position and its relative length is 4.26%. These results show that significantly different genetic features exist for the two genera.展开更多
The chromosomes of three odor frogs, Luetuosae- group of Rana were analyzed by conventional, as well as Obanding and silver staining techniques. The three species here examined all had 26 chromosome karyotypes encompa...The chromosomes of three odor frogs, Luetuosae- group of Rana were analyzed by conventional, as well as Obanding and silver staining techniques. The three species here examined all had 26 chromosome karyotypes encompassing 5 large and 8 small homologous pairs. Differences among them were found in gross shapes of chromosomes and positions of secondary constrictions. Generally speaking, karyotypes of R. andersonii and R. grahami resembled each other, while the karyotype of R. tiannanensis differed from the former two in several respects.Analyses of karyotypes by C-banding technique indicated that, centromeric areas of every chromosome and interstitial parts of some chromosomes of each species were hetero-chromatinized, and differences of distribution of heterochromatin were found among species. In early metaphase plates of R. andersonii, much more heterochromatinized areas were observed, and when it reached late metaphase, the numbers of heterochromatin sections in each chromosome pair reduced to a limited level, e. g. centromeres and a few interstitial parts.The active nucleolar organizer regions (NORs) were localized in long arms of pair No. 10, in connection with secondary constrictions for R. andersonii and R. grahami as in other odor frogs previously reported, but in the long arm of pair No. 6 for R. tiannanensis.The cytogenetic and taxonomic implications of the findings were discussed based on comparisons with each other, and with published literature.展开更多
We examined mitotic chromosomes of three newly found members of the Drosophila obscura species group,D.luguensis,D.dianensis and D.limingi from Yunnan and compared their karyotypes to those of respective related speci...We examined mitotic chromosomes of three newly found members of the Drosophila obscura species group,D.luguensis,D.dianensis and D.limingi from Yunnan and compared their karyotypes to those of respective related species.D.luguensis possessed a diploid number of 12 chromosomes,comprising 3 pairs of metacentrics (V-shaped),2 pairs of acrocentrics (rod-like) and 1 pair of micro-chromosomes (dot-like).Both the X and Y were metacentrics.D.dianensis and D.limingi possessed a diploid number of 10 chromosomes,comprising 1 pair of large V-shaped,1 pair of moderate-sized V-shaped,2 pairs of submetacentric (J-shaped) chromosomes,and 1 pairs of dot-like chromosomes,with their X chromosomes were J-shaped and Y chromosomes were short rod-like ones.Based upon the results of chromosomal comparisons,geographic information of the sinobscura subgroup,as well inter-specific phylogenetic relationship deduced elsewhere,D.luguensis was considered to retain an ancestral form of the karyotype within this subgroup.The present observations have also implied that D.sinobscura (2n=12:2V,1J,2R,1D) might have derived from a sub-lineage of a pre-“sinobscura-hubeiensis” lineage by pericentric inversion of moderate-sized metacentric autosomes and D.hubeiensis (2n=10:4V,1D) from another sub-lineage of the same lineage by centric fusion of 2 pairs of acrocentric autosomes.Similar chromosomal changes of centric fusion,centric fission and/or pericentric inversion might have occurred during the course of species divergences between D.dianensis and D.subsilvestris (2n=12:3V,2R,1D),an European member closely related to D.dianensis,and between D.limingi and D.tsukubaensis (2n=12:3V,2R,1D),an East Asian member near to D.limingi.展开更多
[Objective] This study aimed to compare the similarities and dissimilarities in karyotypes of purple and white onions,so as to lay foundation for further studying its genetic improvement and breeding more onion variet...[Objective] This study aimed to compare the similarities and dissimilarities in karyotypes of purple and white onions,so as to lay foundation for further studying its genetic improvement and breeding more onion varieties.[Method] Sections of root-tip cells were prepared by normal wafer method.Photomicrography was adopted to produce the high-quality chromosome specimen.Finally,karyotype analysis was conducted using Adobe Photoshop.[Result] Chromosome karyotype analysis on the root-tip showed that the chromosome number of both purple and white onions was 2n=2x=16.Among the 16 chromosomes of both the onions,no satellite chromosome was found.By measuring and calculating,it was found that the karyotype of the studied white union belonged to the lowly evolutional 2A type,and its karyotype formula was 2n=2x=16=14 m ＋2St,with the karyotype asymmetry coefficient of 58.67%.No obvious centromere was observed in the sections of purple onion.[Conclusion] The karyotypes of white and purple unions are similar and we can infer from this fact that the reason of different characters between both the onions is not due to the chromosome aberration.This karyotype formula and asymmetrical karyotype coefficient have some reference values on the identification of onion variety resources.展开更多
Elytrigia Desv. is widely distributed throughout the world and is represented with species of various levels of ploidy including diploids, tetraploids, hexaploids, octaploids, and decaploids. The distribution pattern ...Elytrigia Desv. is widely distributed throughout the world and is represented with species of various levels of ploidy including diploids, tetraploids, hexaploids, octaploids, and decaploids. The distribution pattern of these ploidy levels, however, is not well-defined. In this study, the levels of ploidy for 64 accessions of Elytrigia from 25 countries were determined with microscopic procedures. The results showed that accessions of E. intermedia and E. repens were grouped into three distinct levels of ploidy including diploids, tetraploids and hexaploids. For E. elongata, E. pontica, and E. caespitosa, it was found that two ploidy levels presented, and only one ploidy level was in those of E. hybrid, E. pycnantha, E. pungens, E. juncea, and E. alatavica. Karyotype analysis indicated that the karyotype formula of diploid, tetraploid and hexaploid of E. intermedia was 2n = 2x = 14 = 6m ＋ 6sm ＋ 2st, 2n = 4x = 28 = 2M ＋ 10m ＋ 16sm and 2n = 6x = 42 = 4M ＋ 18m ＋ 20sin, respectively. Furthermore, the karyotype formula of three germplasms in tetraploid of E. intermedia was 2n=4x=28 =2M＋ 10m＋ 16sm, 2n=4x=28=4M＋22m （sat）＋2sm and 2n=4x=28 =4M＋ 12m＋ 12sm （sat）, which were not completely uniform. Therefore, it could be suggested that the studies about chromosome constitution would be helpful for the detail understanding of the diversity of germplasm resource in Elytrigia and promoting the utilization in the crop molecular breeding.展开更多
The karyotypes of 27 individuals of Elymus nutans from eight wild populations in the Qinghai Plateau were analyzed using sequential FISH and GISH. High FISH pattern polymorphism and karyotype variation were detected w...The karyotypes of 27 individuals of Elymus nutans from eight wild populations in the Qinghai Plateau were analyzed using sequential FISH and GISH. High FISH pattern polymorphism and karyotype variation were detected within and among populations. The chromosome variations were mainly characterized as repeat deletions and amplifications along with inter-genomic translocations. The chromosomes of the St and Y genomes demonstrated higher polymorphism than those of the H genome. Six different intergenomic translocations were identified in 33.3% of individuals; type Ⅰ and Ⅱ translocations were detected with higher frequency. Further analysis revealed that type Ⅰ and Ⅱ translocations were distributed in different geographic regions. The origin of high karyotype variation of E. nutans in the Qinghai plateau is further discussed.展开更多
The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this p...The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants.展开更多
基金funded by the National Natural Science Foundation of China(31471481)the Project of Science and Technology of Shaanxi Province of China(2021NY-081 and 2023YBNY-033)。
文摘The constant evolution of pathogens poses a threat to wheat resistance against diseases,endangering food security.Developing resistant wheat varieties is the most practical approach for circumventing this problem.As a close relative of wheat,Aegilops geniculata,particularly accession SY159,has evolved numerous beneficial traits that could be applied to improve wheat.In this study,we established the karyotype of SY159 by fluorescence in situ hybridization(FISH)using the oligonucleotide probes Oligo-pTa535 and Oligo-pSc119.2 and a complete set of wheat–Ae.geniculata accession TA2899 addition lines as a reference.Using specific-locus amplified fragment sequencing(SLAF-seq)technology,400 specific markers were established for detecting the SY159 chromosomes with efficiencies reaching 81.5%.The SY159-specific markers were used to classify the different homologous groups of SY159 against the wheat-Ae.geniculata addition lines.We used these specific markers on the 7Mg chromosome after classification,and successfully confirmed their suitability for studying the different chromosomes of SY159.This study provides a foundation for accelerating the application of SY159 in genetic breeding programs designed to improve wheat.
文摘Karyotype prescription is based on clinical signs (or reasons for karyotype prescription) which are phenotypic manifestations associated with chromosomal abnormalities. The aim of this study was to establish a correspondence between karyotype indications and their results in patients. This was a retrospective study that was carried out in the Histology-Embryology-Cytogenetics laboratory of the University Hospital of Cocody-Abidjan from 2014 to 2019. 58 patient files were identified and included the indication or reason for prescribing a constitutional karyotype and the biological result obtained. An individual data sheet was used to collect the data. 17 reasons for prescription were identified and divided into 2 groups. Sexual ambiguity was the most frequent reason (29.3%). The first group (G1) represented the 10 reasons for which the karyotype results were normal. The second group (G2) corresponded of the 7 motives with normal or abnormal karyotype results. Several anomalies were listed according to these reasons: inversions, mosaics (anomalies of number and structure) and trisomy 21. The last was the most frequent chromosomal anomaly (69.24%). It was found in several reasons for karyotype prescription: malformations, neurological disorders, suspected trisomy and cardiac pathology. Several factors could explain these results, among which are the limits of the karyotype and the non-genetic causes that can induce these abnormal phenotypes. Complementary examinations to the karyotype are molecular cytogenetic techniques, notably fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (Array-CGH).
基金Supported by National Natural Science Foundation of China(30972260)Science and Technology Innovation Team Plan of Hunan Higher Education(2010)~~
文摘[Objective] The research aimed to study the morphological characteristics and karyotype of Pelteobagrus fulvidraco in Dongting Lake water system.[Method] By using the conventional biological morphometry,PHA and colchicine injection method in vivo,the morphological characteristics and karyotype of P.fulvidraco in Yuanshui River and Lishui River of Dongting Lake were analyzed.[Result] In three ratio traits including standard length/head length,standard length/caudal peduncle depth,head length/snout length,P.fulvidraco of Yuanshui River and Lishui River had significant differences（P0.05）.However,the number and karyotype of their chromosomes were same.The chromosome number was 2n = 52,and the karyotype formula was 20M＋12SM＋10ST＋10T.The number of chromosome arm was 84.[Conclusion] The research result had certain theoretical guidance significance for the protection and utilization of wild P.resource in Dongting Lake water system.
基金The work was supported by National Natural Science Foundation of China( 395 70 1 0 8) for second author and Zhejiang Forestry College Science Foundation
文摘The karyotype of two Chinese species in Orius were studied in male germ cells prepared on air dried slides stained with Giemsa. It is reported that two species have 24 chromosomes in diploid and X Y sex chromosomal mechanism. But the beha vior of chromosomes during meiosis between two species is different. Which show in the arrangment and location of the X chromosome and autosome at the late diakinesis as well as metaphase stage respectively and so on. These characteristics may be used in ta xonomy among species of Orius .
基金The work was supported by Zhejiang Forestry College Science Foundation
文摘Karyotype of 3 species of the genus Orius Wolff were firstly studied using the Giemsa staining and squashing slides method in male germ cell. It is reported that number of chromosome and system of sex chromosome of haploid chromosome complement of these species is n=11A＋X （Y） in the first meiotic stage. However, the behavior of chromosomes among the species are different distinctly, thus cytotaxonomic character can be adopted to the identification among the three species.
基金Supported by National Natural Science Foundation of China(31100401)Tianjin Science and Technology Plan Project(13ZLZLZF05700)+1 种基金Fund for Special Science and Technology Correspondents of Tianjin City(15JCTPJC59500)College Students'Innovative Entrepreneurial Training Program of Tianjin City(201610061102)~~
文摘Karyotype analysis of Lycium ruthenicum Murr. was carried out in this study. The results showed that the chromosome number was 2n=2x=24; the arm index was 48; the ratio of the longest chromosome to the shortest one was 1.31; the proportions of chromosomes with arm ratio higher than 2 was 0.08; the asymmetry index was 57.02; the karyotype type was 2A; and the karyotype formula was 2n=-24=20m＋4sm.
基金Supported by National Natural Science Foundation of China (31172074,30700071)Natural Science Foundation of Shandong Province (ZR2010CL002)~~
文摘[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.
基金This work was supported by National Natural Sciences Foundation of China (No. 39930100) International Collaborative Project of Hubei Provincial Department of Education (No. G200610001).
文摘Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationships among 8 subgroups of D. melanogaster species group were analyzed systematically. The results were as follows. The basic karyotype of elegans subgroup was type A. The karyotypes of eugracilis subgroup, melanogaster subgroup, and ficusphila subgroup were all type C. The karyotypes of takahashii subgroup and suzukii subgroup were both type C and type D. The montium subgroup had six kinds of karyotypes types B, C, C＇, D, D＇, and E. The ananassae subgroup had three kinds of karyotypes： types F, G, and H. Thus, the melanogaster species group was classified into five pedigrees based on the diversity of these karyotypes： 1） elegans; 2） eugracilis-melanogasterficusphila; 3） takkahashii-suzukii; 4） montium; 5） ananassae. The above-mentioned results in karyotypic evolution were consistent with those of DNA sequence analysis reported by Yang except for the elegans subgroup and this subgroup was considered as the ancestral subgroup. Karyotype analysis of the same drosophila from different isofemale lines indicated that the same Drosophila from different places showed karyotypic variation which might be due to different geographical environment and evolutionary degree or interaction between the two factors.
基金Supported by Program for Excellent Scholars Returned from Overseas~~
文摘[Objective]This study was to analyze the karyotypes of wild diploid strawberry species,so as to provide basis for revealing the ori- gins,evolvement and genetic breeding of strawberry. [Method] Four diploid strawberry species including Fragaria gracilis A. Los,Fragaria nilgerrensis Schidl,Fragaria pentaphylla A. Los. and Fragaria vesca L. were used as experimental materials to analyze their chromosomal kary- otypes. [Result]The karyotype formula for strawberry species tested was as follows： 2n =2x =14 =10m ＋2sm ＋2m＊ for F. gracilis A. Los, belonging to type 1B; 2n =2x =14 =14m for F. nilgerrensis Schidl,belonging to type 1A; 2n =2x =14 =12m ＋2sm for F. pentaphylla A. Loz. , belonging to type 1A; 2n =2x =14 =14m for F. vesca L. ,belonging to type 1A. Karyotype analysis showed that karyotypic asymmetry degree of the four species was F. gracilis A. Los. F. pentaphylla A. Loz. F. vesca L. F. nilgerrensis Schidl. [Conclusion] The evolution order of these four species was probably F. gracilis A. Los. ,F. pentaphylla A. Loz. ,F. vesca L. ,F. nilgerrensis Schidl.
基金Supported by the Research Fund of Ludong University(043312)~~
文摘[Objective] The aim of this study was to investigate the chromosome number and the karyotype of Scilla scilloides in Yantai.[Method]Root tips of Scilla scilloides were pretreated by 8-hydroxyquinoline,then fixed,dissociated and stained for slice production.The chromosome number was analyzed by microscopic examination,and then cells with good chromosomal morphology and dispersal chromosome were studied by microscopic photos.[Result]The somatic chromosome number of Scilla scilloides in Yantai was 2n=16+1Bs,while the karyotype formula was K(2n)=2x=16+1Bs=6m+4sm(2SAT)+4st+2t+1Bs and the karyotype classification was "3B" type.[Conclusion]Karyotype comparison,division of cell type and evolution of Scilla scilloides in Yantai are discussed,which provides basis for cytogenetics,evolutionary genetics,modern taxonomy and genetic breeding.
基金supported by the Special fund for basic scientific research project in the central scientific research institutes (Institute of Grassland Research of CAAS)(1610332012007)
文摘The chromosome karyotypes of Chorthippus （Megaulacobothrus） aethalinus （Zubovsky） and Ch. （M.） chinensis Tarbinsky were compared. Results show that the chromosome numbers of the two species fit 2n （♂） = 17 = 16 ＋ XO, in which three pairs of autosomal and sex chromosomes were terminal chromosomes, and the other five pairs of autosomes were metacentric chromosomes. These are diagnosis characters for Chorthippus. However, these two species can be distinguished by the different chromosome formula （CF） and the relative length （RL）. The CF in Ch. （M.） aethalinus is K （2n, ♂） = 6m ＋ 11t = 6L ＋ 6M ＋ 4S ＋ XO, whereas that of Ch. （M.） chinensis is K （2n, ♂） = 6m ＋ 11t = 6L ＋ 8M ＋ 2S ＋ XO. In addition, we also found that the relative lengths of sex chromosomes in the two species were different from each other. The sex-chromosome of Ch. （M.） aethalinus is located at the fifth position and its relative length is 8.33% whereas that of Ch. （M.） chinensis is at the eighth position and its relative length is 5.53%. These results show that significantly different genetic features exist for the two species of Chorthippus.
基金Supported by the State key Subjecet of Botany at Hainan University (071001)the Malor Scientific Research Protect of Hainan Province, China(20080137)~~
文摘[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.
基金supported by the National Natural Science Foundation of China (31060291)
文摘A new species, Hedotettix nujiangensis Zheng sp. nov., is described. The chromosome complement of H. nujiangensis consists of 2n （♂） = 13. Sex determination is XO. All chromosomes are telocentric （T） and the sex chromosome is the fourth element in size. Type specimens are deposited at Southwest Forestry University.
基金funded by the Special Fund for Agro-scientific Research in the Public Interest(201003079)Special Fund for Basic Scientific Research Project in the Central Scientific Research Institutes(Institute of Grassland Research of CAAS)(1610332014012)+1 种基金National Natural Science Foundation of China(41301049)the Ministry of Science and Technology of the Republic of China(2013DFR30760)
文摘The chromosome karyotypes of Chorthippus albomarginatus （De Geer） which represents the genus Chorthippus Fieber and Euchorthippus unicolor （Ikonnikov） which represents the genus Euchorthippus Tarbinsky were compared. The chromosome numbers for these two species fit 2n （♂） = 17 = 16 ＋ XO, in which five pairs of autosomal and sex chromosomes are terminal chromosomes, and the other three pairs of autosomes are metacentric chromosomes. This similarity between the two genera indicates that they have a close phylogenetic relationship. However, these two species can be distinguished by their different chromosome formula （CF） and the relative sex chromosome length （RL）. The CF in C. albomarginatus is K （2n,♂） = 6m ＋ 11t = 8L ＋ 4M ＋ 4S ＋ XO, whereas that ofE. unicolor is K （2n, ♂） = 6m ＋ 11t = 8L ＋ 6M ＋ 2S ＋ XO. The relative lengths of sex chromosomes in the two species are different. The sex-chromosome of C. albomarginatus is located at the ninth position and its relative length is 2.96% whereas that ofE. unicolor is at the eighth position and its relative length is 4.26%. These results show that significantly different genetic features exist for the two genera.
文摘The chromosomes of three odor frogs, Luetuosae- group of Rana were analyzed by conventional, as well as Obanding and silver staining techniques. The three species here examined all had 26 chromosome karyotypes encompassing 5 large and 8 small homologous pairs. Differences among them were found in gross shapes of chromosomes and positions of secondary constrictions. Generally speaking, karyotypes of R. andersonii and R. grahami resembled each other, while the karyotype of R. tiannanensis differed from the former two in several respects.Analyses of karyotypes by C-banding technique indicated that, centromeric areas of every chromosome and interstitial parts of some chromosomes of each species were hetero-chromatinized, and differences of distribution of heterochromatin were found among species. In early metaphase plates of R. andersonii, much more heterochromatinized areas were observed, and when it reached late metaphase, the numbers of heterochromatin sections in each chromosome pair reduced to a limited level, e. g. centromeres and a few interstitial parts.The active nucleolar organizer regions (NORs) were localized in long arms of pair No. 10, in connection with secondary constrictions for R. andersonii and R. grahami as in other odor frogs previously reported, but in the long arm of pair No. 6 for R. tiannanensis.The cytogenetic and taxonomic implications of the findings were discussed based on comparisons with each other, and with published literature.
文摘We examined mitotic chromosomes of three newly found members of the Drosophila obscura species group,D.luguensis,D.dianensis and D.limingi from Yunnan and compared their karyotypes to those of respective related species.D.luguensis possessed a diploid number of 12 chromosomes,comprising 3 pairs of metacentrics (V-shaped),2 pairs of acrocentrics (rod-like) and 1 pair of micro-chromosomes (dot-like).Both the X and Y were metacentrics.D.dianensis and D.limingi possessed a diploid number of 10 chromosomes,comprising 1 pair of large V-shaped,1 pair of moderate-sized V-shaped,2 pairs of submetacentric (J-shaped) chromosomes,and 1 pairs of dot-like chromosomes,with their X chromosomes were J-shaped and Y chromosomes were short rod-like ones.Based upon the results of chromosomal comparisons,geographic information of the sinobscura subgroup,as well inter-specific phylogenetic relationship deduced elsewhere,D.luguensis was considered to retain an ancestral form of the karyotype within this subgroup.The present observations have also implied that D.sinobscura (2n=12:2V,1J,2R,1D) might have derived from a sub-lineage of a pre-“sinobscura-hubeiensis” lineage by pericentric inversion of moderate-sized metacentric autosomes and D.hubeiensis (2n=10:4V,1D) from another sub-lineage of the same lineage by centric fusion of 2 pairs of acrocentric autosomes.Similar chromosomal changes of centric fusion,centric fission and/or pericentric inversion might have occurred during the course of species divergences between D.dianensis and D.subsilvestris (2n=12:3V,2R,1D),an European member closely related to D.dianensis,and between D.limingi and D.tsukubaensis (2n=12:3V,2R,1D),an East Asian member near to D.limingi.
基金Supported by Scientific Research Foundation for Doctors of Qufu Normal University~~
文摘[Objective] This study aimed to compare the similarities and dissimilarities in karyotypes of purple and white onions,so as to lay foundation for further studying its genetic improvement and breeding more onion varieties.[Method] Sections of root-tip cells were prepared by normal wafer method.Photomicrography was adopted to produce the high-quality chromosome specimen.Finally,karyotype analysis was conducted using Adobe Photoshop.[Result] Chromosome karyotype analysis on the root-tip showed that the chromosome number of both purple and white onions was 2n=2x=16.Among the 16 chromosomes of both the onions,no satellite chromosome was found.By measuring and calculating,it was found that the karyotype of the studied white union belonged to the lowly evolutional 2A type,and its karyotype formula was 2n=2x=16=14 m ＋2St,with the karyotype asymmetry coefficient of 58.67%.No obvious centromere was observed in the sections of purple onion.[Conclusion] The karyotypes of white and purple unions are similar and we can infer from this fact that the reason of different characters between both the onions is not due to the chromosome aberration.This karyotype formula and asymmetrical karyotype coefficient have some reference values on the identification of onion variety resources.
基金supported by the National Natural Science Foundation of China (30571321)the Natural Science Foundation of Beijing, China(6082009)
文摘Elytrigia Desv. is widely distributed throughout the world and is represented with species of various levels of ploidy including diploids, tetraploids, hexaploids, octaploids, and decaploids. The distribution pattern of these ploidy levels, however, is not well-defined. In this study, the levels of ploidy for 64 accessions of Elytrigia from 25 countries were determined with microscopic procedures. The results showed that accessions of E. intermedia and E. repens were grouped into three distinct levels of ploidy including diploids, tetraploids and hexaploids. For E. elongata, E. pontica, and E. caespitosa, it was found that two ploidy levels presented, and only one ploidy level was in those of E. hybrid, E. pycnantha, E. pungens, E. juncea, and E. alatavica. Karyotype analysis indicated that the karyotype formula of diploid, tetraploid and hexaploid of E. intermedia was 2n = 2x = 14 = 6m ＋ 6sm ＋ 2st, 2n = 4x = 28 = 2M ＋ 10m ＋ 16sm and 2n = 6x = 42 = 4M ＋ 18m ＋ 20sin, respectively. Furthermore, the karyotype formula of three germplasms in tetraploid of E. intermedia was 2n=4x=28 =2M＋ 10m＋ 16sm, 2n=4x=28=4M＋22m （sat）＋2sm and 2n=4x=28 =4M＋ 12m＋ 12sm （sat）, which were not completely uniform. Therefore, it could be suggested that the studies about chromosome constitution would be helpful for the detail understanding of the diversity of germplasm resource in Elytrigia and promoting the utilization in the crop molecular breeding.
基金supported by the National Natural Science Foundation of Qinghai Province (2015-ZJ-903)the National Natural Science Foundation of China (31072075)
文摘The karyotypes of 27 individuals of Elymus nutans from eight wild populations in the Qinghai Plateau were analyzed using sequential FISH and GISH. High FISH pattern polymorphism and karyotype variation were detected within and among populations. The chromosome variations were mainly characterized as repeat deletions and amplifications along with inter-genomic translocations. The chromosomes of the St and Y genomes demonstrated higher polymorphism than those of the H genome. Six different intergenomic translocations were identified in 33.3% of individuals; type Ⅰ and Ⅱ translocations were detected with higher frequency. Further analysis revealed that type Ⅰ and Ⅱ translocations were distributed in different geographic regions. The origin of high karyotype variation of E. nutans in the Qinghai plateau is further discussed.
文摘The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants.
