This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospe...This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospecies of filamentous endophytes were isolated of which twelve isolates were successfully identified to the species level.These isolates were identified as Aspergillus versicolor,Aspergillus sp.,Chaetosphaeria sp.,Cladosporium cladosporioides,Hypocrea lixii,Microascus murinus,and Trichoderma harzianum.The identified twelve isolates were selected to screen in vitro for their plant growth-promoting characteristics,and evaluated in vivo for their beneficial effects on seedling vigor and early seedling growth.Isolate PPL14 produced the highest IAA at 55.5μg ml^(-1)and M.murinus PPL10 produced the highest amount of IAA at 3.73μg IAA mg^(-1)dry mycelia wt.The seedling vigor assay and in vivo plant growth promotion bioassay indicated overall positive effects of culture filtrate(CF)application of the endophyte isolates.Rice seeds and seedlings grown in aseptic condition and treated with endophyte CFs displayed significantly enhanced levels of germination,seedling vigor,shoot,root,and total plant growth,and biomass compared to non-treated control.Other plant growth-promoting characteristics were also studied including phosphate solubilization,siderophore production,ammonia production,and catalase activity.This study supports the potential use of fungal endophytes as bio-inoculants for plant growth promotion and enhancement of nutrient assimilation of agriculturally important crops.展开更多
[Objectives]This study was conducted to select multi-resistant rice varieties in Hainan.[Methods]Seven new rice germplasms were identified by naturally induced disease nursery,field spraying and artificial inoculation...[Objectives]This study was conducted to select multi-resistant rice varieties in Hainan.[Methods]Seven new rice germplasms were identified by naturally induced disease nursery,field spraying and artificial inoculation.[Results]The restorer line Haihui 1558 was resistant to the dominant races ZB and ZC of rice blast and Tryporyza incertulas,and moderately resistant to pathotype IV of bacterial blight.The restorer line Haihui 227 was resistant to pathotype IV of bacterial blight and T.incertulas.Red rice Hainonghong 1 was resistant to pathotype IV of bacterial blight,and moderately resistant to the dominant races ZB and ZC of rice blast.Black glutinous rice Haifengheinuo 2 was moderately resistant to pathotypes IV of bacterial blight and T.incertulas.[Conclusions]The study provides reference for utilization of rice breeding on resistance to diseases and pests.展开更多
基金This project is supported by a Junior Thesis Fellowship Grant awarded to the authors by the Philippine Rice Research Institute(PhilRice).
文摘This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospecies of filamentous endophytes were isolated of which twelve isolates were successfully identified to the species level.These isolates were identified as Aspergillus versicolor,Aspergillus sp.,Chaetosphaeria sp.,Cladosporium cladosporioides,Hypocrea lixii,Microascus murinus,and Trichoderma harzianum.The identified twelve isolates were selected to screen in vitro for their plant growth-promoting characteristics,and evaluated in vivo for their beneficial effects on seedling vigor and early seedling growth.Isolate PPL14 produced the highest IAA at 55.5μg ml^(-1)and M.murinus PPL10 produced the highest amount of IAA at 3.73μg IAA mg^(-1)dry mycelia wt.The seedling vigor assay and in vivo plant growth promotion bioassay indicated overall positive effects of culture filtrate(CF)application of the endophyte isolates.Rice seeds and seedlings grown in aseptic condition and treated with endophyte CFs displayed significantly enhanced levels of germination,seedling vigor,shoot,root,and total plant growth,and biomass compared to non-treated control.Other plant growth-promoting characteristics were also studied including phosphate solubilization,siderophore production,ammonia production,and catalase activity.This study supports the potential use of fungal endophytes as bio-inoculants for plant growth promotion and enhancement of nutrient assimilation of agriculturally important crops.
基金Supported by Key R&D plan of Hainan Province(ZDYF2017068)Special Fund for Technology Development Research of Hainan Provincial Scientific Research Institutes(KYYS-2015-11)Earmarked Fund for China Agriculture Research System(CARS-01-89)。
文摘[Objectives]This study was conducted to select multi-resistant rice varieties in Hainan.[Methods]Seven new rice germplasms were identified by naturally induced disease nursery,field spraying and artificial inoculation.[Results]The restorer line Haihui 1558 was resistant to the dominant races ZB and ZC of rice blast and Tryporyza incertulas,and moderately resistant to pathotype IV of bacterial blight.The restorer line Haihui 227 was resistant to pathotype IV of bacterial blight and T.incertulas.Red rice Hainonghong 1 was resistant to pathotype IV of bacterial blight,and moderately resistant to the dominant races ZB and ZC of rice blast.Black glutinous rice Haifengheinuo 2 was moderately resistant to pathotypes IV of bacterial blight and T.incertulas.[Conclusions]The study provides reference for utilization of rice breeding on resistance to diseases and pests.
