Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed ...Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed as the second batch of national all-in-one tourism demonstration zones,the establishment of al-in-one tourism puts forward higher requirements for the planning and promotion of rural landscape in Yuan'an County.By analyzing the status quo of rural landscape construction through landscape cultural characteristics,landscape differentiation and landscape management mechanism,combined with the current style of Luyuan Village in Yuan'an County,the paper discusses the promotion strategy and implementation approach of rural landscape in the perspective of all-region tourism from natural ecological landscape,settlement life landscape and economic production landscape,in order to bring new thinking for the improvement of rural landscape in China.展开更多
Coxsackievirus A16(CVA16) is one of major pathogens of hand, foot and mouth disease(HFMD) in children. Long non-coding RNAs(Inc RNAs) have been implicated in various biological processes,but they have not been associa...Coxsackievirus A16(CVA16) is one of major pathogens of hand, foot and mouth disease(HFMD) in children. Long non-coding RNAs(Inc RNAs) have been implicated in various biological processes,but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of Inc RNAs of normal and CVA16 infected rhabdomyosarcoma(RD)cells using RNA-Seq to investigate the functional relevance of Inc RNAs. We showed that a total of 760 Inc RNAs were upregulated and 1210 Inc RNAs were downregulated. Out of these dysregulated Inc RNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were s RNA host Inc RNAs and 0.05% were enhancer. Six dysregulated Inc RNAs were validated by quantitative PCR assays and the secondary structures of these Inc RNAs were projected. Moreover, we conducted a bioinformatics analysis of an Inc RNAs(ENST00000602478) to elucidate the diversity of modification and functions of Inc RNAs. In summary, the current study compared the dysregulated Inc RNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and Inc RNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD.展开更多
文摘Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed as the second batch of national all-in-one tourism demonstration zones,the establishment of al-in-one tourism puts forward higher requirements for the planning and promotion of rural landscape in Yuan'an County.By analyzing the status quo of rural landscape construction through landscape cultural characteristics,landscape differentiation and landscape management mechanism,combined with the current style of Luyuan Village in Yuan'an County,the paper discusses the promotion strategy and implementation approach of rural landscape in the perspective of all-region tourism from natural ecological landscape,settlement life landscape and economic production landscape,in order to bring new thinking for the improvement of rural landscape in China.
基金supported by the National Natural Sciences Foundation of China(No.81171577,81371790,81371422 and 81171127)Major AIDS and Viral Hepatitis and Other Major Infectious Disease Prevention and Control project of China(2014ZX10001003)+1 种基金the Fundamental Research Funds for the Central Universities of Chinathe Translational Medical Research Fund of Wuhan University School of Medicine
文摘Coxsackievirus A16(CVA16) is one of major pathogens of hand, foot and mouth disease(HFMD) in children. Long non-coding RNAs(Inc RNAs) have been implicated in various biological processes,but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of Inc RNAs of normal and CVA16 infected rhabdomyosarcoma(RD)cells using RNA-Seq to investigate the functional relevance of Inc RNAs. We showed that a total of 760 Inc RNAs were upregulated and 1210 Inc RNAs were downregulated. Out of these dysregulated Inc RNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were s RNA host Inc RNAs and 0.05% were enhancer. Six dysregulated Inc RNAs were validated by quantitative PCR assays and the secondary structures of these Inc RNAs were projected. Moreover, we conducted a bioinformatics analysis of an Inc RNAs(ENST00000602478) to elucidate the diversity of modification and functions of Inc RNAs. In summary, the current study compared the dysregulated Inc RNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and Inc RNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD.
