In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2–3 mm)under three different photoperiods(L...In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2–3 mm)under three different photoperiods(L:D=12:12,14:10 and 10:14),temperature(15℃(LT),25℃(MT)and 30℃(HT))and light intensity(100,200 and 400μmol m^(−2)s^(−1),noted as LL,ML and HL,respectively)conditions.Then the maturation time,spore release number and chlorophyll fluorescence were analyzed.The results suggested that:1)The spore maturation time was accelerated by higher temperature or higher light intensity from 62 h to 36 h,and changes in day length accelerated the spore maturation to a certain extent as compared with 12:12 light/dark cycle;2)Higher light intensity significantly decreased the chlorophyll fluorescence(Fv′/Fm′,NPQ,rETRmax andα)of the mature reproductive segment under 30℃with 12:12 light/dark cycle.But when in the other photoperiods(10:14 and 14:10 conditions),the inhibitory effects of high light intensity were alleviated significantly;3)The optimum condition for the spore maturation and release was 12:12 light/dark cycle,25℃,400μmol m^(−2)s^(−1),with both shorter and longer photoperiod reducing the spore release number;4)Higher light intensity significantly increased the spore release number under 25℃,but these effects were alleviated by 30℃treatment.This study is the first attempt to elucidate the coincidence effects of photoperiod,temperature and light intensity on the reproduction of Ulva,which would help to reveal the mechanism of the rapid proliferation of green tide.展开更多
Saccharina japonica gametophytes can survive a long period under unfavorable environmental conditions,while they also delay in growth and/or reproduction.Although the reproduction in delayed gametophyte of S.japonica ...Saccharina japonica gametophytes can survive a long period under unfavorable environmental conditions,while they also delay in growth and/or reproduction.Although the reproduction in delayed gametophyte of S.japonica was known to be strongly influenced by light intensity,light quality,and photoperiod,no previous studies have evaluated their interactive effects on gametogenesis.To evaluate these effects,we used an orthogonal experiment to expose delayed gametophytes of S.japonica to different light intensities,light qualities,and photoperiods for 12 days.The results showed that changes in light intensity rather than light quality and photoperiod significantly affected the relative growth rates of the delayed gametophytes.Blue light had the greatest promotion on reproduction rate.The optimal light conditions in the early vegetative growth phase in gametogenesis induction for the delayed gametophytes were at 60–80μmol photons m^(−2) s^(−1) with daylength of 12 or 16 hours under white or blue light.When the delayed gametophytes were maintained in a constant light condition from delayed state to gametogenesis,the beneficial photoperiods for vegetative growth and reproductive rate were both 16L(16 hours of light):8D(8 hours of dark).However,when the delayed S.japonica gametophytes achieve the optimal growth state during the first 6 days and then they were cultured at different light conditions for the following 6 days,the reproduction rate increased as the daylength decreased and attained a peak value in group of 8L:16D photoperiod,indicating that photoperiod adjustment at the transition period is crucial in the gametogenesis induction process of delayed gametophyte of S.japonica.展开更多
In this study, an effective environment for Chlorella vulgaris growth is sought after. As a substitute source of food and feed, increasing the cell density of Chlorella culture is one of the keys to ensuring sustainab...In this study, an effective environment for Chlorella vulgaris growth is sought after. As a substitute source of food and feed, increasing the cell density of Chlorella culture is one of the keys to ensuring sustainability. It can be showed from different studies that optimum light intensity and pH could increase cell density. In this study, the effects of light and pH on the growth rate of C. vulgaris were observed in photobioreactor. A specific wavelength (682 nm) was determined by UV-Vis Spectrophotometry to carry out the further analysis. The light intensities were set at 7409, 9261 and 11,113 lux;pH values were set at 7, 8 and 9 respectively. The experimental results depicted the light intensity of 9261 lux as the best due to the higher number of cells (48.56 × 10<sup>6</sup> cells/mL) obtained using this intensity. In terms of pH, without pH control, cell numbers were found to be highest under the light intensity of 9261 lux. When pH was controlled, it was found that under the optimum light intensity, pH control between 7.0 and 7.5 was the optimum range for the growth of C. vulgaris. Moreover, this method of study may possibly be a promising source of low cost culture for Chlorella vulgaris.展开更多
Under natural conditions, plants constantly encounter various biotic and abiotic factors, which can potentially restrict plant growth and development and even limit crop productivity. Among various abiotic factors aff...Under natural conditions, plants constantly encounter various biotic and abiotic factors, which can potentially restrict plant growth and development and even limit crop productivity. Among various abiotic factors affecting plant photosynthesis, light serves as an important factor that drives carbon metabolism in plants and supports life on earth. The two components of light(light quality and light intensity) greatly affect plant photosynthesis and other plant's morphological, physiological and biochemical parameters. The response of plants to different spectral radiations and intensities differs in various species and also depends on growing conditions. To date, much research has been conducted regarding how different spectral radiations of varying intensity can affect plant growth and development. This review is an effort to briefly summarize the available information on the effects of light components on various plant parameters such as stem and leaf morphology and anatomy, stomatal development, photosynthetic apparatus, pigment composition, reactive oxygen species(ROS) production, antioxidants, and hormone production.展开更多
Central serous chorioretinopathy(CSC) is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains obsc...Central serous chorioretinopathy(CSC) is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains obscure. In this paper, we hypothesized that CSC may result from a response of choroidal vessels to an acute increase in the environmental light intensity leading to a focal leakage from the choroidal vessels.High levels of glucocorticoids, in our proposed model,may cause persistence rather than initiation of the focal leakage, probably by suppressing the synthesis of collagen and extracellular matrix components and inhibiting fibroblastic activity.展开更多
Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of bas...Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.展开更多
Bound extracellular polymeric substances(bEPS)play an important role in the proliferation of Microcystis.However,the understanding of bEPS characterization remains limited.In this study,threedimensional fluorescence e...Bound extracellular polymeric substances(bEPS)play an important role in the proliferation of Microcystis.However,the understanding of bEPS characterization remains limited.In this study,threedimensional fluorescence excitation-emission matrix(3D-EEM)spectroscopy and zeta potentiometer were used to characterize the loosely bound EPS(LB-EP S)and tightly bound EPS(TB-EPS)from two dominant Microcystis morphospecies from Taihu Lake(China)at different light intensities.Physiochemical analysis showed that the growth and TB-EPS or bEPS contents in Microcystis aeruginosa were higher than those in Microcystisfl os-aquae at each light intensity.The 3D-EEM contour demonstrated that the intensities of peak B(tryptophan-like substances)in the TB-EPS from M.aeruginosa were stronger than those from M.flosaquae when the light intensity was higher than 10μE/(m^(2)·s).Zeta potential analysis showed that the absolute values of the zeta potential of TB-EPS in the two species both increased with rising light intensity,except those of TB-EPS in M.aeruginosa at 105μE/(m^(2)·s).Moreover,the absolute values of the zeta potential of M.aeruginosa were higher than tho se of M.flos-aquae at each light intensity.All these re sults indicated that M.aeruginosa may more quickly proliferate than M.flos-aquae through increased negative charges,bEPS contents.growth.and tryptophan-like substance contents at certain light intensities.展开更多
The active compounds in herb drugs are mainly secondary metabolites, which are greatly influenced by external conditions. Particularly, light intensity has a great influence on the photosynthesis and accumulation of s...The active compounds in herb drugs are mainly secondary metabolites, which are greatly influenced by external conditions. Particularly, light intensity has a great influence on the photosynthesis and accumulation of secondary metabolites. In this study, the light intensity was changed, and the influence of the light intensity on leaf photosynthetic characteristics, antioxidant enzyme activity and flavone contents of Lithocarpus litseifoliusp (Hance) Chun. was discussed. The results showed that (1) L. litseifolius is a typical heliophyte. As the light intensity decreased, the contents of chlorophyll a (Chl a), chlorophyll b (Chl b) and total chlorophyll (Chl a + b) all increased. However, the Chl a/b ratio gradually decreased. The daily variation of net photosynthetic rate changed from a double-peak curve under natural light to single-peak curve under 20% full light two shading nets. (2) As the light intensity decreased, the SOD and POD activities and the soluble proteins content decreased in the leaves. In contrast, the malondialdehyde content increased. (3) The flavone content was the highest in leaves at one shading nets 60% of full light, and the lowest occurred in leaves at two shading nets 20% of full light. A comprehensive analysis indicated that an appropriate shading could induce shade tolerance in the leaves of L. litseifolius, which promoted the accumulation of flavonoids. L. litseifolius should be planted in the lower position of sunny slope or the upper position of shady slope to increase the content of flavone in the leaves.展开更多
Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fade...Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fades away in summer and winter.Light intensity is one of the most important factors affecting leaf color along the seasons.In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves,Ananas comosus var.bracteatus was grown under full sunlight,50%shade and 75%shade for 75 days to evaluate the concentration of pigments,the color parameters(values L^(*),a^(*),b^(*))and the morpho-anatomical variations of chimeric leaves.The results showed that a high irradiance was beneficial to keep the chimeric leaves red.However,prolonged exposure to high irradiance caused a damage,some of the leaves wrinkled and even burned.Shading instead decreased the concentration of anthocyanin and increased the concentration of chlorophyll,especially in the white marginal part of the leaves.Numerous chloroplasts were observed in the mesophyll cells of the white marginal part of the chimeric leaves under shading for 75 days.The increase in chlorophyll concentration resulted in a better growth of plants.In order to balance the growth and coloration of the leaves,approximately 50%shade is suggested to be the optimum light irradiance condition for Ananas comosus var.bracteatus in summer.展开更多
Light is crucial to the uniform production of high-quality fruit since it is the driving force for leaf photosynthesis and hence overall plant nutrition.The objective of this study was to evaluate the relationship bet...Light is crucial to the uniform production of high-quality fruit since it is the driving force for leaf photosynthesis and hence overall plant nutrition.The objective of this study was to evaluate the relationship between the distribution of relative light intensity in the peach tree(Prunus persica)canopy and the yield and texture of peach fruits.The canopy of 7-year-old‘Qiuyan’was divided into cubical volumes and the relative light intensity distribution was measured for each cube,along with yield distribution and fruit textural properties at different growing times.The relative light intensity decreased gradually from outside to inside the canopy and from top to bottom.The yield distribution and the relative light intensity were clearly correlated.The percentage of the canopy receiving<30%relative light intensity was 49.07%in May,56.02%in June,and 58.95%in July,whereas the percentage receiving>80%relative light intensity was 32.72%,17.28%,and 10.96%,respectively.Consistent with this,peaches were found in the upper and middle portions of the canopy,within 1.5-3.0 m of the top.The regression equation showed that fruit texture index correlated significantly with relative light intensity.Relative light intensity is the main factor affecting peach yield and texture and must be above 41.83%for good peach quality.Orchardists should carefully plan summer pruning strategies to adjust the number and spatial distribution of branches accordingly.展开更多
Light is an essential natural factor of fish growth and development.A light regime in indoor aquaculture is critical for sustainable fish production.This research aimed to investigate the effect of light intensity and...Light is an essential natural factor of fish growth and development.A light regime in indoor aquaculture is critical for sustainable fish production.This research aimed to investigate the effect of light intensity and photoperiod on the growth performance and stress response of Nile tilapia(Oreochromis niloticus),which is an important commercial species,in a recirculating aquaculture system(RAS).Fingerlings with an average weight of 5±0.9 g were randomly assigned to nine 1.5 m3 tanks with 145 fish in each tank,cultured under different light intensities(1000,2000,and 3000 lx)and photoperiods(12L:12D,18L:6D,24L:0D),and fed to apparent satiation twice a day for 160 days.The growth of tilapia subjected to 2000 lx was significantly better than that exposed to 1000 and 3000 lx(P<0.05)as indicated by the final weight(351.17±10.59 g),growth efficiency(0.77±0.26),specific growth rate(2.65%±0.21%per day)and feed conversion coefficient ratio(1.30±0.36).No significant difference was observed in the growth rate among different photoperiod groups(P>0.05).Light intensity and photoperiod manipulation did not cause a significant chronic stress response in tilapia.This study demonstrated that light intensity,especially at 2000 lx,and photoperiod manipulation could stimulate the growth of tilapia in the RAS and significantly affect economic profitability.This study also served as an important reference for tilapia indoor aquaculture.展开更多
Murray cod(Maccullochella peelii)is a freshwater percichthyid fish that has a high market value and potential for culture in recirculating aquaculture systems(RASs).Illumination is an important environmental factor th...Murray cod(Maccullochella peelii)is a freshwater percichthyid fish that has a high market value and potential for culture in recirculating aquaculture systems(RASs).Illumination is an important environmental factor that affects the growth and physiological condition of fishes.A comprehensive understanding of the relationship between the light factors and the growth,nutrient composition and stress response of juvenile Murray cod in RAS is important to achieve satisfactory theoretical and practical aquaculture performance.Juvenile Murray cod were randomly assigned to nine RAS tanks with a volume of 1.5 m^(3),with each tank containing 120 fishes(3.5±0.5g).The fish were cultured for 120 days under different light intensities(1200,2400,and 3600 lx)and photoperiods(12L:12D,18L:6D and 24L:0D).The results showed that the final weight and feed conversion ratio of Murray cod under a light intensity of 1200 lx were significantly better than those under 3600 lx(P<0.05).The serum total protein and globulin were higher at the light intensity of 1200lx,compared to other light intensities.The blood urea nitrogen level improved with increasing light intensity at LD12:12 and LD18:6 of photoperiod,as well as the superoxide dismutase level improved significantly from LD18:6 to LD24:0 of photoperiod.The findings indicated that the optimal light condition to enhance growth at juvenile stage is 1200 lx of the light intensity and LD18:6 of the photoperiod.展开更多
Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydro...Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydroponically grown arugula was investigated by using light-emitting diodes(LEDs)to light the hydroponically grown arugula for a reference for industrialized arugula production.The dynamic demands of arugula for LI in the seedling stage,initial growth stage and vigorous growth stage were tested under two light quality conditions including a red/blue light ratio of 7:1 and a light/dark photoperiod of 12 h/12 h.Then,the curves of variable LI-induced changes in the growth indices of arugula in different development periods were drawn.Next,the influence of variable LI on the growth characteristics and nutritional quality of arugula was investigated by measuring the dry/fresh weight ratio,chlorophyll content,vitamin C content and soluble protein content.Variable LI significantly increased the height,stem diameter,leaf width,dry/fresh weight ratio,chlorophyll content and soluble protein content of arugula plant.Plant height,stem diameter,dry/fresh weight ratio,chlorophyll content and soluble protein content were the highest in the group exposed to LI of 200,300 and 300μmol•m^(-2)•s^(-1)during the seedling stage,initial growth stage and vigorous growth stage,respectively.The greatest leaf width was achieved at LI of 100,250 and 350μmol•m^(-2)•s^(-1),respectively.High intensity LI markedly repressed the synthesis of vitamin C.展开更多
The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of ina...The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.展开更多
The current climate and energy crisis urgently needs solar cells with efficiencies above the 29% single junction efficiency bottleneck.Silicon/perovskite tandem solar cells are a solution,which is attracting much atte...The current climate and energy crisis urgently needs solar cells with efficiencies above the 29% single junction efficiency bottleneck.Silicon/perovskite tandem solar cells are a solution,which is attracting much attention.While silicon/perovskite tandem cells in 2-terminal and 4-terminal configurations are well documented,the three-terminal concept is still in its infancy.It has significant advantages under low light intensities as opposed to concentrated sunlight,which is the critical factor in designing tandem solar cells for low-cost terrestrial applications.This study pre-sents novel studies of the sub-cell performance of the first three-terminal perovskite/silicon selective band offset barrier tandem solar cells fabricated in an ongoing research project.This study focuses on short circuit current and operating voltages of the subcells under light intensities of one sun and below.Lifetime studies show that the perovskite bulk carrier lifetime is insensitive to illumination,while the silicon cell's lifetime decreases with decreasing light intensity.The combination of perovskite and silicon in the 3T perovskite-silicon tandem therefore reduces the sensitivity of V_(OC) to light intensity and maintains a relatively higher V_(OC) down to low light intensities,whereas silicon single-junction cells show a marked decrease.This technological advantage is proposed as a novel advantage of three-terminal perovkite/silicon solar cells for low light intensities of one sun or less.展开更多
Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations ...Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.展开更多
Although the distributions of foliage and light play major roles in various forest functions,accurate,nondestructive measurement of these distributions is difficult due to the complexity of the canopy structure.To eva...Although the distributions of foliage and light play major roles in various forest functions,accurate,nondestructive measurement of these distributions is difficult due to the complexity of the canopy structure.To evaluate the foliage and light distributions directly and nondestructively in a mature oak stand,we used the cube method by dividing the forest canopy into small cubes(50 cm per side)and directly measured leaf area density(LAD,the total one-sided leaf area per unit volume,i.e.,cube)and relative irradiance(RI)within each cube.The distribution of LAD and of RI was highly heterogeneous,even at the same canopy height.This heterogeneity reflected the presence of foliage clusters associated with multiple forking branches.The relationship between cumulative LAD at the canopy surface and average RI followed the Beer-Lambert law.The mean light extinction coefficient(K)was 0.32.However,K was overestimated by more than double(0.80)when calculated based on the classical method using RI at the forest floor.This overestimation was caused by the lower RI due to light absorption by nonleaf plant parts below the canopy.Our findings on the complex foliage and light distributions in canopy layers should help improve the accuracy of RI and K measurements and thus more accurate predictions of environmental responses and forest functions.展开更多
Objective To evaluate the effect of intense pulsed light(IPL)in the treatment of chronic hordeolum.Methods Patients with chronic hordeolum who underwent IPL treatment were enrolled in this study.According to the sever...Objective To evaluate the effect of intense pulsed light(IPL)in the treatment of chronic hordeolum.Methods Patients with chronic hordeolum who underwent IPL treatment were enrolled in this study.According to the severity of hordeolum,the patients were treated with IPL 3 to 5 times.Patients’satisfaction and visual analog scale scores for ocular discomfort symptoms before and after treatment were collected.The number,congestion,long diameter,short diameter and area of nodules were also recorded and measured.Finally,eyelid margin signs,meibum quality,meibomian gland expressibility,meibomian gland dropout,tear meniscus height,and corneal fluorescein staining were scored.Results 20 patients were enrolled in this study.The eyelid margins were congestive and swollen,with blunt rounding or irregularity.The meibum was cloudy or toothpaste-like.The meibomian gland expressibility,meibomian gland dropout and tear meniscus height were reduced.The cornea showed scattered fluorescein staining.After treatment,score of visual analog scale,congestion and size of nodules were significantly reduced.Eyelid margin signs,meibum quality,meibomian gland expressibility,tear meniscus height and corneal fluorescein staining scores were improved.Meibomian gland dropout had no significant change.No side effects occurred during treatment.Conclusions IPL is beneficial for the treatment of chronic hordeolum.展开更多
Three Gracilariopsis lemaneiformis strains,including wild type and high-temperature-resistant cultivars 981 and 2007,were studied with the changes in their photosynthetic pigment contents and related gene transcriptio...Three Gracilariopsis lemaneiformis strains,including wild type and high-temperature-resistant cultivars 981 and 2007,were studied with the changes in their photosynthetic pigment contents and related gene transcription levels under different light intensities(10,60,100,and 200μmolm^(−2)s^(−1)).The three G.lemaneiformis strains had the following photosynthetic pigments with high-to-low contents:phycoerythrin(PE),phycocyanin(PC),allophycocyanin(APC),and chlorophyll a(Chl a).Among the three strains,cultivar 981 had the highest PE content,followed by cultivar 2007.The PC and APC contents were similar among the three strains,but they were higher in cultivars 981 and 2007 than in the wild type.The Chl a contents in the three G.lemaneiformis strains were equal.A low light intensity(10μmolm^(−2)s^(−1))promoted photosynthetic pigment accumulation in G.lemaneiformis and improved the relative PE gene transcription(peA and peB)in a short period(≤6 d).A high light intensity decreased the PE content.PebA and PebB,which catalyzed phycoerythrobilin synthesis,showed no compensatory upregulation at a low light intensity among the strains except for the wild type.At a high light intensity,transcription levels of pebA and pebB in the three strains were upregulated.This study provided an experimental basis for elucidating the photosynthesis of G.lemaneiformis.As key genes of algal growth,photo-synthesis-related genes served as useful gene markers for screening elite varieties with good traits in breeding.Cultivar 2007 was superior to cultivar 981 in terms of maintaining high pigment levels in a wide range of light intensities,which is the most suitable for aquaculture.展开更多
Harmful algae bloom constitutes a major problem facing water bodies particularly fresh and marine water system. <i>Microcystis aeruginosa</i> represents a major causative organism found in the water. Light...Harmful algae bloom constitutes a major problem facing water bodies particularly fresh and marine water system. <i>Microcystis aeruginosa</i> represents a major causative organism found in the water. Light plays a major role in the growth and variation of <i>M. aeruginosa</i> in water. What is still inadequate, is the data on the effects of different light conditions on the growth of <i>Microcystis aeruginosa</i>. In this study, two strains of <i>Microcystis aeruginosa</i> PCC 7806 (toxic strain) and PCC 7005 (non-toxic strain) were exposed to varying light intensities at 0, 30, 60, 120, 300, 350 μMol photon m<sup>-2</sup>·S<sup>-1</sup> to measure their growth and pigmentation. This study observed similarities in growth and pigmentation between toxic strain (PCC 7806) and non-toxic (PCC 7005) strain. Also, at short exposure to light, Toxic strain was seen to be better tolerant of light than non-toxic strain, with non-toxic strain showing better ability to recover from light stress after twenty days of culture. These findings suggest that Toxic strain (PCC 7806) and Non-toxic strain (PCC 7005) of <i>M. aeruginosa</i> respond similarly with respect to growth but differ in their light retention capacity over time and this could be useful in predicting the possible conditions of algal bloom.展开更多
基金supported by the Natural Science Foundation of Zhejiang Province(No.LY23D060003)the Key Program of Science and Technology Innovation in Ningbo(2021Z114,2023Z118)sponsored by K.C.Wong Magna Fund in Ningbo University.
文摘In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2–3 mm)under three different photoperiods(L:D=12:12,14:10 and 10:14),temperature(15℃(LT),25℃(MT)and 30℃(HT))and light intensity(100,200 and 400μmol m^(−2)s^(−1),noted as LL,ML and HL,respectively)conditions.Then the maturation time,spore release number and chlorophyll fluorescence were analyzed.The results suggested that:1)The spore maturation time was accelerated by higher temperature or higher light intensity from 62 h to 36 h,and changes in day length accelerated the spore maturation to a certain extent as compared with 12:12 light/dark cycle;2)Higher light intensity significantly decreased the chlorophyll fluorescence(Fv′/Fm′,NPQ,rETRmax andα)of the mature reproductive segment under 30℃with 12:12 light/dark cycle.But when in the other photoperiods(10:14 and 14:10 conditions),the inhibitory effects of high light intensity were alleviated significantly;3)The optimum condition for the spore maturation and release was 12:12 light/dark cycle,25℃,400μmol m^(−2)s^(−1),with both shorter and longer photoperiod reducing the spore release number;4)Higher light intensity significantly increased the spore release number under 25℃,but these effects were alleviated by 30℃treatment.This study is the first attempt to elucidate the coincidence effects of photoperiod,temperature and light intensity on the reproduction of Ulva,which would help to reveal the mechanism of the rapid proliferation of green tide.
基金financially supported by the National Key R&D Program of China(Nos.2018YFD0900305,2018YFD 0901500)the China Agriculture Research System of MOF and MARA,Central Public-interest Scientific Institution Basal Research Fund CAFS(No.2020TD27)the‘Young Talent of Fishery Sciences’project from Laboratory for Marine Fisheries and Aquaculture(No.2018-MFS-T12).
文摘Saccharina japonica gametophytes can survive a long period under unfavorable environmental conditions,while they also delay in growth and/or reproduction.Although the reproduction in delayed gametophyte of S.japonica was known to be strongly influenced by light intensity,light quality,and photoperiod,no previous studies have evaluated their interactive effects on gametogenesis.To evaluate these effects,we used an orthogonal experiment to expose delayed gametophytes of S.japonica to different light intensities,light qualities,and photoperiods for 12 days.The results showed that changes in light intensity rather than light quality and photoperiod significantly affected the relative growth rates of the delayed gametophytes.Blue light had the greatest promotion on reproduction rate.The optimal light conditions in the early vegetative growth phase in gametogenesis induction for the delayed gametophytes were at 60–80μmol photons m^(−2) s^(−1) with daylength of 12 or 16 hours under white or blue light.When the delayed gametophytes were maintained in a constant light condition from delayed state to gametogenesis,the beneficial photoperiods for vegetative growth and reproductive rate were both 16L(16 hours of light):8D(8 hours of dark).However,when the delayed S.japonica gametophytes achieve the optimal growth state during the first 6 days and then they were cultured at different light conditions for the following 6 days,the reproduction rate increased as the daylength decreased and attained a peak value in group of 8L:16D photoperiod,indicating that photoperiod adjustment at the transition period is crucial in the gametogenesis induction process of delayed gametophyte of S.japonica.
文摘In this study, an effective environment for Chlorella vulgaris growth is sought after. As a substitute source of food and feed, increasing the cell density of Chlorella culture is one of the keys to ensuring sustainability. It can be showed from different studies that optimum light intensity and pH could increase cell density. In this study, the effects of light and pH on the growth rate of C. vulgaris were observed in photobioreactor. A specific wavelength (682 nm) was determined by UV-Vis Spectrophotometry to carry out the further analysis. The light intensities were set at 7409, 9261 and 11,113 lux;pH values were set at 7, 8 and 9 respectively. The experimental results depicted the light intensity of 9261 lux as the best due to the higher number of cells (48.56 × 10<sup>6</sup> cells/mL) obtained using this intensity. In terms of pH, without pH control, cell numbers were found to be highest under the light intensity of 9261 lux. When pH was controlled, it was found that under the optimum light intensity, pH control between 7.0 and 7.5 was the optimum range for the growth of C. vulgaris. Moreover, this method of study may possibly be a promising source of low cost culture for Chlorella vulgaris.
基金supported by the National Natural Science Foundation of China (31571615)the earmarked fund for China Agriculture Research System (CARS-04-PS19)。
文摘Under natural conditions, plants constantly encounter various biotic and abiotic factors, which can potentially restrict plant growth and development and even limit crop productivity. Among various abiotic factors affecting plant photosynthesis, light serves as an important factor that drives carbon metabolism in plants and supports life on earth. The two components of light(light quality and light intensity) greatly affect plant photosynthesis and other plant's morphological, physiological and biochemical parameters. The response of plants to different spectral radiations and intensities differs in various species and also depends on growing conditions. To date, much research has been conducted regarding how different spectral radiations of varying intensity can affect plant growth and development. This review is an effort to briefly summarize the available information on the effects of light components on various plant parameters such as stem and leaf morphology and anatomy, stomatal development, photosynthetic apparatus, pigment composition, reactive oxygen species(ROS) production, antioxidants, and hormone production.
文摘Central serous chorioretinopathy(CSC) is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains obscure. In this paper, we hypothesized that CSC may result from a response of choroidal vessels to an acute increase in the environmental light intensity leading to a focal leakage from the choroidal vessels.High levels of glucocorticoids, in our proposed model,may cause persistence rather than initiation of the focal leakage, probably by suppressing the synthesis of collagen and extracellular matrix components and inhibiting fibroblastic activity.
基金supported by the Natural Science Foundation of Guangdong Province,China(S2012020011043)the National High Technology Research and Development Program of China(2014AA10A605)+2 种基金the Special Fund for Agro-scientific Research in the Public Interest(201503106)Modern Agriculture Industry Technology System for Rice in Guangdong Province(2016LM1066,2017LM1066,2018LM1066)the Swiss Agency for Development and Cooperation through its funding of “Closing Rice Yield Gaps in Asia” Project(CORIGAP)
文摘Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.
基金Supported by the National Natural Science Foundation of China(No.31800457)the Natural Science Foundation of Hubei(No.2016CFB355)。
文摘Bound extracellular polymeric substances(bEPS)play an important role in the proliferation of Microcystis.However,the understanding of bEPS characterization remains limited.In this study,threedimensional fluorescence excitation-emission matrix(3D-EEM)spectroscopy and zeta potentiometer were used to characterize the loosely bound EPS(LB-EP S)and tightly bound EPS(TB-EPS)from two dominant Microcystis morphospecies from Taihu Lake(China)at different light intensities.Physiochemical analysis showed that the growth and TB-EPS or bEPS contents in Microcystis aeruginosa were higher than those in Microcystisfl os-aquae at each light intensity.The 3D-EEM contour demonstrated that the intensities of peak B(tryptophan-like substances)in the TB-EPS from M.aeruginosa were stronger than those from M.flosaquae when the light intensity was higher than 10μE/(m^(2)·s).Zeta potential analysis showed that the absolute values of the zeta potential of TB-EPS in the two species both increased with rising light intensity,except those of TB-EPS in M.aeruginosa at 105μE/(m^(2)·s).Moreover,the absolute values of the zeta potential of M.aeruginosa were higher than tho se of M.flos-aquae at each light intensity.All these re sults indicated that M.aeruginosa may more quickly proliferate than M.flos-aquae through increased negative charges,bEPS contents.growth.and tryptophan-like substance contents at certain light intensities.
文摘The active compounds in herb drugs are mainly secondary metabolites, which are greatly influenced by external conditions. Particularly, light intensity has a great influence on the photosynthesis and accumulation of secondary metabolites. In this study, the light intensity was changed, and the influence of the light intensity on leaf photosynthetic characteristics, antioxidant enzyme activity and flavone contents of Lithocarpus litseifoliusp (Hance) Chun. was discussed. The results showed that (1) L. litseifolius is a typical heliophyte. As the light intensity decreased, the contents of chlorophyll a (Chl a), chlorophyll b (Chl b) and total chlorophyll (Chl a + b) all increased. However, the Chl a/b ratio gradually decreased. The daily variation of net photosynthetic rate changed from a double-peak curve under natural light to single-peak curve under 20% full light two shading nets. (2) As the light intensity decreased, the SOD and POD activities and the soluble proteins content decreased in the leaves. In contrast, the malondialdehyde content increased. (3) The flavone content was the highest in leaves at one shading nets 60% of full light, and the lowest occurred in leaves at two shading nets 20% of full light. A comprehensive analysis indicated that an appropriate shading could induce shade tolerance in the leaves of L. litseifolius, which promoted the accumulation of flavonoids. L. litseifolius should be planted in the lower position of sunny slope or the upper position of shady slope to increase the content of flavone in the leaves.
基金This work was funded by the National Natural Science Foundation of China(Grant Nos.31971704,31770743).
文摘Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fades away in summer and winter.Light intensity is one of the most important factors affecting leaf color along the seasons.In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves,Ananas comosus var.bracteatus was grown under full sunlight,50%shade and 75%shade for 75 days to evaluate the concentration of pigments,the color parameters(values L^(*),a^(*),b^(*))and the morpho-anatomical variations of chimeric leaves.The results showed that a high irradiance was beneficial to keep the chimeric leaves red.However,prolonged exposure to high irradiance caused a damage,some of the leaves wrinkled and even burned.Shading instead decreased the concentration of anthocyanin and increased the concentration of chlorophyll,especially in the white marginal part of the leaves.Numerous chloroplasts were observed in the mesophyll cells of the white marginal part of the chimeric leaves under shading for 75 days.The increase in chlorophyll concentration resulted in a better growth of plants.In order to balance the growth and coloration of the leaves,approximately 50%shade is suggested to be the optimum light irradiance condition for Ananas comosus var.bracteatus in summer.
基金China Agriculture Research System(Nos.2019-3-5-1-02,2019-3-4-4,F18R06001-1)China Agriculture Research System of MOF and MARA(No.CARS-30-Z-02)Hebei Province Key Research and Development Project(No.17226341).
文摘Light is crucial to the uniform production of high-quality fruit since it is the driving force for leaf photosynthesis and hence overall plant nutrition.The objective of this study was to evaluate the relationship between the distribution of relative light intensity in the peach tree(Prunus persica)canopy and the yield and texture of peach fruits.The canopy of 7-year-old‘Qiuyan’was divided into cubical volumes and the relative light intensity distribution was measured for each cube,along with yield distribution and fruit textural properties at different growing times.The relative light intensity decreased gradually from outside to inside the canopy and from top to bottom.The yield distribution and the relative light intensity were clearly correlated.The percentage of the canopy receiving<30%relative light intensity was 49.07%in May,56.02%in June,and 58.95%in July,whereas the percentage receiving>80%relative light intensity was 32.72%,17.28%,and 10.96%,respectively.Consistent with this,peaches were found in the upper and middle portions of the canopy,within 1.5-3.0 m of the top.The regression equation showed that fruit texture index correlated significantly with relative light intensity.Relative light intensity is the main factor affecting peach yield and texture and must be above 41.83%for good peach quality.Orchardists should carefully plan summer pruning strategies to adjust the number and spatial distribution of branches accordingly.
基金This study was funded by the China-ASEAN Maritime Cooperation Fund(China-ASEAN Center for Joint Research and Promotion of Marine Aquaculture Technology,No:DF)and China Postdoctoral Science Foundation Funded Project(Project No:2018M641984)。
文摘Light is an essential natural factor of fish growth and development.A light regime in indoor aquaculture is critical for sustainable fish production.This research aimed to investigate the effect of light intensity and photoperiod on the growth performance and stress response of Nile tilapia(Oreochromis niloticus),which is an important commercial species,in a recirculating aquaculture system(RAS).Fingerlings with an average weight of 5±0.9 g were randomly assigned to nine 1.5 m3 tanks with 145 fish in each tank,cultured under different light intensities(1000,2000,and 3000 lx)and photoperiods(12L:12D,18L:6D,24L:0D),and fed to apparent satiation twice a day for 160 days.The growth of tilapia subjected to 2000 lx was significantly better than that exposed to 1000 and 3000 lx(P<0.05)as indicated by the final weight(351.17±10.59 g),growth efficiency(0.77±0.26),specific growth rate(2.65%±0.21%per day)and feed conversion coefficient ratio(1.30±0.36).No significant difference was observed in the growth rate among different photoperiod groups(P>0.05).Light intensity and photoperiod manipulation did not cause a significant chronic stress response in tilapia.This study demonstrated that light intensity,especially at 2000 lx,and photoperiod manipulation could stimulate the growth of tilapia in the RAS and significantly affect economic profitability.This study also served as an important reference for tilapia indoor aquaculture.
基金study was provided by the National Key Research and Development Project:Precise Pond Culture Technology and Aquatic Product Quality Improvement Model"(2019YFD0900303)the Shanghai Agriculture Applied Technology Development Program,China(Grant No.X20210301)+1 种基金the China-ASEAN Maritime Cooperation Fund(China-ASEAN Center for Joint Research and Promotion of Marine Aquaculture Technology)China Postdoctoral Science Foundation Funded Project(Project No.:2018M641984)。
文摘Murray cod(Maccullochella peelii)is a freshwater percichthyid fish that has a high market value and potential for culture in recirculating aquaculture systems(RASs).Illumination is an important environmental factor that affects the growth and physiological condition of fishes.A comprehensive understanding of the relationship between the light factors and the growth,nutrient composition and stress response of juvenile Murray cod in RAS is important to achieve satisfactory theoretical and practical aquaculture performance.Juvenile Murray cod were randomly assigned to nine RAS tanks with a volume of 1.5 m^(3),with each tank containing 120 fishes(3.5±0.5g).The fish were cultured for 120 days under different light intensities(1200,2400,and 3600 lx)and photoperiods(12L:12D,18L:6D and 24L:0D).The results showed that the final weight and feed conversion ratio of Murray cod under a light intensity of 1200 lx were significantly better than those under 3600 lx(P<0.05).The serum total protein and globulin were higher at the light intensity of 1200lx,compared to other light intensities.The blood urea nitrogen level improved with increasing light intensity at LD12:12 and LD18:6 of photoperiod,as well as the superoxide dismutase level improved significantly from LD18:6 to LD24:0 of photoperiod.The findings indicated that the optimal light condition to enhance growth at juvenile stage is 1200 lx of the light intensity and LD18:6 of the photoperiod.
基金Supported by the National"the 13th Five-Year"Key R&D Program(2016YFD0701905)。
文摘Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydroponically grown arugula was investigated by using light-emitting diodes(LEDs)to light the hydroponically grown arugula for a reference for industrialized arugula production.The dynamic demands of arugula for LI in the seedling stage,initial growth stage and vigorous growth stage were tested under two light quality conditions including a red/blue light ratio of 7:1 and a light/dark photoperiod of 12 h/12 h.Then,the curves of variable LI-induced changes in the growth indices of arugula in different development periods were drawn.Next,the influence of variable LI on the growth characteristics and nutritional quality of arugula was investigated by measuring the dry/fresh weight ratio,chlorophyll content,vitamin C content and soluble protein content.Variable LI significantly increased the height,stem diameter,leaf width,dry/fresh weight ratio,chlorophyll content and soluble protein content of arugula plant.Plant height,stem diameter,dry/fresh weight ratio,chlorophyll content and soluble protein content were the highest in the group exposed to LI of 200,300 and 300μmol•m^(-2)•s^(-1)during the seedling stage,initial growth stage and vigorous growth stage,respectively.The greatest leaf width was achieved at LI of 100,250 and 350μmol•m^(-2)•s^(-1),respectively.High intensity LI markedly repressed the synthesis of vitamin C.
基金supported by projects funded by grants from the Natural Science Foundation of Jiangsu Province in China(BK20221515)the National Natural Science Foundation of China(32172266)the Changzhou Science and Technology Support Program(CE20222002)。
文摘The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.
基金The authors acknowledge the support of the H2020 pro-gram for Solar-ERANET funding of the BOBTANDEM(2019-2022).
文摘The current climate and energy crisis urgently needs solar cells with efficiencies above the 29% single junction efficiency bottleneck.Silicon/perovskite tandem solar cells are a solution,which is attracting much attention.While silicon/perovskite tandem cells in 2-terminal and 4-terminal configurations are well documented,the three-terminal concept is still in its infancy.It has significant advantages under low light intensities as opposed to concentrated sunlight,which is the critical factor in designing tandem solar cells for low-cost terrestrial applications.This study pre-sents novel studies of the sub-cell performance of the first three-terminal perovskite/silicon selective band offset barrier tandem solar cells fabricated in an ongoing research project.This study focuses on short circuit current and operating voltages of the subcells under light intensities of one sun and below.Lifetime studies show that the perovskite bulk carrier lifetime is insensitive to illumination,while the silicon cell's lifetime decreases with decreasing light intensity.The combination of perovskite and silicon in the 3T perovskite-silicon tandem therefore reduces the sensitivity of V_(OC) to light intensity and maintains a relatively higher V_(OC) down to low light intensities,whereas silicon single-junction cells show a marked decrease.This technological advantage is proposed as a novel advantage of three-terminal perovkite/silicon solar cells for low light intensities of one sun or less.
基金supported by China Agriculture Research System of MOF and MARA(CARS-21).
文摘Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.
基金partly supported by Grant-in-Aid for scientific research(No.17658070,22580173)from the Ministry of Education,Science and Culture,Japan“Evaluation of genetic resources for strengthening productivity and adaptability of tropical forests”from the Japan International Research Centre for Agricultural Sciences。
文摘Although the distributions of foliage and light play major roles in various forest functions,accurate,nondestructive measurement of these distributions is difficult due to the complexity of the canopy structure.To evaluate the foliage and light distributions directly and nondestructively in a mature oak stand,we used the cube method by dividing the forest canopy into small cubes(50 cm per side)and directly measured leaf area density(LAD,the total one-sided leaf area per unit volume,i.e.,cube)and relative irradiance(RI)within each cube.The distribution of LAD and of RI was highly heterogeneous,even at the same canopy height.This heterogeneity reflected the presence of foliage clusters associated with multiple forking branches.The relationship between cumulative LAD at the canopy surface and average RI followed the Beer-Lambert law.The mean light extinction coefficient(K)was 0.32.However,K was overestimated by more than double(0.80)when calculated based on the classical method using RI at the forest floor.This overestimation was caused by the lower RI due to light absorption by nonleaf plant parts below the canopy.Our findings on the complex foliage and light distributions in canopy layers should help improve the accuracy of RI and K measurements and thus more accurate predictions of environmental responses and forest functions.
文摘Objective To evaluate the effect of intense pulsed light(IPL)in the treatment of chronic hordeolum.Methods Patients with chronic hordeolum who underwent IPL treatment were enrolled in this study.According to the severity of hordeolum,the patients were treated with IPL 3 to 5 times.Patients’satisfaction and visual analog scale scores for ocular discomfort symptoms before and after treatment were collected.The number,congestion,long diameter,short diameter and area of nodules were also recorded and measured.Finally,eyelid margin signs,meibum quality,meibomian gland expressibility,meibomian gland dropout,tear meniscus height,and corneal fluorescein staining were scored.Results 20 patients were enrolled in this study.The eyelid margins were congestive and swollen,with blunt rounding or irregularity.The meibum was cloudy or toothpaste-like.The meibomian gland expressibility,meibomian gland dropout and tear meniscus height were reduced.The cornea showed scattered fluorescein staining.After treatment,score of visual analog scale,congestion and size of nodules were significantly reduced.Eyelid margin signs,meibum quality,meibomian gland expressibility,tear meniscus height and corneal fluorescein staining scores were improved.Meibomian gland dropout had no significant change.No side effects occurred during treatment.Conclusions IPL is beneficial for the treatment of chronic hordeolum.
基金This research was supported by the National Natural Sci-ence Foundation of China(No.31872555)the China Agri-culture Research System(No.CARS-50)the Key Pro-gram of Science and Technology Innovation Ningbo(No.2019B10009).
文摘Three Gracilariopsis lemaneiformis strains,including wild type and high-temperature-resistant cultivars 981 and 2007,were studied with the changes in their photosynthetic pigment contents and related gene transcription levels under different light intensities(10,60,100,and 200μmolm^(−2)s^(−1)).The three G.lemaneiformis strains had the following photosynthetic pigments with high-to-low contents:phycoerythrin(PE),phycocyanin(PC),allophycocyanin(APC),and chlorophyll a(Chl a).Among the three strains,cultivar 981 had the highest PE content,followed by cultivar 2007.The PC and APC contents were similar among the three strains,but they were higher in cultivars 981 and 2007 than in the wild type.The Chl a contents in the three G.lemaneiformis strains were equal.A low light intensity(10μmolm^(−2)s^(−1))promoted photosynthetic pigment accumulation in G.lemaneiformis and improved the relative PE gene transcription(peA and peB)in a short period(≤6 d).A high light intensity decreased the PE content.PebA and PebB,which catalyzed phycoerythrobilin synthesis,showed no compensatory upregulation at a low light intensity among the strains except for the wild type.At a high light intensity,transcription levels of pebA and pebB in the three strains were upregulated.This study provided an experimental basis for elucidating the photosynthesis of G.lemaneiformis.As key genes of algal growth,photo-synthesis-related genes served as useful gene markers for screening elite varieties with good traits in breeding.Cultivar 2007 was superior to cultivar 981 in terms of maintaining high pigment levels in a wide range of light intensities,which is the most suitable for aquaculture.
文摘Harmful algae bloom constitutes a major problem facing water bodies particularly fresh and marine water system. <i>Microcystis aeruginosa</i> represents a major causative organism found in the water. Light plays a major role in the growth and variation of <i>M. aeruginosa</i> in water. What is still inadequate, is the data on the effects of different light conditions on the growth of <i>Microcystis aeruginosa</i>. In this study, two strains of <i>Microcystis aeruginosa</i> PCC 7806 (toxic strain) and PCC 7005 (non-toxic strain) were exposed to varying light intensities at 0, 30, 60, 120, 300, 350 μMol photon m<sup>-2</sup>·S<sup>-1</sup> to measure their growth and pigmentation. This study observed similarities in growth and pigmentation between toxic strain (PCC 7806) and non-toxic (PCC 7005) strain. Also, at short exposure to light, Toxic strain was seen to be better tolerant of light than non-toxic strain, with non-toxic strain showing better ability to recover from light stress after twenty days of culture. These findings suggest that Toxic strain (PCC 7806) and Non-toxic strain (PCC 7005) of <i>M. aeruginosa</i> respond similarly with respect to growth but differ in their light retention capacity over time and this could be useful in predicting the possible conditions of algal bloom.
