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EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1,TRF2,and RAD50 被引量:2
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作者 Kai-Qin Li Gao-Jing Liu +10 位作者 Xiu-Yun Liu Qiong-Fang Chen Xiao-Yan Huang Qiu Tu Jiao Zhang Qing Chang Yun-Hua Xie Rong Hua Dong-Ming Xu Zhen Liu Bo Zhao 《Zoological Research》 SCIE CAS CSCD 2023年第3期636-649,共14页
Telomeres are nucleoprotein structures located at the end of each chromosome,which function in terminal protection and genomic stability.Telomeric damage is closely related to replicative senescence in vitro and physi... Telomeres are nucleoprotein structures located at the end of each chromosome,which function in terminal protection and genomic stability.Telomeric damage is closely related to replicative senescence in vitro and physical aging in vivo.As relatively long-lived mammals based on body size,bats display unique telomeric patterns,including the upregulation of genes involved in alternative lengthening of telomeres(ALT),DNA repair,and DNA replication.At present,however,the relevant molecular mechanisms remain unclear.In this study,we performed cross-species comparison and identified EPAS1,a well-defined oxygen response gene,as a key telomeric protector in bat fibroblasts.Bat fibroblasts showed high expression of EPAS1,which enhanced the transcription of shelterin components TRF1 and TRF2,as well as DNA repair factor RAD50,conferring bat fibroblasts with resistance to senescence during long-term consecutive expansion.Based on a human single-cell transcriptome atlas,we found that EPAS1 was predominantly expressed in the human pulmonary endothelial cell subpopulation.Using in vitro-cultured human pulmonary endothelial cells,we confirmed the functional and mechanistic conservation of EPAS1 in telomeric protection between bats and humans.In addition,the EPAS1 agonist M1001 was shown to be a protective compound against bleomycin-induced pulmonary telomeric damage and senescence.In conclusion,we identified a potential mechanism for regulating telomere stability in human pulmonary diseases associated with aging,drawing insights from the longevity of bats. 展开更多
关键词 Bat TELOmERE SENESCENCE epaS1 m1001 Pulmonary endothelial cell
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Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak(Bos grunniens) 被引量:8
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作者 WU Xiao-yun DING Xue-zhi +4 位作者 CHU Min GUO Xian BAO Peng-jia LIANG Chun-nian YAN Ping 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第4期741-748,共8页
Endothelial PAS domain protein 1 gene (EPAS1) is a key transcription factor that activates the expression of oxygen-regu- lated genes. In this study, in order to better understand the effects of EPAS1 gene on hemato... Endothelial PAS domain protein 1 gene (EPAS1) is a key transcription factor that activates the expression of oxygen-regu- lated genes. In this study, in order to better understand the effects of EPAS1 gene on hematologic parameters in yak, we firstly quantified the tissue expression patterns for EPASl mRNA of yak, identified polymorphism in this gene and evaluated its association with hematologic parameters. Expression of EPAS1 mRNA was detected in all eight tissues (heart, liver, lung, spleen, pancreas, kidney, muscles and ovary). The expressions of EPAS1 in lung and pancreas were extremely higher than other tissues examined. Three novel single nucleotide polymorphisms (SNPs) (g.83052 C〉T, g.83065 G〉A and g.83067 C〉A) within the EPAS1 were identified and genotyped in Pali (PL), Gannan (GN) and Tianzhu White (TZW) yak breeds. Significant higher frequencies of the AA and GA genotypes and A allele of the g.83065 G〉A were observed in the PL and GN breeds than that in the TZW breed (P〈0.01). Association analysis of the PL breed indicated that the g.83065 G〉A polymorphism was significantly associated with hemoglobin (HGB) concentration in yaks (P〈0.05). Individuals with genotype AA had significantly higher HGB concentration (P〈0.05) than those with genotype GA and GG. All these results will help our further understanding of biological functional of yak EPAS1 gene in responding to hypoxia and also indicate EPAS1 might contribute to the hypoxia adaptation of the yak. 展开更多
关键词 epaS1 gene m RNA expression POLYmORPHISm hypoxia adaptation YAK
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Escherichia coli Producing CTX-M and DHA-1 Beta-lactamases in Farm Animals in Chongqing City of China
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作者 WU Jun-wei ZHANG Zhen-zhen WANG Kai-yu 《畜牧兽医学报》 CAS CSCD 北大核心 2010年第S1期97-101,共5页
Aim of this study was to investigate the genotypic distribution of extended-spectrum β-lactamases (ESBLs) and AmpC enzymes among Escherichia coli isolated from farm animals in Chongqing City. 326 E.coli isolates were... Aim of this study was to investigate the genotypic distribution of extended-spectrum β-lactamases (ESBLs) and AmpC enzymes among Escherichia coli isolated from farm animals in Chongqing City. 326 E.coli isolates were obtained from piglets,cattle and chickens.In these isolates,19 ESBLs and 6 AmpC-producing E.coli isolates were detected by preliminary screening and phenotypic identification, and polymerase chain reaction and sequence analysis methods were used to detect ESBLs and AmpCβ-lactamases genes.The results showed that the frequencies of TEM,CTX-M,DHA-1 and CMY-2 genes were 2.15%,5.21%,0.61%and 0.61%,respectively,in E.coli of animal origin in the Chongqing area,and 5 E.coli isolates were found to carry two kinds of genotype.The DHA-1 gene was detected for the first time in a veterinary clinic in China;its GenBank accession number is FJ386455.This study indicated that TEM and CTX-M genes were the predominant genotypes in Chongqing,and the majority of ESBLs and AmpC-producing E.coli isolates were multi-resistant to antibiotics,so they deserve particular attention in veterinary clinics. 展开更多
关键词 Escherichia coli farm animals CTX-m dha-1 China
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真鲷仔稚鱼微粒饲料中DHA与EPA最佳比例的研究 被引量:19
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作者 刘镜恪 陈晓琳 +1 位作者 周利 雷霁霖 《海洋科学》 CAS CSCD 北大核心 2004年第2期18-20,共3页
制备4种n-3HUFA含量相同 ,DHA和EPA含量比(m(DHA)∶m(EPA))分别为1.70∶1 ,2.00∶1,2.30∶1和2.60∶1的微粒饲料 ,探讨DHA与EPA的配比对真鲷 (Pagrusmajor)仔稚鱼生长、存活和体内相关成分的影响。30d的养殖试验结果表明 ,m(DHA)∶m(EPA... 制备4种n-3HUFA含量相同 ,DHA和EPA含量比(m(DHA)∶m(EPA))分别为1.70∶1 ,2.00∶1,2.30∶1和2.60∶1的微粒饲料 ,探讨DHA与EPA的配比对真鲷 (Pagrusmajor)仔稚鱼生长、存活和体内相关成分的影响。30d的养殖试验结果表明 ,m(DHA)∶m(EPA)≥2.30∶1的2种微粒饲料与m(DHA)∶m(EPA)≤2.00∶1的2种微粒饲料相比 ,真鲷仔稚鱼的生长速度和成活率 ,前者明显优于后者。该比例即m(DHA)∶m(EPA)≥2.30∶1应是真鲷微粒饲料中DHA与EPA的最佳配比。养殖试验结束后 ,对仔稚鱼体内相关成分的分析结果表明 ,仔稚鱼体内DHA的含量随微粒饲料中DHA含量的增加而增大。 展开更多
关键词 真鲷 微粒饲料 仔稚鱼 dha epa 最佳比例
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全国10所教学医院产ESBLs和质粒AmpC酶大肠埃希菌及肺炎克雷伯菌的研究 被引量:31
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作者 孙宏莉 宁永忠 +2 位作者 廖康 王辉 朱任媛 《中国感染与化疗杂志》 CAS 2007年第5期323-329,共7页
目的研究全国10所教学医院临床分离的大肠埃希菌和肺炎克雷伯菌中同时产ESBLs及质粒型AmpC酶菌株的比率及其基因型特点,比较不同地区间的差别。方法收集来自中国不同地区的10所教学医院2003年度产ESBLs的大肠埃希菌90株和肺炎克雷伯菌61... 目的研究全国10所教学医院临床分离的大肠埃希菌和肺炎克雷伯菌中同时产ESBLs及质粒型AmpC酶菌株的比率及其基因型特点,比较不同地区间的差别。方法收集来自中国不同地区的10所教学医院2003年度产ESBLs的大肠埃希菌90株和肺炎克雷伯菌61株,用等电聚焦电泳测定β内酰胺酶的等电点;接合试验证实酶基因有无可转移性;采用多重聚合酶链反应(MPCR)及序列分析确定质粒AmpC酶的基因型。结果武汉、南京、济南等地产ESBLs大肠埃希菌及肺炎克雷伯菌所占比率相近,均在50%左右;北京产ESBLs大肠埃希菌所占比率低于肺炎克雷伯菌;其他各城市产ESBLs大肠埃希菌所占比率均略高于肺炎克雷伯菌。在产ESBLs菌株中24.4%大肠埃希菌和19.4%肺炎克雷伯菌对头孢西丁耐药。除沈阳无对头孢西丁耐药菌株外,其他医院均存在对头孢西丁耐药株。产ESBLs菌株基因型主要为CTX—M型,其中以CTX-M-9组最为常见,其次为CTX-M-1组。在同时产ESBLs和AmpC的菌株中,肺炎克雷伯菌多于大肠埃希菌,且均为CTX-M/ DHA-1型(多为CTX-M-9/DHA-1型)。3株同时产ESBLs和AmpC肺炎克雷伯菌的接合子PCR结果与其供体菌完全相同。结论参与本研究的各所教学医院产ESBLs菌株基因型主要为CTX-M-9组,在同时产ESBLs和AmpC酶的菌株中,肺炎克雷伯菌多于大肠埃希菌,多为CTX-M-9/DHA-1型,该酶可通过质粒传播。 展开更多
关键词 超广谱Β内酰胺酶 质粒型AmPC酶 CTX-m-9组酶 dha-1型酶
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DHA诱导3T3-L1前脂肪细胞G2/M期阻滞及ERK1/2通路的作用 被引量:1
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作者 徐琛玮 沈颖卓 +2 位作者 龚清海 陈士勇 张晓宏 《营养学报》 CAS CSCD 北大核心 2011年第4期354-357,共4页
目的探讨ERK1/2丝裂素活化蛋白激酶信号转导途径在DHA抑制3T3-L1前脂肪细胞增殖中的作用。方法四唑盐比色法(MTT法)检测DHA处理24h对体外培养的3T3-L1前脂肪细胞活力的影响,流式细胞术检测细胞周期,蛋白免疫印迹法检测ERK1/2、p-ERK1/2... 目的探讨ERK1/2丝裂素活化蛋白激酶信号转导途径在DHA抑制3T3-L1前脂肪细胞增殖中的作用。方法四唑盐比色法(MTT法)检测DHA处理24h对体外培养的3T3-L1前脂肪细胞活力的影响,流式细胞术检测细胞周期,蛋白免疫印迹法检测ERK1/2、p-ERK1/2、p21水平。结果 MTT结果显示DHA干预24h可剂量依赖性降低3T3-L1前脂肪细胞的活力,抑制增殖,IC50为100μmol/L;流式细胞术结果表明,DHA可将3T3-L1前脂肪细胞阻滞在G2/M期;蛋白免疫印迹发现,DHA可明显升高3T3-L1前脂肪细胞ERK1/2磷酸化水平、增强p21蛋白表达。结论 DHA可能通过活化ERK1/2通路诱导G2/M期阻滞,进而抑制3T3-L1前脂肪细胞增殖。 展开更多
关键词 dha 3T3-L1前脂肪细胞 G2/m期阻滞 ERK1/2通路
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