Two simple, accurate, precise and economic spectrophotometric methods have been developed for simultaneous determination of Atorvastatin calcium (ATR) and Ezetimibe (EZ) in their bulk powder and pharmaceutical dosage ...Two simple, accurate, precise and economic spectrophotometric methods have been developed for simultaneous determination of Atorvastatin calcium (ATR) and Ezetimibe (EZ) in their bulk powder and pharmaceutical dosage form. Method (I) is based on dual wavelength analysis while method (II) is the mean centering of ratio spectra spectrophotometric (MCR) method. In method (I), two wavelengths were selected for each drug in such a way that the difference in absorbance was zero for the second drug. At wavelengths 226.6 and 244 nm EZ had equal absorbance values; therefore, these two wavelengths have been used to determine ATR; on a similar basis 228.6 and 262.8 nm were selected to determine EZ in their binary mixtures. In method II, the absorption spectra of both ATR and EZ with different concentrations were recorded over the range 200-350, divided by the spectrum of suitable divisor of both ATR and EZ and then the obtained ratio spectra were mean centered. The concentrations of active components were then determined from the calibration graphs obtained by measuring the amplitudes at 215-260 nm (peak to peak) for both ATR and EZ. Accuracy and precision of the developed methods have been tested; in addition recovery studies have been carried out in order to confirm their accuracy. On the other hand, selectivities of the methods were tested by application for determination of different synthetic mixtures containing different ratios of the studied drugs. The developed methods have been successfully used for determination of ATR and EZ in their combined dosage form and statistical comparison of the developed methods with the reported spectrophotometric one using F and Student's t-tests showed no significant difference regarding both accuracy and precision.展开更多
Explicitly identifying the spatial distribution of ecological transition zones(ETZs)and simulating their response to climate scenarios is of significance in understanding the response and feedback of ecosystems to glo...Explicitly identifying the spatial distribution of ecological transition zones(ETZs)and simulating their response to climate scenarios is of significance in understanding the response and feedback of ecosystems to global climate change.In this study,a quantitative spatial identification method was developed to assess ETZ distribution in terms of the improved Holdridge life zone(iHLZ)model.Based on climate observations collected from 782 weather stations in China in the T0(1981–2010)period,and the Intergovernmental Panel on Climate Change Coupled Model Intercomparison Project(IPCC CMIP5)RCP2.6,RCP4.5,and RCP8.5 climate scenario data in the T1(2011–2040),T2(2041–2070),and T3(2071–2100)periods,the spatial distribution of ETZs and their response to climate scenarios in China were simulated in the four periods of T0,T1,T2,and T3.Additionally,a spatial shift of mean center model was developed to quantitatively calculate the shift direction and distance of each ETZ type during the periods from T0 to T3.The simulated results revealed 41 ETZ types in China,accounting for 18%of the whole land area.Cold temperate grassland/humid forest and warm temperate arid forest(564,238.5 km~2),cold temperate humid forest and warm temperate arid/humid forest(566,549.75 km~2),and north humid/humid forest and cold temperate humid forest(525,750.25 km~2)were the main ETZ types,accounting for 35%of the total ETZ area in China.Between 2010 and 2100,the area of cold temperate desert shrub and warm temperate desert shrub/thorn steppe ETZs were projected to increase at a rate of 4%per decade,which represented an increase of 3604.2,10063.1,and 17,242 km~2 per decade under the RCP2.6,RCP4.5,and RCP8.5 scenarios,respectively.The cold ETZ was projected to transform to the warm humid ETZ in the future.The average shift distance of the mean center in the north wet forest and cold temperate desert shrub/thorn grassland ETZs was generally larger than that of other ETZs,with the mean center moving to the northeast and the shift distance being more than 150 km during the periods from T0 to T3.In addition,with a gradual increase of temperature and precipitation,the ETZs in northern China displayed a shifting northward trend,while the area of ETZs in southern China decreased gradually,and their mean center moved to high-altitude areas.The effects of climate change on ETZs presented an increasing trend in China,especially in the Qinghai-Tibet Plateau.展开更多
文摘Two simple, accurate, precise and economic spectrophotometric methods have been developed for simultaneous determination of Atorvastatin calcium (ATR) and Ezetimibe (EZ) in their bulk powder and pharmaceutical dosage form. Method (I) is based on dual wavelength analysis while method (II) is the mean centering of ratio spectra spectrophotometric (MCR) method. In method (I), two wavelengths were selected for each drug in such a way that the difference in absorbance was zero for the second drug. At wavelengths 226.6 and 244 nm EZ had equal absorbance values; therefore, these two wavelengths have been used to determine ATR; on a similar basis 228.6 and 262.8 nm were selected to determine EZ in their binary mixtures. In method II, the absorption spectra of both ATR and EZ with different concentrations were recorded over the range 200-350, divided by the spectrum of suitable divisor of both ATR and EZ and then the obtained ratio spectra were mean centered. The concentrations of active components were then determined from the calibration graphs obtained by measuring the amplitudes at 215-260 nm (peak to peak) for both ATR and EZ. Accuracy and precision of the developed methods have been tested; in addition recovery studies have been carried out in order to confirm their accuracy. On the other hand, selectivities of the methods were tested by application for determination of different synthetic mixtures containing different ratios of the studied drugs. The developed methods have been successfully used for determination of ATR and EZ in their combined dosage form and statistical comparison of the developed methods with the reported spectrophotometric one using F and Student's t-tests showed no significant difference regarding both accuracy and precision.
基金National Key R&D Program of China,No.2018YFC0507202,No.2017YFA0603702National Natural Science Foundation of China,No.41971358,No.41930647+1 种基金Strategic Priority Research Program(A)of the Chinese Academy of Sciences,No.XDA20030203Innovation Research Project of State Key Laboratory of Resources and Environment Information System,CAS。
文摘Explicitly identifying the spatial distribution of ecological transition zones(ETZs)and simulating their response to climate scenarios is of significance in understanding the response and feedback of ecosystems to global climate change.In this study,a quantitative spatial identification method was developed to assess ETZ distribution in terms of the improved Holdridge life zone(iHLZ)model.Based on climate observations collected from 782 weather stations in China in the T0(1981–2010)period,and the Intergovernmental Panel on Climate Change Coupled Model Intercomparison Project(IPCC CMIP5)RCP2.6,RCP4.5,and RCP8.5 climate scenario data in the T1(2011–2040),T2(2041–2070),and T3(2071–2100)periods,the spatial distribution of ETZs and their response to climate scenarios in China were simulated in the four periods of T0,T1,T2,and T3.Additionally,a spatial shift of mean center model was developed to quantitatively calculate the shift direction and distance of each ETZ type during the periods from T0 to T3.The simulated results revealed 41 ETZ types in China,accounting for 18%of the whole land area.Cold temperate grassland/humid forest and warm temperate arid forest(564,238.5 km~2),cold temperate humid forest and warm temperate arid/humid forest(566,549.75 km~2),and north humid/humid forest and cold temperate humid forest(525,750.25 km~2)were the main ETZ types,accounting for 35%of the total ETZ area in China.Between 2010 and 2100,the area of cold temperate desert shrub and warm temperate desert shrub/thorn steppe ETZs were projected to increase at a rate of 4%per decade,which represented an increase of 3604.2,10063.1,and 17,242 km~2 per decade under the RCP2.6,RCP4.5,and RCP8.5 scenarios,respectively.The cold ETZ was projected to transform to the warm humid ETZ in the future.The average shift distance of the mean center in the north wet forest and cold temperate desert shrub/thorn grassland ETZs was generally larger than that of other ETZs,with the mean center moving to the northeast and the shift distance being more than 150 km during the periods from T0 to T3.In addition,with a gradual increase of temperature and precipitation,the ETZs in northern China displayed a shifting northward trend,while the area of ETZs in southern China decreased gradually,and their mean center moved to high-altitude areas.The effects of climate change on ETZs presented an increasing trend in China,especially in the Qinghai-Tibet Plateau.
