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Relationship between Leaf Micro- and Macro-Nutrients in Top Canopy Trees in a Mixed Forest in the Upper Rio Negro in the Amazon Region
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作者 M. A. Sobrado 《American Journal of Plant Sciences》 2014年第10期1423-1431,共9页
The mixed forests of the upper Rio Negro at the northern of the Amazon basin grow in oxisol soils that are extremely infertile. These areas exhibit deficiencies in several macro-nutrients, and may also be characterize... The mixed forests of the upper Rio Negro at the northern of the Amazon basin grow in oxisol soils that are extremely infertile. These areas exhibit deficiencies in several macro-nutrients, and may also be characterized by the shortage or toxic excess of some micronutrients. The overall goal of this research is to collect more comprehensive information regarding the micronutrient composition of the upper Rio Negro forests as well as discern the relationship between leaf micro- and macro-nutrients that may contribute to the homeostasis and balance of the ionome. Firstly, the nutrient composition within the oxisol soil and leaf tissues of two top canopy tree species from the mixed forests was determined. We then analyzed the relationship between leaf micronutrient composition with N and P levels of the two species and that of species inhabiting the Amazon caatinga. Extractable soil Zn, B, Mn and Cu were very low in the mixed forest. In contrast, Fe and Al levels were potentially toxic. The analysis of leaf N/P ratios revealed for the first time the co-limitation of N and P in the mixed forest. This contrasts with species from the adjacent Amazon caatinga toposequence that are characterized by strong N limitation. All micronutrients within leaves of species inhabiting the mixed forest were also found to have low concentrations. Moreover, Fe and Al were detected at concentrations well below those reported for accumulator species. This suggested that leaf ion homeostasis was maintained under potentially toxic soil Fe and Al conditions. Leaf micronutrient (Fe, Zn and B) contents mirrored that of leaf N and P contents, and comparable Fe/N, Fe/P, Zn/N, Zn/P, B/N as well as B/P ratios were found across species and forest types. Therefore, forest species exhibited the capability to maintain leaf nutrient balances under soil conditions with deficient or toxic levels of micronutrients. 展开更多
关键词 AMAZON Forests AMAZON Caatinga LEAF Ionome Homeostasis LEAF N-P and micronutrientS micro-nutrient Deficiency micronutrientS Toxicity Mixed Forest N-P Co-Limitation Plant nutrient Balance Oxisol PODZOL
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5-羟色胺转运蛋白显像剂^(11)C-DASB的自动化合成及Micro PET/CT显像
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作者 张晓军 刘健 +2 位作者 李云钢 田嘉禾 张锦明 《同位素》 CAS 2015年第1期1-6,共6页
目的:自动化合成5-羟色胺转运蛋白显像剂11 C-DASB并进行大鼠Micro PET/CT显像;方法:通过改变甲基化试剂、溶解前体溶剂及反应条件,得到优化的标记条件作为碳-11多功能合成模块的输入参数,进行自动化合成11 C-DASB,大鼠静脉注射11 C-DAS... 目的:自动化合成5-羟色胺转运蛋白显像剂11 C-DASB并进行大鼠Micro PET/CT显像;方法:通过改变甲基化试剂、溶解前体溶剂及反应条件,得到优化的标记条件作为碳-11多功能合成模块的输入参数,进行自动化合成11 C-DASB,大鼠静脉注射11 C-DASB 45 min后进行显像;结果:采用11 C-CH3-Triflate作为甲基化试剂,通入新配制的含1mg去甲基DASB前体的500μL DMSO溶液内,80℃下加热2min,标准率为63.7%,大鼠显像表明,11 C-DASB特异性的浓聚于SERT富集区域;结论:经优化,11 C-DASB自动化合成可得到较高产率,大鼠显像表明,其特异性浓聚于SERT富集区域,有望作为5-羟色胺转运蛋白显像剂。 展开更多
关键词 ^11C-DASB 5-羟色胺转运蛋白 micro PET/CT
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^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠乳腺癌早期放疗疗效实验研究 被引量:3
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作者 苏晓雨 徐慧琴 +3 位作者 汪会 余文静 张丹 谯凤 《安徽医科大学学报》 CAS 北大核心 2019年第2期203-206,共4页
目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只... 目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只裸鼠肿瘤SUVmax值。完成显像后,常规HE染色观察每组肿瘤组织形态学特征,免疫组化方法测定每组肿瘤细胞乏氧诱导因子-1α(HIF-1α)的表达情况。结果放疗前,对照组与放疗组SUVmax值差异无统计学意义(t=0. 375,P> 0. 05)。放疗组放疗后48 h裸鼠肿瘤组织SUVmax值较放疗前(t=9. 958,P <0. 05)、放疗后24 h(t=16. 506,P <0. 05)明显降低,差异有统计学意义(F=58. 860,P <0. 05)。放疗后24 h SUVmax值也低于放疗前24 h(t=5. 405,P <0. 05),差异有统计学意义。HE染色结果显示放疗组肿瘤细胞坏死较对照组更加明显。免疫组化结果显示放疗组放疗后HIF-1α表达阳性率明显低于放疗前(t=14. 802,P <0. 05),差异具有统计学意义。相关性分析结果显示肿瘤SUVmax与HIF-1α的表达呈明显正相关性(r=0. 865,P <0. 05)。结论^(18)F-氟赤硝基咪唑micro PET/CT可以监测肿瘤内部的乏氧状态,并且可以评价裸鼠MDA-MB231乳腺癌的早期放疗疗效。 展开更多
关键词 18F-氟赤硝基咪唑 micro PET/CT 放疗疗效 乏氧诱导因子-
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Microstructure Study of Normal Lunates with Micro-computed Tomography
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作者 肖滋润 熊革 +2 位作者 shi-gong guo 杜传超 张友乐 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第3期384-389,共6页
In order to study the microstructure characteristics of normal lunate bones, eight fresh cadaver normal lunates were scanned with micro-computed tomography. High-resolution images of the micro-structure of normal luna... In order to study the microstructure characteristics of normal lunate bones, eight fresh cadaver normal lunates were scanned with micro-computed tomography. High-resolution images of the micro-structure of normal lunates were obtained and we analyzed the nutrient foramina. Then nine regions of interest(ROI) were chosen in the central sagittal plane so that we could obtain the parameters of trabecular bones of ROIs. The distal lamellar-like compact structure had statistically significant differences when it was compared with the ROIs in the volar and dorsal ends of the distal cortex. The difference of diameter between the volar and dorsal foramina was significant(P<0.05). However, there was no significant difference regarding the number. The trabecular bones of the volar and dorsal distal ends had lower intensity than those of the distal central subchondral bone plate. The diameters of the nutrient foramina on the volar cortex were larger than those on the dorsal. This research provided more detailed information about microstructure of normal lunate and the nutrient foramina on cortex, and a reference for further study about diseased lunate. 展开更多
关键词 normal lunate trabecular bones micro-computed tomography nutrient foramina
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Spatial-Temporal Sediment Hydrodynamics and Nutrient Loads in Nyanza Gulf, Characterizing Variation in Water Quality
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作者 Angalika W. S. Misigo Seiji Suzuki 《World Journal of Engineering and Technology》 2018年第2期98-115,共18页
Accelerated aging and eutrophication of water resources is a world menace attributed to influx of nutrient rich sediment from its catchment, resulting in poor water quality and shifts in ecological dynamism. Nyanza Gu... Accelerated aging and eutrophication of water resources is a world menace attributed to influx of nutrient rich sediment from its catchment, resulting in poor water quality and shifts in ecological dynamism. Nyanza Gulf is a paramount source of livelihood, portable water, and of service to the rich biodiversity making it indispensable to the entire Lake Victoria watershed ecosystem. This water resource has been deteriorating over the past decades as a consequent of anthropogenic socio-economical activities. This has effectuated an increase in phytoplankton and hydrophyte colonies. The objective of this study was to track the quality and quantity of sediment inundation into the gulf considering the catchment micro-basins processes and influence of human socio-economical activities. Using Quantum Geographic Information System (QGIS) as an interface to Soil and Water Assessment Tool (SWAT) with input of satellite digital elevation model (DEM), local rainfall, soil and land use data sets were utilized to determine the daily variability in sediment and nutrient loads from five major river basins. The SWAT model was successfully calibrated, and the performance validated with observed hydrological and water quality data. The model achieved identification of seasonal water quality budget filling in knowledge gaps about the catchment. River Nyando, Sondu-Miriu, Awach-Kibuon, Awach-Tende and Kibos discharge sediment loads of 3.91, 1.6, 1.18, 1.06 and 0.78 tons/ha respectively. Total suspended solids (TSS) concentration of up to 578mg/L on average daily is discharged by River Awach-Kibuon. This was associated with intense agricultural activities (>54% of the entire basin) on steep slopes (average 12.97) with Acrisols (15%of the basin) soils that is prone erosion. Poorly managed range-bush land that covers about 10% of this basin also contribute significantly to the TSS yield. River Kibos discharge least TSS concentration of 144.43 mg/L in comparison with other rivers mainly due gentle slope falling into a plain, low erodible Cambisols (covers 20% of the basin) and Ferralsols (10%) as well as Nanga forest effect at its exit. River Awach-Tende and Awach-Kibuon on average discharge 1.67 mg/L and 1.58 mg/L respectively of Total Nitrogen (TN) daily. This was linked to intensive farming on poorly managed dominant Phaeozems and Acrisols that are susceptible to leaching. River Sondu-Miriu is the least contributor with a daily average of 1.1101 mg/L dominated with low leached Nitisols. The bay receives highest Total Phosphorus (TP) loads from River Nyando with daily average of 0.3699 mg/L alluded to high biomass production in the basin and Sondu-Miriu least with 0.0288 mg/L. The fluctuation of nutrients and sediment fluxes correlated positively with rainfall events. The long rainfall season with average regular storm events in March to June yield highest monthly loads as compared to short rainfall season (September to November) with isolated intense storm events over a shorter time. The study depicted poor water quality discharged into the gulf throughout the year by the 5 major basins to be above average of conventional ecological healthy basins. 展开更多
关键词 micro-Basin Spatial SEDIMENT nutrient SWAT ANTHROPOGENIC
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Effect of Surface Roughness in Micro-nano Scale on Slotted Waveguide Arrays in Ku-band
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作者 Na LI Peng LI Liwei SONG 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2017年第3期595-603,共9页
Modeling of the roughness in micro-nano scale and its influence have not been fully investigated, however the roughness will cause amplitude and phase errors of the radiating slot, and decrease the precision and effic... Modeling of the roughness in micro-nano scale and its influence have not been fully investigated, however the roughness will cause amplitude and phase errors of the radiating slot, and decrease the precision and efficiency of the SWA in Ku-band. Firstly, the roughness is simulated using the electromechanical coupled(EC) model. The relationship between roughness and the antenna's radiation properties is obtained. For verification, an antenna proto- type is manufactured and tested, and the simulation method is introduced. According to the prototype, a contrasting experiment dealing with the flatness of the radiating plane is conducted to test the simulation method. The advantage of the EC model is validated by comparisons of the EC model and two classical roughness models (sine wave and fractal function), which shows that the EC model gives a more accurate description model for roughness, the maxi- mum error is 13%. The existence of roughness strongly broadens the beamwidth and raises the side-lobe level of SWA, which is 1.2 times greater than the ideal antenna. In addition, effect of the EC model's evaluation indices is investigated, the most affected scale of the roughness is found, which is 1/10 of the working wavelength. The proposed research provides the instruction for antenna designing and manufacturing. 展开更多
关键词 Slotted waveguide arrays - Roughness model micro/nano-scale Amplitude and phase errors Radiationcharacteristics
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Transport patterns of micro nutrient elements from the continental shelf of the East China Sea to the Kuroshio area
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作者 Lu Saiying, Yao Qiao’er and Chen Zhen 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1992年第2期219-230,共12页
On the basis of the in situ data of DO2, pH, SiO2. PO4-P, NO3-N and NO2-N collected in the north of the East China Sea during 1987-1988, the following points are mainly expounded.1.The inorgonic nutrients are obviousl... On the basis of the in situ data of DO2, pH, SiO2. PO4-P, NO3-N and NO2-N collected in the north of the East China Sea during 1987-1988, the following points are mainly expounded.1.The inorgonic nutrients are obviously affected by continent runoff in the north of the East China Sea. Their distributions are characteristic of its distribution of terrigenous materials.2.There are three transport paths of nutrients from the shelf to the Kuroshio area. The first is mixing-diffusing-advec-tion and upwelling process, the process of biology and biochemistry belongs to the second, and the sinking process is the last one.3.The swing of the Kuroshio axis affectes both the range of the migration of substances through mixing-diffusing-advec-tion process and the upwelling degree of the subsurface Kuroshio water to the shelf.4.Most part of the substances sink as macroparticles to the deep layer before reaching the Kuroshio area. 展开更多
关键词 Transport patterns of micro nutrient elements from the continental shelf of the East China Sea to the Kuroshio area
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Effects of Different Organic Manure Applications on the Micro Nutrient Contents of Soil in Different Growing Seasons
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作者 S. Citak S. Sonmez 《Journal of Agricultural Science and Technology》 2011年第3期298-303,共6页
This experiment aims to elaborate the influence of different organic manure applications on soil iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) contents in three successive seasons. Farmyard manure (FM... This experiment aims to elaborate the influence of different organic manure applications on soil iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) contents in three successive seasons. Farmyard manure (FM), chicken manure (CM) and blood meal (BM) and their mixtures and 1 control collectively 18 applications were tested under the open field conditions. All micro nutrients tended to increase with regard to the successive applications some of which were found to be statistically significant to a different extent. Considering the seasonal mean, an increase between the seasons in Fe, Cu and Mn contents were found to be statistically insignificant, whereas the Zn content was found to be statistically significant. CM gave rise to an augmentation in Cu, the Mn and Zn contents, BM also showed a tendency to increase in Fe contents of soils, but we recommend using BM only in small quantities due to the unfavorable features of the material. Summing up, 2.5 ton ha^-1 CM + 0.3 ton ha^-1 BM and 2.5 ton ha^-1 CM + 4.0 ton ha^-1 FM applications gave the better results. 展开更多
关键词 Farmyard manure chicken manure blood meal micro nutrients.
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旱作条件下施肥对牧草种子产量及构成的影响Ⅱ-分期施氮及微量元素对牧草种子产量及构成的影响 被引量:8
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作者 贺晓 李青丰 索全义 《干旱区资源与环境》 CSSCI CSCD 2001年第S1期84-87,共4页
对老芒麦 ( Elymus sibiricus)和诺丹冰草 ( Agropyron desertorum cv.nordan.)种子生产田进行了分期施氮试验和微量元素喷施试验。结果显示 ,春季施氮肥是保证牧草种子获得高产的首要条件 ,而抽穗期再追施一次氮肥可进一步较大幅度地... 对老芒麦 ( Elymus sibiricus)和诺丹冰草 ( Agropyron desertorum cv.nordan.)种子生产田进行了分期施氮试验和微量元素喷施试验。结果显示 ,春季施氮肥是保证牧草种子获得高产的首要条件 ,而抽穗期再追施一次氮肥可进一步较大幅度地提高产量。 0 .0 5浓度的硼、锰、锌、钼在牧草抽穗期喷施后 ,均产生负效应 ,造成种子减产。 展开更多
关键词 老芒麦 诺丹冰草 氮肥 微量元素 种子生产
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土壤-烟叶中微量营养元素对烤烟吸食品质的影响 被引量:4
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作者 徐雪芹 李小兰 +2 位作者 陈志燕 曾德芬 许霭飞 《安徽农业科学》 CAS 2012年第30期14922-14924,14943,共4页
对广西中烟主烟区植烟土壤和烟叶中微量营养元素含量进行分析,并对不同产区的烟叶香味成分进行分析,研究这些元素对烟叶香气品质的影响。结果表明,不同产区土壤和烟叶中营养元素含量存在较大差异,且植烟土壤中元素含量对烟叶中营养元素... 对广西中烟主烟区植烟土壤和烟叶中微量营养元素含量进行分析,并对不同产区的烟叶香味成分进行分析,研究这些元素对烟叶香气品质的影响。结果表明,不同产区土壤和烟叶中营养元素含量存在较大差异,且植烟土壤中元素含量对烟叶中营养元素的含量影响较大,从而对烟叶的香味品质产生影响。根据研究提出了具体的施肥建议:依照不同产区土壤营养元素的实际情况,通过施肥调节土壤营养元素含量,协调烟叶中元素含量,从而有效改善烟叶的香气品质。 展开更多
关键词 烤烟 土壤 中微量营养元素 吸食品质
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毛竹林林下种植中药材对土壤有效态中、微量营养元素的影响
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作者 朱瀚卿 丁苏雅 +4 位作者 马姜明 谭一波 田红灯 申文辉 段敏 《南方农业学报》 CAS CSCD 北大核心 2024年第3期794-802,共9页
【目的】探究毛竹林林下种植中药材对土壤有效态中、微量营养元素的影响,为毛竹林林下种植土壤养分资源管理及我国南方竹产区林药复合经营模式的推广提供科学参考。【方法】以广西桂林漓江源区毛竹人工林林下种植的3种中药材样地为研究... 【目的】探究毛竹林林下种植中药材对土壤有效态中、微量营养元素的影响,为毛竹林林下种植土壤养分资源管理及我国南方竹产区林药复合经营模式的推广提供科学参考。【方法】以广西桂林漓江源区毛竹人工林林下种植的3种中药材样地为研究对象,采用完全随机区组设计,设4个处理,即未种植中药材的毛竹林对照、林下种植黄花倒水莲(Polygala fallax)、林下种植多花黄精(Polygonatum cyrtonema)、林下种植草珊瑚(Sarcandra glabra),在种植后的第2年测定土壤基本理化性质和中、微量营养元素有效态含量,分析其相关性,明确毛竹林林下种植不同中药材土壤中、微量营养元素的变化及其主要影响因素。【结果】毛竹林林下种植中药材降低了土壤容重、总碳和有效磷含量,增加了可溶性有机碳和微生物生物量碳、氮、磷含量,种植多花黄精对上述指标的影响相对较大,除微生物生物量氮外均与对照差异显著(P<0.05,下同);林下种植多花黄精和草珊瑚显著降低了土壤交换性钙和交换性镁含量,而种植黄花倒水莲和草珊瑚显著增加了土壤有效硫含量;林下种植中药材显著增加了土壤有效锌含量,显著降低了土壤有效钼含量,种植多花黄精和草珊瑚也显著增加了土壤有效锰含量,而种植3种中药材对土壤有效铁和有效铜含量无显著影响(P>0.05,下同),种植多花黄精和草珊瑚对土壤微量营养元素有效态含量的影响大于种植黄花倒水莲。相关分析结果表明,土壤中量营养元素钙、镁、硫有效态含量主要受土壤容重、有效磷及微生物生物量碳和磷影响,而微量营养元素铁、锰、铜、锌、钼有效态含量除受以上因素影响外,还与土壤pH及中量营养元素钙和镁有效态含量有关。【结论】土壤中、微量营养元素有效态含量对毛竹林林下种植不同中药材的响应存在较大差异,林下种植多花黄精对土壤养分的影响最大,种植黄花倒水莲和草珊瑚次之。在毛竹林林下种植中药材实践中需适当补充中、微量营养元素,尤其是钙镁肥的供应,且需根据种植中药材的种类采取不同施肥策略。 展开更多
关键词 毛竹林 中药材 土壤养分 中微量元素 影响因素
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基于高肥力设施土壤蔡-18菌液替代化肥对黄瓜生长与土壤生态的影响 被引量:2
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作者 杨小玲 仝雅娜 +3 位作者 华明艳 宋兰芳 马洪英 张淑香 《华北农学报》 CSCD 北大核心 2020年第4期153-160,共8页
为改善设施蔬菜土壤生态,推动化肥零增长计划实施。在土壤速效氮、磷、钾分别为283.55,574.05,1161.3 mg/kg,有机质54.9 g/kg的高肥力日光温室中设置追施水溶性化肥(WSF)、蔡-18菌发酵液(Cai-18)和不施肥(CK)3种施肥处理,采用Miseq高通... 为改善设施蔬菜土壤生态,推动化肥零增长计划实施。在土壤速效氮、磷、钾分别为283.55,574.05,1161.3 mg/kg,有机质54.9 g/kg的高肥力日光温室中设置追施水溶性化肥(WSF)、蔡-18菌发酵液(Cai-18)和不施肥(CK)3种施肥处理,采用Miseq高通量技术、气相色谱-质谱(GC-MS)测定法、顶空固相微萃取-气质联用(HS-SPME-GCMS)分析方法测定3种施肥方式对黄瓜产量和品质、根区土壤养分含量、根际土壤微生物群落结构的影响。结果表明,WSF和Cai-18处理显著提高黄瓜产量,比CK分别增产6.21%和6.59%,Cai-18处理的氮、磷、钾用量比WSF处理分别减少93.48%,95.44%,98.63%,但两者产量差异不显著。Cai-18处理增加黄瓜酯类香气物质的种类和相对含量,提高果实的风味;WSF和Cai-18处理在黄瓜质地和可溶性糖含量差异不大,但二者果实的可溶性糖含量显著高于CK。Cai-18处理根际土壤细菌的97%相似水平下OTU分组数、菌种丰富度指数Chao-1和菌种多样性指数Shannon大于其他2种处理,而其真菌的OTU数和Shannon指数小于其他2种处理,但均没有达到显著差异。在菌群数量结构上,与WSF处理相比,Cai-18处理改善了根际土壤细菌和真菌菌群数量结构的均衡性;与CK相比,Cai-18处理根际土壤的细菌菌群数量结构均衡性更好,但真菌菌群数量结构均衡性较CK差。黄瓜拉秧后,Cai-18处理土壤速效钾含量比WSF处理降低了140.85 mg/kg(P<0.05),但2种处理的有机质、水解氮和有效磷含量差异不显著;CK处理的速效钾、有效磷含量显著低于前2种处理。在肥力高的土壤上施用蔡-18菌发酵液可以改善根际土壤微生物生态环境,缓解过量施肥导致的根层土壤养分失衡问题,提高黄瓜产量和品质。 展开更多
关键词 黄瓜 -18菌发酵液 根际土壤菌群 根区土壤养分 产量 品质
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犬累-卡-佩斯病的组织形态学观察 被引量:3
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作者 马玉捷 刘玥 +3 位作者 郭婧雯 李茗瑜 陈丰伟 汤小朋 《中国兽医杂志》 CAS 北大核心 2012年第11期29-31,I0001,共4页
本试验旨在对临床上患累-卡-佩斯病犬的股骨头组织形态学进行观察,对该病的组织病理学变化进行描述。收集中国农业大学动物医院患有累-卡-佩斯病并采取手术切除治疗的犬的股骨头以及正常犬的股骨头,对其进行大体观察,Micro-CT观察股骨... 本试验旨在对临床上患累-卡-佩斯病犬的股骨头组织形态学进行观察,对该病的组织病理学变化进行描述。收集中国农业大学动物医院患有累-卡-佩斯病并采取手术切除治疗的犬的股骨头以及正常犬的股骨头,对其进行大体观察,Micro-CT观察股骨头三维微观结构,取股骨头负重区组织进行H.E.染色、甲苯胺蓝染色观察病理变化,扫描电镜观察各组股骨头骨显微结构变化。结果显示,病变组较正常组股骨头结构破坏严重,组织结构疏松、易于凿切,髓腔脂肪细胞增大增多,骨小梁在塌陷、溶解的同时,伴有新小梁骨的形成。 展开更多
关键词 --佩斯病 micro—CT 组织形态学 扫描电镜观察结构变化
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miRNA-9过表达对Aβ损伤的PC12细胞的保护作用 被引量:3
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作者 严钢莉 李朝武 +4 位作者 聂海岭 黎逢光 成勇 毛高峰 方煌 《神经损伤与功能重建》 2015年第1期12-15,共4页
目的:探讨micro RNA-9(mi R-9)过表达对β-淀粉样蛋白(Aβ)损伤的PC12细胞及B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关x蛋白(Bax)表达的影响。方法:PC12细胞分为mi R-9过表达组(E组)、空转染对照组(NC组)、不转染的损伤细胞模型组(NT组),采用CC... 目的:探讨micro RNA-9(mi R-9)过表达对β-淀粉样蛋白(Aβ)损伤的PC12细胞及B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关x蛋白(Bax)表达的影响。方法:PC12细胞分为mi R-9过表达组(E组)、空转染对照组(NC组)、不转染的损伤细胞模型组(NT组),采用CCK-8法检测细胞增殖;采用Annexin-V-PE检测细胞凋亡;采用RT-q PCR检测Bcl-2、Bax m RNA的表达,Western-Blot检测Bcl-2、Bax蛋白表达。结果:与NC组及NT组比较,E组的PC12细胞转染48 h后增殖增加,凋亡率降低,Bcl-2表达增加,Bax表达下降(P<0.05)。结论:mi R-9过表达可保护Aβ损伤的PC12细胞。 展开更多
关键词 micro RNA-9 PC12细胞 增殖 凋亡 B淋巴细胞瘤-2 Bcl-2相关x蛋白
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miR-582-5p在唾液腺腺样囊性癌侵袭、转移中的作用 被引量:1
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作者 曾威 卢文辉 +1 位作者 张志利 赵小朋 《中国口腔颌面外科杂志》 CAS 2016年第5期413-418,共6页
目的 :探讨micro RNA-582-5p(miR-582-5p)对唾液腺腺样囊性癌侵袭、转移能力的调控作用。方法 :通过q PCR验证腮腺腺样囊性癌与正常组织中miR-582-5p的表达水平差异,以唾液腺腺样囊性癌肺转移细胞株SACC-83为实验对象,通过脂质体介导,将... 目的 :探讨micro RNA-582-5p(miR-582-5p)对唾液腺腺样囊性癌侵袭、转移能力的调控作用。方法 :通过q PCR验证腮腺腺样囊性癌与正常组织中miR-582-5p的表达水平差异,以唾液腺腺样囊性癌肺转移细胞株SACC-83为实验对象,通过脂质体介导,将miR-582-5p的拟似物(miR-582-5p mimics)转染至SACC-83细胞,升高miR-582-5p的表达水平。采用荧光实时定量PCR(RT-PCR)检测转染前、后SACC-83中miR-582-5p的表达变化,通过Transwell实验检测细胞侵袭、迁移能力的改变,通过荧光素酶报告实验验证靶基因,Western印迹检测miR-582-5p的靶基因整合素FOXC1的表达变化,免疫组织化学检测FOXC1在癌与正常组织中的差异表达,应用SPSS 16.0软件包对所得数据进行t检验或单因素方差分析。结果:miR-582-5p在唾液腺腺样囊性癌组织中低表达,在正常组织中高表达。转染miR-582-5p mimics后的SACC-83中,miR-582-5p的表达显著上调(P<0.001),SACC-83细胞的侵袭迁移能力显著降低(P<0.001)。Western印迹检测结果显示,在SACC-83中升高miR-582-5p的表达水平后,FOXC1表达显著降低。FOXC1在唾液腺腺样囊性癌组织中高表达,在正常组织中低表达。结论 :低表达miR-582-5p有助于维持腺样囊性癌的侵袭、转移特性;调高其表达水平,能有效抑制SACC-83的侵袭、迁移能力。miR-582-5p可能通过调控其靶基因FOXC1的表达而发挥作用。 展开更多
关键词 micro RNA-582-5p 唾液腺 腺样囊性癌 侵袭 转移
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金属尾矿微生物对尾矿环境的适应与调控机制研究进展
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作者 杨正桥 邹奇 +2 位作者 韦行 周凯 陈志良 《生态环境学报》 CSCD 北大核心 2024年第1期156-166,共11页
当前中国金属尾矿产生量和贮存量大、分布广、监管难度大、综合利用率低,由此而引起的环境污染问题引起了广泛关注。研究金属尾矿微生物对尾矿环境的适应和调控机制对于尾矿的生物修复具有重要的现实意义,是加强重金属污染防控的重要组... 当前中国金属尾矿产生量和贮存量大、分布广、监管难度大、综合利用率低,由此而引起的环境污染问题引起了广泛关注。研究金属尾矿微生物对尾矿环境的适应和调控机制对于尾矿的生物修复具有重要的现实意义,是加强重金属污染防控的重要组成部分。综述了金属尾矿微生物群落的物种组成,并从pH、重金属和养分方面阐明其对尾矿环境的适应和调控机制。结果表明,金属尾矿中的优势细菌主要为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和酸杆菌门(Acidobacteria);优势真菌主要为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和罗兹菌门(Rozellomycota);奇古菌门(Thaumarchaeota)和广古菌门(Euryarchaeota)是其中的优势古菌。金属尾矿微生物属于K型策略微生物,它们通过阻止质子和重金属离子进入细胞和从细胞内部排出质子和重金属离子的方式来适应尾矿极酸性和高含量重金属环境,并在养分限制下利用有限的资源获取营养。微生物还进化出了主动改变尾矿环境的能力,它们通过中和质子以及转化和沉淀重金属的方式提高尾矿环境pH值和降低重金属的毒性,并通过固碳、固氮作用和溶解无机磷来积累养分。此外,豆科植物等修复植物能通过分泌次级代谢产物,吸引有益微生物参与尾矿环境的改善。对于金属尾矿的微生物修复研究,可以从金属尾矿风化和修复期间微生物群落的动态变化、多组学技术联合筛选尾矿修复菌剂、微生物的水平基因转移机制、金属尾矿中病毒多样性、微生物之间和微生物与植物的相互作用等方面深入研究,充分发掘金属尾矿微生物在促进生态修复方面的潜力,为金属尾矿环境治理提供科学的解决方案。 展开更多
关键词 金属尾矿 微生物 适应 调控 重金属 养分
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血小板miR-96-5p在血小板储存过程中抗凋亡机制的研究 被引量:1
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作者 徐恒仕 杨英 崔恒祥 《中国医药导报》 CAS 2018年第4期13-17,共5页
目的研究血小板微RNA(micro RNA)基因mi R-96-5p在血小板储存过程中抗凋亡的机制,探讨血小板储存时间的影响因素。方法用定量PCR方法检测储存起始和储存至第5天的健康血液捐献者的单采血小板中的24种与凋亡相关的mi RNAs的表达水平的变... 目的研究血小板微RNA(micro RNA)基因mi R-96-5p在血小板储存过程中抗凋亡的机制,探讨血小板储存时间的影响因素。方法用定量PCR方法检测储存起始和储存至第5天的健康血液捐献者的单采血小板中的24种与凋亡相关的mi RNAs的表达水平的变化,检测结果进行统计学分析,判断mi RNA在调节血小板存活或者凋亡方面的作用。结果与储存起始比较,血小板储存至第5天mi R-96-5p、mi R-16-5p、mi R-155-5p、mi R-148a-5p和mi R-296-5p的表达显著上升(P<0.01),而mi R-7-5p、mi R-145-5p、mi R-15a-5p、mi R-25-3p以及mi R-24-3p的表达显著下降(P<0.01)。转染mi R-96-5p抑制物至血小板72 h后,增加了细胞半胱氨酸蛋白酶-3(caspase-3)的活性,抑制了Bcl-2和Bcl-xl的表达,促进了Bax的积累和活性caspase-3的加工。结论 mi R-96-5p在血小板储存中可能发挥抗凋亡作用。 展开更多
关键词 micro RNA MI R-96-5p 血小板 抗凋亡 CASPASE-3
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转录因子核因子-κB p65通过miR-17调控血管平滑肌细胞增殖的机制研究 被引量:3
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作者 段力仁 杨栋 +5 位作者 张晶 林姝 赵琳 王仑 吕俊远 辛世杰 《中国血管外科杂志(电子版)》 2017年第2期129-132,共4页
目的研究miR-17在血管平滑肌细胞(VSCM)增殖中的作用,以及转录因子核因子(NF)-κB调控miR-17的作用机制。方法将miR-17mimics转染人VSCM,CCK-8法检测细胞活力,流式细胞术检测细胞周期。生物信息学预测NF-κB p65与miR-17启动子区存在结... 目的研究miR-17在血管平滑肌细胞(VSCM)增殖中的作用,以及转录因子核因子(NF)-κB调控miR-17的作用机制。方法将miR-17mimics转染人VSCM,CCK-8法检测细胞活力,流式细胞术检测细胞周期。生物信息学预测NF-κB p65与miR-17启动子区存在结合位点,将pcDNA3.1-p65与报告基因载体p GL3-miR-17启动子区共转染至293T细胞中,通过检测荧光素酶报告基因Luc活性分析NF-κB p65对miR-17启动子区的影响。脂多糖(LPS)刺激细胞后,检测NF-κB p65、miR-17的表达水平。结果与miR-NC组相比,转染miR-17 mimics后,VSMC增殖能力增强,差异具有统计学意义(P<0.05),细胞周期G0/G1期明显减少,S与G2/M明显增加,差异具有统计学意义(P<0.05)。与miR-17启动子区结合位点突变型(Mutant)组相比,转染miR-17启动子区野生型(Wild)组荧光素酶Luc活性增高,差异具有统计学意义(P<0.05)。与对照组相比,LPS刺激细胞后,NF-κB p65、miR-17表达水平明显升高,差异具有统计学意义(P<0.05)。结论 NF-κB通过直接结合miR-17启动子区,促进miR-17的表达从而促进VSMC的增殖。 展开更多
关键词 血管平滑肌细胞 核因子-ΚB P65 micro RNA
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表观遗传学对乳腺癌雌激素受体-α的调控 被引量:2
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作者 王泽涛 吕付龙 王劲松 《实用肿瘤学杂志》 CAS 2014年第3期268-271,共4页
目前,内分泌治疗是激素受体阳性乳腺癌主要的治疗手段,而雌激素受体-α( Estrogen re-ceptor-α,ER-α)是这种治疗的主要靶分子之一。乳腺癌中ER的功能受很多因素影响,如ER基因的改变、微环境的改变、信号转导通路的变化等等,而... 目前,内分泌治疗是激素受体阳性乳腺癌主要的治疗手段,而雌激素受体-α( Estrogen re-ceptor-α,ER-α)是这种治疗的主要靶分子之一。乳腺癌中ER的功能受很多因素影响,如ER基因的改变、微环境的改变、信号转导通路的变化等等,而其中表观遗传学的变化引起ER表达量及功能的改变日益引起重视。在表观遗传学的改变中,ER启动基因的甲基化是发生几率最高的,也是研究最广泛的,它能导致ER表达的下调或缺失。组蛋白修饰也可影响ER-α的表达及功能。而特定micro RNAs( miR-NAs)能调节乳腺癌细胞ER-α的表达并影响乳腺癌对他莫昔芬( Tamoxifen,TAM)反应性。 展开更多
关键词 DNA甲基化 组蛋白乙酰化 组蛋白甲基化 雌激素受体-Α micro RNAS
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Interactive effects of irrigation and exponential fertilization on nutritional characteristics in Populus 3 euramericana cv. ‘74/76' cuttings in an open-air nursery in Beijing, China 被引量:6
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作者 Zi Wang Luyi Ma +2 位作者 Zhongkui Jia Hongxu Wei Jie Duan 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第3期569-582,共14页
Nutritional characteristics determine tree stock quality to a considerable extent. Exponential fertilization can induce nutrient reserves within juvenile trees, but its validity on poplar is contingent on interaction ... Nutritional characteristics determine tree stock quality to a considerable extent. Exponential fertilization can induce nutrient reserves within juvenile trees, but its validity on poplar is contingent on interaction with a scientific irrigation regime due to limited water resources under global warming. In the present study, we raised 3200 Populus 9 euramericana cv. '74/76' cuttings under four irrigation regimes of 0(I0), 60 %(I60), 80 %(I80), and100 %(I100) of field capacity for soil moisture content with or without(control) the employment of nitrogen(N) addition delivered as exponential fertilization at the rate of 8 g N cutting^(-1)(E8) in an open-air nursery in Beijing, China. Both height and diameter increased with the increment of soil moisture ratio or in response to exponential fertilization(EF) without any interactive effects. In general, concentrations of N, phosphorus(P),and potassium(K) declined with time in stem but foliar N concentration did not change. Under the I100 regime, EF increased foliar N concentration relative to the control but failed to affect N concentration in stem in September, when both N concentration and N content were increased by EF under the I80 regime. Stem-K content and concentration by EF under the I80 regime also increased in September,therefore EF-treated cuttings had a higher ratio of K content in stem to that in whole plant(%ANAR). Vector diagnosis for nutritional status indicated that EF resulted in dilution of K concentration but induced a steady-state P uptake in leaves under all irrigation regimes. Therefore, EF of N addition could promote N uptake to leaves of P. 9 euramericana cv. '74/76' cuttings, but it had a null effect on N and P reserves in stem and impaired K reserves.In conclusion, the irrigation regime of 80 % field capacity of soil moisture content was suggested for the culture of juvenile P. 9 euramericana cv. '74/76' cuttings. As the interactive meaning, EF was also suggested for its excellent promotion on both N and K uptakes. 展开更多
关键词 Poplar - Nitrogen Phosphorus POTASSIUM nutrient loading Vector diagnosis
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