Historically, industrial hemp (Cannabis sativa L.) has been a valuable source of metabolites and compounds, such as cannabidiols. There is a need for large amounts of plant tissue to be grown under controlled environm...Historically, industrial hemp (Cannabis sativa L.) has been a valuable source of metabolites and compounds, such as cannabidiols. There is a need for large amounts of plant tissue to be grown under controlled environments, and plant tissue culture is one unique way to yield this tissue. The purposes of this study were to determine: 1) the optimal concentrations (μM)/ratios of auxin:cytokinin in media and;2) the optimal mineral salts formulation for callus induction and callus growth in select hemp cultivars. To find the optimal concentration/ratios, 16 different combinations of auxin:cytokinin and three different mineral salts formulations were evaluated. The three mineral salts formulations tested were MS salts, MB5D1K and an MTSU formulation. The top performing hormone formulations were determined to be equal concentrations (1:1, 2:2, 3:3 μM) of auxin and cytokinin. The top performing media hormone formulations for callus induction were determined to be 2:1, 2:2, 2:3, and 3:2 μM (auxin:cytokinin). The optimal mineral salts formulation was determined to be MD5D1K. Therefore, the overall optimal media formulation for hemp callus production would be MB5D1K salts with the concentration/ratio of 2:2 μM (auxin:cytokinin).展开更多
Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Method...Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Methods.PTH gene polymorphism was detected in270subjects by polymerase chain reaction(PCR)and PCR /restriction fragment length polymorphism(PCR /RFLP).The digestion products of restriction enzyme Bst B1were separated on1%agarose gels.PTH genotypes were confirmed by DNA sequences analysis.BMD was measured by dual-energy X-ray absorptiometry(DEXA,DPX -L ,Lunar).Results.Genotype frequencies of BB,Bb,bb were73.7%,25.9%and0.4%respectively in Beijing adults(P<0.01),67.1%,32.2%and0.7%in postmenopausal women,which was different from Japanese wom-en.We statistically compared bone mineral density at the lumbar2-4and proximal femur between BB and Bb genotypes.No obvious association was found between the BMD and PTH genotypes in Beijing women(P>0.05).Conclusion.PTH gene polymorphism is not associated with BMD in Chinese women.The further research to explore the genetic risk factors of osteoporosis should be committed.展开更多
目的探讨北京南郊中老年人骨代谢指标与骨密度的相关性,以及在骨质疏松症及骨折风险预测中的价值。方法以2017年4月至2023年4月在首都医科大学大兴教学医院行骨密度(bone mineral density,BMD)检查的中老年患者3608例作为研究对象,收集...目的探讨北京南郊中老年人骨代谢指标与骨密度的相关性,以及在骨质疏松症及骨折风险预测中的价值。方法以2017年4月至2023年4月在首都医科大学大兴教学医院行骨密度(bone mineral density,BMD)检查的中老年患者3608例作为研究对象,收集性别、年龄、BMD、25(OH)D_(3)、N-MID、PTH等指标,比较骨代谢指标在不同骨量及性别间的差异,各指标之间的相关性,建立10年主要骨质疏松性骨折风险(FRAX-MOF)和髋部骨折风险(FRAX-HF)回归方程,绘制不同指标诊断骨量减少或骨质疏松的ROC曲线。结果女性FRAX骨折风险高于男性,女性在各骨量组的N-MID高于男性但25(OH)D_(3)含量低于男性,年龄、N-MID与BMD呈负相关(r=-0.87),与MOF、HF呈正相关(年龄:r=0.87和0.96,N-MID:r=0.87和0.77)。BMI与BMD呈正相关(r=0.67),与MOF、HF呈负相关(r=-0.67和-0.70),差异均有统计学意义。构建HF回归方程模型:Y=-1.051+0.057×年龄-0.222×性别-0.041×BMI-0.508×BMD-0.003×N-MID-0.003×25(OH)D_(3),R^(2)=0.724,P=0.000。N-MID预测骨质疏松(T值≤-2.5)和骨量减少(T值<-1)的AUC值为0.62和0.60,差异有统计学意义。结论N-MID与BMD有较好的相关性,在骨质疏松和FRAX-HF的早期识别中可能发挥作用。血清25(OH)D_(3)及PTH对于骨量变化及骨折风险的识别价值,可能更需要结合性别、年龄及基础疾病等情况综合考虑。展开更多
文摘Historically, industrial hemp (Cannabis sativa L.) has been a valuable source of metabolites and compounds, such as cannabidiols. There is a need for large amounts of plant tissue to be grown under controlled environments, and plant tissue culture is one unique way to yield this tissue. The purposes of this study were to determine: 1) the optimal concentrations (μM)/ratios of auxin:cytokinin in media and;2) the optimal mineral salts formulation for callus induction and callus growth in select hemp cultivars. To find the optimal concentration/ratios, 16 different combinations of auxin:cytokinin and three different mineral salts formulations were evaluated. The three mineral salts formulations tested were MS salts, MB5D1K and an MTSU formulation. The top performing hormone formulations were determined to be equal concentrations (1:1, 2:2, 3:3 μM) of auxin and cytokinin. The top performing media hormone formulations for callus induction were determined to be 2:1, 2:2, 2:3, and 3:2 μM (auxin:cytokinin). The optimal mineral salts formulation was determined to be MD5D1K. Therefore, the overall optimal media formulation for hemp callus production would be MB5D1K salts with the concentration/ratio of 2:2 μM (auxin:cytokinin).
文摘Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Methods.PTH gene polymorphism was detected in270subjects by polymerase chain reaction(PCR)and PCR /restriction fragment length polymorphism(PCR /RFLP).The digestion products of restriction enzyme Bst B1were separated on1%agarose gels.PTH genotypes were confirmed by DNA sequences analysis.BMD was measured by dual-energy X-ray absorptiometry(DEXA,DPX -L ,Lunar).Results.Genotype frequencies of BB,Bb,bb were73.7%,25.9%and0.4%respectively in Beijing adults(P<0.01),67.1%,32.2%and0.7%in postmenopausal women,which was different from Japanese wom-en.We statistically compared bone mineral density at the lumbar2-4and proximal femur between BB and Bb genotypes.No obvious association was found between the BMD and PTH genotypes in Beijing women(P>0.05).Conclusion.PTH gene polymorphism is not associated with BMD in Chinese women.The further research to explore the genetic risk factors of osteoporosis should be committed.
文摘目的探讨北京南郊中老年人骨代谢指标与骨密度的相关性,以及在骨质疏松症及骨折风险预测中的价值。方法以2017年4月至2023年4月在首都医科大学大兴教学医院行骨密度(bone mineral density,BMD)检查的中老年患者3608例作为研究对象,收集性别、年龄、BMD、25(OH)D_(3)、N-MID、PTH等指标,比较骨代谢指标在不同骨量及性别间的差异,各指标之间的相关性,建立10年主要骨质疏松性骨折风险(FRAX-MOF)和髋部骨折风险(FRAX-HF)回归方程,绘制不同指标诊断骨量减少或骨质疏松的ROC曲线。结果女性FRAX骨折风险高于男性,女性在各骨量组的N-MID高于男性但25(OH)D_(3)含量低于男性,年龄、N-MID与BMD呈负相关(r=-0.87),与MOF、HF呈正相关(年龄:r=0.87和0.96,N-MID:r=0.87和0.77)。BMI与BMD呈正相关(r=0.67),与MOF、HF呈负相关(r=-0.67和-0.70),差异均有统计学意义。构建HF回归方程模型:Y=-1.051+0.057×年龄-0.222×性别-0.041×BMI-0.508×BMD-0.003×N-MID-0.003×25(OH)D_(3),R^(2)=0.724,P=0.000。N-MID预测骨质疏松(T值≤-2.5)和骨量减少(T值<-1)的AUC值为0.62和0.60,差异有统计学意义。结论N-MID与BMD有较好的相关性,在骨质疏松和FRAX-HF的早期识别中可能发挥作用。血清25(OH)D_(3)及PTH对于骨量变化及骨折风险的识别价值,可能更需要结合性别、年龄及基础疾病等情况综合考虑。
