MicroRNAs(miRNAs)are important regulators in gene expression.The dysregulation of miRNA expression is widely reported in the transformation from physiological to pathological states of cells.A large number of differen...MicroRNAs(miRNAs)are important regulators in gene expression.The dysregulation of miRNA expression is widely reported in the transformation from physiological to pathological states of cells.A large number of differentially expressed miRNAs(DEMs)have been identified in various human cancers by using high-throughput technologies,such as microarray and miRNAseq.Through mining of published studies with high-throughput experiment information,the database of DEMs in human cancers(dbDEMC)was constructed with the aim of providing a systematic resource for the storage and query of the DEMs.Here we report an update of the dbDEMC to version 3.0,which contains two-fold more data entries than the second version and now includes also data from mice and rats.The dbDEMC 3.0 contains 3268 unique DEMs in 40 different cancer types.The current datasets for differential expression analysis have expanded to 9 generalized categories.Moreover,the current release integrates functional annotations of DEMs obtained by using experimentally validated targets.The annotations can be of great benefit to the intensive analysis of the roles of DEMs in cancer.In summary,dbDEMC 3.0 provides a valuable resource for characterizing molecular functions and regulatory mechanisms of DEMs in human cancers.The dbDEMC 3.0 is freely accessible at https://www.biosino.org/dbDEMC.展开更多
A better understanding of genetic bases of growth regulation is essential for bivalve breeding,which is helpful to improve the yield of the commercially important bivalves.While previous studies have identified some c...A better understanding of genetic bases of growth regulation is essential for bivalve breeding,which is helpful to improve the yield of the commercially important bivalves.While previous studies have identified some candidate genes accounting for variation in growth-related traits through genotype-phenotype association analyses,seldom of them have verified the functions of these putative,growth-related genes beyond the genomic level due to the difficulty of culturing commercial bivalves under laboratory conditions.Fortunately,dwarf surf clam Mulinia lateralis can serve as a model organism for studying marine bivalves given its short generation time,the feasibility of being grown under experimental conditions and the availability of genetic and biological information.Using dwarf surf clam as a model bivalve,we characterize E2F3,a gene that has been found to account for variation in growth in scallops by a previous genome-wide association study,and verify its function in growth regulation through RNA interference(RNAi)experiments.For the first time,E2F3 in dwarf surf clam,which is termed as MulE2F3,is characterized.The results reveal that dwarf surf clams with MulE2F3 knocked down exhibit a reduction in both shell size and soft-tissue weight,indicating the functions of MulE2F3 in positively regulating bivalve growth.More importantly,we demonstrate how dwarf surf clam can be used as a model organism to investigate gene functions in commercial bivalves,shedding light on genetic causes for variation in growth to enhance the efficiency of bivalve farming.展开更多
People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component...People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component characteristics of traditional Chinese medicine,it is difficult to elaborate from a single perspective.As a high-quality animal model,Caenorhabditis elegans with the characteristics of small size and fast reproduction,has been widely used in traditional Chinese medicine activity experiments.In this article,Caenorhabditis elegans,as model organisms used to screen the active components of traditional Chinese medicine were reviewed.展开更多
Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein functio...Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein function or structure,understanding their genetic basis is crucial for accurate diagnosis and targeted therapies.To investigate the underlying pathogenesis of these conditions,researchers often use non-mammalian model organisms,such as Drosophila(fruit flies),which is valued for their genetic manipulability,cost-efficiency,and preservation of genes and biological functions across evolutionary time.Genetic tools available in Drosophila,including CRISPR-Cas9,offer a means to manipulate gene expression,allowing for a deep exploration of the genetic underpinnings of rare neurological diseases.Drosophila boasts a versatile genetic toolkit,rapid generation turnover,and ease of large-scale experimentation,making it an invaluable resource for identifying potential drug candidates.Researchers can expose flies carrying disease-associated mutations to various compounds,rapidly pinpointing promising therapeutic agents for further investigation in mammalian models and,ultimately,clinical trials.In this comprehensive review,we explore rare neurological diseases where fly research has significantly contributed to our understanding of their genetic basis,pathophysiology,and potential therapeutic implications.We discuss rare diseases associated with both neuron-expressed and glial-expressed genes.Specific cases include mutations in CDK19 resulting in epilepsy and developmental delay,mutations in TIAM1 leading to a neurodevelopmental disorder with seizures and language delay,and mutations in IRF2BPL causing seizures,a neurodevelopmental disorder with regression,loss of speech,and abnormal movements.And we explore mutations in EMC1 related to cerebellar atrophy,visual impairment,psychomotor retardation,and gain-of-function mutations in ACOX1 causing Mitchell syndrome.Loss-of-function mutations in ACOX1 result in ACOX1 deficiency,characterized by very-long-chain fatty acid accumulation and glial degeneration.Notably,this review highlights how modeling these diseases in Drosophila has provided valuable insights into their pathophysiology,offering a platform for the rapid identification of potential therapeutic interventions.Rare neurological diseases involve a wide range of expression systems,and sometimes common phenotypes can be found among different genes that cause abnormalities in neurons or glia.Furthermore,mutations within the same gene may result in varying functional outcomes,such as complete loss of function,partial loss of function,or gain-of-function mutations.The phenotypes observed in patients can differ significantly,underscoring the complexity of these conditions.In conclusion,Drosophila represents an indispensable and cost-effective tool for investigating rare neurological diseases.By facilitating the modeling of these conditions,Drosophila contributes to a deeper understanding of their genetic basis,pathophysiology,and potential therapies.This approach accelerates the discovery of promising drug candidates,ultimately benefiting patients affected by these complex and understudied diseases.展开更多
This paper first introduces the basic connotation of China’s whole-process engineering consulting.Immediately,analyze the organization model,service procurement model and charging standards of foreign whole-process e...This paper first introduces the basic connotation of China’s whole-process engineering consulting.Immediately,analyze the organization model,service procurement model and charging standards of foreign whole-process engineering consulting(international terminology full-life cycle engineering consultant).Second,discuss the government’s role in the development of engineering consulting from two aspects:service management and market access.Finally,combined with the above analysis,the specific problems faced in the implementation process of the whole process engineering consulting are compared.Provide relevant suggestions on how companies and individuals respond to industry development trends.展开更多
The technology of three-dimensional(3D)printing emerged in the late 1970s and has since undergone considerable development to find numerous applications in mechanical engineering,industrial design,and biomedicine.In b...The technology of three-dimensional(3D)printing emerged in the late 1970s and has since undergone considerable development to find numerous applications in mechanical engineering,industrial design,and biomedicine.In biomedical science,several studies have initially found that 3D printing technology can play an important role in the treatment of diseases in hepatopancreatobiliary surgery.For example,3D printing technology has been applied to create detailed anatomical models of disease organs for preoperative personalized surgical strategies,surgical simulation,intraoperative navigation,medical training,and patient education.Moreover,cancer models have been created using 3D printing technology for the research and selection of chemotherapy drugs.With the aim to clarify the development and application of 3D printing technology in hepatopancreatobiliary surgery,we introduce seven common types of 3D printing technology and review the status of research and application of 3D printing technology in the field of hepatopancreatobiliary surgery.展开更多
Model organisms such as Arabidopsis(Arabidopsis thaliana)and rice(Oryza sativa)have proven essential for efficient scientific discovery and development of new methods.With the diversity of plant lineages,some importan...Model organisms such as Arabidopsis(Arabidopsis thaliana)and rice(Oryza sativa)have proven essential for efficient scientific discovery and development of new methods.With the diversity of plant lineages,some important processes such as C4 photosynthesis are not found in either Arabidopsis or rice,so new model species are needed.Due to their small diploid genomes,short life cycles,self-pollination,small adult statures and prolific seed production,domesticated foxtail millet(Setaria italica)and its wild ancestor,green foxtail(S.viridis),have recently been proposed as novel model species for functional genomics of the Panicoideae,especially for study of C4 photosynthesis.This review outlines the development of these species as model organisms,and discusses current challenges and future potential of a Setaria model.展开更多
Based on the analysis of element geochemistry and total organic carbon(TOC),this study investigates the main factors controlling organic matter(OM)enrichment,reconstructs the evolution process of the sedimentary envir...Based on the analysis of element geochemistry and total organic carbon(TOC),this study investigates the main factors controlling organic matter(OM)enrichment,reconstructs the evolution process of the sedimentary environment,and proposes a dynamic OM enrichment model of the Jurassic Da’anzhai(D)Formation,Sichuan Basin.The results indicate that the Sichuan Basin was generally dominated by a warm and oxidizing sedimentary environment,but with some peculiarities,such as a hotter climate in the D1 member and more anoxic lake water in the D2a member.The sedimentary evolution of the Da’anzhai Formation can be divided into a fluctuating sedimentary stage,a stable sedimentary stage and a reef-building stage.The D2a member showed the strongest hypoxia,the weakest weathering,the largest amount of terrestrial inputs,and the highest TOC content.The TOC is positively correlated with reducing conditions and terrestrial inputs,negatively correlated with weathering.Based on these findings,it is suggested that the global climate in the Early Jurassic period had a complex regional effect and the global oceanic anoxic events of the Toarcian did not spread to the Sichuan Basin.Thus,the anoxic deep water,high terrestrial inputs,and weak weathering were conducive to rapid deposition and preservation of lacustrine OM.展开更多
A mechanistic understanding of biology requires appreciating spatiotemporal aspects of gene expression and its functional implications.Conditional expression allows for (ir)reversible switching of genes on or off,with...A mechanistic understanding of biology requires appreciating spatiotemporal aspects of gene expression and its functional implications.Conditional expression allows for (ir)reversible switching of genes on or off,with the potential of spatial and/or temporal control.This provides a valuable complement to the more often used constitutive gene (in)activation through mutagenesis,providing tools to answer a wider array of research questions across biological disciplines.Spatial and/or temporal control are granted primarily by(combinations of) specific promoters,temperature regimens,compound addition,or illumination.The use of such genetic tool kits is particularly widespread in invertebrate animal models because they can be applied to study biological processes in short time frames and on large scales,using organisms amenable to easy genetic manipulation.Recent years witnessed an exciting expansion and optimization of such tools,of which we provide a comprehensive overview and discussion regarding their use in invertebrates.The mechanism,applicability,benefits,and drawbacks of each of the systems,as well as further developments to be expected in the foreseeable future,are highlighted.展开更多
Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural economy.Although both the bumble and honey bees possess similar and host-restricted gut microbiota,they differ in aspect...Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural economy.Although both the bumble and honey bees possess similar and host-restricted gut microbiota,they differ in aspects of morphology,autonomy,physiology,behavior,and life cycle.The social bee gut bacteria exhibit host specificity that is likely a result of long-term co-evolution.The unique life cycle of bumblebees is key for the acquisition and development of their gut microbiota,and affects the strain-level diversity of the core bacterial species.Studies on bumblebee gut bacteria show that they retain less functional capacity for carbohydrate metabolism compared with that of the honeybee.We discuss the potential roles of the bumblebee gut microbiota against pathogenic threats and the application of host-specific probiotics for bumblebees.Given the advantages of the bumblebee microbiome,including the simple structure and host specificity,and the ease of manipulating bumblebee colonies,we propose that bumblebees may provide a valuable system for understanding the general principles of host-microbe interactions,gut-brain axis,and vertical transmission.展开更多
Leveraging the biocatalytic machinery of living organisms for fabricating functional bioelectronic interfaces,in vivo,defines a new class of micro-biohybrids enabling the seamless integration of technology with living...Leveraging the biocatalytic machinery of living organisms for fabricating functional bioelectronic interfaces,in vivo,defines a new class of micro-biohybrids enabling the seamless integration of technology with living biological systems.Previously,we have demonstrated the in vivo polymerization of conjugated oligomers forming conductors within the structures of plants.Here,we expand this concept by reporting that Hydra,an invertebrate animal,polymerizes the conjugated oligomer ETE-S both within cells that expresses peroxidase activity and within the adhesive material that is secreted to promote underwater surface adhesion.The resulting conjugated polymer forms electronically conducting and electrochemically activeμm-sized domains,which are inter-connected resulting in percolative conduction pathways extending beyond 100μm,that are fully integrated within the Hydra tissue and the secreted mucus.Furthermore,the introduction and in vivo polymerization of ETE-S can be used as a biochemical marker to follow the dynamics of Hydra budding(reproduction)and regeneration.This work paves the way for well-defined self-organized electronics in animal tissue to modulate biological functions and in vivo biofabrication of hybrid functional materials and devices.展开更多
Objective:Under the guidance of model organism Caenorhabditis elegans with fine olfactory system,small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced inju...Objective:Under the guidance of model organism Caenorhabditis elegans with fine olfactory system,small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced injury,and their metabolic pathways were elucidated by enrichment.Methods:Rats were irradiated with 12 Gyγ-rays to establish an acute radiation injury model,and their urine was fingerprinted using UPLC-Q/TOF-MS.Further,under the guidance of Caenorhabditis elegans as olfactory-sensitive model organism,the key differential metabolites in urine were found as biomarkers of radiation-induced injury.Results:After rats were irradiated,the radiation injury urine showed a difference from control(sham-irradiated)urine,which could be distinguished by Caenorhabditis elegans.Based on metabolomics analysis,a total of 21 key differential metabolites with P value<0.05 and fold change either>2 or<0.5 were identified,which can be used as sensitive and reliable biomarkers of radiation-induced injury.The pathways were further enriched,and it was found that disorders of five metabolic pathways,including citric acid cycle and amino acid metabolism,play an important role in radiation-induced injury.Conclusions:Due to radiation injury,the metabolites in urine will change significantly.The study on biomarkers guided by model organism Caenorhabditis elegans provides a new perspective to explain the details of metabolic disorders,and also provides experimental basis for the development of new biological dosimeters.展开更多
With the rapid development of digital earth,smart city,and digital twin technology,the demands of three-dimensional model data’s application is getting higher and higher.These data tend to be multi-objectification,mu...With the rapid development of digital earth,smart city,and digital twin technology,the demands of three-dimensional model data’s application is getting higher and higher.These data tend to be multi-objectification,multi-type,multi-scale,complex spatial relationship,and large amount,which brings great challenges to the efficient organization of them.This paper mainly studies the organization of three-dimensional model data,and the main contributions are as follows:1)A integer coding method of three dimensional multi-scale grid is proposed,which can reduce the four-dimensional(spatial dimension and scale dimension)space into one-dimensional,and has better space and scale clustering characteristics by comparing with various types of grid coding.2)The binary algebra calculation method is proposed to realize the basic spatial relationship calculation of three-dimensional grid,which has higher spatial relationship computing ability than 3D-Geohash method;3)The multi-scale integer coding method is applied to the data organization of three-dimensional city model,and the experiment results show that:it is more efficient and stable than the threedimensional R-tree index and Geohash coding method in the establishment of index and the query of three dimensional space.展开更多
This paper describes a method based on an energy minimizing deformable model applied to the 3D biomechanical modeling of a set of organs considered as regions of interest (ROI) for radiotherapy. The initial model cons...This paper describes a method based on an energy minimizing deformable model applied to the 3D biomechanical modeling of a set of organs considered as regions of interest (ROI) for radiotherapy. The initial model consists of a quadratic surface that is deformed to the exact contour of the ROI by means of the physical properties of a mass-spring system. The exact contour of each ROI is first obtained using a geodesic active contour model. The ROI is then parameterized by the vibration modes resulting from the deformation process. Once each structure has been defined, the method provides a 3D global model including the whole set of ROIs. This model allows one to describe statistically the most significant variations among its structures. Statistical ROI variations among a set of patients or through time can be analyzed. Experimental results are presented using the pelvic zone to simulate anatomical variations among structures and its application in radiotherapy treatment planning.展开更多
Unified representation of spatial earth data is an essential scientific issue.The analysis and mining of interdisciplinary spatial earth data resources can help discover hidden scientific knowledge,and even reveal the...Unified representation of spatial earth data is an essential scientific issue.The analysis and mining of interdisciplinary spatial earth data resources can help discover hidden scientific knowledge,and even reveal the intrinsic relationship among different disciplines.However,the different description methods and inner structures among interdisciplinary spatial earth data bring significant chal-lenges to unified data management and collaborative analysis in earth environment research.To address this issue,this paper pro-poses a unified representation method for interdisciplinary spatial earth data.First,this paper establishes a general metadata model and realizes the unified description of interdisciplinary data.Second,an entity data organization model is presented,which can realize the unified organization of entity data with different inner structures.Finally,we introduce the Spatial Earth Data Format(SEDF),a data format based on HDF5 for implementing the data organization model of interdisciplinary spatial earth data.Data representation experiments and validation are conducted to verify the availability and practicability of the proposed data representa-tion method.The results suggest the powerful ability to represent spatial earth data efficiently and ensure data integrity,which is convenient for data management and application.展开更多
One of the most widespread cellular organelles in nature is cilium,which is found in many unicellular and multicellular organisms.Formerly thought to be a mostly vestigial organelle,the cilium has been discovered in t...One of the most widespread cellular organelles in nature is cilium,which is found in many unicellular and multicellular organisms.Formerly thought to be a mostly vestigial organelle,the cilium has been discovered in the past several decades to play critical motile and sensory roles involved in normal organogenesis during development.The role of cilia has also been implicated in an ever increasing array of seemingly unrelated human diseases,including blindness,kidney cysts,neural tube defects and obesity.In this article we review some of the recent developments in research on cilia,and how defects in ciliogenesis and function can give rise to developmental disorders and disease.展开更多
A number of developed countries are rapidly turning into super-aged societies.Consequently,the demand for reduced surgical invasiveness and enhanced efficiency in the medical field has increased due to the need to red...A number of developed countries are rapidly turning into super-aged societies.Consequently,the demand for reduced surgical invasiveness and enhanced efficiency in the medical field has increased due to the need to reduce the physical burden on older patients and shorten their recovery period.Intelligent surgical robot systems offer high precision,high safety,and reduced invasiveness.This paper presents a review of current intelligent surgical robot systems.The history of robots and three types of intelligent surgical robots are discussed.The problems with current surgical robot systems are then analyzed.Several aspects that should be considered in designing new surgical systems are discussed in detail.The paper ends with a summary of the work and a discussion of future prospects for surgical robot development.展开更多
It is central to biology that sequence conservation suggests functional conservation.Animal longevity is an emergent property of selected traits that integrates capacities to perform physical and mental functions afte...It is central to biology that sequence conservation suggests functional conservation.Animal longevity is an emergent property of selected traits that integrates capacities to perform physical and mental functions after reproductive maturity.Though the yeast SIR2 gene was nominated as a longevity gene based on extended replicative longevity of old mother cells,this is not a selected trait:SIR2 is selected against in chronological aging and the direct targets of SIR2 in replicative lifespan are not conserved.Though it would be difficult to imagine how a gene that advantages 1 in 5 million yeast cells could have anticipated causes of aging in animals,overexpression of SIR2 homologs was tested in invertebrates for longevity.Because artifactual positive results were reported years before they were sorted out and because it was not known that SIR2 functions as a pro-aging gene in yeast chronological aging and in flies subject to amino acid deprivation,a global pursuit of longevity phenotypes was driven by a mixture of framing bias,confirmation bias,and hype.Review articles that propagate these biases are so rampant that few investigators have considered how weak the case ever was for sirtuins as longevity genes.Acknowledging that a few positive associations between sirtuins and longevity have been identified after thousands of person-years and billions of dollars of effort,we review the data and suggest rejection of the notions that sirtuins(i)have any specific connection to lifespan in animals and(ii)are primary mediators of the beneficial effects of NAD repletion.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 91959106, 31871255, 9173131081827901)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No.XDB38030100)the Shanghai Municipal Science and Technology (Grant No. 2017SHZDZX01)
文摘MicroRNAs(miRNAs)are important regulators in gene expression.The dysregulation of miRNA expression is widely reported in the transformation from physiological to pathological states of cells.A large number of differentially expressed miRNAs(DEMs)have been identified in various human cancers by using high-throughput technologies,such as microarray and miRNAseq.Through mining of published studies with high-throughput experiment information,the database of DEMs in human cancers(dbDEMC)was constructed with the aim of providing a systematic resource for the storage and query of the DEMs.Here we report an update of the dbDEMC to version 3.0,which contains two-fold more data entries than the second version and now includes also data from mice and rats.The dbDEMC 3.0 contains 3268 unique DEMs in 40 different cancer types.The current datasets for differential expression analysis have expanded to 9 generalized categories.Moreover,the current release integrates functional annotations of DEMs obtained by using experimentally validated targets.The annotations can be of great benefit to the intensive analysis of the roles of DEMs in cancer.In summary,dbDEMC 3.0 provides a valuable resource for characterizing molecular functions and regulatory mechanisms of DEMs in human cancers.The dbDEMC 3.0 is freely accessible at https://www.biosino.org/dbDEMC.
基金supported by the National Natural Science Foundation of China(No.U2106231)the National Key R&D Program of China(No.2022YFD2400303)the Key R&D Project of Shandong Province(No.2022 TZXD003).
文摘A better understanding of genetic bases of growth regulation is essential for bivalve breeding,which is helpful to improve the yield of the commercially important bivalves.While previous studies have identified some candidate genes accounting for variation in growth-related traits through genotype-phenotype association analyses,seldom of them have verified the functions of these putative,growth-related genes beyond the genomic level due to the difficulty of culturing commercial bivalves under laboratory conditions.Fortunately,dwarf surf clam Mulinia lateralis can serve as a model organism for studying marine bivalves given its short generation time,the feasibility of being grown under experimental conditions and the availability of genetic and biological information.Using dwarf surf clam as a model bivalve,we characterize E2F3,a gene that has been found to account for variation in growth in scallops by a previous genome-wide association study,and verify its function in growth regulation through RNA interference(RNAi)experiments.For the first time,E2F3 in dwarf surf clam,which is termed as MulE2F3,is characterized.The results reveal that dwarf surf clams with MulE2F3 knocked down exhibit a reduction in both shell size and soft-tissue weight,indicating the functions of MulE2F3 in positively regulating bivalve growth.More importantly,we demonstrate how dwarf surf clam can be used as a model organism to investigate gene functions in commercial bivalves,shedding light on genetic causes for variation in growth to enhance the efficiency of bivalve farming.
基金This work was financially supported by Scientific Research Fund Project of Education Department of Liaoning Province(LJKZ0960)Science Foundation of Shenyang Pharmaceutical University(GGJJ2021105).
文摘People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component characteristics of traditional Chinese medicine,it is difficult to elaborate from a single perspective.As a high-quality animal model,Caenorhabditis elegans with the characteristics of small size and fast reproduction,has been widely used in traditional Chinese medicine activity experiments.In this article,Caenorhabditis elegans,as model organisms used to screen the active components of traditional Chinese medicine were reviewed.
基金supported by Warren Alpert Foundation and Houston Methodist Academic Institute Laboratory Operating Fund(to HLC).
文摘Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein function or structure,understanding their genetic basis is crucial for accurate diagnosis and targeted therapies.To investigate the underlying pathogenesis of these conditions,researchers often use non-mammalian model organisms,such as Drosophila(fruit flies),which is valued for their genetic manipulability,cost-efficiency,and preservation of genes and biological functions across evolutionary time.Genetic tools available in Drosophila,including CRISPR-Cas9,offer a means to manipulate gene expression,allowing for a deep exploration of the genetic underpinnings of rare neurological diseases.Drosophila boasts a versatile genetic toolkit,rapid generation turnover,and ease of large-scale experimentation,making it an invaluable resource for identifying potential drug candidates.Researchers can expose flies carrying disease-associated mutations to various compounds,rapidly pinpointing promising therapeutic agents for further investigation in mammalian models and,ultimately,clinical trials.In this comprehensive review,we explore rare neurological diseases where fly research has significantly contributed to our understanding of their genetic basis,pathophysiology,and potential therapeutic implications.We discuss rare diseases associated with both neuron-expressed and glial-expressed genes.Specific cases include mutations in CDK19 resulting in epilepsy and developmental delay,mutations in TIAM1 leading to a neurodevelopmental disorder with seizures and language delay,and mutations in IRF2BPL causing seizures,a neurodevelopmental disorder with regression,loss of speech,and abnormal movements.And we explore mutations in EMC1 related to cerebellar atrophy,visual impairment,psychomotor retardation,and gain-of-function mutations in ACOX1 causing Mitchell syndrome.Loss-of-function mutations in ACOX1 result in ACOX1 deficiency,characterized by very-long-chain fatty acid accumulation and glial degeneration.Notably,this review highlights how modeling these diseases in Drosophila has provided valuable insights into their pathophysiology,offering a platform for the rapid identification of potential therapeutic interventions.Rare neurological diseases involve a wide range of expression systems,and sometimes common phenotypes can be found among different genes that cause abnormalities in neurons or glia.Furthermore,mutations within the same gene may result in varying functional outcomes,such as complete loss of function,partial loss of function,or gain-of-function mutations.The phenotypes observed in patients can differ significantly,underscoring the complexity of these conditions.In conclusion,Drosophila represents an indispensable and cost-effective tool for investigating rare neurological diseases.By facilitating the modeling of these conditions,Drosophila contributes to a deeper understanding of their genetic basis,pathophysiology,and potential therapies.This approach accelerates the discovery of promising drug candidates,ultimately benefiting patients affected by these complex and understudied diseases.
文摘This paper first introduces the basic connotation of China’s whole-process engineering consulting.Immediately,analyze the organization model,service procurement model and charging standards of foreign whole-process engineering consulting(international terminology full-life cycle engineering consultant).Second,discuss the government’s role in the development of engineering consulting from two aspects:service management and market access.Finally,combined with the above analysis,the specific problems faced in the implementation process of the whole process engineering consulting are compared.Provide relevant suggestions on how companies and individuals respond to industry development trends.
基金This work was was supported by the National Natural Science Foundation of China(Nos.82270599 and 81902431).
文摘The technology of three-dimensional(3D)printing emerged in the late 1970s and has since undergone considerable development to find numerous applications in mechanical engineering,industrial design,and biomedicine.In biomedical science,several studies have initially found that 3D printing technology can play an important role in the treatment of diseases in hepatopancreatobiliary surgery.For example,3D printing technology has been applied to create detailed anatomical models of disease organs for preoperative personalized surgical strategies,surgical simulation,intraoperative navigation,medical training,and patient education.Moreover,cancer models have been created using 3D printing technology for the research and selection of chemotherapy drugs.With the aim to clarify the development and application of 3D printing technology in hepatopancreatobiliary surgery,we introduce seven common types of 3D printing technology and review the status of research and application of 3D printing technology in the field of hepatopancreatobiliary surgery.
基金supported by Fundamental Research Funds of CAAS(2014ZL002)National High Technology Research and Development Program of China(2013AA102603)+1 种基金the National Natural Science Foundation of China(31171560,31301328)China Agricultural Research System(CARS07-12.5-A02).
文摘Model organisms such as Arabidopsis(Arabidopsis thaliana)and rice(Oryza sativa)have proven essential for efficient scientific discovery and development of new methods.With the diversity of plant lineages,some important processes such as C4 photosynthesis are not found in either Arabidopsis or rice,so new model species are needed.Due to their small diploid genomes,short life cycles,self-pollination,small adult statures and prolific seed production,domesticated foxtail millet(Setaria italica)and its wild ancestor,green foxtail(S.viridis),have recently been proposed as novel model species for functional genomics of the Panicoideae,especially for study of C4 photosynthesis.This review outlines the development of these species as model organisms,and discusses current challenges and future potential of a Setaria model.
基金he Science and Technology Cooperation Project of the CNPC-SWPU Innovation Alliance(NO.2020CX050000)the Research and Innovation Fund for Graduate Students of Southwest Petroleum University(NO.2019cxyb005)。
文摘Based on the analysis of element geochemistry and total organic carbon(TOC),this study investigates the main factors controlling organic matter(OM)enrichment,reconstructs the evolution process of the sedimentary environment,and proposes a dynamic OM enrichment model of the Jurassic Da’anzhai(D)Formation,Sichuan Basin.The results indicate that the Sichuan Basin was generally dominated by a warm and oxidizing sedimentary environment,but with some peculiarities,such as a hotter climate in the D1 member and more anoxic lake water in the D2a member.The sedimentary evolution of the Da’anzhai Formation can be divided into a fluctuating sedimentary stage,a stable sedimentary stage and a reef-building stage.The D2a member showed the strongest hypoxia,the weakest weathering,the largest amount of terrestrial inputs,and the highest TOC content.The TOC is positively correlated with reducing conditions and terrestrial inputs,negatively correlated with weathering.Based on these findings,it is suggested that the global climate in the Early Jurassic period had a complex regional effect and the global oceanic anoxic events of the Toarcian did not spread to the Sichuan Basin.Thus,the anoxic deep water,high terrestrial inputs,and weak weathering were conducive to rapid deposition and preservation of lacustrine OM.
基金supported by Horizon 2020 grant 633589FWO Flanders grant G052217NKU Leuven grant C16/19/003。
文摘A mechanistic understanding of biology requires appreciating spatiotemporal aspects of gene expression and its functional implications.Conditional expression allows for (ir)reversible switching of genes on or off,with the potential of spatial and/or temporal control.This provides a valuable complement to the more often used constitutive gene (in)activation through mutagenesis,providing tools to answer a wider array of research questions across biological disciplines.Spatial and/or temporal control are granted primarily by(combinations of) specific promoters,temperature regimens,compound addition,or illumination.The use of such genetic tool kits is particularly widespread in invertebrate animal models because they can be applied to study biological processes in short time frames and on large scales,using organisms amenable to easy genetic manipulation.Recent years witnessed an exciting expansion and optimization of such tools,of which we provide a comprehensive overview and discussion regarding their use in invertebrates.The mechanism,applicability,benefits,and drawbacks of each of the systems,as well as further developments to be expected in the foreseeable future,are highlighted.
基金This work was supported by the National Key R&D Pro-gram of China(Grant No.2019YFA0906500)National Natural Science Foundation of China Project 32170495.
文摘Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural economy.Although both the bumble and honey bees possess similar and host-restricted gut microbiota,they differ in aspects of morphology,autonomy,physiology,behavior,and life cycle.The social bee gut bacteria exhibit host specificity that is likely a result of long-term co-evolution.The unique life cycle of bumblebees is key for the acquisition and development of their gut microbiota,and affects the strain-level diversity of the core bacterial species.Studies on bumblebee gut bacteria show that they retain less functional capacity for carbohydrate metabolism compared with that of the honeybee.We discuss the potential roles of the bumblebee gut microbiota against pathogenic threats and the application of host-specific probiotics for bumblebees.Given the advantages of the bumblebee microbiome,including the simple structure and host specificity,and the ease of manipulating bumblebee colonies,we propose that bumblebees may provide a valuable system for understanding the general principles of host-microbe interactions,gut-brain axis,and vertical transmission.
基金innovation programme under grant agreement No 800926(FET-OPEN-HyPhOE)by the Swedish Research Council(VR-2017-04910)+5 种基金Additional funding was provided by the Knut and Alice Wallenberg Foundationthe Swedish Foundation for Strategic Research(SSF)The European Research Council(ERC)project e-NeuroPharma 834677the Swedish Government Strategic Research Area in Materials Science on Advanced Functional Materials at Linkoping University(Faculty Grant SFO-Mat-LiU No.2009-00971)supported by MultiPark-A Strategic Research Area at Lund Universitythe MIUR project SHARID-ARS01-01270 for financial support.
文摘Leveraging the biocatalytic machinery of living organisms for fabricating functional bioelectronic interfaces,in vivo,defines a new class of micro-biohybrids enabling the seamless integration of technology with living biological systems.Previously,we have demonstrated the in vivo polymerization of conjugated oligomers forming conductors within the structures of plants.Here,we expand this concept by reporting that Hydra,an invertebrate animal,polymerizes the conjugated oligomer ETE-S both within cells that expresses peroxidase activity and within the adhesive material that is secreted to promote underwater surface adhesion.The resulting conjugated polymer forms electronically conducting and electrochemically activeμm-sized domains,which are inter-connected resulting in percolative conduction pathways extending beyond 100μm,that are fully integrated within the Hydra tissue and the secreted mucus.Furthermore,the introduction and in vivo polymerization of ETE-S can be used as a biochemical marker to follow the dynamics of Hydra budding(reproduction)and regeneration.This work paves the way for well-defined self-organized electronics in animal tissue to modulate biological functions and in vivo biofabrication of hybrid functional materials and devices.
基金This work was supported by the grants from the National NaturalScience Foundation of China(Nos.81572969 and 81730086).
文摘Objective:Under the guidance of model organism Caenorhabditis elegans with fine olfactory system,small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced injury,and their metabolic pathways were elucidated by enrichment.Methods:Rats were irradiated with 12 Gyγ-rays to establish an acute radiation injury model,and their urine was fingerprinted using UPLC-Q/TOF-MS.Further,under the guidance of Caenorhabditis elegans as olfactory-sensitive model organism,the key differential metabolites in urine were found as biomarkers of radiation-induced injury.Results:After rats were irradiated,the radiation injury urine showed a difference from control(sham-irradiated)urine,which could be distinguished by Caenorhabditis elegans.Based on metabolomics analysis,a total of 21 key differential metabolites with P value<0.05 and fold change either>2 or<0.5 were identified,which can be used as sensitive and reliable biomarkers of radiation-induced injury.The pathways were further enriched,and it was found that disorders of five metabolic pathways,including citric acid cycle and amino acid metabolism,play an important role in radiation-induced injury.Conclusions:Due to radiation injury,the metabolites in urine will change significantly.The study on biomarkers guided by model organism Caenorhabditis elegans provides a new perspective to explain the details of metabolic disorders,and also provides experimental basis for the development of new biological dosimeters.
基金National Key R&D Program of China[Grant Number 2018YFB0505304]National Natural Science Foundation of China[Grant Number 41671409].
文摘With the rapid development of digital earth,smart city,and digital twin technology,the demands of three-dimensional model data’s application is getting higher and higher.These data tend to be multi-objectification,multi-type,multi-scale,complex spatial relationship,and large amount,which brings great challenges to the efficient organization of them.This paper mainly studies the organization of three-dimensional model data,and the main contributions are as follows:1)A integer coding method of three dimensional multi-scale grid is proposed,which can reduce the four-dimensional(spatial dimension and scale dimension)space into one-dimensional,and has better space and scale clustering characteristics by comparing with various types of grid coding.2)The binary algebra calculation method is proposed to realize the basic spatial relationship calculation of three-dimensional grid,which has higher spatial relationship computing ability than 3D-Geohash method;3)The multi-scale integer coding method is applied to the data organization of three-dimensional city model,and the experiment results show that:it is more efficient and stable than the threedimensional R-tree index and Geohash coding method in the establishment of index and the query of three dimensional space.
基金Project partially supported by the VI FP and VII FP of the European Commission through MAESTRO and ENVISIONprojects (Nos. IP CE503564 and SP CE241851)Spanish Junta de Comunidades de Castilla–La Mancha (Nos. PBC06-0019 and PI-2006/01.1)
文摘This paper describes a method based on an energy minimizing deformable model applied to the 3D biomechanical modeling of a set of organs considered as regions of interest (ROI) for radiotherapy. The initial model consists of a quadratic surface that is deformed to the exact contour of the ROI by means of the physical properties of a mass-spring system. The exact contour of each ROI is first obtained using a geodesic active contour model. The ROI is then parameterized by the vibration modes resulting from the deformation process. Once each structure has been defined, the method provides a 3D global model including the whole set of ROIs. This model allows one to describe statistically the most significant variations among its structures. Statistical ROI variations among a set of patients or through time can be analyzed. Experimental results are presented using the pelvic zone to simulate anatomical variations among structures and its application in radiotherapy treatment planning.
基金This work was supported by Open Science-oriented Interoperable Global Earth Observation System of Systems(grant number 2019YFE0126400)Programme of Cooperation on the Analysis of Carbon Satellites Data(grant number 131211KYSB20180002).
文摘Unified representation of spatial earth data is an essential scientific issue.The analysis and mining of interdisciplinary spatial earth data resources can help discover hidden scientific knowledge,and even reveal the intrinsic relationship among different disciplines.However,the different description methods and inner structures among interdisciplinary spatial earth data bring significant chal-lenges to unified data management and collaborative analysis in earth environment research.To address this issue,this paper pro-poses a unified representation method for interdisciplinary spatial earth data.First,this paper establishes a general metadata model and realizes the unified description of interdisciplinary data.Second,an entity data organization model is presented,which can realize the unified organization of entity data with different inner structures.Finally,we introduce the Spatial Earth Data Format(SEDF),a data format based on HDF5 for implementing the data organization model of interdisciplinary spatial earth data.Data representation experiments and validation are conducted to verify the availability and practicability of the proposed data representa-tion method.The results suggest the powerful ability to represent spatial earth data efficiently and ensure data integrity,which is convenient for data management and application.
文摘One of the most widespread cellular organelles in nature is cilium,which is found in many unicellular and multicellular organisms.Formerly thought to be a mostly vestigial organelle,the cilium has been discovered in the past several decades to play critical motile and sensory roles involved in normal organogenesis during development.The role of cilia has also been implicated in an ever increasing array of seemingly unrelated human diseases,including blindness,kidney cysts,neural tube defects and obesity.In this article we review some of the recent developments in research on cilia,and how defects in ciliogenesis and function can give rise to developmental disorders and disease.
基金This work was supported in part by the National Natural Science Foundation of China and Shenzhen Robot Research Program(No.U1613221)in part by the National Key Research and Development Program(No.2017YFC0110500)+1 种基金in part by the Global Centers of Excellence Program“Global Robot Academia,”Waseda University,Tokyo,Japanand in part by the Program for Leading Graduate Schools,“Graduate Program for Embodiment Informatics”of the Ministry of Education,Culture,Sports,Science and Technology。
文摘A number of developed countries are rapidly turning into super-aged societies.Consequently,the demand for reduced surgical invasiveness and enhanced efficiency in the medical field has increased due to the need to reduce the physical burden on older patients and shorten their recovery period.Intelligent surgical robot systems offer high precision,high safety,and reduced invasiveness.This paper presents a review of current intelligent surgical robot systems.The history of robots and three types of intelligent surgical robots are discussed.The problems with current surgical robot systems are then analyzed.Several aspects that should be considered in designing new surgical systems are discussed in detail.The paper ends with a summary of the work and a discussion of future prospects for surgical robot development.
基金supported by R01HL147545 and the Alfred E.Mann Family Foundation.
文摘It is central to biology that sequence conservation suggests functional conservation.Animal longevity is an emergent property of selected traits that integrates capacities to perform physical and mental functions after reproductive maturity.Though the yeast SIR2 gene was nominated as a longevity gene based on extended replicative longevity of old mother cells,this is not a selected trait:SIR2 is selected against in chronological aging and the direct targets of SIR2 in replicative lifespan are not conserved.Though it would be difficult to imagine how a gene that advantages 1 in 5 million yeast cells could have anticipated causes of aging in animals,overexpression of SIR2 homologs was tested in invertebrates for longevity.Because artifactual positive results were reported years before they were sorted out and because it was not known that SIR2 functions as a pro-aging gene in yeast chronological aging and in flies subject to amino acid deprivation,a global pursuit of longevity phenotypes was driven by a mixture of framing bias,confirmation bias,and hype.Review articles that propagate these biases are so rampant that few investigators have considered how weak the case ever was for sirtuins as longevity genes.Acknowledging that a few positive associations between sirtuins and longevity have been identified after thousands of person-years and billions of dollars of effort,we review the data and suggest rejection of the notions that sirtuins(i)have any specific connection to lifespan in animals and(ii)are primary mediators of the beneficial effects of NAD repletion.
