Microwave-assisted extraction(MAE)technology was used to extract silymarin from milk thistle seeds.The single factor experiments and quadratic orthogonal rotation regression combination design were adopted to study th...Microwave-assisted extraction(MAE)technology was used to extract silymarin from milk thistle seeds.The single factor experiments and quadratic orthogonal rotation regression combination design were adopted to study the effects of such factors as extraction time,extraction temperature,ethanol concentration and solid-liquid ratio on silymarin yield.Ultraviolet-Visible(UV-Vis)spectrophotometry method was employed to measure the silybin content.The optimal technological conditions for extracting silymarin from milk thistle seeds by MAE were determined,and then the extraction regression mathematical model was established.The importance orders of factors that influenced the extraction yield within the experimental model were as follows:ethanol concentration,the ratio of solid to liquid,extraction temperature and extraction time.The optimum extraction parameters were obtained as follows:extraction time 50 min,extraction temperature 130℃,ethanol concentration 85%(V/V),solid to liquid ratio 1∶40(g/mL),and under such conditions,experimental value of silymarin yield after being extracted three times is 59.33mg/g.The validation experiments verify that the established regression mathematical model can predict the extraction yield of silymarin accurately.展开更多
文摘Microwave-assisted extraction(MAE)technology was used to extract silymarin from milk thistle seeds.The single factor experiments and quadratic orthogonal rotation regression combination design were adopted to study the effects of such factors as extraction time,extraction temperature,ethanol concentration and solid-liquid ratio on silymarin yield.Ultraviolet-Visible(UV-Vis)spectrophotometry method was employed to measure the silybin content.The optimal technological conditions for extracting silymarin from milk thistle seeds by MAE were determined,and then the extraction regression mathematical model was established.The importance orders of factors that influenced the extraction yield within the experimental model were as follows:ethanol concentration,the ratio of solid to liquid,extraction temperature and extraction time.The optimum extraction parameters were obtained as follows:extraction time 50 min,extraction temperature 130℃,ethanol concentration 85%(V/V),solid to liquid ratio 1∶40(g/mL),and under such conditions,experimental value of silymarin yield after being extracted three times is 59.33mg/g.The validation experiments verify that the established regression mathematical model can predict the extraction yield of silymarin accurately.
