phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the comple...phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the complexity of the involved immune components.In the present study,we measured the synchronous variations of PHA response,the proportion of different subtypes of leukocytes,as well as serum bactericidal capacity in circulation blood at 6,12 and 24 h after PHA versus PBS injection in striped hamster,Cricetulus barabensis.First,the results showed that PHA responses reached a peak at 6 h postinjection,then sharply declined at 12 h and 24 h postinjection.Serum bactericidal capacity was higher at 6 h and 12 h than at 24 h.The proportion of different subtypes of leukocytes,as well as the ratio of neutrophils to lymphocytes did not display significant changes across different time points.Second,PHA response was positively correlated with the proportion of neutrophils and serum bactericidal capacity.The proportion of monocytes was negatively correlated with that of eosinophils and neutrophils.The proportion of basophils was negatively correlated with that of lymphocytes.Our results indicate that earlier enhanced PHA response is important for the striped hamster to cope with changing environmental conditions due to its small body mass,and the increased components of innate immunity in circulation blood may contribute to the enhancement of PHA swelling response.展开更多
CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 μg/ml)...CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 μg/ml) were treated by CD134 mAb (1 μg/ml, 5 μg/ml, 10 μg/ml) for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 μg/ml to 5 μg/ml and dropped down to its minimum on 24 h (p ＜ 0.05). The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 μg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival.展开更多
目的观察乳康饮(Ru Kang Yin,RKY)刺激γδT细胞增殖对乳腺癌细胞MDA-MB-231的杀伤作用,为中医药治疗乳腺癌提供依据。方法实验分为以下4组,Ⅰ组:外周血单个核细胞(PBMC)对照组;Ⅱ组:PBMC+植物凝集素(PHA)组;Ⅲ组:PBMC+唑来膦酸(ZOL)组...目的观察乳康饮(Ru Kang Yin,RKY)刺激γδT细胞增殖对乳腺癌细胞MDA-MB-231的杀伤作用,为中医药治疗乳腺癌提供依据。方法实验分为以下4组,Ⅰ组:外周血单个核细胞(PBMC)对照组;Ⅱ组:PBMC+植物凝集素(PHA)组;Ⅲ组:PBMC+唑来膦酸(ZOL)组;Ⅳ组:PBMC+RKY组。在各组培养0 d和14 d时,用流式细胞术检测各组γδT细胞占PBMC的百分比。将用免疫磁珠分选的γδT细胞与荧光染料羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)标记的乳腺癌MDA-MB-231细胞以10∶1的比例共培养,测定γδT细胞对乳腺癌MDA-MB-231细胞的杀伤力。结果培养0 d时,Ⅰ组~Ⅳ组γδT细胞占PBMC百分比分别为(3.81±0.27)%、(4.19±0.41)%、(3.94±0.13)%、(4.16±0.11)%,各组间差异无统计学意义(F=1.462,P=0.296)。培养14 d时,Ⅰ组~Ⅳ组γδT细胞占PBMC百分比分别为(4.70±0.29)%、(31.09±1.95)%、(25.91±3.77)%、(28.84±2.54)%,各组间差异有统计学意义(F=71.985,P=0.000)。Ⅰ组~Ⅳ组对MDA-MB-231细胞的杀伤率分别为(1.17±0.86)%、(1.56±0.13)%、(1.47±0.09)%、(2.01±0.16)%,各组间差异有统计学意义(F=24.649,P=0.000)。结论RKY可以刺激γδT细胞增殖,提高对MDA-MB-231细胞的杀伤力,为乳腺癌的中医药治疗提供实验依据。展开更多
基金This study was supported by a Key Project of Natural Science of the Anhui Province Department of Education,China(KJ2011A114)supported by the State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,The Chinese Academy of Sciences(ChineseIPM1001)to ZZQ.
文摘phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the complexity of the involved immune components.In the present study,we measured the synchronous variations of PHA response,the proportion of different subtypes of leukocytes,as well as serum bactericidal capacity in circulation blood at 6,12 and 24 h after PHA versus PBS injection in striped hamster,Cricetulus barabensis.First,the results showed that PHA responses reached a peak at 6 h postinjection,then sharply declined at 12 h and 24 h postinjection.Serum bactericidal capacity was higher at 6 h and 12 h than at 24 h.The proportion of different subtypes of leukocytes,as well as the ratio of neutrophils to lymphocytes did not display significant changes across different time points.Second,PHA response was positively correlated with the proportion of neutrophils and serum bactericidal capacity.The proportion of monocytes was negatively correlated with that of eosinophils and neutrophils.The proportion of basophils was negatively correlated with that of lymphocytes.Our results indicate that earlier enhanced PHA response is important for the striped hamster to cope with changing environmental conditions due to its small body mass,and the increased components of innate immunity in circulation blood may contribute to the enhancement of PHA swelling response.
文摘CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 μg/ml) were treated by CD134 mAb (1 μg/ml, 5 μg/ml, 10 μg/ml) for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 μg/ml to 5 μg/ml and dropped down to its minimum on 24 h (p ＜ 0.05). The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 μg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival.
