Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, ...Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, we investigated the effect of arsenic on agonist-induced vasorelaxation and vasoconstriction using the isolated rat aortic rings in in vitro organ bath system. Treatment with inorganic arsenite (AsⅢ) inhibited acetylcholine-induced relaxation of aortic rings by inhibiting production of nitric oxide in endothelium.展开更多
In 1995,Huang et al.reported that rhodocytin,asnake toxin purified from callosdasma rhodostoma venomstimulates platelet aggregation.Ten years later,Suzuki-Inoue et al.identified C-type lectin-like receptor 2(CLEC-2)on...In 1995,Huang et al.reported that rhodocytin,asnake toxin purified from callosdasma rhodostoma venomstimulates platelet aggregation.Ten years later,Suzuki-Inoue et al.identified C-type lectin-like receptor 2(CLEC-2)on platelets as the rhodocytin receptor.Thereafter,several studies have showed that platelet CLEC-2 isinvolved in lymphatic/blood vessel separation,tumormetastasis and thrombus formation.展开更多
BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final e...BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final effect.In order to reveal the compatibility mechanism of BHD's prescription in treating and preventing ischemic cerebrovascular disease,we needed explore the effect and relation of ingredients in the prescription.OBJECTIVE:To observe the effect of Buyang Huanwu decoction(BHD)and Astragalus mongholicus on the activity of platelet activating factor receptor(PAFR)in the platelet of rabbits in vitro,and investigate the mechanism of Astragalus mongholicus.DESIGN:A decomposed recipes study.SETTING:Guangzhou University of Traditional Chinese Medicine.MATERIALS:Five New Zealand rabbits,weighing 2-3 kg,both sexes,were used.BHD was composed of Sheng Huang Qi 120 g,Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g,Hong Hua 3 g.The prescription for activating blood circulation consisted of Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g and Hong Hua 3 g.The prescription for invigorating qi consisted of 120 g Sheng Huang Qi.The prepared herbal pieces were purchased from the traditional Chinese medicine Dispensary of Foshan Second People's Hospital,and appraised by Professor Xu from Science of Chinese Materia Medica College,Guangzhou University of Traditional Chinese Medicine.3H-PAF was supplied by Amersham Co.,Ltd.(specific activity:6.475 TBq/mmol;batch number:200402);PAF standard by Biomol Co.,Ltd.(batch number:P1318V).METHODS:The experiments were carried out in the Laboratory of Nuclear Medicine,Guangzhou University of Traditional Chinese Medicine from September to December 2004.①Injections of BHD,prescriptions for activating blood circulation and invigorating qi were prepared by the decoction and alcohol sedimentation technique.Rabbit common carotid artery blood(40 mL)was drawn via intubation to prepare platelet suspension of (0.8-1.0) ×1010L-1.②Determination of 3H-PAF and washed PAFR binding:The general combination tube(T) contained washed platelet-rich plasma(WPRP)380μL +3H-PAF(0.35 nmol/L)10μL+distilled water 5μL:The nonspecific binding tube(P)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+cold PAF(1μmol/L)5μL:The sample tube(Y)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+experimental medicine(injection of BHD,prescriptions for activating blood circulation or invigorating qi)5μL.The test was conducted for three times for each sample in the same way as mentioned above.The samples were shaken on the oscillator for 30 s,then bathed at 25℃ for 40 minutes,and the reaction was terminated with cold Tris buffer containing 0.1% BSA.multichannel cell detachment separator was used for vacuum suction to filter the separated free 3H-PAF,and the filter paper was washed with cold Tris buffer for four times,then dried in the baking oven(80℃)for 1 hour,and placed in xylol liquid scintillator,and the radioactivity was determined automatically by the liquid scintillation detector.The mean of the three parallel tubes was calculated.The specific binging inhibition rate was calculated:SBIR=[(T-Y)/(T-P)]×100%].③Univariate analysis of variance was conducted.And for comparison of each paired groups,the q test was adopted.MAIN OUTCOME MEASURES:Effect of BHD whole prescription,prescriptions for activating blood circulation and invigorating qi on the specific binding inhibition rate of 3H-PAF and PAFR.RESULTS: BHD,prescriptions for activating blood circulation and invigorating qi were all able to inhibit the specific binding of 3H-PAF to PAFR,the specific blinding inhibition rates were(45.90±7.50)%.(97.90±1.84)% and(26.75±2.48)%,respectively,and there were significant differences between every two groups(P＜0.01).CONCLUSION:Single Astragalus mongholicus(120 g)can inhibit the specific blinding of PAFR in the platelet of the rabbit with 3H-PAF,but the combination of Astragalus mongholicus with the drugs for activating blood circulation in BHD can significantly decrease the inhibiting action of the latter on PAFR activity of the platelet,reflecting the combined mechanism of "removing blood stasis without injuring the vital qi"in BHD.展开更多
Purpose: To assess platelet activity in HIV-patients with and without antiretroviral therapy (ART), analysing the influence of the presence or absence of ABC in the regimen. Methods: Observational, pilot study, includ...Purpose: To assess platelet activity in HIV-patients with and without antiretroviral therapy (ART), analysing the influence of the presence or absence of ABC in the regimen. Methods: Observational, pilot study, including 30 asymptomatic HIV-patients: 20 on ART for at least 24 weeks and with undetectable HIV viral load - 10 on ABC, 10 on tenofovir (TDF) - and 10 na?ve patients, and a control group of 10 HIV-negative subjects. No subject was receiving drugs with antiagregant activity. Platelet activity was assessed by measuring time-dependent platelet aggregometry (electrical impedance on fasting whole blood), induced by ADP (1.25, 2.5 μM), collagen (0.5, 1 μg/mL), arachidonic acid (100, 200 μM), and U46619 (receptor agonist of the tromboxano A2) (1.25, 2.5 μM). Statistic program: SPSS, 16.0. Results: Demographic, anthropometric data, and cardiovascular risk factors were similar in all groups, but older age and longer time of HIV infection in the ABC group (50.4 vs 36.1, 34.2 and 42.7 years, respectively;p < 0.05, and 140.3 vs 88.1 and 48.3 months in the two other groups of HIV patients;p < 0.05). Mean CD4 cells count was 564/mm3. Platelet aggregation with exposure to U46619 was higher in the ABC compared with the TDF group (11.1 vs 4.4%;p = 0.007), na?ve patients (11.1 vs 5.7%;p = 0.014), and the HIV-negative group (11.1 vs 6.5%;p = 0.04). These differences remained significant when controlled for age and time of HIV infection. Conclusions: ABC increases platelet aggregability possibly in relation with the receptor of tromboxano. Wider studies are needed to confirm this hypothesis.展开更多
OBJECTIVE:To investigate whether cancer stem cells(CSCs) more efficiently activating platelets and evading immune surveillance than non-CSCs thus promoting metastasis.METHODS:We enriched and identified sphereforming c...OBJECTIVE:To investigate whether cancer stem cells(CSCs) more efficiently activating platelets and evading immune surveillance than non-CSCs thus promoting metastasis.METHODS:We enriched and identified sphereforming cells(SFCs) and coincubated washed platelets with several platelet activators including collagen,4T1 and SFCs.Platelet-coating tumor cells,platelet activation and TGF-β1 release were analyzed.Then natural kell cells(NK) were incubated with supernatants of different activated platelet samples what we called sample release(SR).The degranulation assay and NKG2 D expression on NK cells were conducted by flow cytometry.Finally tissue factor(TF) expression of SFCs or 4T1 were evaluated by western blot.RESULTS:Breast cancer cell line 4T1 could form spheres in serum-free medium at low adherence.Sphere-forming cells expressed high levels of the CD24-/lowCD44 + stem cell phenotype.Both sphere-forming cells or 4T1 were coated with abundant platelets while sphere-forming cells induced significantly higher expression of platelet activating receptor CD62 p than 4T1 did(P < 0.01).And sphere-forming cells induced platelets to produce more TGF-β1 than 4T1 did(P < 0.01).Furthermore,sample releases induced by sphere-forming cells caused more vigorous inhibition of NK cells antitumor reactivity(P < 0.05) and reduced NKG2 D expression(P < 0.01).The final results showed that sphere-forming cells expressed higher levels of TF than 4T1(P < 0.05).CONCLUSION:Our findings indicate that CSCs could efficiently activate platelets,induce platelets to secrete more TGF-β1,decrease NKG2 D expression and inhibit antitumor activity of NK cell,compared with 4T1.And higher levels of TF expression of CSCs may account for this correlation of CSCs and platelets.展开更多
Tumor metastasis is responsible for most mortality in cancer patients, and remains a challenge in clinical cancer treatment. Platelets can be recruited and activated by tumor cells, then adhere to circulating tumor ce...Tumor metastasis is responsible for most mortality in cancer patients, and remains a challenge in clinical cancer treatment. Platelets can be recruited and activated by tumor cells, then adhere to circulating tumor cells(CTCs) and assist tumor cells extravasate in distant organs. Therefore, nanoparticles specially hitchhiking on activated platelets are considered to have excellent targeting ability for primary tumor, CTCs and metastasis in distant organs. However, the activated tumor-homing platelets will release transforming growth factor-β(TGF-β), which promotes tumor metastasis and forms immunosuppressive microenvironment. Therefore, a multitalent strategy is needed to balance the accurate tumor tracking and alleviate the immunosuppressive signals. In this study, a fucoidan-functionalized micelle(FD/DOX) was constructed, which could efficiently adhere to activated platelets through P-selectin.Compared with the micelle without P-selectin targeting effect, FD/DOX had increased distribution in both tumor tissue and metastasis niche, and exhibited excellent anti-tumor and anti-metastasis efficacy on 4 T1 spontaneous metastasis model. In addition, due to the contribution of fucoidan, FD/DOXtreatment was confirmed to inhibit the expression of TGF-β, thereby stimulating anti-tumor immune response and reversing the immunosuppressive microenvironment. The fucoidan-functionalized activated platelets-hitchhiking micelle was promising for the metastatic cancer treatment.展开更多
In recent years,ventricular assist devices(VADs,also known as blood pumps)have gradually entered clinical practice and provide effective treatment for heart failure patients.However,adverse events related to mechanica...In recent years,ventricular assist devices(VADs,also known as blood pumps)have gradually entered clinical practice and provide effective treatment for heart failure patients.However,adverse events related to mechanical blood damage in patients receiving the VAD treatment,were often reported and have become a major concern during the development of VADs,limiting their clinical and economic benefits.Compared with bench and in-vivo testing,computational fluid dynamics(CFD)is flexible and inexpensive,offering the possibility to predict hemodynamics and mechanical blood damage in a purely numerical manner.Thus,CFD has become an important tool for the design,optimization and evaluation of blood pumps.The stringent requirements of blood pumps require high fidelity simulations.Thus,this article reviews the current state of the art in high-fidelity methodologies for simulating blood flow and blood damage for the development and evaluation of rotary blood pumps;design and optimization methods of rotatory blood pumps using CFD,as well as future challenges.展开更多
Reduced bioavailability of nitric oxide along with an increase in inflammatory cytokines,reactive oxygen species,cell adhesion molecules,and platelet hyperactivity are underlying causes of endothelial dysfunction.Bioa...Reduced bioavailability of nitric oxide along with an increase in inflammatory cytokines,reactive oxygen species,cell adhesion molecules,and platelet hyperactivity are underlying causes of endothelial dysfunction.Bioactive peptides derived from various plants have been shown to attenuate biomarkers of endothelial dysfunction.The objective of this study was to determine the effects of hemp seed protein hydrolysates(HSPH)in modulating biomarkers of endothelial dysfunction using an in vitro cell culture model and an ex vivo platelet activity assay.HUVEC cells were treated with HSPH from commercial variant X and ECO-commercial variant Y,followed by hydrogen peroxide to simulate oxidative stress and inflammation.Biomarkers of endothelial dysfunction were then measured to determine the protective properties of HSPH.Blood samples collected from healthy volunteers were used to determine the effect of the HSPH on ADP and hydrogen peroxide-induced platelet activity,using flow cytometry.The HSPH reduced the production of reactive oxygen species,inflammatory cytokines IL-8,IL-12p70 and IL-1β,and adhesion molecule vascular cell adhesion molecule 1.Additionally,treatment with HSPH also reduced platelet activation marker CD62P ex vivo.The results obtained from this study suggest HSPH alleviates biomarkers of endothelial dysfunction by reducing oxidative stress,inflammation and platelet activation.展开更多
文摘Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, we investigated the effect of arsenic on agonist-induced vasorelaxation and vasoconstriction using the isolated rat aortic rings in in vitro organ bath system. Treatment with inorganic arsenite (AsⅢ) inhibited acetylcholine-induced relaxation of aortic rings by inhibiting production of nitric oxide in endothelium.
文摘In 1995,Huang et al.reported that rhodocytin,asnake toxin purified from callosdasma rhodostoma venomstimulates platelet aggregation.Ten years later,Suzuki-Inoue et al.identified C-type lectin-like receptor 2(CLEC-2)on platelets as the rhodocytin receptor.Thereafter,several studies have showed that platelet CLEC-2 isinvolved in lymphatic/blood vessel separation,tumormetastasis and thrombus formation.
基金grants from Scientific Planning Program of Guangdong Province, No. 2004B36001009Scientific Research Funds of Guangdong Bureau of Traditional Chinese Medicine, No. 30002+1 种基金 Scientific Development Special Planning Funds of Foshan City, No. 200124Medical Scientific Research Program of Foshan City, No. 2000096
文摘BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final effect.In order to reveal the compatibility mechanism of BHD's prescription in treating and preventing ischemic cerebrovascular disease,we needed explore the effect and relation of ingredients in the prescription.OBJECTIVE:To observe the effect of Buyang Huanwu decoction(BHD)and Astragalus mongholicus on the activity of platelet activating factor receptor(PAFR)in the platelet of rabbits in vitro,and investigate the mechanism of Astragalus mongholicus.DESIGN:A decomposed recipes study.SETTING:Guangzhou University of Traditional Chinese Medicine.MATERIALS:Five New Zealand rabbits,weighing 2-3 kg,both sexes,were used.BHD was composed of Sheng Huang Qi 120 g,Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g,Hong Hua 3 g.The prescription for activating blood circulation consisted of Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g and Hong Hua 3 g.The prescription for invigorating qi consisted of 120 g Sheng Huang Qi.The prepared herbal pieces were purchased from the traditional Chinese medicine Dispensary of Foshan Second People's Hospital,and appraised by Professor Xu from Science of Chinese Materia Medica College,Guangzhou University of Traditional Chinese Medicine.3H-PAF was supplied by Amersham Co.,Ltd.(specific activity:6.475 TBq/mmol;batch number:200402);PAF standard by Biomol Co.,Ltd.(batch number:P1318V).METHODS:The experiments were carried out in the Laboratory of Nuclear Medicine,Guangzhou University of Traditional Chinese Medicine from September to December 2004.①Injections of BHD,prescriptions for activating blood circulation and invigorating qi were prepared by the decoction and alcohol sedimentation technique.Rabbit common carotid artery blood(40 mL)was drawn via intubation to prepare platelet suspension of (0.8-1.0) ×1010L-1.②Determination of 3H-PAF and washed PAFR binding:The general combination tube(T) contained washed platelet-rich plasma(WPRP)380μL +3H-PAF(0.35 nmol/L)10μL+distilled water 5μL:The nonspecific binding tube(P)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+cold PAF(1μmol/L)5μL:The sample tube(Y)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+experimental medicine(injection of BHD,prescriptions for activating blood circulation or invigorating qi)5μL.The test was conducted for three times for each sample in the same way as mentioned above.The samples were shaken on the oscillator for 30 s,then bathed at 25℃ for 40 minutes,and the reaction was terminated with cold Tris buffer containing 0.1% BSA.multichannel cell detachment separator was used for vacuum suction to filter the separated free 3H-PAF,and the filter paper was washed with cold Tris buffer for four times,then dried in the baking oven(80℃)for 1 hour,and placed in xylol liquid scintillator,and the radioactivity was determined automatically by the liquid scintillation detector.The mean of the three parallel tubes was calculated.The specific binging inhibition rate was calculated:SBIR=[(T-Y)/(T-P)]×100%].③Univariate analysis of variance was conducted.And for comparison of each paired groups,the q test was adopted.MAIN OUTCOME MEASURES:Effect of BHD whole prescription,prescriptions for activating blood circulation and invigorating qi on the specific binding inhibition rate of 3H-PAF and PAFR.RESULTS: BHD,prescriptions for activating blood circulation and invigorating qi were all able to inhibit the specific binding of 3H-PAF to PAFR,the specific blinding inhibition rates were(45.90±7.50)%.(97.90±1.84)% and(26.75±2.48)%,respectively,and there were significant differences between every two groups(P＜0.01).CONCLUSION:Single Astragalus mongholicus(120 g)can inhibit the specific blinding of PAFR in the platelet of the rabbit with 3H-PAF,but the combination of Astragalus mongholicus with the drugs for activating blood circulation in BHD can significantly decrease the inhibiting action of the latter on PAFR activity of the platelet,reflecting the combined mechanism of "removing blood stasis without injuring the vital qi"in BHD.
文摘Purpose: To assess platelet activity in HIV-patients with and without antiretroviral therapy (ART), analysing the influence of the presence or absence of ABC in the regimen. Methods: Observational, pilot study, including 30 asymptomatic HIV-patients: 20 on ART for at least 24 weeks and with undetectable HIV viral load - 10 on ABC, 10 on tenofovir (TDF) - and 10 na?ve patients, and a control group of 10 HIV-negative subjects. No subject was receiving drugs with antiagregant activity. Platelet activity was assessed by measuring time-dependent platelet aggregometry (electrical impedance on fasting whole blood), induced by ADP (1.25, 2.5 μM), collagen (0.5, 1 μg/mL), arachidonic acid (100, 200 μM), and U46619 (receptor agonist of the tromboxano A2) (1.25, 2.5 μM). Statistic program: SPSS, 16.0. Results: Demographic, anthropometric data, and cardiovascular risk factors were similar in all groups, but older age and longer time of HIV infection in the ABC group (50.4 vs 36.1, 34.2 and 42.7 years, respectively;p < 0.05, and 140.3 vs 88.1 and 48.3 months in the two other groups of HIV patients;p < 0.05). Mean CD4 cells count was 564/mm3. Platelet aggregation with exposure to U46619 was higher in the ABC compared with the TDF group (11.1 vs 4.4%;p = 0.007), na?ve patients (11.1 vs 5.7%;p = 0.014), and the HIV-negative group (11.1 vs 6.5%;p = 0.04). These differences remained significant when controlled for age and time of HIV infection. Conclusions: ABC increases platelet aggregability possibly in relation with the receptor of tromboxano. Wider studies are needed to confirm this hypothesis.
基金Supported by the National Natural Science Foundation of China(the Research of Traditional Chinese Medicien Regulating the Tumor Metastasis due to Cancer Stem Cell Related Expression of TF and TF/FⅦSignal Pathway Based on Traditional Chinese Mediceory of"Fu Du",No.81273947)the International S&T Cooperation Program Office of China(Herbal Medicine with Different Treatment Principles on Regulating the Biological Behaviour of Cancer Stem Cells and its Regulatory Factors,No.2013DFA32540)
文摘OBJECTIVE:To investigate whether cancer stem cells(CSCs) more efficiently activating platelets and evading immune surveillance than non-CSCs thus promoting metastasis.METHODS:We enriched and identified sphereforming cells(SFCs) and coincubated washed platelets with several platelet activators including collagen,4T1 and SFCs.Platelet-coating tumor cells,platelet activation and TGF-β1 release were analyzed.Then natural kell cells(NK) were incubated with supernatants of different activated platelet samples what we called sample release(SR).The degranulation assay and NKG2 D expression on NK cells were conducted by flow cytometry.Finally tissue factor(TF) expression of SFCs or 4T1 were evaluated by western blot.RESULTS:Breast cancer cell line 4T1 could form spheres in serum-free medium at low adherence.Sphere-forming cells expressed high levels of the CD24-/lowCD44 + stem cell phenotype.Both sphere-forming cells or 4T1 were coated with abundant platelets while sphere-forming cells induced significantly higher expression of platelet activating receptor CD62 p than 4T1 did(P < 0.01).And sphere-forming cells induced platelets to produce more TGF-β1 than 4T1 did(P < 0.01).Furthermore,sample releases induced by sphere-forming cells caused more vigorous inhibition of NK cells antitumor reactivity(P < 0.05) and reduced NKG2 D expression(P < 0.01).The final results showed that sphere-forming cells expressed higher levels of TF than 4T1(P < 0.05).CONCLUSION:Our findings indicate that CSCs could efficiently activate platelets,induce platelets to secrete more TGF-β1,decrease NKG2 D expression and inhibit antitumor activity of NK cell,compared with 4T1.And higher levels of TF expression of CSCs may account for this correlation of CSCs and platelets.
基金supported by National Natural Science Foundation of China(81974499 and 81690261)Sichuan Science and Technology Program(2018RZ0136)。
文摘Tumor metastasis is responsible for most mortality in cancer patients, and remains a challenge in clinical cancer treatment. Platelets can be recruited and activated by tumor cells, then adhere to circulating tumor cells(CTCs) and assist tumor cells extravasate in distant organs. Therefore, nanoparticles specially hitchhiking on activated platelets are considered to have excellent targeting ability for primary tumor, CTCs and metastasis in distant organs. However, the activated tumor-homing platelets will release transforming growth factor-β(TGF-β), which promotes tumor metastasis and forms immunosuppressive microenvironment. Therefore, a multitalent strategy is needed to balance the accurate tumor tracking and alleviate the immunosuppressive signals. In this study, a fucoidan-functionalized micelle(FD/DOX) was constructed, which could efficiently adhere to activated platelets through P-selectin.Compared with the micelle without P-selectin targeting effect, FD/DOX had increased distribution in both tumor tissue and metastasis niche, and exhibited excellent anti-tumor and anti-metastasis efficacy on 4 T1 spontaneous metastasis model. In addition, due to the contribution of fucoidan, FD/DOXtreatment was confirmed to inhibit the expression of TGF-β, thereby stimulating anti-tumor immune response and reversing the immunosuppressive microenvironment. The fucoidan-functionalized activated platelets-hitchhiking micelle was promising for the metastatic cancer treatment.
基金the National Natural Science Foundation of China(No.12072216)the Mobility Program of the Sino-German Center(No.M-0231).
文摘In recent years,ventricular assist devices(VADs,also known as blood pumps)have gradually entered clinical practice and provide effective treatment for heart failure patients.However,adverse events related to mechanical blood damage in patients receiving the VAD treatment,were often reported and have become a major concern during the development of VADs,limiting their clinical and economic benefits.Compared with bench and in-vivo testing,computational fluid dynamics(CFD)is flexible and inexpensive,offering the possibility to predict hemodynamics and mechanical blood damage in a purely numerical manner.Thus,CFD has become an important tool for the design,optimization and evaluation of blood pumps.The stringent requirements of blood pumps require high fidelity simulations.Thus,this article reviews the current state of the art in high-fidelity methodologies for simulating blood flow and blood damage for the development and evaluation of rotary blood pumps;design and optimization methods of rotatory blood pumps using CFD,as well as future challenges.
基金This project was funded by the Australian Research Council(ARC)Industrial Transformation Centre(ITTC)for Functional Grains(Project ID:IC140100027).
文摘Reduced bioavailability of nitric oxide along with an increase in inflammatory cytokines,reactive oxygen species,cell adhesion molecules,and platelet hyperactivity are underlying causes of endothelial dysfunction.Bioactive peptides derived from various plants have been shown to attenuate biomarkers of endothelial dysfunction.The objective of this study was to determine the effects of hemp seed protein hydrolysates(HSPH)in modulating biomarkers of endothelial dysfunction using an in vitro cell culture model and an ex vivo platelet activity assay.HUVEC cells were treated with HSPH from commercial variant X and ECO-commercial variant Y,followed by hydrogen peroxide to simulate oxidative stress and inflammation.Biomarkers of endothelial dysfunction were then measured to determine the protective properties of HSPH.Blood samples collected from healthy volunteers were used to determine the effect of the HSPH on ADP and hydrogen peroxide-induced platelet activity,using flow cytometry.The HSPH reduced the production of reactive oxygen species,inflammatory cytokines IL-8,IL-12p70 and IL-1β,and adhesion molecule vascular cell adhesion molecule 1.Additionally,treatment with HSPH also reduced platelet activation marker CD62P ex vivo.The results obtained from this study suggest HSPH alleviates biomarkers of endothelial dysfunction by reducing oxidative stress,inflammation and platelet activation.
