Fishery-independent surveys are often used for collecting high quality biological and ecological data to support fisheries management. A careful optimization of fishery-independent survey design is necessary to improv...Fishery-independent surveys are often used for collecting high quality biological and ecological data to support fisheries management. A careful optimization of fishery-independent survey design is necessary to improve the precision of survey estimates with cost-effective sampling efforts. We developed a simulation approach to evaluate and optimize the stratification scheme for a fishery-independent survey with multiple goals including estimation of abundance indices of individual species and species diversity indices. We compared the performances of the sampling designs with different stratification schemes for different goals over different months. Gains in precision of survey estimates from the stratification schemes were acquired compared to simple random sampling design for most indices. The stratification scheme with five strata performed the best. This study showed that the loss of precision of survey estimates due to the reduction of sampling efforts could be compensated by improved stratification schemes, which would reduce the cost and negative impacts of survey trawling on those species with low abundance in the fishery-independent survey. This study also suggests that optimization of a survey design differed with different survey objectives. A post-survey analysis can improve the stratification scheme of fishery-independent survey designs.展开更多
AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive...AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive and Liver Disease Department of our hospital were randomly selected. RT-PCR was used to detect the level of miR-106b in serum samples. Laboratory and imaging examinations were carried out to confirm the HCC diagnosis in patients who had a > 2-fold change in miR-106b levels. Ultrasound-guided biopsy was also used for HCC diagnosis when necessary. On this basis, the clinical data of these subjects, including history of hepatitis virus infection, obesity, long-term history of alcohol use and stage of HCC, were collected. Then, the impact of these factors on the level of miR1-06b in serum was analyzed. Furthermore, receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic efficacy of miR-106b for HCC. RESULTS A total of 35 subjects had abnormal serum miR-106b levels, of which 20 subjects were diagnosed with HCC. t-test revealed that the difference in serum miR-106b level in terms of sex, age, history of hepatitis virus infection, obesity and long-term history of alcohol use was not statistically significant. However, serum miR-106b levels in patients with advanced HCC (stage. /.) was higher than in patients with early HCC (stage./.), and the difference was statistically significant (P = 0.000). Moreover, the ROC curve revealed that the area under the curve value for miR-106b was 0.885, which shows that serum miR-106b level has a certain clinical value for HCC diagnosis. CONCLUSION The random sampling survey shows that serum miR-106b level is a valuable diagnostic marker for HCC. However, the diagnostic threshold value needs to be further researched.展开更多
Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implement...Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.展开更多
基金The Public Science and Technology Research Funds Projects of Ocean under contract No.201305030the Specialized Research Fund for the Doctoral Program of Higher Education under contract No.20120132130001
文摘Fishery-independent surveys are often used for collecting high quality biological and ecological data to support fisheries management. A careful optimization of fishery-independent survey design is necessary to improve the precision of survey estimates with cost-effective sampling efforts. We developed a simulation approach to evaluate and optimize the stratification scheme for a fishery-independent survey with multiple goals including estimation of abundance indices of individual species and species diversity indices. We compared the performances of the sampling designs with different stratification schemes for different goals over different months. Gains in precision of survey estimates from the stratification schemes were acquired compared to simple random sampling design for most indices. The stratification scheme with five strata performed the best. This study showed that the loss of precision of survey estimates due to the reduction of sampling efforts could be compensated by improved stratification schemes, which would reduce the cost and negative impacts of survey trawling on those species with low abundance in the fishery-independent survey. This study also suggests that optimization of a survey design differed with different survey objectives. A post-survey analysis can improve the stratification scheme of fishery-independent survey designs.
文摘AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive and Liver Disease Department of our hospital were randomly selected. RT-PCR was used to detect the level of miR-106b in serum samples. Laboratory and imaging examinations were carried out to confirm the HCC diagnosis in patients who had a > 2-fold change in miR-106b levels. Ultrasound-guided biopsy was also used for HCC diagnosis when necessary. On this basis, the clinical data of these subjects, including history of hepatitis virus infection, obesity, long-term history of alcohol use and stage of HCC, were collected. Then, the impact of these factors on the level of miR1-06b in serum was analyzed. Furthermore, receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic efficacy of miR-106b for HCC. RESULTS A total of 35 subjects had abnormal serum miR-106b levels, of which 20 subjects were diagnosed with HCC. t-test revealed that the difference in serum miR-106b level in terms of sex, age, history of hepatitis virus infection, obesity and long-term history of alcohol use was not statistically significant. However, serum miR-106b levels in patients with advanced HCC (stage. /.) was higher than in patients with early HCC (stage./.), and the difference was statistically significant (P = 0.000). Moreover, the ROC curve revealed that the area under the curve value for miR-106b was 0.885, which shows that serum miR-106b level has a certain clinical value for HCC diagnosis. CONCLUSION The random sampling survey shows that serum miR-106b level is a valuable diagnostic marker for HCC. However, the diagnostic threshold value needs to be further researched.
文摘Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.
