[Objectives]To determine the drying process of different functional rhubarbs.[Methods]The contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were determined by HPLC.The...[Objectives]To determine the drying process of different functional rhubarbs.[Methods]The contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were determined by HPLC.The results were analyzed by two-way analysis of variance.[Results]Under different drying conditions,the contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were significantly different.Taking the tannins content as the index,the optimum drying condition of the rhizome is 1 cm thick,dried at 60℃,the root is cut 1 cm thick,and dried at 30℃;the bianthrone content is used as an indicator,the rhizome is optimally dried.The condition is to cut 1 cm thick,dry at 60℃,the root is cut to 5 cm thick,and dried at 40℃;the free anthraquinones content is used as an index,and the optimum drying condition of the rhizome is 3 cm thick and dried at 50℃.The root is cut to a thickness of 3 cm and dried at 30℃.The combine anthraquinones content is used as an indicator.The optimum drying conditions for the rhizome are 5 cm thick,dried at 40℃,and the root is cut to 5 cm thick and dried at 70℃.[Conclusions]Different functional components of rhubarb have different directional processing methods.The drying process can reduce the drying temperature or increase the thickness of the slice,and the directional processing of the diarrhea-type rhubarb can be processed.The drying process can be carried out by increasing the drying temperature or reducing the thickness of the slice directed processing of heat-clearing and purging-fire rhubarb.展开更多
Rheum officinale,a member of the Polygonaceae family,is an important medicinal plant that is widely used in traditional Chinese medicine.Here,we report a 7.68-Gb chromosome-scale assembly of R.officinale with a contig...Rheum officinale,a member of the Polygonaceae family,is an important medicinal plant that is widely used in traditional Chinese medicine.Here,we report a 7.68-Gb chromosome-scale assembly of R.officinale with a contig N50 of 3.47 Mb,which was clustered into 44 chromosomes across four homologous groups.Comparative genomics analysis revealed that transposable elements have made a significant contribution to its genome evolution,gene copy number variation,and gene regulation and expression,particularly of genes involved in metabolite biosynthesis,stress resistance,and root development.We placed the recent autotetraploidization of R.officinale at~0.58 mya and analyzed the genomic features of its homol-ogous chromosomes.Although no dominant monoploid genomes were observed at the overall expression level,numerous allele-differentially-expressed genes were identified,mainly with different transposable element insertions in their regulatory regions,suggesting that they functionally diverged after polyploidization.Combining genomics,transcriptomics,and metabolomics,we explored the contributions of gene family amplification and tetraploidization to the abundant anthraquinone production of R.officinale,as well as gene expression patterns and differences in anthraquinone content among tissues.Our report of-fers unprecedented genomic resources for fundamental research on the autopolyploid herb R.officinale and guidance for polyploid breeding of herbs.展开更多
Objective： To observe the effect of Da Huang（Rheum Officinale） application at Shenque（CV 8） on constipation after operation for lumbar vertebral fracture.Methods： Seventy-four patients with constipation after op...Objective： To observe the effect of Da Huang（Rheum Officinale） application at Shenque（CV 8） on constipation after operation for lumbar vertebral fracture.Methods： Seventy-four patients with constipation after operation for lumbar vertebral fracture were divided into an observation group and a control group by the random number table, 37 cases in each group. The two groups both received ordinary treatment（including healthcare guidance, emotional care, diet arrangement, and defecation nursing）, while the observation group was additionally given Da Huang（Rheum Officinale） application at Shenque（CV 8） for 6 h each day. The therapeutic efficacy was evaluated after successive 3-day treatment. Results： After 3-day treatment, it took significantly less time for the observation group to conduct the first flatulence and defecation than for the control group（P〈0.05）; the therapeutic efficacy of the observation group was significantly higher than that of the control group（P〈0.01）. Conclusion： Da Huang（Rheum Officinale） application at Shenque（CV 8） is effective in treating constipation after lumbar vertebral fracture operation, and it＇s easy-to-operate and well accepted by patients, hence it＇s proper to promote this method in clinic.展开更多
基金National Natural Science Foundation of China(31570331,31170307)National Key Research and Development Program of China(2017YFC1700702)Project of Beijing University of Chinese Medicine(2019-JYB-XS-087).
文摘[Objectives]To determine the drying process of different functional rhubarbs.[Methods]The contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were determined by HPLC.The results were analyzed by two-way analysis of variance.[Results]Under different drying conditions,the contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were significantly different.Taking the tannins content as the index,the optimum drying condition of the rhizome is 1 cm thick,dried at 60℃,the root is cut 1 cm thick,and dried at 30℃;the bianthrone content is used as an indicator,the rhizome is optimally dried.The condition is to cut 1 cm thick,dry at 60℃,the root is cut to 5 cm thick,and dried at 40℃;the free anthraquinones content is used as an index,and the optimum drying condition of the rhizome is 3 cm thick and dried at 50℃.The root is cut to a thickness of 3 cm and dried at 30℃.The combine anthraquinones content is used as an indicator.The optimum drying conditions for the rhizome are 5 cm thick,dried at 40℃,and the root is cut to 5 cm thick and dried at 70℃.[Conclusions]Different functional components of rhubarb have different directional processing methods.The drying process can reduce the drying temperature or increase the thickness of the slice,and the directional processing of the diarrhea-type rhubarb can be processed.The drying process can be carried out by increasing the drying temperature or reducing the thickness of the slice directed processing of heat-clearing and purging-fire rhubarb.
基金supported by the Natural Science Foundation of Hebei Province (grant C2021201048)the National Natural Science Foundation of China (grant 32100500)the Young Elite Scientists Sponsorship Program by CAST grant YESS20210080.
文摘Rheum officinale,a member of the Polygonaceae family,is an important medicinal plant that is widely used in traditional Chinese medicine.Here,we report a 7.68-Gb chromosome-scale assembly of R.officinale with a contig N50 of 3.47 Mb,which was clustered into 44 chromosomes across four homologous groups.Comparative genomics analysis revealed that transposable elements have made a significant contribution to its genome evolution,gene copy number variation,and gene regulation and expression,particularly of genes involved in metabolite biosynthesis,stress resistance,and root development.We placed the recent autotetraploidization of R.officinale at~0.58 mya and analyzed the genomic features of its homol-ogous chromosomes.Although no dominant monoploid genomes were observed at the overall expression level,numerous allele-differentially-expressed genes were identified,mainly with different transposable element insertions in their regulatory regions,suggesting that they functionally diverged after polyploidization.Combining genomics,transcriptomics,and metabolomics,we explored the contributions of gene family amplification and tetraploidization to the abundant anthraquinone production of R.officinale,as well as gene expression patterns and differences in anthraquinone content among tissues.Our report of-fers unprecedented genomic resources for fundamental research on the autopolyploid herb R.officinale and guidance for polyploid breeding of herbs.
基金supported by Planning Project of Chinese Medicine Science of Zhejiang ProvinceNo.2012ZB034~~
文摘Objective： To observe the effect of Da Huang（Rheum Officinale） application at Shenque（CV 8） on constipation after operation for lumbar vertebral fracture.Methods： Seventy-four patients with constipation after operation for lumbar vertebral fracture were divided into an observation group and a control group by the random number table, 37 cases in each group. The two groups both received ordinary treatment（including healthcare guidance, emotional care, diet arrangement, and defecation nursing）, while the observation group was additionally given Da Huang（Rheum Officinale） application at Shenque（CV 8） for 6 h each day. The therapeutic efficacy was evaluated after successive 3-day treatment. Results： After 3-day treatment, it took significantly less time for the observation group to conduct the first flatulence and defecation than for the control group（P〈0.05）; the therapeutic efficacy of the observation group was significantly higher than that of the control group（P〈0.01）. Conclusion： Da Huang（Rheum Officinale） application at Shenque（CV 8） is effective in treating constipation after lumbar vertebral fracture operation, and it＇s easy-to-operate and well accepted by patients, hence it＇s proper to promote this method in clinic.
