A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. zalacca （salak palm）. The marker is 1579 bp long and has a GC content of 38.5 %. Its sequence co...A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. zalacca （salak palm）. The marker is 1579 bp long and has a GC content of 38.5 %. Its sequence contains 1 or 2 open reading frames, indicating the marker is probably a coding region. No highly similar sequences were found in a search of the GenBank database. Sexes were identified using the SCAR DNA marker for three kinds of seedlings grouped by the number of seeds per fruit （1, 2 or 3）. The sex ratio of female to male did not differ significantly from 1：1 for the three kinds of seedlings, implying that the number of seeds per fruit is not a reliable index to identify the sex of a seedling.展开更多
In this study, the weather conditions of the accident at Maroua-Salak airport on 02 August 2020 are analyzed. This C130 aircraft accident took place under bad weather conditions. This bad weather is diagnosed using th...In this study, the weather conditions of the accident at Maroua-Salak airport on 02 August 2020 are analyzed. This C130 aircraft accident took place under bad weather conditions. This bad weather is diagnosed using the models as CFS, GFS and GEFS that reanalyze outputs of the results. At the end of this re-analysis, the result obtained shows that the event which took place at the Maroua-Salak airport was predictable. However, the analysis of these results has made it possible to present the rainfall cumul between 05 and 10 mm for this day. In addition, a comparative study of the rainfall cumul obtained by the CFS, GFS and GEFS models and that estimated by the satellites has been carried out. These models can significantly improve the prevention of such disasters in the region.展开更多
以蛇皮果顶梢嫩叶为材料,比较了CTAB法、改良CTAB法、CTAB-free法和SDS法对基因组DNA的提取效果,并对蛇皮果RAPD反应体系进行优化。结果表明,CTAB法、改良CTAB法和CTAB-free法均能提取出蛇皮果基因组DNA,其中NaCl浓度为5 m ol/L的改良C...以蛇皮果顶梢嫩叶为材料,比较了CTAB法、改良CTAB法、CTAB-free法和SDS法对基因组DNA的提取效果,并对蛇皮果RAPD反应体系进行优化。结果表明,CTAB法、改良CTAB法和CTAB-free法均能提取出蛇皮果基因组DNA,其中NaCl浓度为5 m ol/L的改良CTAB法因可更有效地去除多糖及酚类物质,并能满足PCR扩增的要求而被认为是蛇皮果基因组DNA提取的最适方法;在15μL的PCR扩增体系中,Mg2+、dNTPs和Taq DNA聚合酶的最优浓度分别为1.4 mmol/L、0.12 mmol/L和0.12 U/μL;PC R反应程序的最适退火温度为39℃。展开更多
基金supported by the State Administration of Forestry of China(Grant Numbers 201204307-03,2007-4-11,and 2012-LY-052)the Research Institute of Tropical Forestry,Chinese Academy of Forestry(Grant Number RITFKYYW2010-02)
文摘A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. zalacca （salak palm）. The marker is 1579 bp long and has a GC content of 38.5 %. Its sequence contains 1 or 2 open reading frames, indicating the marker is probably a coding region. No highly similar sequences were found in a search of the GenBank database. Sexes were identified using the SCAR DNA marker for three kinds of seedlings grouped by the number of seeds per fruit （1, 2 or 3）. The sex ratio of female to male did not differ significantly from 1：1 for the three kinds of seedlings, implying that the number of seeds per fruit is not a reliable index to identify the sex of a seedling.
文摘In this study, the weather conditions of the accident at Maroua-Salak airport on 02 August 2020 are analyzed. This C130 aircraft accident took place under bad weather conditions. This bad weather is diagnosed using the models as CFS, GFS and GEFS that reanalyze outputs of the results. At the end of this re-analysis, the result obtained shows that the event which took place at the Maroua-Salak airport was predictable. However, the analysis of these results has made it possible to present the rainfall cumul between 05 and 10 mm for this day. In addition, a comparative study of the rainfall cumul obtained by the CFS, GFS and GEFS models and that estimated by the satellites has been carried out. These models can significantly improve the prevention of such disasters in the region.
