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Flavonol synthase gene MsFLS13 regulates saline-alkali stress tolerance in alfalfa 被引量:2
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作者 Lishuang Zhang Yugang Sun +7 位作者 Jinqiang Ji Weidi Zhao Weileng Guo Jiaqi Li Yan Bai Dan Wang Zhe Yan Changhong Guo 《The Crop Journal》 SCIE CSCD 2023年第4期1218-1229,共12页
Alfalfa(Medicago sativa L.) is one of the most extensively grown leguminous forage worldwide.Environmental saline-alkali stress significantly influences the growth,development,and yield of alfalfa,posing a threat to i... Alfalfa(Medicago sativa L.) is one of the most extensively grown leguminous forage worldwide.Environmental saline-alkali stress significantly influences the growth,development,and yield of alfalfa,posing a threat to its agricultural production.However,little is known about the potential mechanisms by which alfalfa responds to saline-alkali stress.Here,we investigated these mechanisms by cloning a saline-alkali-induced flavonol synthase gene(Ms FLS13) from alfalfa,which was previously reported to be significantly upregulated under saline-alkali stress,and examining its function in the saline-alkali response.Overexpression of Ms FLS13 in alfalfa promoted plant tolerance to saline-alkali stress by enhancing flavonol accumulation,antioxidant capacity,osmotic balance,and photosynthetic efficiency.Conversely,Ms FLS13 inhibition using RNA interference reduced flavonol synthase activity and inhibited hairy root growth under saline-alkali stress.Yeast one-hybrid and dual-luciferase reporter assays indicated that the R2R3-MYB Ms MYB12 transcription factor activates Ms FLS13 expression by binding to the MBS motif in the Ms FLS13 promoter.Further analysis revealed that abscisic acid mediates the salinealkali stress response partially by inducing Ms MYB12 and Ms FLS13 expression,which consequently increases flavonol levels and maintains antioxidant homeostasis in alfalfa.Collectively,our findings highlight the crucial role of Ms FLS13 in alfalfa in response to saline-alkali stress and provide a novel genetic resource for creating saline-alkali-resistant alfalfa through genetic engineering. 展开更多
关键词 ALFALFA saline-alkali Msfls13 Msmyb12 ANTIOXIDANT
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Osmotic Regulation of Betaine Content in Leymus chinensis Under Saline-alkali Stress and Cloning and Expression of Betaine Aldehyde Dehydrogenase(BADH) Gene 被引量:8
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作者 CUI Xi-yan WANG Yong GUO Ji-xun 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第2期204-209,共6页
The potted Leymus chinensis seedlings were treated with saline-alkali solution of six different(from Ⅰ to Ⅵ) concentrations. The results demonstrate that the betaine content and Betaine-aldehyde dehydrogenase(BAD... The potted Leymus chinensis seedlings were treated with saline-alkali solution of six different(from Ⅰ to Ⅵ) concentrations. The results demonstrate that the betaine content and Betaine-aldehyde dehydrogenase(BADH: EC 1.2.1.8) activities have a direct relation with increased stressing time in the same treatment; both exhibit a single peak with increasing the concentration of saline-alkali solution, and number V shows the highest value. The BADH gene of Leyrnus chinensis was cloned by RT-PCR and RACE technology and was designated as LcBADH. The cDNA sequence of LcBADH was 1774bp including the open reading frame(ORF) of 1521bp(coding 506 amino acids). The vector of prokaryotic expression was constructed by inserting the LcBADH gene fragmcnt into pET30a(+) and transformed into E. coli BL21(DE3). The result of SDS-PAGE shows that the idio-protein with a molecular mass of 56.78 kDa was effectively expressed in the recombinant bacteria induced by isopropyl fl-D-thiogalactoside(IPTG). 展开更多
关键词 Leymus chinensis saline-alkali stress BADH RACE Cloning and expression
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Effect of Saline-alkali Stress on Seed Germination and Seedling Growth of Oat 被引量:6
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作者 Wang Guijun Wang Lei +1 位作者 Meng Xuan Xu Zhenwen 《Plant Diseases and Pests》 CAS 2015年第2期26-29,41,共5页
Neutral black soil was mixed with strong saline-alkali soil in different weight ratios, then physiological indexes during seed germination and seedling growth in soil of different ratios and the corresponding leaching... Neutral black soil was mixed with strong saline-alkali soil in different weight ratios, then physiological indexes during seed germination and seedling growth in soil of different ratios and the corresponding leaching liquor treatments were observed to explore effect of saline-alkali stress on oat seed germination and seedling growth, and analyze tolerance of oat to saline-alkali soil. The results showed that germination ability of oat seeds reduced with the increasing saline-alkali stress, salt injury index increased and seedling growth was inhibited. Effect of different saline-alkali stresses on germination ability of oat seeds showed difference, and effect of leaching liquor treatment on oat seed germination was stronger than that of soil treatment. 展开更多
关键词 saline-alkali stress OAT Seed germination Growth index INHIBITION
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Synergistic effect of Si and K in improving the growth,ion distribution and partitioning of Lolium perenne L.under saline-alkali stress 被引量:1
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作者 FAN Yuan SHEN Wu-yan +1 位作者 Pino VANESSA CHENG Fang-qin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1660-1673,共14页
The application of Si or K has proven to be beneficial for the growth of plants under saline-alkali stress. However, the synergistic effect of Si and K in improving the growth, ion distribution, and partitioning in Lo... The application of Si or K has proven to be beneficial for the growth of plants under saline-alkali stress. However, the synergistic effect of Si and K in improving the growth, ion distribution, and partitioning in Lolium perenne L. under saline-alkali stress remains unclear. In this study, the growth characteristics and ion-selective absorption of ryegrass(Lolium perenne L.) exposed to different levels of saline-alkali stress were evaluated. The growth parameters of ryegrass were significantly improved when Si was applied by itself or coupled with K under low saline-alkali stress. Under a high saline-alkali level, only simultaneous application of Si and K could significantly improve the growth of ryegrass. When Si and K were applied together, the K^(+)/Na^(+) and Ca^(2+)/Na^(+) ratios in root, stem, and leaf of ryegrass were maximally improved as compared to the individual treatments and control. The K^(+) and Ca^(2+) concentrations in the vacuole, cell wall, and organelle of leaf were increased dramatically. This improvement was due to the ability of applied ions to compete with Na^(+), allowing the plant to maintain osmotic potential and leaf water content. The concentration of Na^(+) was significantly reduced when Si and K were applied and mainly concentrated in the soluble fraction and cell wall. The Si concentration in ryegrass increased markedly by the combined application of Si and K, and most of it was accumulated in the cell wall and soluble fraction, which could help in chlorophyll synthesis, reduce membrane injury, and increase water absorption under saline-alkali stress. This study emphasized the advantage of Si and/or K on the growth of plants under different saline-alkaline levels and provided a guide for the production of Si-K fertilizer and its application in saline-alkali soil. 展开更多
关键词 saline-alkali stress SI K ryegrass plants K+/Na+ COMPARTMENTATION
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Overexpression of vacuolar proton pump ATPase(V-H^+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa(Medicago sativa L.) 被引量:5
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作者 WANG Fa-wei WANG Chao +9 位作者 SUN Yao WANG Nan LI Xiao-wei DONG Yuan-yuan YAO Na LIU Xiu-ming CHEN Huan CHEN Xi-feng WANG Zhen-min LI Hai-yan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第10期2279-2289,共11页
The vacuolar proton pump ATPase(V-H^+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several ... The vacuolar proton pump ATPase(V-H^+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H^+-ATPase subunits B(Sc VHA-B, Gen Bank: JF826506), C(Sc VHA-C, Gen Bank: JF826507) and H(Sc VHA-H, Gen Bank: JF826508) were isolated from the halophyte Suaeda corniculata. The transcript levels of Sc VHA-B, Sc VHA-C and Sc VHA-H were increased by salt, drought and saline-alkali treatments. V-H^+-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H^+-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H^+-ATPase subunits B, C and H(Sc VHA-B, Sc VHA-C and Sc VHA-H) from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type(WT). More free proline, higher superoxide dismutase(SOD) activity and lower malondialdehyde(MDA) levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the Sc VHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of Sc VHA-B, Sc VHA-C and Sc VHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa. 展开更多
关键词 vacuolar proton pump salt tolerance saline-alkali tolerance alfalfa
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Effects of an ectomycorrhizal fungus on the growth and physiology of Pinus sylvestris var. mongolica seedlings subjected to saline-alkali stress 被引量:2
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作者 Dachuan Yin Saiyaremu Halifu +3 位作者 Ruiqing Song Jinyu Qi Xun Deng Jifeng Deng 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第3期781-788,共8页
This research investigates the mechanism of increased salinity tolerance of ectomycorrhizal fungiinoculated P. sylvestris var. mongolica to provide a theoretical basis for the application of the fungus in saline soils... This research investigates the mechanism of increased salinity tolerance of ectomycorrhizal fungiinoculated P. sylvestris var. mongolica to provide a theoretical basis for the application of the fungus in saline soils.Growth effects due to inoculation of seedlings with Suillus luteus(a symbiotic ectomycorrhizal fungus), were determined in four kinds of saline–alkali soils. Growth and physiological indicators, including photosynthetic characteristics, plant height, biomass, photosynthetic pigments,catalase(CAT) and superoxide dismutase(SOD) enzyme levels, and malondialdehyde(MDA), an organic marker for oxidative stress, and soluble protein levels were determined. Mycorrhizal colonization rate decreased with increasing saline–alkalinity and growth of inoculated seedlings was significantly enhanced. Biomass and chlorophyll contents also increased significantly. SOD and CAT activities were higher than in non-inoculated seedlings. However, MDA content decreased in inoculatedseedlings. Soluble protein content did not increase significantly. Inoculation with a symbiotic ectomycorrhizal fungus could enhance the saline–alkali tolerance of P. sylvestris var. mongolica. Growth and physiological performance of inoculated seedlings were significantly better than that of uninoculated seedlings. The results indicate that inoculated P. sylvestris var. mongolica seedlings may be useful in the improvement of saline–alkali lands. 展开更多
关键词 ECTOMYCORRHIZAL fungi Saline–alkali stress PINUS SYLVESTRIS var MONGOLICA Physiological and biochemical mechanisms
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Photoresponse Process and Model Comparison of Paeonia ludlowii under Saline-alkali Stress
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作者 Ge Qingsong Gao Yi +1 位作者 Wang Hang Fang Jiangping 《Meteorological and Environmental Research》 CAS 2019年第4期84-87,92,共5页
The research aimed to analyze changes in photosynthetic characteristics of Paeonia ludlowii under saline-alkali stress, and annual seedlings of P. ludlowii were taken as the materials. Photoresponse process of P. ludl... The research aimed to analyze changes in photosynthetic characteristics of Paeonia ludlowii under saline-alkali stress, and annual seedlings of P. ludlowii were taken as the materials. Photoresponse process of P. ludlowii leaves under saline-alkali stress was simulated, and different models were used to fit photoresponse curve. The results showed that P n of P. ludlowii leaves showed the trend of first rising and then declining with PAR increased under saline-alkali stress;both G s and T r showed a rising trend with PAR increased;C i showed the trend of first declining and then rising with PAR increased. Photoresponse curve fitted by modified rectangular hyperbolic model had the best effect, and it was the optimal fitting model. P. ludlowii could adapt to saline-alkali stress in lower concentration, showing that P. ludlowii could be introduced and cultivated in saline-alkali land at a lower level. 展开更多
关键词 P. ludlowii saline-alkali stress PHOTOSYNTHETIC PARAMETERS PHOTORESPONSE MODEL
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Variation Characteristics of Root Traits of Different Alfalfa Cultivars under Saline-Alkaline Stress and their Relationship with Soil Environmental Factors
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作者 Tian-Jiao Wei Guang Li +6 位作者 Yan-Ru Cui Jiao Xie Xing-Ai Gao Xing Teng Xin-Ying Zhao Fa-Chun Guan Zheng-Wei Liang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第1期29-43,共15页
Soil salinization is the main factor that threatens the growth and development of plants and limits the increase of yield.It is of great significance to study the key soil environmental factors affecting plant root tr... Soil salinization is the main factor that threatens the growth and development of plants and limits the increase of yield.It is of great significance to study the key soil environmental factors affecting plant root traits to reveal the adaptation strategies of plants to saline-alkaline-stressed soil environments.In this study,the root biomass,root morphological parameters and root mineral nutrient content of two alfalfa cultivars with different sensitivities to alkaline stress were analyzed with black soil as the control group and the mixed saline-alkaline soil with a ratio of 7:3 between black soil and saline-alkaline soil as the saline-alkaline treatment group.At the same time,the correlation analysis of soil salinity indexes,soil nutrient indexes and the activities of key enzymes involved in soil carbon,nitrogen and phosphorus cycles was carried out.The results showed that compared with the control group,the pH,EC,and urease(URE)of the soil surrounding the roots of two alfalfa cultivars were significantly increased,while soil total nitrogen(TN),total phosphorus(TP),organic carbon(SOC),andα-glucosidase activity(AGC)were significantly decreased under saline-alkaline stress.There was no significant difference in root biomass and root morphological parameters of saline-alkaline tolerant cultivar GN under saline-alkaline stress.The number of root tips(RT),root surface area(RS)and root volume(RV)of AG were reduced by 61.16%,44.54%,and 45.31%,respectively,compared with control group.The ratios of K^(+)/Na^(+),Ca^(2+)/Na^(+)and Mg^(2+)/Na^(+)of GN were significantly higher than those of AG(p<0.05).The root fresh weight(RFW)and dry weight(RDW),root length(RL),RV and RT of alfalfa were positively regulated by soil SOC and TN,but negatively regulated by soil pH,EC,and URE(p<0.01).Root Ca^(2+)/Na+ratio was significantly positively correlated with soil TN,TP and SOC(p<0.01).The absorption of Mg and Ca ions in roots is significantly negatively regulated by soilβ-glucosidase activity(BGC)and acid phosphatase activity(APC)(p<0.05).This study improved knowledge of the relationship between root traits and soil environmental factors and offered a theoretical framework for elucidating how plant roots adapt to saline-alkaline stressed soil environments. 展开更多
关键词 saline-alkaline stress ALFALFA root traits soil environmental factors correlation analysis
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Ferroptosis and endoplasmic reticulum stress in ischemic stroke 被引量:6
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作者 Yina Li Mingyang Li +4 位作者 Shi Feng Qingxue Xu Xu Zhang Xiaoxing Xiong Lijuan Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期611-618,共8页
Ferroptosis is a form of non-apoptotic programmed cell death,and its mechanisms mainly involve the accumulation of lipid peroxides,imbalance in the amino acid antioxidant system,and disordered iron metabolism.The prim... Ferroptosis is a form of non-apoptotic programmed cell death,and its mechanisms mainly involve the accumulation of lipid peroxides,imbalance in the amino acid antioxidant system,and disordered iron metabolism.The primary organelle responsible for coordinating external challenges and internal cell demands is the endoplasmic reticulum,and the progression of inflammatory diseases can trigger endoplasmic reticulum stress.Evidence has suggested that ferroptosis may share pathways or interact with endoplasmic reticulum stress in many diseases and plays a role in cell survival.Ferroptosis and endoplasmic reticulum stress may occur after ischemic stroke.However,there are few reports on the interactions of ferroptosis and endoplasmic reticulum stress with ischemic stroke.This review summarized the recent research on the relationships between ferroptosis and endoplasmic reticulum stress and ischemic stroke,aiming to provide a reference for developing treatments for ischemic stroke. 展开更多
关键词 cell death endoplasmic reticulum stress ferroptosis ischemic stroke lipid peroxidation
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Grain Yield,Biomass Accumulation,and Leaf Photosynthetic Characteristics of Rice under Combined Salinity-Drought Stress 被引量:1
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作者 WEI Huanhe GENG Xiaoyu +7 位作者 ZHANG Xiang ZHU Wang ZHANG Xubin CHEN Yinglong HUO Zhongyang ZHOU Guisheng MENG Tianyao DAI Qigen 《Rice science》 SCIE CSCD 2024年第1期118-128,I0023,共12页
Simultaneous stresses of salinity and drought often coincide during rice-growing seasons in saline lands,primarily due to insufficient water resources and inadequate irrigation facilities.Consequently,combined salinit... Simultaneous stresses of salinity and drought often coincide during rice-growing seasons in saline lands,primarily due to insufficient water resources and inadequate irrigation facilities.Consequently,combined salinity-drought stress poses a major threat to rice production.In this study,two salinity levels(NS,non-salinity;HS,high salinity)along with three drought treatments(CC,control condition;DJ,drought stress imposed at jointing;DH,drought stress imposed at heading)were performed to investigate their combined influences on leaf photosynthetic characteristics,biomass accumulation,and rice yield formation.Salinity,drought,and their combination led to a shortened growth period from heading to maturity,resulting in a reduced overall growth duration.Grain yield was reduced under both salinity and drought stress,with a more substantial reduction under the combined salinity-drought stress.The combined stress imposed at heading caused greater yield losses in rice compared with the stress imposed at jointing.Additionally,the combined salinity-drought stress induced greater decreases in shoot biomass accumulation from heading to maturity,as well as in shoot biomass and nonstructural carbohydrate(NSC)content in the stem at heading and maturity.However,it increased the harvest index and NSC remobilization reserve.Salinity and drought reduced the leaf area index and SPAD value of flag leaves and weakened the leaf photosynthetic characteristics as indicated by lower photosynthetic rates,transpiration rates,and stomatal conductance.These reductions were more pronounced under the combined stress.Salinity,drought,and especially their combination,decreased the activities of ascorbate peroxidase,catalase,and superoxide dismutase,while increasing the contents of malondialdehyde,hydrogen peroxide,and superoxide radical.Our results indicated a more significant yield loss in rice when subjected to combined salinity-drought stress.The individual and combined stresses of salinity and drought diminished antioxidant enzyme activities,inhibited leaf photosynthetic functions,accelerated leaf senescence,and subsequently lowered assimilate accumulation and grain yield. 展开更多
关键词 antioxidant defense system combined salinity-drought stress drought stress photosynthetic characteristics RICE salinity stress
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Piezo1 channel exaggerates ferroptosis of nucleus pulposus cells by mediating mechanical stress-induced iron influx 被引量:2
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作者 Ziqian Xiang Pengfei Zhang +15 位作者 Chunwang Jia Rongkun Xu Dingren Cao Zhaoning Xu Tingting Lu Jingwei Liu Xiaoxiong Wang Cheng Qiu Wenyang Fu Weiwei Li Lei Cheng Qiang Yang Shiqing Feng Lianlei Wang Yunpeng Zhao Xinyu Liu 《Bone Research》 SCIE CAS CSCD 2024年第2期334-348,共15页
To date,several molecules have been found to facilitate iron influx,while the types of iron influx channels remain to be elucidated.Here,Piezo1 channel was identified as a key iron transporter in response to mechanica... To date,several molecules have been found to facilitate iron influx,while the types of iron influx channels remain to be elucidated.Here,Piezo1 channel was identified as a key iron transporter in response to mechanical stress.Piezo1-mediated iron overload disturbed iron metabolism and exaggerated ferroptosis in nucleus pulposus cells(NPCs).Importantly,Piezo1-induced iron influx was independent of the transferrin receptor(TFRC),a well-recognized iron gatekeeper.Furthermore,pharmacological inactivation of Piezo1 profoundly reduced iron accumulation,alleviated mitochondrial ROS,and suppressed ferroptotic alterations in stimulation of mechanical stress.Moreover,conditional knockout of Piezo1(Col2a1-CreERT Piezo1^(flox/flox))attenuated the mechanical injury-induced intervertebral disc degeneration(IVDD).Notably,the protective effect of Piezo1 deficiency in IVDD was dampened in Piezo1/Gpx4 conditional double knockout(cDKO)mice(Col2a1-CreERT Piezo1^(flox/flox)/Gpx4^(flox/flox)).These findings suggest that Piezo1 is a potential determinant of iron influx,indicating that the Piezo1-iron-ferroptosis axis might shed light on the treatment of mechanical stress-induced diseases. 展开更多
关键词 stress OVERLOAD CHANNEL
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Impact of effective stress on permeability for carbonate fractured-vuggy rocks 被引量:1
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作者 Ke Sun Huiqing Liu +5 位作者 Juliana Y.Leung Jing Wang Yabin Feng Renjie Liu Zhijiang Kang Yun Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第3期942-960,共19页
To gain insight into the flow mechanisms and stress sensitivity for fractured-vuggy reservoirs,several core models with different structural characteristics were designed and fabricated to investigate the impact of ef... To gain insight into the flow mechanisms and stress sensitivity for fractured-vuggy reservoirs,several core models with different structural characteristics were designed and fabricated to investigate the impact of effective stress on permeability for carbonate fractured-vuggy rocks(CFVR).It shows that the permeability performance curves under different pore and confining pressures(i.e.altered stress conditions)for the fractured core models and the vuggy core models have similar change patterns.The ranges of permeability variation are significantly wider at high pore pressures,indicating that permeability reduction is the most significant during the early stage of development for fractured-vuggy reservoirs.Since each obtained effective stress coefficient for permeability(ESCP)varies with the changes in confining pressure and pore pressure,the effective stresses for permeability of four representative CFVR show obvious nonlinear characteristics,and the variation ranges of ESCP are all between 0 and 1.Meanwhile,a comprehensive ESCP mathematical model considering triple media,including matrix pores,fractures,and dissolved vugs,was proposed.It is proved theoretically that the ESCP of CFVR generally varies between 0 and 1.Additionally,the regression results showed that the power model ranked highest among the four empirical models mainly applied in stress sensitivity characterization,followed by the logarithmic model,exponential model,and binomial model.The concept of“permeability decline rate”was introduced to better evaluate the stress sensitivity performance for CFVR,in which the one-fracture rock is the strongest,followed by the fracture-vug rock and two-horizontalfracture rock;the through-hole rock is the weakest.In general,this study provides a theoretical basis to guide the design of development and adjustment programs for carbonate fractured-vuggy reservoirs. 展开更多
关键词 Effective stress PERMEABILITY Carbonate fractured-vuggy rocks Structure characteristics stress sensitivity
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Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple 被引量:1
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作者 Qinglong Dong Yi Tian +9 位作者 Xuemei Zhang Dingyue Duan He Zhang Kaiyu Yang Peng Jia Haoan Luan Suping Guo Guohui Qi Ke Mao Fengwang Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期629-640,共12页
Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drou... Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain unclear.Here,we functionally characterized the apple GroupⅢWRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple. 展开更多
关键词 APPLE MdWRKY115 Osmotic stress Drought stress Function analysis
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Brain region-specific roles of brain-derived neurotrophic factor in social stress-induced depressive-like behavior 被引量:1
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作者 Man Han Deyang Zeng +7 位作者 Wei Tan Xingxing Chen Shuyuan Bai Qiong Wu Yushan Chen Zhen Wei Yufei Mei Yan Zeng 《Neural Regeneration Research》 SCIE CAS 2025年第1期159-173,共15页
Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral response.Recent studies have shown that brain-derived neurotrophic factor expression in stressed mice ... Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral response.Recent studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region–specific,particularly involving the corticolimbic system,including the ventral tegmental area,nucleus accumbens,prefrontal cortex,amygdala,and hippocampus.Determining how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress psychopathology.In this review,we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of depression.We focused on associated molecular pathways and neural circuits,with special attention to the brain-derived neurotrophic factor–tropomyosin receptor kinase B signaling pathway and the ventral tegmental area–nucleus accumbens dopamine circuit.We determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature,severity,and duration of stress,especially in the above-mentioned brain regions of the corticolimbic system.Therefore,BDNF might be a biological indicator regulating stress-related processes in various brain regions. 展开更多
关键词 AMYGDALA chronic mild stress chronic social defeat stress corticolimbic system DEPRESSION HIPPOCAMPUS medial prefrontal cortex nucleus accumbens social stress models ventral tegmental area
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Adverse effects of early-life stress:focus on the rodent neuroendocrine system 被引量:3
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作者 Seung Hyun Lee Eui-Man Jung 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期336-341,共6页
Early-life stress is associated with a high prevalence of mental illnesses such as post-traumatic stress disorders,attention-deficit/hyperactivity disorder,schizophrenia,and anxiety or depressive behavior,which consti... Early-life stress is associated with a high prevalence of mental illnesses such as post-traumatic stress disorders,attention-deficit/hyperactivity disorder,schizophrenia,and anxiety or depressive behavior,which constitute major public health problems.In the early stages of brain development after birth,events such as synaptogenesis,neuron maturation,and glial differentiation occur in a highly orchestrated manner,and external stress can cause adverse long-term effects throughout life.Our body utilizes multifaceted mechanisms,including neuroendocrine and neurotransmitter signaling pathways,to appropriately process external stress.Newborn individuals first exposed to early-life stress deploy neurogenesis as a stress-defense mechanism;however,in adulthood,early-life stress induces apoptosis of mature neurons,activation of immune responses,and reduction of neurotrophic factors,leading to anxiety,depression,and cognitive and memory dysfunction.This process involves the hypothalamus-pituitary-adrenal axis and neurotransmitters secreted by the central nervous system,including norepinephrine,dopamine,and serotonin.The rodent early-life stress model is generally used to experimentally assess the effects of stress during neurodevelopment.This paper reviews the use of the early-life stress model and stress response mechanisms of the body and discusses the experimental results regarding how early-life stress mediates stress-related pathways at a high vulnerability of psychiatric disorder in adulthood. 展开更多
关键词 early-life stress hypothalamic-pituitary-adrenergic axis maternal separation mental illness neurodevelopmental disorder neuroendocrine system NEUROTRANSMITTER
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A semi-analytical model for coupled flow in stress-sensitive multi-scale shale reservoirs with fractal characteristics 被引量:2
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作者 Qian Zhang Wen-Dong Wang +4 位作者 Yu-Liang Su Wei Chen Zheng-Dong Lei Lei Li Yong-Mao Hao 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期327-342,共16页
A large number of nanopores and complex fracture structures in shale reservoirs results in multi-scale flow of oil. With the development of shale oil reservoirs, the permeability of multi-scale media undergoes changes... A large number of nanopores and complex fracture structures in shale reservoirs results in multi-scale flow of oil. With the development of shale oil reservoirs, the permeability of multi-scale media undergoes changes due to stress sensitivity, which plays a crucial role in controlling pressure propagation and oil flow. This paper proposes a multi-scale coupled flow mathematical model of matrix nanopores, induced fractures, and hydraulic fractures. In this model, the micro-scale effects of shale oil flow in fractal nanopores, fractal induced fracture network, and stress sensitivity of multi-scale media are considered. We solved the model iteratively using Pedrosa transform, semi-analytic Segmented Bessel function, Laplace transform. The results of this model exhibit good agreement with the numerical solution and field production data, confirming the high accuracy of the model. As well, the influence of stress sensitivity on permeability, pressure and production is analyzed. It is shown that the permeability and production decrease significantly when induced fractures are weakly supported. Closed induced fractures can inhibit interporosity flow in the stimulated reservoir volume (SRV). It has been shown in sensitivity analysis that hydraulic fractures are beneficial to early production, and induced fractures in SRV are beneficial to middle production. The model can characterize multi-scale flow characteristics of shale oil, providing theoretical guidance for rapid productivity evaluation. 展开更多
关键词 Multi-scale coupled flow stress sensitivity Shale oil Micro-scale effect Fractal theory
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Genome-Wide Exploration of the Grape GLR Gene Family and Differential Responses of VvGLR3.1 and VvGLR3.2 to Low Temperature and Salt Stress 被引量:1
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作者 Honghui Sun Ruichao Liu +6 位作者 Yueting Qi Hongsheng Gao Xueting Wang Ning Jiang Xiaotong Guo Hongxia Zhang Chunyan Yu 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第3期533-549,共17页
Grapes,one of the oldest tree species globally,are rich in vitamins.However,environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality.The glutamate receptor(GLR... Grapes,one of the oldest tree species globally,are rich in vitamins.However,environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality.The glutamate receptor(GLR)family,comprising highly conserved ligand-gated ion channels,regulates plant growth and development in response to stress.In this study,11 members of the VvGLR gene family in grapes were identified using whole-genome sequence analysis.Bioinformatic methods were employed to analyze the basic physical and chemical properties,phylogenetic trees,conserved domains,motifs,expression patterns,and evolutionary relationships.Phylogenetic and collinear analyses revealed that the VvGLRs were divided into three subgroups,showing the high conservation of the grape GLR family.These members exhibited 2 glutamate receptor binding regions(GABAb and GluR)and 3-4 transmembrane regions(M1,M2,M3,and M4).Real-time quantitative PCR analysis demonstrated the sensitivity of all VvGLRs to low temperature and salt stress.Subsequent localization studies in Nicotiana tabacum verified that VvGLR3.1 and VvGLR3.2 proteins were located on the cell membrane and cell nucleus.Additionally,yeast transformation experiments confirmed the functionality of VvGLR3.1 and VvGLR3.2 in response to low temperature and salt stress.Thesefindings highlight the significant role of the GLR family,a highly conserved group of ion channels,in enhancing grape stress resistance.This study offers new insights into the grape GLR gene family,providing fundamental knowledge for further functional analysis and breeding of stress-resistant grapevines. 展开更多
关键词 Genome-wide identification glutamate receptor(GLR)family low temperature stress salt stress GRAPE
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Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress 被引量:1
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作者 Hao Liang Qiling Shi +8 位作者 Xing Li Peipei Gao Daling Feng Xiaomeng Zhang Yin Lu Jingsen Yan Shuxing Shen Jianjun Zhao Wei Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期461-472,共12页
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm... Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress. 展开更多
关键词 Chinese cabbage Salt stress Carbon metabolism PHOTOSYNTHESIS CHLOROPLAST
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Pre‑hatch thermal manipulation of embryos and post‑hatch baicalein supplementation mitigated heat stress in broiler chickens 被引量:1
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作者 Sadid Al Amaz Ajay Chaudhary +2 位作者 Prem Lal Mahato Rajesh Jha Birendra Mishra 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第3期1071-1085,共15页
Background High environmental temperatures induce heat stress in broiler chickens,affecting their health and pro-duction performance.Several dietary,managerial,and genetics strategies have been tested with some succes... Background High environmental temperatures induce heat stress in broiler chickens,affecting their health and pro-duction performance.Several dietary,managerial,and genetics strategies have been tested with some success in mitigating heat stress(HS)in broilers.Developing novel HS mitigation strategies for sustaining broiler production is critically needed.This study investigated the effects of pre-hatch thermal manipulation(TM)and post-hatch baica-lein supplementation on growth performance and health parameters in heat-stressed broilers.Results Six hundred fertile Cobb 500 eggs were incubated for 21 d.After candling on embryonic day(ED)10,238 eggs were thermally manipulated at 38.5℃ with 55%relative humidity(RH)from ED 12 to 18,then transferred to the hatcher(ED 19 to 21,standard temperature)and 236 eggs were incubated at a controlled temperature(37.5℃)till hatch.After hatch,180-day-old chicks from both groups were raised in 36 pens(n=10 birds/pen,6 replicates per treatment).The treatments were:1)Control,2)TM,3)control heat stress(CHS),4)thermal manipulation heat stress(TMHS),5)control heat stress supplement(CHSS),and 6)thermal manipulation heat stress supplement(TMHSS).All birds were raised under the standard environment for 21 d,followed by chronic heat stress from d 22 to 35(32–33℃ for 8 h)in the CHS,TMHS,CHSS,and TMHSS groups.A thermoneutral(22–24℃)environment was maintained in the Control and TM groups.RH was constant(50%±5%)throughout the trial.All the data were analyzed using one-way ANOVA in R and GraphPad software at P<0.05 and are presented as mean±SEM.Heat stress significantly decreased(P<0.05)the final body weight and ADG in CHS and TMHS groups compared to the other groups.Embryonic TM significantly increased(P<0.05)the expression of heat shock protein-related genes(HSP70,HSP90,and HSPH1)and antioxidant-related genes(GPX1 and TXN).TMHS birds showed a significant increment(P<0.05)in total cecal volatile fatty acid(VFA)concentration compared to the CHS birds.The cecal microbial analysis showed significant enrichment(P<0.05)in alpha and beta diversity and Coprococcus in the TMHSS group.Conclusions Pre-hatch TM and post-hatch baicalein supplementation in heat-stressed birds mitigate the detrimental effects of heat stress on chickens’growth performance,upregulate favorable gene expression,increase VFA produc-tion,and promote gut health by increasing beneficial microbial communities. 展开更多
关键词 BAICALEIN Growth performance Gut microbiota Heat stress Thermal manipulation
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Effect of icosahedral phase formation on the stress corrosion cracking(SCC)behaviors of the as-cast Mg-8%Li(in wt.%)based alloys 被引量:1
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作者 Shuo Wang Daokui Xu +2 位作者 Dongliang Wang Zhiqiang Zhang Baojie Wang 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2024年第1期225-236,共12页
Through exploring the stress corrosion cracking(SCC)behaviors of the as-cast Mg-8%Li and Mg-8%Li-6%Zn-1.2%Y alloys in a 0.1 M NaCl solution,it revealed that the SCC susceptibility index(I_(SCC))of the Mg-8%Li alloy wa... Through exploring the stress corrosion cracking(SCC)behaviors of the as-cast Mg-8%Li and Mg-8%Li-6%Zn-1.2%Y alloys in a 0.1 M NaCl solution,it revealed that the SCC susceptibility index(I_(SCC))of the Mg-8%Li alloy was 47%,whilst the I_(SCC)of the Mg-8%Li-6%Zn-1.2%Y alloy was 68%.Surface,cross-sectional and fractography observations indicated that for the Mg-8%Li alloy,theα-Mg/β-Li interfaces acted as the preferential crack initiation sites and propagation paths during the SCC process.With regard to the Mg-8%Li-6%Zn-1.2%Y alloy,the crack initiation sites included the I-phase and the interfaces of I-phase/β-Li andα-Mg/β-Li,and the preferential propagation paths were the I-phase/β-Li andα-Mg/β-Li interfaces.Moreover,the SCC of the two alloys was concerned with hydrogen embrittlement(HE)mechanism. 展开更多
关键词 Magnesium-lithium alloy stress corrosion cracking I-phase Fracture analysis
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