AIM: To investigate the expression of selenoprotein P mRNA (SePmRNA) in tissues of normal liver, liver cirrhosis and hepatocellular carcinoma (HCC), and its relationship with HCC occurrence and development. METHODS: T...AIM: To investigate the expression of selenoprotein P mRNA (SePmRNA) in tissues of normal liver, liver cirrhosis and hepatocellular carcinoma (HCC), and its relationship with HCC occurrence and development. METHODS: The expression of SePmRNA in tissues of normal liver, liver cirrhosis and HCC were detected by in situ hybridization using a cDNA probe. RESULTS: The enzyme digesting products of PBluescript-H uman Selenoprotein P were evaluated by electrophoresis. The positive expression of SePmRNA was found in the tissues of normal liver, liver cirrhosis and HCC. The expression of SeP mRNA was found in hepatic interstitial substance, especially in endothelial cells and lymphocytes of vasculature. The positive rate of SePmRNA in normal liver tissue was 84.6% (11/13) and the positive signals appeared in the nucleus and cytoplasm, mostly in the nucleolus, and the staining granules were larger in the nucleolus and around the nucleus. The positive rate of SePmRNA in liver cirrhosis tissue was 45.0% (9/20) and the positive signals were mainly in the nucleolus and cytoplasm, being less around the nucleus and inner nucleus than that in normal liver tissue. The positive rate of SePmRNA in HCC tissue was 30.0% (9/30) and the positive signals were in the cytoplasm, but less in the nucleus. CONCLUSION: SePmRNA expression in the tissues of normal liver and HCC is significantly different (84.6% vs 30.0%, P = 0.003), suggesting that SeP might play a role in the occurrence and development of HCC.展开更多
目的研究血清硒蛋白P(SEPP)、摄食抑制因子-1(Nesfatin-1)、肿瘤坏死因子-α(TNF-α)水平与妊娠糖尿病患者胰岛素抵抗的关系。方法回顾性选取2018年1月至2021年6月邢台医学高等专科学校第二附属医院收治的120例妊娠糖尿病患者为观察组,...目的研究血清硒蛋白P(SEPP)、摄食抑制因子-1(Nesfatin-1)、肿瘤坏死因子-α(TNF-α)水平与妊娠糖尿病患者胰岛素抵抗的关系。方法回顾性选取2018年1月至2021年6月邢台医学高等专科学校第二附属医院收治的120例妊娠糖尿病患者为观察组,选择同期健康体检产妇120名为对照组。比较两组血糖指标[空腹血糖(FBG)、餐后30 min血糖(30 min PG)、餐后1 h血糖(1 h PG)、餐后2 h血糖(2 h PG)]及胰岛素分泌指标[空腹胰岛素(FINS)、餐后30 min胰岛素(30 min INS)、餐后1 h胰岛素(1 h INS)、餐后2 h胰岛素(2 h INS)],对比两组胰岛素抵抗相关指数[胰岛素抵抗指数(HOMA-IR)、胰岛素β细胞功能指数(HOMA-β)、早期胰岛素分泌功能指数(△I_(30)/△G_(30))];比较两组血清SEPP、Nesfatin-1、TNF-α水平;分析血清SEPP、Nesfatin-1、TNF-α水平与妊娠糖尿病患者胰岛素抵抗的相关性。结果观察组FBG、30 min PG、1 h PG、2 h PG水平均高于对照组,差异均有统计学意义(P<0.05)。观察组FINS、1 h INS、2 h INS水平高于对照组,差异均有统计学意义(P<0.05),30 min INS较对照组比较差异无统计学意义(P>0.05)。观察组HOMA-IR高于对照组,HOMA-β、△I_(30)/△G_(30)低于对照组,差异均有统计学意义(P<0.05)。观察组Nesfatin-1、TNF-α水平高于对照组,SEPP水平低于对照组,差异均有统计学意义(P<0.05)。经Pearson检验,SEPP与HOMA-β、△I_(30)/△G_(30)呈正相(P<0.05),与HOMA-IR呈负相关(P<0.05);Nesfatin-1、TNF-α与HOMA-IR呈正相关(P<0.05),与HOMA-β、△I_(30)/△G_(30)呈负相关(P<0.05)。结论妊娠糖尿病患者血清Nesfatin-1、TNF-α水平明显上升,SEPP水平明显降低,且与胰岛素抵抗存在一定相关性。展开更多
基金Supported by Science and Technology Investigation and Development Project of Shaanxi Province, No. 2002K10-G1
文摘AIM: To investigate the expression of selenoprotein P mRNA (SePmRNA) in tissues of normal liver, liver cirrhosis and hepatocellular carcinoma (HCC), and its relationship with HCC occurrence and development. METHODS: The expression of SePmRNA in tissues of normal liver, liver cirrhosis and HCC were detected by in situ hybridization using a cDNA probe. RESULTS: The enzyme digesting products of PBluescript-H uman Selenoprotein P were evaluated by electrophoresis. The positive expression of SePmRNA was found in the tissues of normal liver, liver cirrhosis and HCC. The expression of SeP mRNA was found in hepatic interstitial substance, especially in endothelial cells and lymphocytes of vasculature. The positive rate of SePmRNA in normal liver tissue was 84.6% (11/13) and the positive signals appeared in the nucleus and cytoplasm, mostly in the nucleolus, and the staining granules were larger in the nucleolus and around the nucleus. The positive rate of SePmRNA in liver cirrhosis tissue was 45.0% (9/20) and the positive signals were mainly in the nucleolus and cytoplasm, being less around the nucleus and inner nucleus than that in normal liver tissue. The positive rate of SePmRNA in HCC tissue was 30.0% (9/30) and the positive signals were in the cytoplasm, but less in the nucleus. CONCLUSION: SePmRNA expression in the tissues of normal liver and HCC is significantly different (84.6% vs 30.0%, P = 0.003), suggesting that SeP might play a role in the occurrence and development of HCC.
文摘目的研究血清硒蛋白P(SEPP)、摄食抑制因子-1(Nesfatin-1)、肿瘤坏死因子-α(TNF-α)水平与妊娠糖尿病患者胰岛素抵抗的关系。方法回顾性选取2018年1月至2021年6月邢台医学高等专科学校第二附属医院收治的120例妊娠糖尿病患者为观察组,选择同期健康体检产妇120名为对照组。比较两组血糖指标[空腹血糖(FBG)、餐后30 min血糖(30 min PG)、餐后1 h血糖(1 h PG)、餐后2 h血糖(2 h PG)]及胰岛素分泌指标[空腹胰岛素(FINS)、餐后30 min胰岛素(30 min INS)、餐后1 h胰岛素(1 h INS)、餐后2 h胰岛素(2 h INS)],对比两组胰岛素抵抗相关指数[胰岛素抵抗指数(HOMA-IR)、胰岛素β细胞功能指数(HOMA-β)、早期胰岛素分泌功能指数(△I_(30)/△G_(30))];比较两组血清SEPP、Nesfatin-1、TNF-α水平;分析血清SEPP、Nesfatin-1、TNF-α水平与妊娠糖尿病患者胰岛素抵抗的相关性。结果观察组FBG、30 min PG、1 h PG、2 h PG水平均高于对照组,差异均有统计学意义(P<0.05)。观察组FINS、1 h INS、2 h INS水平高于对照组,差异均有统计学意义(P<0.05),30 min INS较对照组比较差异无统计学意义(P>0.05)。观察组HOMA-IR高于对照组,HOMA-β、△I_(30)/△G_(30)低于对照组,差异均有统计学意义(P<0.05)。观察组Nesfatin-1、TNF-α水平高于对照组,SEPP水平低于对照组,差异均有统计学意义(P<0.05)。经Pearson检验,SEPP与HOMA-β、△I_(30)/△G_(30)呈正相(P<0.05),与HOMA-IR呈负相关(P<0.05);Nesfatin-1、TNF-α与HOMA-IR呈正相关(P<0.05),与HOMA-β、△I_(30)/△G_(30)呈负相关(P<0.05)。结论妊娠糖尿病患者血清Nesfatin-1、TNF-α水平明显上升,SEPP水平明显降低,且与胰岛素抵抗存在一定相关性。
