Heavy metal ions in shellfish products are harmful to human health,and their removal with low nutrient loss remains challenging.Herein,a new type of mesoporous silica(SBA15),modified internally with ammonium pyrrolidi...Heavy metal ions in shellfish products are harmful to human health,and their removal with low nutrient loss remains challenging.Herein,a new type of mesoporous silica(SBA15),modified internally with ammonium pyrrolidine dithiocarbamate(APDC)and externally with alkyl-diol groups,which was named as Diol-APDC-SBA15,was successfully developed and characterized by powder X-ray diffraction patterns,nitrogen adsorption,and Fourier transform infrared spectroscopy.The solutions with lead,chromium,cadmium,and copper were used to investigate the adsorption capacity of Diol-APDC-SBA15.Diol-APDC-SBA15 was adopted to remove heavy metals from cooking liquids of clams(Ruditapes philippinarum),hydrolysate liquids of oysters(Ostrea gigas Thunberg),and polysaccharide solution from the cooking liquid of R.philippinarum.The efficiencies of removing heavy metal ions and the loss rates of proteins and polysaccharides were examined.The results showed that the adsorption capacities of Diol-APDCSBA15 for Pb,Cr,Cd,and Cu in standard heavy-metal solutions were 161.4,166.1,29.6,and 60.2mgg^(−1),respectively.The removal efficiency of Diol-APDC-SBA15 for Pb in the three shellfish processing liquids ranged from 60.5%to 99.6%.The Cr removal efficiency was above 99.9%in the oyster hydrolysate liquid.Meanwhile,the percentages of polysaccharide loss were 5.5%and 3.7%in the cooking liquid of clam and polysaccharide solution,respectively,and the protein loss was 1.2%in the oyster hydrolysate liquid.Therefore,the Diol-APDC-SBA15 material exhibits a great potential application in the removal of heavy metals from shellfish processing liquids with low losses of proteins and polysaccharides.展开更多
Bivalve farming plays a dominant role in mariculture in China.Paralytic shellfish toxins(PSTs)can be accumulated in bivalves and cause poisoning the consumers.A sensitive detection of PSTs can provide early warning to...Bivalve farming plays a dominant role in mariculture in China.Paralytic shellfish toxins(PSTs)can be accumulated in bivalves and cause poisoning the consumers.A sensitive detection of PSTs can provide early warning to decrease poisoning events in bivalve consuming.PSTs are traditionally examined using the whole soft-tissues.However,PSTs accumulation varies dramatically in different tissues of bivalves.Some tough tissues/organs(such as mantle),which account for a large proportion of the total soft body,exhibit a lower accumulation of PSTs and make the toxin extraction time-and reagent-consuming,potentially decreasing the accuracy and sensitivity of PSTs monitoring in bivalves.To develop a sensitive and cost-effective approach for PSTs examination in massively farmed bivalves,we fed three commercially important bivalves,Yesso scallop Patinopecten yessoensis,Pacific oyster Crassostrea gigas,and blue mussel Mytilus edulis with PSTs-producing dinoflagellate Alexandrium catenella,and detected PSTs concentration in different tissues.For all three bivalve species,the digestive gland accumulated much more PSTs than other tissues,and the digestive gland’s toxicity was significantly correlated with the PSTs toxicity of the whole soft-tissues,with r^(2)=0.94,0.92,and 0.94 for Yesso scallop,Pacific oyster,and blue mussel,respectively.When the toxicity of the whole soft-tissues reached 80μgSTXeq(100g)^(−1),the regulatory limit for commercial shellfish,the digestive gland’s toxicity reached 571.48,498.90,and 859.20μgSTXeq(100g)^(−1) in Yesso scallop,Pacific oyster,and blue mussel,respectively.Our results indicate that digestive gland can be used for the sensitive and cost-effective monitoring of PSTs in bivalves.展开更多
AIM: To assess current practice of United Kingdom cardiologists with respect to patients with reported shellfish/iodine allergy, and in particular the use of iodinated contrast for elective coronary angiography.Moreov...AIM: To assess current practice of United Kingdom cardiologists with respect to patients with reported shellfish/iodine allergy, and in particular the use of iodinated contrast for elective coronary angiography.Moreover we have reviewed the current evidence-base and guidelines available in this area.METHODS: A questionnaire survey was send to 500senior United Kingdom cardiologists(almost 50% cardiologists registered with British Cardiovascular Society)using email and first 100 responses used to analyze practise. We involved cardiologists performing coronary angiograms routinely both at secondary and tertiary centres. Three specific questions relating to allergy were asked:(1) History of shellfish/iodine allergy in pre-angiography assessment;(2) Treatments offeredfor shellfish/iodine allergy individuals; and(3) Any specific treatment protocol for shellfish/iodine allergy cases. We aimed to establish routine practice in United Kingdom for patients undergoing elective coronary angiography. We also performed comprehensive PubMed search for the available evidence of relationship between shellfish/iodine allergy and contrast media.RESULTS: A total of 100 responses were received, representing 20% of all United Kingdom cardiologists. Ninety-three replies were received from consultant cardiologists, 4 from non-consultant grades and 3 from cardiology specialist nurses. Amongst the respondents, 66% routinely asked about a previous history of shellfish/iodine allergy. Fifty-six percent would pre-treat these patients with steroids and anti-histamines. The other 44% do nothing, or do nonspecific testing based on their personal experience as following:(1) Skin test with 1 mL of subcutaneous contrast before intravenous contrast;(2) Test dose 2 mL contrast before coronary injection;(3) Close observation for shellfish allergy patients; and(4) Minimal evidence that the steroid and anti-histamine regime is effective but it makes us feel better.CONCLUSION: There is no evidence that allergy to shellfish alters the risk of reaction to intravenous contrast more than any other allergy and asking about such allergies in pre-angiogram assessment will not provide any additional information except propagating the myth.展开更多
Paralytic shellfish toxins(PSTs) are notorious neurotoxins that threaten public health and food safety worldwide.Although PST monitoring programs have recently been established throughout China, the profiles and varia...Paralytic shellfish toxins(PSTs) are notorious neurotoxins that threaten public health and food safety worldwide.Although PST monitoring programs have recently been established throughout China, the profiles and variation of PSTs in important commercial clams(e.g., Mactra veneriformis, Ruditapes philippinarum, and Meretrix meretrix) along the Jiangsu Province coastline remain largely unexplored. In this study, a validated hydrophilic interaction liquid chromatography–tandem mass spectrometry(HILIC-MS/MS) method was used to examine PST profiles and levels in 540 clam samples from natural production areas along Jiangsu Province coastline during2014–2016. Although the PST levels(≤6.38 μg saxitotoxin equivalents(eq)/kg) were consistently below European Union regulatory limits(≤800 μg saxitotoxin eq/kg) during this time period, saxitotoxin, decarbamoylsaxitotoxin,and gonyautoxins 1 and 4 were detected, and nearly 40% of the samples were saxitotoxin-positive. The PST levels also varied significantly by seasons, with peak values observed in May during 2014–2016. This is the first systematic report of PSTs in clams from Jiangsu Province, and additional research and protective measures are needed to ensure the safety of clams harvested in this area.展开更多
We measured the organic content and sinking velocities of biodeposits from two scallop species(Chlamys farreri,Patinopecten yessoensis) and abalone(Haliotis discus hannai) that were cultured on suspended long-lines.Me...We measured the organic content and sinking velocities of biodeposits from two scallop species(Chlamys farreri,Patinopecten yessoensis) and abalone(Haliotis discus hannai) that were cultured on suspended long-lines.Measurements were conducted every two months from April 2010 to February 2011.The shellfish were divided into three size groups(small,middle,and big sizes).At each sample point,we assessedbiodeposit organic content,average sinking velocity,the frequency distribution of sinkingvelocities,and the correlation between organic content and sinking velocity.The organic content of biodeposits varied significantly among months(P<0.05) and the pattern of change varied among species.Sinking velocities varied significantly,ranging from <0.5 cm/s to >1.9 cm/s.The sinking velocities of biodeposits from C.farreri and P.yessoensis were 0.5-1.5 cm/s and from H.discus hannai were <0.7 cm/s.The organic content was significantly negatively correlated to the sinking velocity of biodeposits in C.farreri(P<0.001) and P.yessoensis(P<0.05).展开更多
The process of ultrafiltration(UF)of natural seawater often encounters the problems of variation in water quality and coastal blooms.To validate the feasibility of UF in shellfish farms,this study compared the hydraul...The process of ultrafiltration(UF)of natural seawater often encounters the problems of variation in water quality and coastal blooms.To validate the feasibility of UF in shellfish farms,this study compared the hydraulic performance and pollutant removal efficiency of the UF process with those of the commonly used treatments that combine several filtration steps with ultraviolet(UV)disinfection.The comparison was conducted in the cases of natural seawater and a coastal bloom.Given that the UF process encountered the specific type of pollution,this study evaluated the filtration performance of the UF process and the retention of total suspended solids(TSS),bacteria,phytoplankton,and zooplankton.A real coastal bloom was considered in the case study of an experimental shellfish hatchery/nursery in France.The results show that both treatments were able to eliminate approximately 50%of TSS.However,in contrast with UV treatment combined with filtration,the UF process retained total amounts of phytoplankton,zooplankton,and bacteria in the bloom.Although the hydraulic performance of the UF process was impacted by the coastal bloom,the fouling was eliminated through chemical cleaning conducted at a frequency less than once per 12 h.Despite the severe pollution,this study confirmed the pollution resistance and treatment performance of the UF process,indicating that UF has the potential to enhance the biosecurity level.展开更多
Eelgrass species worldwide are valued for the ecosystem service they provide to estuarine and marine habitats. One species, Zostera japonica, however, has some negative impacts outside its native range and is consider...Eelgrass species worldwide are valued for the ecosystem service they provide to estuarine and marine habitats. One species, Zostera japonica, however, has some negative impacts outside its native range and is considered invasive. In Willapa Bay WA, USA, the nonnative eelgrass has expanded to the level where the shellfish industry is concerned about its potential impacts on its livelihood. Studies were conducted using paired plots, Z. japonica controlled with the herbicide imazamox vs. untreated controls, to assess the effects of Z. japonica on Manila clams (Ruditapes philippinarum) and Pacific oysters (Crassostrea gigas). Recruitment of new Manila clams was not affected by Z. japonica. The growth of young clams, total commercial clam harvests, clam quality and clam harvest efficiency, however, were greater on plots where Z. japonica was chemically controlled than where it was not treated. The response of oysters to Z. japonica control varied by site;there was no effect at one site, while the other sites had a 15% increase in shucked meat with Z. japonica control. The potential economic impact of a Z. japonica infestation of a shellfish bed was ~$47,000 ha-1 for Manila clams and $4000 ha-1 for oysters for each crop harvest cycle.展开更多
Objective To develop an ICR (female) mouse bioassay (MBA) for toxicity confirmation and evaluation of neurotoxins (brevetoxins)-contaminated shellfish. Methods Brevetoxins (BTX-B) as a causative agent of neurotoxic sh...Objective To develop an ICR (female) mouse bioassay (MBA) for toxicity confirmation and evaluation of neurotoxins (brevetoxins)-contaminated shellfish. Methods Brevetoxins (BTX-B) as a causative agent of neurotoxic shellfish poisoning (NSP) under different shellfish matrices were intraperitoneally injected at different doses into mice to study their toxic effects and to differentiate the range of lethal and sublethal dosages. Their sensitivity and specificity were analyzed with 2 competitive ELISA kits for quantitative determination of standard BTX-B and dihydroBTX-B under different shellfish matrix-diluent combinations. Detection rates of MBA and two antibody-based assays for BTX-B from field NSP-positive shellfish samples were compared. Results BTX-B could be detected in shellfish tissues at concentration of 50-400 μg/100 g under shellfish matrix-Tween-saline media, which were appropriate to identify toxic shellfish at or above the regulatory limit (80 μg/100 g shellfish tissues). The LD 50 identified was 455 g/kg for BTX-B under general shellfish matrices (excluding oyster matrices) dissolved in Tween-saline. The presence of shellfish matrices, of oyster matrices in particular, retarded the occurrence of death and toxicity presentation in mice. Two antibody-based assays, even in the presence of different shellfish matrix-diluent combinations, showed acceptable results in quantifying BTX-B and dihydroBTX-B well below the regulatory limit. Conclusion The two ELISA analyses agree favorably (correlation coefficient, r 0.96; Student's t-tests, P>0.05) with the developed bioassay.展开更多
In this article we explored the crystal structure of a few pearls and shellfish’s shell of fresh water and sea water, and found that pearls and nacreous layer (perhaps and prismatic layer) of shells are all the same:...In this article we explored the crystal structure of a few pearls and shellfish’s shell of fresh water and sea water, and found that pearls and nacreous layer (perhaps and prismatic layer) of shells are all the same: CaCO3, orthorhombic system, aragonite structure and the value of lattice spacings are almost identical. This shows that the pearls of fresh water and sea water are all the same in nature.展开更多
The dried shellfish products with rich nutrients and low-calorie content are favorite food in China,especially in coastal areas.However,the species of dried shellfish products in the market are usually unknown,as the ...The dried shellfish products with rich nutrients and low-calorie content are favorite food in China,especially in coastal areas.However,the species of dried shellfish products in the market are usually unknown,as the taxonomic features were removed during the production process.This study described the application of DNA barcoding technique to the identification of 100 dried shellfish(scallop,squid,octopus and cuttlefish)products in markets.Samples were authenticated by comparing mitochondrial cytochrome oxidase subunit I(COI)gene and 16S ribosomal RNA(16S rRNA)gene sequences with public reference taxonomic databases.The results showed that all the 100 products can be identified at species level.Sixty four scallop adductor products were processed using the bay scallop,Argopecten irradians,and one was from Portuguese oyster,Crassostrea angulata.All the 27 squid,2 cuttlefish and 6 octopus products were produced by the Jumbo flying squid,Dosidicus gigas.The neighbour-joining tree is in agreement with the results of DNA barcoding analysis.The 64 scallop samples formed one A.irradians cluster,leaving Sca65 clustered with the reference oyster sequence C.angulata(MH997922).All the 35 cephalopod(squid,octopus and cuttlefish)samples formed a D.gigas cluster.This investigation revealed a low variety of dried shellfish products sold on the market,and highlighted the high rate of mislabeling and species substitution.Our present work provides a practical method for tracing and authenticating shellfish products.展开更多
By using a farm's data in Yantai City and the theory of Cost-Volume-Profit analysis and the financial management methods,this paper construct a multi-factor analysis model for improving profit management using Exc...By using a farm's data in Yantai City and the theory of Cost-Volume-Profit analysis and the financial management methods,this paper construct a multi-factor analysis model for improving profit management using Excel 2007 in Shellfish farming projects and describes the procedures to construct a multi-factor analysis model.The model can quickly calculate the profit,improve the level of profit management,find out the breakeven point and enhance the decision-making efficiency of businesses etc.It is also a thought of the application to offer suggestions for government decisions and economic decisions for corporations as a simple analysis tool.While effort has been exerted to construct a four-variable model,some equally important variables may not be discussed sufficiently due to limitation of the paper's space and the authors'knowledge.All variables can be listed in EXCEL 2007 and can be associated in a logical way to manage the profit of shellfish farming projects more efficiently and more practically.展开更多
Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><sp...Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">)</span></span></span><span><span><span style="font-family:""> by canning via three treatments </span></span></span><span><span><span style="font-family:"">that </span></span></span><span><span><span style="font-family:"">are canned raw, canned smoked and canned cooked as a healthy product with high nutritional value. Results<span> </span>reported that edible meat of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> was free from Cu, Pb and Cd as a result of washing process. There was significantly (p < 0.05) higher in moisture content of canned raw compared with other tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">, and there was significantly reduction of protein content (p < 0.05) in tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked compared with the canned raw. Significant different (p < 0.05) in fat content </span></span></span><span><span><span style="font-family:"">w</span></span></span><span><span><span style="font-family:"">as canned raw < canned smoked < canned cooked. On the other side</span></span></span><span><span><span style="font-family:"">,</span></span></span><span><span><span style="font-family:""> the three treatments of canned shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:"">had significant differen</span></span></span><span><span><span style="font-family:"">ce</span></span></span><span><span><span style="font-family:""> (p < 0.05) in content of ash and carbohydrates content. Canned smoked of shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> showed the lowest value of pH. All treatments of canned shellfish<span> </span></span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> were rich in minerals where canned raw treatment was higher in Ca, Fe, Zn, Mg, and Mn. While canned smoked had minerals content lower than canned raw. Lightness (L*) had slightly differen</span></span></span><span><span><span style="font-family:"">ce</span></span></span><span><span><span style="font-family:""> between all treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia</span></i></span></span><span><span><i><span style="font-family:""> <span>undulate</span></span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">. Reduction on lightness and redness was noticed at the same time in all treatments of canned shellfish<span> </span></span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia</span></i></span></span><span><span><span style="font-family:""> <i>undulate</i></span></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">,</span></span></span><span><span><span style="font-family:""> canned raw had</span></span></span><span><span><span style="font-family:""> the </span></span></span><span><span><span style="font-family:"">highest value of lightness (L*), and redness (a*). Canned smoked had the highest value of hardness followed by canned raw then canned cook. Slightly significant difference (p < 0.05) was noticed between three treatments in sensory properties, data showed that all panelists prefer</span></span></span><span><span><span style="font-family:"">red</span></span></span><span><span><span style="font-family:""> canned cooked than raw canned and canned smoked, degree of canned cooked was excellent.展开更多
Our study investigated the effect of utiliz</span></span><span><span><span style="font-family:;" "="">ing</span></span></span><span><sp...Our study investigated the effect of utiliz</span></span><span><span><span style="font-family:;" "="">ing</span></span></span><span><span><span style="font-family:;" "=""> chicken</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">wings and dehydrated shellfish to produce chicken nuggets rich in protein, minerals and vitamins.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">The proximate composition, nutritional value, physical and sensorial properties were evaluated. The different</span></span></span><span><span><span style="font-family:;" "="">ly</span></span></span><span><span><span style="font-family:;" "=""> prepared chicken nuggets </span></span></span><span><span><span style="font-family:;" "="">were </span></span></span><span><span><span style="font-family:;" "="">supplemented with dehydrated shellfish CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">2%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% and CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6%. There was </span></span></span><span><span><span style="font-family:;" "="">a </span></span></span><span><span><span style="font-family:;" "="">significantly reduction </span></span></span><span><span><span style="font-family:;" "="">in </span></span></span><span><span><span style="font-family:;" "="">moisture and </span></span></span><span><span><span style="font-family:;" "="">an </span></span></span><span><span><span style="font-family:;" "="">increasing in protein content (<i>P</i> < 0.05) in all Chicken Nuggets (CN) in comparison to the control. Fat content was significantly higher in CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0% than CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">2%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% and CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6%. Ash content was increasing with added dehydrated shellfish, while </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">reduction in carbohydrates and total calories of the different chicken nuggets were noticed with </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">significant gradual rise in pH values, TBA values showed no significant difference (<i>P</i> < 0.05) between chicken nuggets. Results showed that using dehydrated shellfish in preparing chicken nuggets caused a marked rise in its content of Ca, Fe, Na, K, Zn, Mg and Mn levels, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0% had the lowest concentration of all minerals, and had lower value</span></span></span><span><span><span style="font-family:;" "="">s</span></span></span><span><span><span style="font-family:;" "=""> of vitamins A, E and D but CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6% had higher value</span></span></span><span><span><span style="font-family:;" "="">s</span></span></span><span><span><span style="font-family:;" "=""> of vitamins A, E and D. Control Nuggets (CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0%) had the lowest value of yellowness and highest value of lightness but CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6% had </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">highest value of yellowness and, </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">lowest value of lightness. </span></span></span><span><span><span style="font-family:;" "="">The </span></span></span><span><span><span style="font-family:;" "="">redness had </span></span></span><span><span><span style="font-family:;" "="">a </span></span></span><span><span><span style="font-family:;" "="">slightly reduction. All samples showed slight changes in</span></span></span><span><span><span style="font-family:;" "=""> hardness, cohesiveness, springiness, gumminess and chewiness were noticed. </span></span></span><span><span><span style="font-family:;" "="">An </span></span></span><span><span><span style="font-family:;" "="">increase in WHC, pick-up, and cooking loss of chicken nuggets with dehydrated shellfish was noticed. Panelists accepted all prepared chicken nuggets and the chicken nuggets CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% had </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">highest score compared with other chicken nuggets prepared.展开更多
Antibiotic resistant bacteria pathogens remain the leading cause of shellfish borne diseases and a major health threat to humans worldwide. The objectives of this study were to isolate, identify, and determine the ant...Antibiotic resistant bacteria pathogens remain the leading cause of shellfish borne diseases and a major health threat to humans worldwide. The objectives of this study were to isolate, identify, and determine the antibiotic resistance patterns of Gram-negative bacteria from shellfish. We analyzed a total of 540 shellfish (117 clams, 88 oysters, and 136 periwinkles) samples collected from different vendors at Iko and Douglas Creeks in Akwa Ibom State, South-South Nigeria. Conventional cultural techniques, morphological, biochemical characteristics, and PCR amplification were used to identify the bacterial isolates. Antibiotic susceptibility tests (Kirby-Bauer disk diffusion method) and ESBL phenotype (disk) of the isolates were performed. One hundred and thirty-five (135) Gram-negative bacteria comprising 5 genera and 14 species were detected at a prevalence of: <i>Alcaligenes faecalis</i> <i><b>TRB</b></i>-7 38 (28.2%), <i>Pseudomonas oryzihabitans strain <b>KCB</i>005</b> 16 (11.9%), <i>Paenalcaligenes retgerii strain <b>B</i>5</b> 12 (8.9%) <i>Pseudomonas aeruginosa <b>JB</i>2</b> 10 (7.4%), <i>Providencia stuartii <b>DMC</i>-28b</b> 9 (6.7%), <i>Alcaligenes species <b>TLT</i>151</b> 8 (5.9%), <i>Pseudomonas aeruginosa <b>CIFRI DTSB</i>1</b> 7 (5.2%), <i>Paenalcaligenes species <b>UN</i>24</b> 7 (5.2%), <i>Alcaligenes faecalis <b>BT</i>10</b> 7 (5.2%), <i>Vibrio species strain <b>PrVy</i>108</b> 6 (4.4%), <i>Pseudomonas xiamenensis <b>C</i>10-2</b> 5 (3.7%), <i>Providencia vemicola <b>Bu</i>15_38</b> 4 (2.9%), <i>Pseudomonas anguillisceptica</i> <b>4029</b> 3 (2.2%), and <i>Pseudomonas aeruginosa <b>N</i>15-01092</b> 3 (2.2%). All tested isolates showed various degrees of resistance to the thirteen antimicrobials evaluated. High levels of resistance (100%) to cefepime and imipenem were expressed by all isolates except the <i>Providencia</i> species. For the EBSL indicators, all isolates apart from <i>Alcaligenes</i> species were resistant (100%) to ceftriaxone. All <i>Vibrio</i> species were susceptible to norfloxacin, nalidixic acid, and ceftazidime. The identification of antibiotic resistant Gram-negative bacteria (GNARB) from shellfish in this study highlights the risk of disseminated multi-drug resistance—a serious public health concern.展开更多
Abdul Owor,a seasoned fisherman carries his day’s catch of lobsters along Mombasa beach and takes it to a nearby store room for sale.The store room is a lobster collection center belonging to a Chinese company that d...Abdul Owor,a seasoned fisherman carries his day’s catch of lobsters along Mombasa beach and takes it to a nearby store room for sale.The store room is a lobster collection center belonging to a Chinese company that deals in the export of live lobsters.Owor ’s catch is weighed and he is paid cash by the Kenyan attendant.Owor said that unlike in the past when he had to depend on Kenyan middlemen to market his lobsters.展开更多
Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a ma...Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction(RT-ddPCR). Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption.展开更多
Objective To study the transfer of paralytic shellfish toxins (PST) using four simulated marine food chains: dinoflagellate Alexandrium tamarense→Artemia Artemia salina→Mysid shrimp Neomysis awatschensis; A. tamare...Objective To study the transfer of paralytic shellfish toxins (PST) using four simulated marine food chains: dinoflagellate Alexandrium tamarense→Artemia Artemia salina→Mysid shrimp Neomysis awatschensis; A. tamarense→N. awatschensis; A. tamarense→A. salina→Perch Lateolabrax japonicus; and A. tamarense→L. japonicus. Methods The ingestion of A. tamarense, a producer of PST, by L. japonicus, N. awatschensis, and A. salina was first confirmed by microscopic observation of A. tamarense cells in the intestine samples of the three different organisms, and by the analysis of Chl.a levels in the samples. Toxin accumulation in L. japonicus and N. awatschensis directly from the feeding on A. tamarense or indirectly through the vector of A. salina was then studied. The toxicity of samples was measured using the AOAC mouse bioassay method, and the toxin content and profile of A. tamarense were analyzed by the HPLC method. Results Both A. salina and N. awatschensis could ingest A. tamarense cells. However, the ingestion capability of A. salina exceeded that of N. awatschensis. After the exposure to the culture of A. tamarense (2 000 cells·mL-1) for 70 minutes, the content of Chl.a in A. salina and N. awatschensis reached 0.87 and 0.024 μg·mg-1, respectively. Besides, A. tamarense cells existed in the intestines of L. japonicus, N. awatschensis and A. salina by microscopic observation. Therefore, the three organisms could ingest A. tamarense cells directly. A. salina could accumulate high content of PST, and the toxicity of A. salina in samples collected on days 1, 4, and 5 of the experiment was 2.18, 2.6, and 2.1 MU·g-1, respectively. All extracts from the samples could lead to death of tested mice within 7 minutes, and the toxin content in artemia sample collected on the 1st day was estimated to be 1.65×10-5 μg STX equal/individual. Toxin accumulation in L. japonicus and N. awatschensis directly from the feeding on A. tamarense or indirectly from the vector of A. salina was also studied. The mice injected with extracts from L. japonicus and N. awatschensis samples that accumulated PST either directly or indirectly showed PST intoxication symptoms, indicating that low levels of PST existed in these samples. Conclusion Paralytic shellfish toxins can be transferred to L. japonicus, N. awatschensis, and A. salina from A. tamarense directly or indirectly via the food chains.展开更多
基金supported by the National Key R&D Program of China(No.2018YFD0901004)the National Natural Science Foundation of China(No.31601538)+2 种基金the Key Science and Technology Program of Liaoning Province(No.2020JH1/10200001)the Fundamental Research Foundation of Education Department of Liaoning Province(No.JL202008)the Science&Technology Innovation Foundation of Dalian(No.2019J12SN61).
文摘Heavy metal ions in shellfish products are harmful to human health,and their removal with low nutrient loss remains challenging.Herein,a new type of mesoporous silica(SBA15),modified internally with ammonium pyrrolidine dithiocarbamate(APDC)and externally with alkyl-diol groups,which was named as Diol-APDC-SBA15,was successfully developed and characterized by powder X-ray diffraction patterns,nitrogen adsorption,and Fourier transform infrared spectroscopy.The solutions with lead,chromium,cadmium,and copper were used to investigate the adsorption capacity of Diol-APDC-SBA15.Diol-APDC-SBA15 was adopted to remove heavy metals from cooking liquids of clams(Ruditapes philippinarum),hydrolysate liquids of oysters(Ostrea gigas Thunberg),and polysaccharide solution from the cooking liquid of R.philippinarum.The efficiencies of removing heavy metal ions and the loss rates of proteins and polysaccharides were examined.The results showed that the adsorption capacities of Diol-APDCSBA15 for Pb,Cr,Cd,and Cu in standard heavy-metal solutions were 161.4,166.1,29.6,and 60.2mgg^(−1),respectively.The removal efficiency of Diol-APDC-SBA15 for Pb in the three shellfish processing liquids ranged from 60.5%to 99.6%.The Cr removal efficiency was above 99.9%in the oyster hydrolysate liquid.Meanwhile,the percentages of polysaccharide loss were 5.5%and 3.7%in the cooking liquid of clam and polysaccharide solution,respectively,and the protein loss was 1.2%in the oyster hydrolysate liquid.Therefore,the Diol-APDC-SBA15 material exhibits a great potential application in the removal of heavy metals from shellfish processing liquids with low losses of proteins and polysaccharides.
基金funded by the National Key R&D Project(No.2019YFC1605704)the Taishan Industry Leading Talent Project(No.LJNY201816)supported by Sanya Yazhou Bay Science and Technology City(No.SKJCKJ-2019KY01).
文摘Bivalve farming plays a dominant role in mariculture in China.Paralytic shellfish toxins(PSTs)can be accumulated in bivalves and cause poisoning the consumers.A sensitive detection of PSTs can provide early warning to decrease poisoning events in bivalve consuming.PSTs are traditionally examined using the whole soft-tissues.However,PSTs accumulation varies dramatically in different tissues of bivalves.Some tough tissues/organs(such as mantle),which account for a large proportion of the total soft body,exhibit a lower accumulation of PSTs and make the toxin extraction time-and reagent-consuming,potentially decreasing the accuracy and sensitivity of PSTs monitoring in bivalves.To develop a sensitive and cost-effective approach for PSTs examination in massively farmed bivalves,we fed three commercially important bivalves,Yesso scallop Patinopecten yessoensis,Pacific oyster Crassostrea gigas,and blue mussel Mytilus edulis with PSTs-producing dinoflagellate Alexandrium catenella,and detected PSTs concentration in different tissues.For all three bivalve species,the digestive gland accumulated much more PSTs than other tissues,and the digestive gland’s toxicity was significantly correlated with the PSTs toxicity of the whole soft-tissues,with r^(2)=0.94,0.92,and 0.94 for Yesso scallop,Pacific oyster,and blue mussel,respectively.When the toxicity of the whole soft-tissues reached 80μgSTXeq(100g)^(−1),the regulatory limit for commercial shellfish,the digestive gland’s toxicity reached 571.48,498.90,and 859.20μgSTXeq(100g)^(−1) in Yesso scallop,Pacific oyster,and blue mussel,respectively.Our results indicate that digestive gland can be used for the sensitive and cost-effective monitoring of PSTs in bivalves.
文摘AIM: To assess current practice of United Kingdom cardiologists with respect to patients with reported shellfish/iodine allergy, and in particular the use of iodinated contrast for elective coronary angiography.Moreover we have reviewed the current evidence-base and guidelines available in this area.METHODS: A questionnaire survey was send to 500senior United Kingdom cardiologists(almost 50% cardiologists registered with British Cardiovascular Society)using email and first 100 responses used to analyze practise. We involved cardiologists performing coronary angiograms routinely both at secondary and tertiary centres. Three specific questions relating to allergy were asked:(1) History of shellfish/iodine allergy in pre-angiography assessment;(2) Treatments offeredfor shellfish/iodine allergy individuals; and(3) Any specific treatment protocol for shellfish/iodine allergy cases. We aimed to establish routine practice in United Kingdom for patients undergoing elective coronary angiography. We also performed comprehensive PubMed search for the available evidence of relationship between shellfish/iodine allergy and contrast media.RESULTS: A total of 100 responses were received, representing 20% of all United Kingdom cardiologists. Ninety-three replies were received from consultant cardiologists, 4 from non-consultant grades and 3 from cardiology specialist nurses. Amongst the respondents, 66% routinely asked about a previous history of shellfish/iodine allergy. Fifty-six percent would pre-treat these patients with steroids and anti-histamines. The other 44% do nothing, or do nonspecific testing based on their personal experience as following:(1) Skin test with 1 mL of subcutaneous contrast before intravenous contrast;(2) Test dose 2 mL contrast before coronary injection;(3) Close observation for shellfish allergy patients; and(4) Minimal evidence that the steroid and anti-histamine regime is effective but it makes us feel better.CONCLUSION: There is no evidence that allergy to shellfish alters the risk of reaction to intravenous contrast more than any other allergy and asking about such allergies in pre-angiogram assessment will not provide any additional information except propagating the myth.
基金The Public Science and Technology Research Funds Projects of Ocean under contract Nos 201305007 and 201405017
文摘Paralytic shellfish toxins(PSTs) are notorious neurotoxins that threaten public health and food safety worldwide.Although PST monitoring programs have recently been established throughout China, the profiles and variation of PSTs in important commercial clams(e.g., Mactra veneriformis, Ruditapes philippinarum, and Meretrix meretrix) along the Jiangsu Province coastline remain largely unexplored. In this study, a validated hydrophilic interaction liquid chromatography–tandem mass spectrometry(HILIC-MS/MS) method was used to examine PST profiles and levels in 540 clam samples from natural production areas along Jiangsu Province coastline during2014–2016. Although the PST levels(≤6.38 μg saxitotoxin equivalents(eq)/kg) were consistently below European Union regulatory limits(≤800 μg saxitotoxin eq/kg) during this time period, saxitotoxin, decarbamoylsaxitotoxin,and gonyautoxins 1 and 4 were detected, and nearly 40% of the samples were saxitotoxin-positive. The PST levels also varied significantly by seasons, with peak values observed in May during 2014–2016. This is the first systematic report of PSTs in clams from Jiangsu Province, and additional research and protective measures are needed to ensure the safety of clams harvested in this area.
基金Supported by the National Key Technology Research and Development Program of China(No.2011BAD13B06)the National Natural Science Foundation of China(No.41276172)the Special Scientific Research Funds For Central Non-Profit Institute,CAFS(No.2014A01YY01)
文摘We measured the organic content and sinking velocities of biodeposits from two scallop species(Chlamys farreri,Patinopecten yessoensis) and abalone(Haliotis discus hannai) that were cultured on suspended long-lines.Measurements were conducted every two months from April 2010 to February 2011.The shellfish were divided into three size groups(small,middle,and big sizes).At each sample point,we assessedbiodeposit organic content,average sinking velocity,the frequency distribution of sinkingvelocities,and the correlation between organic content and sinking velocity.The organic content of biodeposits varied significantly among months(P<0.05) and the pattern of change varied among species.Sinking velocities varied significantly,ranging from <0.5 cm/s to >1.9 cm/s.The sinking velocities of biodeposits from C.farreri and P.yessoensis were 0.5-1.5 cm/s and from H.discus hannai were <0.7 cm/s.The organic content was significantly negatively correlated to the sinking velocity of biodeposits in C.farreri(P<0.001) and P.yessoensis(P<0.05).
基金This work was supported by Fonds Europeen Pour les Affaires Maritimes et la P^eche(FEAMP)(Grant No.R FEA 4700 16FA 1000001).
文摘The process of ultrafiltration(UF)of natural seawater often encounters the problems of variation in water quality and coastal blooms.To validate the feasibility of UF in shellfish farms,this study compared the hydraulic performance and pollutant removal efficiency of the UF process with those of the commonly used treatments that combine several filtration steps with ultraviolet(UV)disinfection.The comparison was conducted in the cases of natural seawater and a coastal bloom.Given that the UF process encountered the specific type of pollution,this study evaluated the filtration performance of the UF process and the retention of total suspended solids(TSS),bacteria,phytoplankton,and zooplankton.A real coastal bloom was considered in the case study of an experimental shellfish hatchery/nursery in France.The results show that both treatments were able to eliminate approximately 50%of TSS.However,in contrast with UV treatment combined with filtration,the UF process retained total amounts of phytoplankton,zooplankton,and bacteria in the bloom.Although the hydraulic performance of the UF process was impacted by the coastal bloom,the fouling was eliminated through chemical cleaning conducted at a frequency less than once per 12 h.Despite the severe pollution,this study confirmed the pollution resistance and treatment performance of the UF process,indicating that UF has the potential to enhance the biosecurity level.
文摘Eelgrass species worldwide are valued for the ecosystem service they provide to estuarine and marine habitats. One species, Zostera japonica, however, has some negative impacts outside its native range and is considered invasive. In Willapa Bay WA, USA, the nonnative eelgrass has expanded to the level where the shellfish industry is concerned about its potential impacts on its livelihood. Studies were conducted using paired plots, Z. japonica controlled with the herbicide imazamox vs. untreated controls, to assess the effects of Z. japonica on Manila clams (Ruditapes philippinarum) and Pacific oysters (Crassostrea gigas). Recruitment of new Manila clams was not affected by Z. japonica. The growth of young clams, total commercial clam harvests, clam quality and clam harvest efficiency, however, were greater on plots where Z. japonica was chemically controlled than where it was not treated. The response of oysters to Z. japonica control varied by site;there was no effect at one site, while the other sites had a 15% increase in shucked meat with Z. japonica control. The potential economic impact of a Z. japonica infestation of a shellfish bed was ~$47,000 ha-1 for Manila clams and $4000 ha-1 for oysters for each crop harvest cycle.
文摘Objective To develop an ICR (female) mouse bioassay (MBA) for toxicity confirmation and evaluation of neurotoxins (brevetoxins)-contaminated shellfish. Methods Brevetoxins (BTX-B) as a causative agent of neurotoxic shellfish poisoning (NSP) under different shellfish matrices were intraperitoneally injected at different doses into mice to study their toxic effects and to differentiate the range of lethal and sublethal dosages. Their sensitivity and specificity were analyzed with 2 competitive ELISA kits for quantitative determination of standard BTX-B and dihydroBTX-B under different shellfish matrix-diluent combinations. Detection rates of MBA and two antibody-based assays for BTX-B from field NSP-positive shellfish samples were compared. Results BTX-B could be detected in shellfish tissues at concentration of 50-400 μg/100 g under shellfish matrix-Tween-saline media, which were appropriate to identify toxic shellfish at or above the regulatory limit (80 μg/100 g shellfish tissues). The LD 50 identified was 455 g/kg for BTX-B under general shellfish matrices (excluding oyster matrices) dissolved in Tween-saline. The presence of shellfish matrices, of oyster matrices in particular, retarded the occurrence of death and toxicity presentation in mice. Two antibody-based assays, even in the presence of different shellfish matrix-diluent combinations, showed acceptable results in quantifying BTX-B and dihydroBTX-B well below the regulatory limit. Conclusion The two ELISA analyses agree favorably (correlation coefficient, r 0.96; Student's t-tests, P>0.05) with the developed bioassay.
基金The project supported by National Natural Science Foundation of China(NSFC)
文摘In this article we explored the crystal structure of a few pearls and shellfish’s shell of fresh water and sea water, and found that pearls and nacreous layer (perhaps and prismatic layer) of shells are all the same: CaCO3, orthorhombic system, aragonite structure and the value of lattice spacings are almost identical. This shows that the pearls of fresh water and sea water are all the same in nature.
基金This work was supported by research grants from the Fundamental Research Funds for the Central Universities(No.201762014)the National Natural Science Foundation of China(No.31772414)the National Natural Science Foundation of Qingdao City(No.20-3-4-16-nsh).
文摘The dried shellfish products with rich nutrients and low-calorie content are favorite food in China,especially in coastal areas.However,the species of dried shellfish products in the market are usually unknown,as the taxonomic features were removed during the production process.This study described the application of DNA barcoding technique to the identification of 100 dried shellfish(scallop,squid,octopus and cuttlefish)products in markets.Samples were authenticated by comparing mitochondrial cytochrome oxidase subunit I(COI)gene and 16S ribosomal RNA(16S rRNA)gene sequences with public reference taxonomic databases.The results showed that all the 100 products can be identified at species level.Sixty four scallop adductor products were processed using the bay scallop,Argopecten irradians,and one was from Portuguese oyster,Crassostrea angulata.All the 27 squid,2 cuttlefish and 6 octopus products were produced by the Jumbo flying squid,Dosidicus gigas.The neighbour-joining tree is in agreement with the results of DNA barcoding analysis.The 64 scallop samples formed one A.irradians cluster,leaving Sca65 clustered with the reference oyster sequence C.angulata(MH997922).All the 35 cephalopod(squid,octopus and cuttlefish)samples formed a D.gigas cluster.This investigation revealed a low variety of dried shellfish products sold on the market,and highlighted the high rate of mislabeling and species substitution.Our present work provides a practical method for tracing and authenticating shellfish products.
文摘By using a farm's data in Yantai City and the theory of Cost-Volume-Profit analysis and the financial management methods,this paper construct a multi-factor analysis model for improving profit management using Excel 2007 in Shellfish farming projects and describes the procedures to construct a multi-factor analysis model.The model can quickly calculate the profit,improve the level of profit management,find out the breakeven point and enhance the decision-making efficiency of businesses etc.It is also a thought of the application to offer suggestions for government decisions and economic decisions for corporations as a simple analysis tool.While effort has been exerted to construct a four-variable model,some equally important variables may not be discussed sufficiently due to limitation of the paper's space and the authors'knowledge.All variables can be listed in EXCEL 2007 and can be associated in a logical way to manage the profit of shellfish farming projects more efficiently and more practically.
文摘Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">)</span></span></span><span><span><span style="font-family:""> by canning via three treatments </span></span></span><span><span><span style="font-family:"">that </span></span></span><span><span><span style="font-family:"">are canned raw, canned smoked and canned cooked as a healthy product with high nutritional value. Results<span> </span>reported that edible meat of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> was free from Cu, Pb and Cd as a result of washing process. There was significantly (p < 0.05) higher in moisture content of canned raw compared with other tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">, and there was significantly reduction of protein content (p < 0.05) in tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked compared with the canned raw. Significant different (p < 0.05) in fat content </span></span></span><span><span><span style="font-family:"">w</span></span></span><span><span><span style="font-family:"">as canned raw < canned smoked < canned cooked. On the other side</span></span></span><span><span><span style="font-family:"">,</span></span></span><span><span><span style="font-family:""> the three treatments of canned shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:"">had significant differen</span></span></span><span><span><span style="font-family:"">ce</span></span></span><span><span><span style="font-family:""> (p < 0.05) in content of ash and carbohydrates content. Canned smoked of shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> showed the lowest value of pH. All treatments of canned shellfish<span> </span></span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> were rich in minerals where canned raw treatment was higher in Ca, Fe, Zn, Mg, and Mn. While canned smoked had minerals content lower than canned raw. Lightness (L*) had slightly differen</span></span></span><span><span><span style="font-family:"">ce</span></span></span><span><span><span style="font-family:""> between all treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned shellfish </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia</span></i></span></span><span><span><i><span style="font-family:""> <span>undulate</span></span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">. Reduction on lightness and redness was noticed at the same time in all treatments of canned shellfish<span> </span></span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia</span></i></span></span><span><span><span style="font-family:""> <i>undulate</i></span></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">,</span></span></span><span><span><span style="font-family:""> canned raw had</span></span></span><span><span><span style="font-family:""> the </span></span></span><span><span><span style="font-family:"">highest value of lightness (L*), and redness (a*). Canned smoked had the highest value of hardness followed by canned raw then canned cook. Slightly significant difference (p < 0.05) was noticed between three treatments in sensory properties, data showed that all panelists prefer</span></span></span><span><span><span style="font-family:"">red</span></span></span><span><span><span style="font-family:""> canned cooked than raw canned and canned smoked, degree of canned cooked was excellent.
文摘Our study investigated the effect of utiliz</span></span><span><span><span style="font-family:;" "="">ing</span></span></span><span><span><span style="font-family:;" "=""> chicken</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">wings and dehydrated shellfish to produce chicken nuggets rich in protein, minerals and vitamins.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">The proximate composition, nutritional value, physical and sensorial properties were evaluated. The different</span></span></span><span><span><span style="font-family:;" "="">ly</span></span></span><span><span><span style="font-family:;" "=""> prepared chicken nuggets </span></span></span><span><span><span style="font-family:;" "="">were </span></span></span><span><span><span style="font-family:;" "="">supplemented with dehydrated shellfish CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">2%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% and CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6%. There was </span></span></span><span><span><span style="font-family:;" "="">a </span></span></span><span><span><span style="font-family:;" "="">significantly reduction </span></span></span><span><span><span style="font-family:;" "="">in </span></span></span><span><span><span style="font-family:;" "="">moisture and </span></span></span><span><span><span style="font-family:;" "="">an </span></span></span><span><span><span style="font-family:;" "="">increasing in protein content (<i>P</i> < 0.05) in all Chicken Nuggets (CN) in comparison to the control. Fat content was significantly higher in CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0% than CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">2%, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% and CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6%. Ash content was increasing with added dehydrated shellfish, while </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">reduction in carbohydrates and total calories of the different chicken nuggets were noticed with </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">significant gradual rise in pH values, TBA values showed no significant difference (<i>P</i> < 0.05) between chicken nuggets. Results showed that using dehydrated shellfish in preparing chicken nuggets caused a marked rise in its content of Ca, Fe, Na, K, Zn, Mg and Mn levels, CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0% had the lowest concentration of all minerals, and had lower value</span></span></span><span><span><span style="font-family:;" "="">s</span></span></span><span><span><span style="font-family:;" "=""> of vitamins A, E and D but CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6% had higher value</span></span></span><span><span><span style="font-family:;" "="">s</span></span></span><span><span><span style="font-family:;" "=""> of vitamins A, E and D. Control Nuggets (CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">0%) had the lowest value of yellowness and highest value of lightness but CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">6% had </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">highest value of yellowness and, </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">lowest value of lightness. </span></span></span><span><span><span style="font-family:;" "="">The </span></span></span><span><span><span style="font-family:;" "="">redness had </span></span></span><span><span><span style="font-family:;" "="">a </span></span></span><span><span><span style="font-family:;" "="">slightly reduction. All samples showed slight changes in</span></span></span><span><span><span style="font-family:;" "=""> hardness, cohesiveness, springiness, gumminess and chewiness were noticed. </span></span></span><span><span><span style="font-family:;" "="">An </span></span></span><span><span><span style="font-family:;" "="">increase in WHC, pick-up, and cooking loss of chicken nuggets with dehydrated shellfish was noticed. Panelists accepted all prepared chicken nuggets and the chicken nuggets CN</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "="">4% had </span></span></span><span><span><span style="font-family:;" "="">the </span></span></span><span><span><span style="font-family:;" "="">highest score compared with other chicken nuggets prepared.
文摘Antibiotic resistant bacteria pathogens remain the leading cause of shellfish borne diseases and a major health threat to humans worldwide. The objectives of this study were to isolate, identify, and determine the antibiotic resistance patterns of Gram-negative bacteria from shellfish. We analyzed a total of 540 shellfish (117 clams, 88 oysters, and 136 periwinkles) samples collected from different vendors at Iko and Douglas Creeks in Akwa Ibom State, South-South Nigeria. Conventional cultural techniques, morphological, biochemical characteristics, and PCR amplification were used to identify the bacterial isolates. Antibiotic susceptibility tests (Kirby-Bauer disk diffusion method) and ESBL phenotype (disk) of the isolates were performed. One hundred and thirty-five (135) Gram-negative bacteria comprising 5 genera and 14 species were detected at a prevalence of: <i>Alcaligenes faecalis</i> <i><b>TRB</b></i>-7 38 (28.2%), <i>Pseudomonas oryzihabitans strain <b>KCB</i>005</b> 16 (11.9%), <i>Paenalcaligenes retgerii strain <b>B</i>5</b> 12 (8.9%) <i>Pseudomonas aeruginosa <b>JB</i>2</b> 10 (7.4%), <i>Providencia stuartii <b>DMC</i>-28b</b> 9 (6.7%), <i>Alcaligenes species <b>TLT</i>151</b> 8 (5.9%), <i>Pseudomonas aeruginosa <b>CIFRI DTSB</i>1</b> 7 (5.2%), <i>Paenalcaligenes species <b>UN</i>24</b> 7 (5.2%), <i>Alcaligenes faecalis <b>BT</i>10</b> 7 (5.2%), <i>Vibrio species strain <b>PrVy</i>108</b> 6 (4.4%), <i>Pseudomonas xiamenensis <b>C</i>10-2</b> 5 (3.7%), <i>Providencia vemicola <b>Bu</i>15_38</b> 4 (2.9%), <i>Pseudomonas anguillisceptica</i> <b>4029</b> 3 (2.2%), and <i>Pseudomonas aeruginosa <b>N</i>15-01092</b> 3 (2.2%). All tested isolates showed various degrees of resistance to the thirteen antimicrobials evaluated. High levels of resistance (100%) to cefepime and imipenem were expressed by all isolates except the <i>Providencia</i> species. For the EBSL indicators, all isolates apart from <i>Alcaligenes</i> species were resistant (100%) to ceftriaxone. All <i>Vibrio</i> species were susceptible to norfloxacin, nalidixic acid, and ceftazidime. The identification of antibiotic resistant Gram-negative bacteria (GNARB) from shellfish in this study highlights the risk of disseminated multi-drug resistance—a serious public health concern.
文摘Abdul Owor,a seasoned fisherman carries his day’s catch of lobsters along Mombasa beach and takes it to a nearby store room for sale.The store room is a lobster collection center belonging to a Chinese company that deals in the export of live lobsters.Owor ’s catch is weighed and he is paid cash by the Kenyan attendant.Owor said that unlike in the past when he had to depend on Kenyan middlemen to market his lobsters.
文摘Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction(RT-ddPCR). Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption.
基金The work was supported by National Basic Research Project No. 2001 CB409700, NNSFC KZCX2-YW-208.
文摘Objective To study the transfer of paralytic shellfish toxins (PST) using four simulated marine food chains: dinoflagellate Alexandrium tamarense→Artemia Artemia salina→Mysid shrimp Neomysis awatschensis; A. tamarense→N. awatschensis; A. tamarense→A. salina→Perch Lateolabrax japonicus; and A. tamarense→L. japonicus. Methods The ingestion of A. tamarense, a producer of PST, by L. japonicus, N. awatschensis, and A. salina was first confirmed by microscopic observation of A. tamarense cells in the intestine samples of the three different organisms, and by the analysis of Chl.a levels in the samples. Toxin accumulation in L. japonicus and N. awatschensis directly from the feeding on A. tamarense or indirectly through the vector of A. salina was then studied. The toxicity of samples was measured using the AOAC mouse bioassay method, and the toxin content and profile of A. tamarense were analyzed by the HPLC method. Results Both A. salina and N. awatschensis could ingest A. tamarense cells. However, the ingestion capability of A. salina exceeded that of N. awatschensis. After the exposure to the culture of A. tamarense (2 000 cells·mL-1) for 70 minutes, the content of Chl.a in A. salina and N. awatschensis reached 0.87 and 0.024 μg·mg-1, respectively. Besides, A. tamarense cells existed in the intestines of L. japonicus, N. awatschensis and A. salina by microscopic observation. Therefore, the three organisms could ingest A. tamarense cells directly. A. salina could accumulate high content of PST, and the toxicity of A. salina in samples collected on days 1, 4, and 5 of the experiment was 2.18, 2.6, and 2.1 MU·g-1, respectively. All extracts from the samples could lead to death of tested mice within 7 minutes, and the toxin content in artemia sample collected on the 1st day was estimated to be 1.65×10-5 μg STX equal/individual. Toxin accumulation in L. japonicus and N. awatschensis directly from the feeding on A. tamarense or indirectly from the vector of A. salina was also studied. The mice injected with extracts from L. japonicus and N. awatschensis samples that accumulated PST either directly or indirectly showed PST intoxication symptoms, indicating that low levels of PST existed in these samples. Conclusion Paralytic shellfish toxins can be transferred to L. japonicus, N. awatschensis, and A. salina from A. tamarense directly or indirectly via the food chains.
