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Study on Shoot Tip Culture of Illciaceae Ornamental Plants
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作者 方向明 赵后斌 +2 位作者 刘文倩 郑必平 谈建中 《Agricultural Science & Technology》 CAS 2012年第4期735-738,759,共5页
[Objective] This study aimed to establish a technology system for tissue culture and rapid propagation of Illciaceae ornamental plants. [Method] Effects of medium components and anti-browning agents on the survival an... [Objective] This study aimed to establish a technology system for tissue culture and rapid propagation of Illciaceae ornamental plants. [Method] Effects of medium components and anti-browning agents on the survival and growth of shoot tips were investigated by using apical buds of IItciaceae plant Haierlian as experiment material and MS as basic medium. [Result] The results showed that apical buds at the early germination period in spring were the most suitable explants for tissue culture of IIIciaceae plant Haierlian. Sterilization with 0.1% HgCI2 for 6 min achieved the best effect, while conventional surface-sterilization with ethanol would affect the survival of explants. The optimal medium for primary culture was MS-D (with modifications in major elements and organic components) + anti-browning agents (equa~ volume) + 2.0 mg/L of 6-BA + 0.5 mg/L of NAA. The optimal subculture medi- um was MS-F (with modifications in inorganic and organic components) + anti-brown- ing agents (equal volume) + 2.0 mg/L of 6-BA + 0.1 mg/L of NAA. [Conclusion] This study laid the foundation for establishment of tissue culture and rapid propagation technology system for Haierlian. 展开更多
关键词 Illciaceae plant Haieflian shoot tip culture Sterile system Tissue browning
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Effect of Cytokinins on the Micropropagation of Carob(Ceratonia siliqua L.)through Shoot Tip Culture 被引量:1
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作者 Rabah Saidi Safaa Rahmouni +3 位作者 Zineb Nejjar El Ansari Abdelfettah Maouni Alain Badoc Ahmed Lamarti 《American Journal of Plant Sciences》 2019年第9期1469-1481,共13页
In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedl... In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l);high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l. 展开更多
关键词 CAROB Ceratonia siliqua L. CYTOKININS shoot tip Culture
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Contributions of Chinese Botanists to Plant Tissue Culture in the 20th entury 被引量:24
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作者 朱至清 《Acta Botanica Sinica》 CSCD 2002年第9期1075-1084,共10页
This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee... This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced. 展开更多
关键词 Plant tissue culture ORGANOGENESIS shoot tip culture anther culture ovary culture endosperm culture protoplast culture plant mass cell culture
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