Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neu- ~ ~ ~ 2＋ ~ ~ 2＋ rotransmlsslon by decreasing Ca reflux through high voltage-gated Ca channels. However, recent studies...Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neu- ~ ~ ~ 2＋ ~ ~ 2＋ rotransmlsslon by decreasing Ca reflux through high voltage-gated Ca channels. However, recent studies suggest that cannabinoids which activate cannabinoid receptor type 1 can increase neurotransmitter release by enhancing Ca2＋ influx in vitro. The aim of the present study was to investigate the modulation of intracellular Ca2＋ concentration by the cannabinoid receptor type 1 agonist anandamide, and its underlying mechanisms. Using whole cell voltage-damp and calcium imaging in cultured trigeminal ganglion neurons, we found that anandamide directly caused Ca2＋ influx in a dose-dependent manner, which then triggered an increase of intracellular Ca2＋ concentration. The cyclic adenosine and guanosine monophosphate-dependent protein kinase systems, but not the protein kinase C system, were involved in the increased intracellular Ca2＋concentration by anandamide. This result showed that anandamide increased intracellu- lar Ca2＋ concentration and inhibited high voltage-gated Ca2＋ channels through different signal transduction pathways.展开更多
Difficult intubation, inadequate ventilation and esophageal intubation are the principal causes of death or brain damage related to airway manipulation. The objective of this cross-sectional study was to correlate a p...Difficult intubation, inadequate ventilation and esophageal intubation are the principal causes of death or brain damage related to airway manipulation. The objective of this cross-sectional study was to correlate a preanesthetic evaluation that may be capable of predicting a difficult intubation with the conditions encountered at laryngoscopy and endotracheal intubation. Eighty-one patients submitted to general anesthesia were evaluated at a preanesthetic consultation according to the modified Mallampati classification, the Wilson score and the American Society of Anesthesiologists (ASA) difficult airway algorithm. Findings were then correlated with the Cormack-Lehane classification and with the number of attempts at endotracheal intubation. No statistically significant correlations were found between the patients’ Mallampati classification and their Cormack-Lehane grade or between the Mallampati classification and the number of attempts required to achieve endotracheal intubation. Laryngoscopy proved difficult in four patients and in all of these cases the Wilson score had been indicative of a possibly difficult airway, highlighting its good predicting sensitivity. However, the specificity of this test was low, since another 24 patients had the same Wilson score but were classified as Cormack-Lehane I/II. Moreover, two patients who had a Wilson score ≥ 4 were also classified as Cormack-Lehane grade I/II. The study concluded that the Wilson score, although seldom used in clinical practice, is a highly sensitive predictor of a difficult airway;its specificity, however, is low.展开更多
A recent technique, using to potentially replace the conventional impregnating one in low voltage electric motors, has been studied in this communication. The conventional technique that uses?both primary and secondar...A recent technique, using to potentially replace the conventional impregnating one in low voltage electric motors, has been studied in this communication. The conventional technique that uses?both primary and secondary insulations;?i.e.: enamel and varnish, has been compared to this recent technique which only uses one component,?i.e.: self-bonding wires. Self-bonding wires polymerization is very quick compared to that of the conventional technique. Environmental impact and both dielectric and mechanical properties of these two techniques have been estimated and compared. The goal is to estimate if this recent technique has not only a better environmental footprint but also good technical properties. The dielectric properties that have been measured are the dielectric strength and?the lifetime under pulse?voltage while mechanical consists in measuring the bonding strength that is crucial for impregnation.?For that purpose, a Life Cycle Assessment (LCA) that can simultaneously evaluatenumerous impacts on ground, water and air,?has been performed. The results show clearly that the recent technique significantly reduces the environmental footprint. Both mechanical and dielectric properties are then compared and analyzed.展开更多
Minimally invasive surgery started spreading worldwide in 1987,when the first laparoscopic cholecystectomy was performed.Meanwhile,improvement of endoscopic equipment and instruments allowed gastroenterologists to att...Minimally invasive surgery started spreading worldwide in 1987,when the first laparoscopic cholecystectomy was performed.Meanwhile,improvement of endoscopic equipment and instruments allowed gastroenterologists to attempt more aggressive endoluminal interventions,even beyond the wall barrier.The first transgastric peri-toneoscopy,in 2004,brought to light the concept of natural orifice transluminal endoscopic surgery(NOTES).The idea of incisionless surgery is attractive and has become a new goal for both surgeons and other people interested in this field of investigation.The authors present a review of all developments concerning NOTES,including animal studies and human experience.展开更多
Internet of Things (IoT) is ubiquitous, including objects or devices communicating through heterogenous wireless networks. One of the major challenges in mobile IoT is an efficient vertical handover decision (VHD) tec...Internet of Things (IoT) is ubiquitous, including objects or devices communicating through heterogenous wireless networks. One of the major challenges in mobile IoT is an efficient vertical handover decision (VHD) technique between heterogenous networks for seamless connectivity with constrained resources. The conventional VHD approach is mainly based on received signal strength (RSS). The approach is inefficient for vertical handover, since it always selects the target network with the strongest signal without taking into consideration of factors such as quality of service (QoS), cost, delay, etc. In this paper, we present a hybrid approach by integrating the multi-cri- teria based VHD (MCVHD) technique and an algorithm based on fuzzy logic for efficient VHD among Wi-Fi, Radio and Satellite networks. The MCVHD provides a lightweight solution that aims to achieving seamless connectivity for mobile IoT Edge Gateway over a set of heterogeneous networks. The proposed solution is evaluated in real time using a testbed containing real IoT devices. Further, the testbed is integrated with lightweight and efficient software techniques, e.g., microservices, containers, broker, and Edge/Cloud techniques. The experimental results show that the proposed approach is suitable for an IoT environment and it outperforms the conventional RSS Quality based VHD by minimizing handover failures, unnecessary handovers, handover time and cost of service.展开更多
Polarimetry plays an important role in the measurement of solar magnetic fields. We devel- oped a high-sensitivity and high-accuracy polarimeter （HHP） based on nematic liquid crystal variable retarders （LCVRs）, wh...Polarimetry plays an important role in the measurement of solar magnetic fields. We devel- oped a high-sensitivity and high-accuracy polarimeter （HHP） based on nematic liquid crystal variable retarders （LCVRs）, which has a compact setup and no mechanical moving parts. The system design and calibration methods are discussed in detail. The azimuth error of the transmission axis of the polarizer as well as the fast axes of the two LCVRs and the quarter-wave plate were determined using dedicated procedures. Linearly and circularly polarized light were employed to evaluate the performance of the HHP. The experimental results indicate that a polarimetric sensitivity of better than 5.7 × 10-3 can be achieved by using a single short-exposure image, while an accuracy on the order of 10-5 can be reached by using a large number of short-exposure images. This makes the HHP a high-performance system that can be used with a ground-based solar telescope for high-precision solar magnetic field investigations.展开更多
目的探讨利用间隔采集与连续采集模式行单采血小板采集后,对献血者外周血小板(P L T)的影响。方法选择自愿进行单采血小板献血者54例,将其分为2组,分别行间隔采集模式(2 U/次→1 U/次→2 U/次)与连续采集模式(2U/次),对两组对象采集前...目的探讨利用间隔采集与连续采集模式行单采血小板采集后,对献血者外周血小板(P L T)的影响。方法选择自愿进行单采血小板献血者54例,将其分为2组,分别行间隔采集模式(2 U/次→1 U/次→2 U/次)与连续采集模式(2U/次),对两组对象采集前、后以及采集后12个月的平均血小板体积(M P V)、P L T、血小板分布宽度(P D W)、大血小板比率(P-LCR)以及身体质量指数(BMI)的变化进行分析。结果连续模式2次单采前PLT水平低于间隔模式,差异有统计学意义(P<0.05);连续模式采集12个月后,MPV、P-LCR、PDW与间隔模式比较,均明显降低,差异有统计学意义(P<0.05);连续模式BMI≥24高于间隔模式,差异有统计学意义(P<0.05)。结论单采血小板采集应结合捐献者的PLT与BMI等指标进行综合考虑,若捐献者BMI≥24,建议采取间隔采集模式。展开更多
An enhanced differential transform method （EDTM）, which introduces the Pad@ technique into the standard differential transform method （DTM）, is proposed. The enhanced method is applied to the analytic treatment of...An enhanced differential transform method （EDTM）, which introduces the Pad@ technique into the standard differential transform method （DTM）, is proposed. The enhanced method is applied to the analytic treatment of the shock wave. It accelerates the convergence of the series solution and provides an exact Dower series solution.展开更多
The application of flue-cured tobacco well-cellar transplanting technique un- der film and covering bowl in southwest prefecture of Guizhou province was intro- duced. And the two flue-cured tobacco well-cellar transpl...The application of flue-cured tobacco well-cellar transplanting technique un- der film and covering bowl in southwest prefecture of Guizhou province was intro- duced. And the two flue-cured tobacco well-cellar transplanting techniques were compared. Results showed that compared with flue-cured tobacco well-collar trans- planting technique under film, the technique covering bowl had the advantages of protecting ecological environment, reducing labor force and cost, resisting the bad weather and so on. This research provided theoretical foundation for the early appli- cation flue-cured tobacco well-cellar transplanting technique covering bowl in different展开更多
We present the results obtained from extensive near-infrared （IR） spectro- scopic and photometric observations of the Be/X-ray binary A0535＋262/HDE 245770 at different phases of its - 111 d orbital period. This obs...We present the results obtained from extensive near-infrared （IR） spectro- scopic and photometric observations of the Be/X-ray binary A0535＋262/HDE 245770 at different phases of its - 111 d orbital period. This observation campaign is part of the monitoring program of selective Be/X-ray binary systems aimed at understanding X-ray and near-IR properties at different orbital phases, especially during the perias- tron passage of the neutron star. The near-IR observations presented here were carried out using the 1.2 m telescope at the Mt. Abu IR Observatory. Though the source was relatively faint for spectroscopic observations with the 1.2 m telescope, we monitored the source closely during the 2011 February-March giant X-ray outburst to primarily investigate whether any drastic changes in the near-IR JHK spectra took place at the periastron passage. Changes of such a striking nature were expected to be detectable in our spectra. Photometric observations of the Be star show a gradual and systematic fading in the JHK light curves since the onset of the X-ray outburst, which could suggest a mild evacuation/truncation of the circumstellar disk of the Be companion. Near-IR spectroscopy of the object shows that the JHK spectra are dominated by the emission lines of hydrogen Brackett and Paschen series and HeI lines at 1.0830, 1.7002 and 2.0585 μm. The presence of all the hydrogen emission lines in the JHK spectra, along with the absence of any significant change in the continuum of the Be companion during X-ray quiescent and X-ray outburst phases, suggests that the near- IR line emitting regions of the disk are not significantly affected during the X-ray outburst.展开更多
The one-meter telescope at Weihai Observatory （WHO） of Shandong University is an f/8 Cassegrain telescope. Three sets of filters are installed in a dual layer filterwheel that use Johnson-Cousins UBVRI, Sloan Digita...The one-meter telescope at Weihai Observatory （WHO） of Shandong University is an f/8 Cassegrain telescope. Three sets of filters are installed in a dual layer filterwheel that use Johnson-Cousins UBVRI, Sloan Digital Sky Survey u′g′r′i′z′ and Stomgren uvby. The photometric system and the CCD camera are introduced, followed by detailed analysis of their performances, and determination of the relevant parameters, including gain, readout noise, dark current and linearity of the CCD cam- era. In addition, the parameters describing the site＇s astro-climate, including typical seeing, statistics on the number of clear nights and average sky brightness, based on data gathered from Sep. 2007 to Aug. 2013, are systematically studied and reported in this work. Photometric calibrations were done using Landolt standard star obser- vations spanning eight nights, which yielded transformation coefficients, photometric precision and system throughput. The limiting magnitudes are simulated using the derived calibration parameters and classic observation conditions at WHO.展开更多
The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first su...The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submit-ted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as re-stricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microar-ray for hybridization. The diagnostic capability of the mi-croarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microar-ray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide mi-croarray, which can improve the positive ratio of the diagno-sis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status.展开更多
As for unmanned aircraft, the knowledge of the aircraft performance is directly related with the navigation, guidance, and control system programming. Therefore, the measured data in each phase of the flight must be s...As for unmanned aircraft, the knowledge of the aircraft performance is directly related with the navigation, guidance, and control system programming. Therefore, the measured data in each phase of the flight must be sufficiently precise to obtain a good characterization of aircraft.This article proposes new methods of sending information to ground, which make it possible to know the aircraft behavior accurately, and for this purpose, four contributions have been made for ALO(Avión Ligero de Observación, Spanish acronym for Light Observation Aircraft). Currently, the characterization is based on data obtained at ten samples per second, insufficient to acquire detailed knowledge of what happened during the whole flight of an aircraft. As a result of these contributions, many more samples per second of accelerations and angular velocities are obtained at the most critical moments of the flight, such as takeoff or landing. Among the improvements included are data compression techniques, providing references to locate the measured data in time and identifying labels of each parameter.展开更多
The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents(STOCs)in freshly isolated porcine coronary artery smooth muscle cells(ASMCs).STOCs wer...The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents(STOCs)in freshly isolated porcine coronary artery smooth muscle cells(ASMCs).STOCs were recorded using the perforated whole-cell patch-clamp configuration.STOCs were voltage-dependent and superimposed stochastically onto whole-cell Ca^(2+)-activated-K+(BKCa)currents.Charybdotoxin(ChTX,200 nmol/L),a selective blocker of BKCa channels,completely inhibited STOCs within 10 min.STOCs activity was greatly suppressed when extracellular Ca^(2+)concentration decreased from 1.8 mmol/L to 200 nmol/L,further removal of Ca^(2+)abolished STOCs activity.Ca^(2+)ionophore A23187(10μmol/L)increased STOCs activity significantly.Verapamil(20μmol/L)and CdCl2(200μmol/L),two kinds of organic L-type voltage-dependent Ca^(2+)channels(L-VDCCs)antagonists,had little effect on STOCs.In addition,the ryanodine receptors(RyRs)agonist caffeine(5 mmol/L)significantly activated STOCs.Application of ryanodine(50μmol/L)to block RyRs abolished STOCs,subsequent washout of ryanodine or application of caffeine failed to reproduce STOCs activity.Inhibition of inositol 1,4,5-trisphosphate receptors(IP_(3)Rs)by 2APB(40μmol/L)greatly suppressed the activity of STOCs,application of caffeine(5 mmol/L)in the presence of 2APB caused a burst of outward currents followed by inhibition of STOCs.These results suggest that STOCs in porcine coronary ASMCs are mediated by BKCa channels.Extracellular Ca^(2+)is essential for STOCs activity,while Ca^(2+)entry through L-VDCCs has little effect on STOCs.Intracellular Ca^(2+)release induced by RyRs is responsible for the regulation of STOCs,whereas IP_(3)Rs might also be involved.展开更多
Defects in mitochondria are responsible for various genetic and acquired diseases.Mitochondrial transplantation,a method that involves introduction of healthy donor mitochondria into cells with dysfunctional mitochond...Defects in mitochondria are responsible for various genetic and acquired diseases.Mitochondrial transplantation,a method that involves introduction of healthy donor mitochondria into cells with dysfunctional mitochondria,could offer a novel approach to treat such diseases.Some studies have demonstrated the therapeutic benefit of mitochondrial transplantation and targeted delivery in vivo and in vitro within hepatocytes and the liver.This review discusses the issues regarding isolation and delivery of mitochondria to hepatocytes and the liver,and examines the existing literature in order to elucidate the utility and practicality of mito-chondrial transplantation in the treatment of liver disease.Studies reviewed demonstrate that mitochondrial uptake could specifically target hepatocytes,address the challenge of non-specific localization of donor mitochondria,and provide evidence of changes in liver function following injection of mitochondria into mouse and rat disease models.While potential benefits and advantages of mitochondrial trans-plantation are evident,more research is needed to determine the practicality of mitochondrial transplantation for the treatment of genetic and acquired liver diseases.展开更多
基金supported by NIH,grant No.GM-63577NNSF,grant No.30571537,No.30271500+1 种基金the National Natural Science Foundation of China,No.30271500,30571537 and 813702462010 National Clinical Key Disciplines Construction Grant from the Ministry of Health of the People’s Republic of China
文摘Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neu- ~ ~ ~ 2＋ ~ ~ 2＋ rotransmlsslon by decreasing Ca reflux through high voltage-gated Ca channels. However, recent studies suggest that cannabinoids which activate cannabinoid receptor type 1 can increase neurotransmitter release by enhancing Ca2＋ influx in vitro. The aim of the present study was to investigate the modulation of intracellular Ca2＋ concentration by the cannabinoid receptor type 1 agonist anandamide, and its underlying mechanisms. Using whole cell voltage-damp and calcium imaging in cultured trigeminal ganglion neurons, we found that anandamide directly caused Ca2＋ influx in a dose-dependent manner, which then triggered an increase of intracellular Ca2＋ concentration. The cyclic adenosine and guanosine monophosphate-dependent protein kinase systems, but not the protein kinase C system, were involved in the increased intracellular Ca2＋concentration by anandamide. This result showed that anandamide increased intracellu- lar Ca2＋ concentration and inhibited high voltage-gated Ca2＋ channels through different signal transduction pathways.
文摘Difficult intubation, inadequate ventilation and esophageal intubation are the principal causes of death or brain damage related to airway manipulation. The objective of this cross-sectional study was to correlate a preanesthetic evaluation that may be capable of predicting a difficult intubation with the conditions encountered at laryngoscopy and endotracheal intubation. Eighty-one patients submitted to general anesthesia were evaluated at a preanesthetic consultation according to the modified Mallampati classification, the Wilson score and the American Society of Anesthesiologists (ASA) difficult airway algorithm. Findings were then correlated with the Cormack-Lehane classification and with the number of attempts at endotracheal intubation. No statistically significant correlations were found between the patients’ Mallampati classification and their Cormack-Lehane grade or between the Mallampati classification and the number of attempts required to achieve endotracheal intubation. Laryngoscopy proved difficult in four patients and in all of these cases the Wilson score had been indicative of a possibly difficult airway, highlighting its good predicting sensitivity. However, the specificity of this test was low, since another 24 patients had the same Wilson score but were classified as Cormack-Lehane I/II. Moreover, two patients who had a Wilson score ≥ 4 were also classified as Cormack-Lehane grade I/II. The study concluded that the Wilson score, although seldom used in clinical practice, is a highly sensitive predictor of a difficult airway;its specificity, however, is low.
文摘A recent technique, using to potentially replace the conventional impregnating one in low voltage electric motors, has been studied in this communication. The conventional technique that uses?both primary and secondary insulations;?i.e.: enamel and varnish, has been compared to this recent technique which only uses one component,?i.e.: self-bonding wires. Self-bonding wires polymerization is very quick compared to that of the conventional technique. Environmental impact and both dielectric and mechanical properties of these two techniques have been estimated and compared. The goal is to estimate if this recent technique has not only a better environmental footprint but also good technical properties. The dielectric properties that have been measured are the dielectric strength and?the lifetime under pulse?voltage while mechanical consists in measuring the bonding strength that is crucial for impregnation.?For that purpose, a Life Cycle Assessment (LCA) that can simultaneously evaluatenumerous impacts on ground, water and air,?has been performed. The results show clearly that the recent technique significantly reduces the environmental footprint. Both mechanical and dielectric properties are then compared and analyzed.
文摘Minimally invasive surgery started spreading worldwide in 1987,when the first laparoscopic cholecystectomy was performed.Meanwhile,improvement of endoscopic equipment and instruments allowed gastroenterologists to attempt more aggressive endoluminal interventions,even beyond the wall barrier.The first transgastric peri-toneoscopy,in 2004,brought to light the concept of natural orifice transluminal endoscopic surgery(NOTES).The idea of incisionless surgery is attractive and has become a new goal for both surgeons and other people interested in this field of investigation.The authors present a review of all developments concerning NOTES,including animal studies and human experience.
文摘Internet of Things (IoT) is ubiquitous, including objects or devices communicating through heterogenous wireless networks. One of the major challenges in mobile IoT is an efficient vertical handover decision (VHD) technique between heterogenous networks for seamless connectivity with constrained resources. The conventional VHD approach is mainly based on received signal strength (RSS). The approach is inefficient for vertical handover, since it always selects the target network with the strongest signal without taking into consideration of factors such as quality of service (QoS), cost, delay, etc. In this paper, we present a hybrid approach by integrating the multi-cri- teria based VHD (MCVHD) technique and an algorithm based on fuzzy logic for efficient VHD among Wi-Fi, Radio and Satellite networks. The MCVHD provides a lightweight solution that aims to achieving seamless connectivity for mobile IoT Edge Gateway over a set of heterogeneous networks. The proposed solution is evaluated in real time using a testbed containing real IoT devices. Further, the testbed is integrated with lightweight and efficient software techniques, e.g., microservices, containers, broker, and Edge/Cloud techniques. The experimental results show that the proposed approach is suitable for an IoT environment and it outperforms the conventional RSS Quality based VHD by minimizing handover failures, unnecessary handovers, handover time and cost of service.
基金funded by the National Natural Science Foundation of China(NSFC,Grant Nos.11661161011,11433007,11220101001,11328302,11373005 and 11303064)the Opening Project of Key Laboratory of Astronomical Optics&Technology,Nanjing Institute of Astronomical Optics&Technology,Chinese Academy of Sciences(CASKLAOT-KF201606)+4 种基金the“Strategic Priority Research Program”of the Chinese Academy of Sciences(Grant No.XDA04075200)the special fund for astronomy of CAS(2015–2016)the special funding for Young Researcher of Nanjing Institute of Astronomical Optics&Technologythe International Partnership Program of the Chinese Academy of Sciences(Grant No.114A32KYSB20160018)the Mt.Cuba Astronomical Foundation
文摘Polarimetry plays an important role in the measurement of solar magnetic fields. We devel- oped a high-sensitivity and high-accuracy polarimeter （HHP） based on nematic liquid crystal variable retarders （LCVRs）, which has a compact setup and no mechanical moving parts. The system design and calibration methods are discussed in detail. The azimuth error of the transmission axis of the polarizer as well as the fast axes of the two LCVRs and the quarter-wave plate were determined using dedicated procedures. Linearly and circularly polarized light were employed to evaluate the performance of the HHP. The experimental results indicate that a polarimetric sensitivity of better than 5.7 × 10-3 can be achieved by using a single short-exposure image, while an accuracy on the order of 10-5 can be reached by using a large number of short-exposure images. This makes the HHP a high-performance system that can be used with a ground-based solar telescope for high-precision solar magnetic field investigations.
文摘目的探讨利用间隔采集与连续采集模式行单采血小板采集后,对献血者外周血小板(P L T)的影响。方法选择自愿进行单采血小板献血者54例,将其分为2组,分别行间隔采集模式(2 U/次→1 U/次→2 U/次)与连续采集模式(2U/次),对两组对象采集前、后以及采集后12个月的平均血小板体积(M P V)、P L T、血小板分布宽度(P D W)、大血小板比率(P-LCR)以及身体质量指数(BMI)的变化进行分析。结果连续模式2次单采前PLT水平低于间隔模式,差异有统计学意义(P<0.05);连续模式采集12个月后,MPV、P-LCR、PDW与间隔模式比较,均明显降低,差异有统计学意义(P<0.05);连续模式BMI≥24高于间隔模式,差异有统计学意义(P<0.05)。结论单采血小板采集应结合捐献者的PLT与BMI等指标进行综合考虑,若捐献者BMI≥24,建议采取间隔采集模式。
基金Project supported by the National Natural Science Foundation of China(Nos.50909017,51109031, 50921001,11072053,and 51009022)the Doctoral Foundation of Ministry of Education of China(No.20100041120037)+1 种基金the Fundamental Research Funds for the Central Universities (Nos.DUT12LK52 and DUT12LK34)the Major State Basic Research Development Program of China(973 Program)(Nos.2010CB832704 and 2013CB036101)
文摘An enhanced differential transform method （EDTM）, which introduces the Pad@ technique into the standard differential transform method （DTM）, is proposed. The enhanced method is applied to the analytic treatment of the shock wave. It accelerates the convergence of the series solution and provides an exact Dower series solution.
文摘The application of flue-cured tobacco well-cellar transplanting technique un- der film and covering bowl in southwest prefecture of Guizhou province was intro- duced. And the two flue-cured tobacco well-cellar transplanting techniques were compared. Results showed that compared with flue-cured tobacco well-collar trans- planting technique under film, the technique covering bowl had the advantages of protecting ecological environment, reducing labor force and cost, resisting the bad weather and so on. This research provided theoretical foundation for the early appli- cation flue-cured tobacco well-cellar transplanting technique covering bowl in different
基金funded by the Department of Space, Government of India
文摘We present the results obtained from extensive near-infrared （IR） spectro- scopic and photometric observations of the Be/X-ray binary A0535＋262/HDE 245770 at different phases of its - 111 d orbital period. This observation campaign is part of the monitoring program of selective Be/X-ray binary systems aimed at understanding X-ray and near-IR properties at different orbital phases, especially during the perias- tron passage of the neutron star. The near-IR observations presented here were carried out using the 1.2 m telescope at the Mt. Abu IR Observatory. Though the source was relatively faint for spectroscopic observations with the 1.2 m telescope, we monitored the source closely during the 2011 February-March giant X-ray outburst to primarily investigate whether any drastic changes in the near-IR JHK spectra took place at the periastron passage. Changes of such a striking nature were expected to be detectable in our spectra. Photometric observations of the Be star show a gradual and systematic fading in the JHK light curves since the onset of the X-ray outburst, which could suggest a mild evacuation/truncation of the circumstellar disk of the Be companion. Near-IR spectroscopy of the object shows that the JHK spectra are dominated by the emission lines of hydrogen Brackett and Paschen series and HeI lines at 1.0830, 1.7002 and 2.0585 μm. The presence of all the hydrogen emission lines in the JHK spectra, along with the absence of any significant change in the continuum of the Be companion during X-ray quiescent and X-ray outburst phases, suggests that the near- IR line emitting regions of the disk are not significantly affected during the X-ray outburst.
基金Supported by the National Natural Science Foundation of China
文摘The one-meter telescope at Weihai Observatory （WHO） of Shandong University is an f/8 Cassegrain telescope. Three sets of filters are installed in a dual layer filterwheel that use Johnson-Cousins UBVRI, Sloan Digital Sky Survey u′g′r′i′z′ and Stomgren uvby. The photometric system and the CCD camera are introduced, followed by detailed analysis of their performances, and determination of the relevant parameters, including gain, readout noise, dark current and linearity of the CCD cam- era. In addition, the parameters describing the site＇s astro-climate, including typical seeing, statistics on the number of clear nights and average sky brightness, based on data gathered from Sep. 2007 to Aug. 2013, are systematically studied and reported in this work. Photometric calibrations were done using Landolt standard star obser- vations spanning eight nights, which yielded transformation coefficients, photometric precision and system throughput. The limiting magnitudes are simulated using the derived calibration parameters and classic observation conditions at WHO.
文摘The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submit-ted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as re-stricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microar-ray for hybridization. The diagnostic capability of the mi-croarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microar-ray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide mi-croarray, which can improve the positive ratio of the diagno-sis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status.
文摘As for unmanned aircraft, the knowledge of the aircraft performance is directly related with the navigation, guidance, and control system programming. Therefore, the measured data in each phase of the flight must be sufficiently precise to obtain a good characterization of aircraft.This article proposes new methods of sending information to ground, which make it possible to know the aircraft behavior accurately, and for this purpose, four contributions have been made for ALO(Avión Ligero de Observación, Spanish acronym for Light Observation Aircraft). Currently, the characterization is based on data obtained at ten samples per second, insufficient to acquire detailed knowledge of what happened during the whole flight of an aircraft. As a result of these contributions, many more samples per second of accelerations and angular velocities are obtained at the most critical moments of the flight, such as takeoff or landing. Among the improvements included are data compression techniques, providing references to locate the measured data in time and identifying labels of each parameter.
基金Supported by the National Natural Science Foundation of China (Grant No. 30370527)
文摘The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents(STOCs)in freshly isolated porcine coronary artery smooth muscle cells(ASMCs).STOCs were recorded using the perforated whole-cell patch-clamp configuration.STOCs were voltage-dependent and superimposed stochastically onto whole-cell Ca^(2+)-activated-K+(BKCa)currents.Charybdotoxin(ChTX,200 nmol/L),a selective blocker of BKCa channels,completely inhibited STOCs within 10 min.STOCs activity was greatly suppressed when extracellular Ca^(2+)concentration decreased from 1.8 mmol/L to 200 nmol/L,further removal of Ca^(2+)abolished STOCs activity.Ca^(2+)ionophore A23187(10μmol/L)increased STOCs activity significantly.Verapamil(20μmol/L)and CdCl2(200μmol/L),two kinds of organic L-type voltage-dependent Ca^(2+)channels(L-VDCCs)antagonists,had little effect on STOCs.In addition,the ryanodine receptors(RyRs)agonist caffeine(5 mmol/L)significantly activated STOCs.Application of ryanodine(50μmol/L)to block RyRs abolished STOCs,subsequent washout of ryanodine or application of caffeine failed to reproduce STOCs activity.Inhibition of inositol 1,4,5-trisphosphate receptors(IP_(3)Rs)by 2APB(40μmol/L)greatly suppressed the activity of STOCs,application of caffeine(5 mmol/L)in the presence of 2APB caused a burst of outward currents followed by inhibition of STOCs.These results suggest that STOCs in porcine coronary ASMCs are mediated by BKCa channels.Extracellular Ca^(2+)is essential for STOCs activity,while Ca^(2+)entry through L-VDCCs has little effect on STOCs.Intracellular Ca^(2+)release induced by RyRs is responsible for the regulation of STOCs,whereas IP_(3)Rs might also be involved.
文摘Defects in mitochondria are responsible for various genetic and acquired diseases.Mitochondrial transplantation,a method that involves introduction of healthy donor mitochondria into cells with dysfunctional mitochondria,could offer a novel approach to treat such diseases.Some studies have demonstrated the therapeutic benefit of mitochondrial transplantation and targeted delivery in vivo and in vitro within hepatocytes and the liver.This review discusses the issues regarding isolation and delivery of mitochondria to hepatocytes and the liver,and examines the existing literature in order to elucidate the utility and practicality of mito-chondrial transplantation in the treatment of liver disease.Studies reviewed demonstrate that mitochondrial uptake could specifically target hepatocytes,address the challenge of non-specific localization of donor mitochondria,and provide evidence of changes in liver function following injection of mitochondria into mouse and rat disease models.While potential benefits and advantages of mitochondrial trans-plantation are evident,more research is needed to determine the practicality of mitochondrial transplantation for the treatment of genetic and acquired liver diseases.
