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A review of ^(17)O isotopic labeling techniques for solid-state NMR structural studies of metal oxides in lithium-ion batteries
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作者 Xiaoli Xia Lei Zhu +2 位作者 Weiping Tang Luming Peng Junchao Chen 《Magnetic Resonance Letters》 2024年第2期46-53,共8页
Recent advances in utilizing ^(17)O isotopic labeling methods for solid-state nuclear magnetic resonance(NMR)investigations of metal oxides for lithium-ion batteries have yielded extensive insights into their structur... Recent advances in utilizing ^(17)O isotopic labeling methods for solid-state nuclear magnetic resonance(NMR)investigations of metal oxides for lithium-ion batteries have yielded extensive insights into their structural and dynamic details.Herein,we commence with a brief introduction to recent research on lithium-ion battery oxide materials studied using ^(17)O solid-state NMR spectroscopy.Then we delve into a review of ^(17)O isotopic labeling methods for tagging oxygen sites in both the bulk and surfaces of metal oxides.At last,the unresolved problems and the future research directions for advancing the ^(17)O labeling technique are discussed. 展开更多
关键词 ^(17)O solid-state NMR ^(17)O isotopic labeling methods Bulk and surfaces of metal oxides DFT calculation
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COMPARISON OF DIFFERENT STAINING METHODS FOR ANALYSING ESTERASE (EST) ISOZYME OF FUNGI AND PLANTS
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作者 池玉杰 张明 +1 位作者 程东升 许翠青 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1996年第4期24-27,共4页
EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong diff... EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher. 展开更多
关键词 FUNGI Esterase isozyme staining methods
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A Comparative Study on Four Staining Methods for Antitumor Active Fraction of Periplaneta americana
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作者 Shaohui WU Chenggui ZHANG +1 位作者 Xiumei WU Guangming LIU 《Agricultural Biotechnology》 CAS 2012年第1期61-63,共3页
[ Objective ] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel dectrophoresis (SDS-PAGE) and explore a suit- able staining method for the antitumor active fraction of P. ameri... [ Objective ] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel dectrophoresis (SDS-PAGE) and explore a suit- able staining method for the antitumor active fraction of P. americana after polyacrylamide gel electrophoresis. [ Method ] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method, potassium staining method, calcium staining method and silver staining method, on the basis, antitumor ac- tive fraction samples of P. americana were used for SDS-PAGE electrophoresis and staining. [ Result] The results showed that silver staining method could be ac- curately, quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P. amer/cana. [ Conclusion] This study laid the foundation for explo- ring the medicinal value of P. americana. 展开更多
关键词 SDS-PAGE BSA Coomassie blue staining method
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The effect of the different labeling and red cell removed methods on measuring the CD34^+cells of the cord blood
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《中国输血杂志》 CAS CSCD 2001年第S1期415-,共1页
关键词 cells of the cord blood CD The effect of the different labeling and red cell removed methods on measuring the CD34
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High-temperature thermal stability of C/C−ZrC−SiC composites via region labeling method 被引量:2
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作者 Zheng PENG Chun-mao MIAO +5 位作者 Wei SUN Yong-long XU Hai-kun CHEN Yu-feng LIU Hong-bo ZHANG Xiang XIONG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2022年第10期3349-3361,共13页
To investigate the thermal stability of ceramic-matrix composites,three kinds of C/C−ZrC−SiC composites with different Zr/Si molar ratios were synthesized by reactive melt infiltration.Employing region labeling method... To investigate the thermal stability of ceramic-matrix composites,three kinds of C/C−ZrC−SiC composites with different Zr/Si molar ratios were synthesized by reactive melt infiltration.Employing region labeling method,the high-temperature thermal stability of the composites was systematically studied by changing the temperature and holding time of thermal treatment.Results show that the mass loss rate of low Si composites has a growth trend with increasing temperature,and a crystal transformation from β-SiC toα-SiC occurs in the composites.In the calibrated area,SiC phase experiences Ostwald ripening and volume change with location migration,while ZrC phase experiences a re-sintering process with diffusion.Moreover,it is found that increasing temperature has a more obvious effect on the thermal stability than extending holding time,which is mainly attributed to the faster diffusion rate of atoms. 展开更多
关键词 thermal stability ceramic-matrix composites reactive melt infiltration high-temperature thermal treatment region labeling method
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First-Arrival Picking Method for Active Source Data with Ocean Bottom Seismometers Based on Spatial Waveform Variation Characteristics
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作者 LIU Hongwei XING Lei +3 位作者 ZHU Henghua ZHANG Jin ZHANG Jing LIU Huaishan 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期970-980,共11页
The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods fo... The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods for first-arrival picking based on sample points are characterized by theoretical errors,especially in low-sampling-frequency OBS data because the travel time of seismic waves is not an integer multiple of the sampling interval.In this paper,a first-arrival picking method that utilizes the spatial waveform variation characteristics of active source OBS data is presented.First,the distribution law of theoretical error is examined;adjacent traces exhibit variation characteristics in their waveforms.Second,a label cross-correlation superposition method for extracting highfrequency signals is presented to enhance the first-arrival picking precision.Results from synthetic and field data verify that the proposed approach is robust,successfully overcomes the limitations of low sampling frequency,and achieves precise outcomes that are comparable with those of high-sampling-frequency data. 展开更多
关键词 first-arrival picking spatial waveform variation label cross-correlation superposition method
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A RAPID STAINING METHOD SHOWING AgNOR AND DNA IN PATHOLOGICAL DIAGNOSIS
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作者 龚志锦 郑唯强 +1 位作者 詹镕洲 陶文照 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1994年第2期139-143,共5页
In order to satisfy the need of rapid clinical diagnosis, a series of experimental studies for AgNOR (Argyrophilic NORassociated proteins) and DNA (Dereyribonucleic acid) staining was compared, from which a stable sta... In order to satisfy the need of rapid clinical diagnosis, a series of experimental studies for AgNOR (Argyrophilic NORassociated proteins) and DNA (Dereyribonucleic acid) staining was compared, from which a stable staining solution was selected to show mucin residence. Using the referred tables, with reference to the corresponding relation of the staining solution with temperature and time, reliable staining results for AgNOR and DNA could be obtained.These two modified staining methods are simple, reliable and easy to operate, able to provide good contrast for pathological diagnosis. 展开更多
关键词 AgNOR staining method DNA stainingmethod.
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Introducing a Rapid and Safe Method for Myeloperoxidase Staining
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作者 Fatemeh E. Mahjoub Fahimeh Firouzjaie Karder +3 位作者 Issa Jahanzad Saghi Vaziri Ramezan Ali Sharifian Zahra Farahani 《Open Journal of Pathology》 2015年第2期38-41,共4页
Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, his... Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, histochemical stains such as myeloperoxidase stain are persistently used for better classification of leukemias. The myeloperoxidase staining is a time consuming and hazardous procedure. The present report describes a sensitive, rapid and easy method for assessment of peroxidase activity. Materials and Methods: Bone marrow aspiration slides were stained with Dako product: Code number: K3467 containing DAB chromogen (3,3-diaminobenzidine in chromogen solution) and substrate buffer (Imidasole-HCL buffer, PH 7.5 containing hydrogen peroxide and an anti microbial agent) in a rapid procedure taking only ten minutes time. The staining needs no material preparation steps. Neutrophils in the slide are taken as positive control or another normal smear was costained to be used as control. All cases were followed up with flow cytometry and cytogenetic studies. Result: The reaction product of this stain is brown and granular. Promyelocytes and myelocytes are the most strongly staining cells with positive (primary) granules. Lymphoblasts are negative. The result of classification of leukemias with this technique was in concordance with flow cytometric immunophenotyping. Discussion: Many practical techniques have been described using benzidine as an indicator for myeloperoxidase staining. Benzidine is a carcinogenic material and its usage is severely restricted in laboratory. Formerly we prepared requisite materials for myeloperoxidase staining by hazardous ways (boiling), but we decided to apply ready to use 3,3-diaminobenzidine (DAB), which is used in final step of immunohistochemistry stains. Conclusion: Use of 3,3-diaminobenzidine (DAB) is highly recommended for myeloperoxidase staining, while the result is extraordinary and fully compatible with flow cytometry and the method is safe and rapid. 展开更多
关键词 MYELOPEROXIDASE staining DAB 3 3-Diaminobenzidine Safe and RAPID method
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A NEW STAINING METHOD FOR THE MEASUREMENT OF PROTEIN USING TETRAPHENYLPORPHYRIN TETRASULFONATE(TPPS_4)
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作者 Shen Yang TONG Na LI Chemistry Department,Peking University,Beijing,100871 《Chinese Chemical Letters》 SCIE CAS CSCD 1993年第12期1079-1080,共2页
A new dye-staining method for protein assay is described.The reaction between TPPS_4 and protein molecule causes a shift in the absorption of TPPS_4 from 435 nm to 488nm.The absorbance at 488 nm is proportional to the... A new dye-staining method for protein assay is described.The reaction between TPPS_4 and protein molecule causes a shift in the absorption of TPPS_4 from 435 nm to 488nm.The absorbance at 488 nm is proportional to the concentration of protein.The range of Beer's law was 1-5 ug/ml and the Sandell's sensitivity was 0.0087 ug/cm^2. 展开更多
关键词 TPPS4 A NEW staining method FOR THE MEASUREMENT OF PROTEIN USING TETRAPHENYLPORPHYRIN TETRASULFONATE
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A simple and effective method for RBC labeling and survival studies
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《中国输血杂志》 CAS CSCD 2001年第S1期388-,共1页
关键词 RBC A simple and effective method for RBC labeling and survival studies
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THE DIVERGENT PROJECTIONS OF PERIPHERAL PROCESSES OF SUBSTANCE P-CONTAINING SPINAL GANGLIONIC NEURONS——Tri-labeled method of combining fluorescein tracing and immunocytochemistry 被引量:1
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作者 Liu Qingying Zhu Changgeng 《解剖学报》 CAS 1988年第3期336-336,共1页
The development of fluorescien double labeling method in recent years,has identified the peripheral processes of spinal ganglionic neurons project divergently to the somatic and visceral areas,but the chemical nature ... The development of fluorescien double labeling method in recent years,has identified the peripheral processes of spinal ganglionic neurons project divergently to the somatic and visceral areas,but the chemical nature of these neurons remains unknown.For this reason,we developed a tri-labeled method of combining fluorescein tracing and immunocytochemistry to clarify this problem. 展开更多
关键词 SPIN method labeled
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Syntheses of Fe-Ni Magnetic Nanoparticles and Their Applications on Biologic Labeling
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作者 戴明凤 萧仲凯 +1 位作者 李小刚 陈新泰 《过程工程学报》 CAS CSCD 北大核心 2006年第z2期249-252,共4页
In this study,we compared FeNi alloy magnetic nanoparticles(MNPs) prepared by either combustion or chemical precipitation methods.We found that the FeNi MNPs generated by combustion method have a rather high saturatio... In this study,we compared FeNi alloy magnetic nanoparticles(MNPs) prepared by either combustion or chemical precipitation methods.We found that the FeNi MNPs generated by combustion method have a rather high saturation magnetization Ms of ~180 emu/g and a coercivity field Hc of near zero.However,the alloy nanoparticles are easily aggregated and are not well dispersive such that size distribution of the nanoparticle clusters is wide and clusters are rather big(around 50~700 nm).To prepare a better quality and well dispersed Fe-Ni MNPs,we also developed a thermal reflux chemical precipitation method to synthesize FeNi3 alloy MNPs.The precursor chemicals of Fe(acac)3 and Ni(acac)2 in a molecular ratio of 1:3 reacted in octyl ether solvent at the boiling point of solvent(~300 ℃) by the thermal reflux process.The 1,2-hexadecandiol and tri-n-octylphosphine oxide(TOPO) were used as reducer and surfactant,respectively.The chemically precipitated FeNi3 MNPs are well dispersed and have well-controlled particle sizes around 10~20 nm with a very narrow size distribution(±1.2 nm).The highly monodispersive FeNi3 MNPs present good uniformity in particle shape and crystallinity on particle surfaces.The MNPs exhibit well soft magnetism with saturation magnetization of ~61 emu/g and Hc~0.The biomedically compatible FeNi MNPs which were coated with biocompatible polyethyleneimine(PEI) polymer were also synthesized.We demonstrated that the PEI coated FeNi MNPs can enter the mammalian cells in vitro and can be used as a magnetic resonance imagine(MRI) contrast agent.The results demonstrated that FeNi MNPs potentially could be applied in the biomedical field.The functionalized magnetic beads with biocompatible polymer coated on MNPs are also completed for biomedical applications. 展开更多
关键词 magnetic NANOPARTICLES FE-NI alloy chemical PRECIPITATION method BIOLOGIC labeling
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Two New Identification Methods for Encephalitozoon cuniculi on Tissue Section
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作者 Pan Yaoqian Li Ruizhen +3 位作者 Song Gaojie Zhang Zhonghua Quan Suopei Fu Yanfang 《Animal Husbandry and Feed Science》 CAS 2016年第2期75-78,共4页
[ Objective] The paper aimed to search new identification methods of Encephalitozoon cuniculi on tissue sections. [ Method] Using improved Gram staining method and methyl green pyronin staining method, the pathologica... [ Objective] The paper aimed to search new identification methods of Encephalitozoon cuniculi on tissue sections. [ Method] Using improved Gram staining method and methyl green pyronin staining method, the pathological sections of sick rabbits were stained and identified. [ Result] The pathological changes in brain tissue could be clearly observed on sections, but parasites were not examined in pathological brain tissues stained by common staining method. When the pathological section was stained by improved Gram staining method, the pathological changes in brain tissue were not ouly stained very clearly, but blue parasites were also found in brain tissues. The parasites in epithelioid cells were stained into purple ones by methyl green pyronin staining method. [ Conclusion] The im- proved Gram staining method and methyl green pyronin staining method performed good staining effects of E. cuniculi in pathological sections, which were conducive to rapid diagnosis of encephalitozoonosis in rabbit. 展开更多
关键词 Encephalitozoon cuniculi Improved Gram staining method Methyl green pyronin staining method Pathological tissue section
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Comparative Study of Z N Staining vs. Flurochrome Staining and Impact of Sample Processing on Diagnosis of Tuberculosis from Various Clinical Samples
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作者 Yogita Mistry Sangita Rajdev Summaiya Mullan 《Advances in Microbiology》 2016年第13期953-958,共6页
Background: Tuberculosis is a highly infectious disease and India has the highest burden with it. Diagnosis of tuberculosis in many countries is still dependent on microscopy. Although its sensitivity is low in compar... Background: Tuberculosis is a highly infectious disease and India has the highest burden with it. Diagnosis of tuberculosis in many countries is still dependent on microscopy. Although its sensitivity is low in comparison to culture and molecular methods, its sensitivity can still be improved by using fluorescence staining method and processing of samples by homogenization and concentration method. Material and methods: Samples were collected from all newly registered suspected cases of tuberculosis in tertiary care hospital from outward and indoor department during a period of one year. Smears were prepared for Ziehl Neelsen stain and fluorescence stain both before and after homogenization and concentration procedure by 4% NAOH-2.9% sodium citrate method and results of them were interpreted according to RNTCP criteria for grading of sputum samples. All the samples were cultured in liquid culture MGIT system (Mycobacterial Growth Indicator Tube) and results of microscopy were compared with liquid culture taken as gold standard. Data were analyzed by using SPSS software version 16. Result: 350 samples were collected during study period. Out of 350 samples, 48 samples were positive for M. tuberculosis by MGIT system. In comparison with MGIT system, sensitivity of Z N stain for detection of acid fast bacilli was 77% before decontamination procedure, which was increased up to 85.42% after decontamination and concentration process. Sensitivity of fluroscence stain was 85.42% before processing, which was increased up to 91.67% after processing of samples. Conclusion: Sensitivity of smear microscopy can be enhanced by use of fluroscence microscopy and concentration method. 展开更多
关键词 Ziehl-Neelsen staining Fluroscence staining Decontamination and Concentration method
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A revisit to staining reagents for neuronal tissues
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作者 Alexandra Rosario Ashley Howell Sanjoy K.Bhattacharya 《Annals of Eye Science》 2022年第1期51-65,共15页
In the early days of deciphering the injured neuronal tissues led to the realization that contrast is necessary to discern the parts of the recovering tissues from the damaged ones.Early attempts relied on available(a... In the early days of deciphering the injured neuronal tissues led to the realization that contrast is necessary to discern the parts of the recovering tissues from the damaged ones.Early attempts relied on available(and often naturally occurring)staining substances.Incidentally,the active ingredients of most of them were small molecules.With the advent of time,the knowledge of chemistry helped identify compounds and conditions for staining.The staining reagents were even found to enhance the visibility of the organelles.Silver impregnation identification of Golgi bodies was discovered in owl optic nerve.Staining reagents since the late 1800s were widely used across all disciplines and for nerve tissue and became a key contributor to advancement in nerve-related research.The use of these reagents provided insight into the organization of the neuronal tissues and helped distinguish nerve degeneration from regeneration.The neuronal staining reagents have played a fundamental role in the clinical research facilitating the identification of biological mechanisms underlying eye and neuropsychiatric diseases.We found a lack of systematic description of all staining reagents,whether they had been used historically or currently used.There is a lack of readily available information for optimal staining of different neuronal tissues for a given purpose.We present here a grouping of the reagents based on their target location:(I)the central nervous system(CNS),(II)the peripheral nervous system(PNS),or(III)both.The biochemical reactions of most of the staining reagents is based on acidic or basic pH and specific reaction partners such as organelle or biomolecules that exists within the given tissue type.We present here a summary of the chemical composition,optimal staining condition,use for given neuronal tissue and,where possible,historic usage.Several biomolecules such as lipids and metabolites lack specific antibodies.Despite being non-specific the reagents enhance contrast and provide corroboration about the microenvironment.In future,these reagents in combination with emerging techniques such as imaging mass spectrometry and kinetic histochemistry will validate or expand our understanding of localization of molecules within tissues or cells that are important for ophthalmology and vision science. 展开更多
关键词 staining reagent NEUROREGENERATION silver impregnation method Mallory staining hematoxylin-eosin staining
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不同类型多肉植物的花粉活力检测方法筛选及活力鉴定
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作者 杨澜 曾春雪 +2 位作者 王维泽 韦朝妹 陈之林 《种子》 北大核心 2024年第2期120-126,F0002,共8页
以3个科6个属的13种多肉植物种质资源为材料,对比亚历山大染色法和碘-碘化钾(I2-KI)染色法染色后花粉活力的差异,筛选适宜不同类型多肉植物花粉活力的测定方法。结果表明,两种染色法对不同科属的多肉植物花粉染色结果差异极显著,I2-KI... 以3个科6个属的13种多肉植物种质资源为材料,对比亚历山大染色法和碘-碘化钾(I2-KI)染色法染色后花粉活力的差异,筛选适宜不同类型多肉植物花粉活力的测定方法。结果表明,两种染色法对不同科属的多肉植物花粉染色结果差异极显著,I2-KI染色法检测的花粉活力平均值显著低于亚历山大染色法;种质资源的花粉活力普遍高于杂交组合,聚类分析结合差异显著性分析将26种目标种质资源及杂交组合的花粉活力划分为5个等级;显微镜下花粉形状呈现圆形、方形、椭圆形和不规则形,且同一品种存在一种到多种的花粉萌发孔形态。 展开更多
关键词 多肉植物 花粉活力 染色法对比 活力等级划分
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72例头癣患儿真菌荧光染色与皮肤镜特征分析
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作者 马欣 张艳丽 《中国真菌学杂志》 CSCD 2024年第4期348-351,共4页
目的研究拟通过真菌荧光染色镜检技术结合皮肤镜分析不同临床类型头癣的特点,为临床提供准确、快速诊断头癣的依据。方法收集72例头癣患儿,使用荧光染色法结合皮肤镜观察头癣病发的主要特征;观察随访患儿治疗后荧光染色法及皮肤镜特殊... 目的研究拟通过真菌荧光染色镜检技术结合皮肤镜分析不同临床类型头癣的特点,为临床提供准确、快速诊断头癣的依据。方法收集72例头癣患儿,使用荧光染色法结合皮肤镜观察头癣病发的主要特征;观察随访患儿治疗后荧光染色法及皮肤镜特殊表征是否同时转阴。结果根据临床特征,72例头癣患儿以白癣为主,占58.33%(42/72);荧光染色法阳性率100%,发外孢子占比最高,为62.50%(45/72);皮肤镜诊断的阳性率100%,皮肤镜检查白癣以断发(90.48%)为主、脓癣以脓疱(100%)为主,以逗号发/螺旋发/条码样发为特殊的表征;随访率为59.72%(43/72)。结论真菌荧光染色法结合皮肤镜特征能对头癣疾病及其临床类型做出快速且准确的诊断。 展开更多
关键词 头癣 荧光染色法 皮肤镜
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样本直接涂片法与液基夹层杯涂片法查找抗酸杆菌的效果比较
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作者 罗少珍 刘欣 +2 位作者 张志坚 黄业伦 刘志辉 《国际医药卫生导报》 2024年第6期1005-1008,共4页
目的探讨液基夹层杯涂片法查找抗酸杆菌检验方法的临床应用价值。方法从广州市胸科医院医学检验科LIS系统中收集、整理2021年1月至7月痰液与支气管冲洗液主要送检临床样本的涂片染色检查和分枝杆菌培养结果,对同一患者时间间隔不超过1... 目的探讨液基夹层杯涂片法查找抗酸杆菌检验方法的临床应用价值。方法从广州市胸科医院医学检验科LIS系统中收集、整理2021年1月至7月痰液与支气管冲洗液主要送检临床样本的涂片染色检查和分枝杆菌培养结果,对同一患者时间间隔不超过1周的直接涂片与液基夹层杯涂片查找抗酸杆菌检验的结果进行回顾性分析。结果在241份痰液样本、178份支气管冲洗液样本和全部419份样本的检测中,直接涂片法、液基夹层杯涂片法的阳性率分别为15.35%(37/241)和13.69%(33/241)、15.17%(27/178)和12.92%(23/178)、15.27%(64/419)和13.37%(56/419)。在同期分枝杆菌培养结果阳性的65份痰液样本、50份支气管冲洗液样本和115份全部阳性样本中,直接涂片法、液基夹层杯涂片法的阳性率分别为56.52%(65/115)和50.78%(65/128)、54.34%(50/92)和45.88%(50/109)、55.56%(115/207)和48.73%(115/236)。在241份痰液样本、178份支气管冲洗液样本检测中,直接涂片阳性且液基夹层杯涂片阳性、直接涂片阳性且液基夹层杯涂片阴性、直接涂片阴性且液基夹层杯涂片阳性、直接涂片阴性且液基夹层杯涂片阴性的样本占比分别为12.03%(29/241)、3.32%(8/241)、1.66%(4/241)、82.99%(200/241)和8.99%(16/178)、6.18%(11/178)、3.93%(7/178)、80.90%(144/178)。结论液基夹层杯涂片法查找抗酸杆菌检验方法总体阳性检出率略低于直接涂片检测法,但可以发现相当比例的直接涂片法阴性的病原学阳性结核病患者,尤其是在支气管冲洗液检测中,具有一定的临床应用价值。 展开更多
关键词 抗酸杆菌 抗酸染色 液基夹层杯涂片法 直接涂片法
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碱性副品红染色法检测木质部导管栓塞技术改进
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作者 张玲玲 《浙江林业科技》 2024年第3期74-80,共7页
染色法是测量导管长度、直径、导管分布以及栓塞脆弱性的重要方法。在测量导管栓塞时,染色压力和时间条件将极大地影响实验结果的准确性。为准确利用染色法测定刺槐Robinia pseudoacacia导管栓塞状况,本研究通过使用充气组(人为栓塞100%... 染色法是测量导管长度、直径、导管分布以及栓塞脆弱性的重要方法。在测量导管栓塞时,染色压力和时间条件将极大地影响实验结果的准确性。为准确利用染色法测定刺槐Robinia pseudoacacia导管栓塞状况,本研究通过使用充气组(人为栓塞100%)以及冲洗组(完全去除栓塞)对碱性副品红染色法进行了压力、时间条件的改良。结果表明,当压力在0.04kpa,染色时间60min时,实验结果最为准确可靠。在此压力下染色过程不会冲开栓塞导管,配合60 min的染色时间可对未栓塞导管进行充分染色。另外,本研究通过诱导组,利用离心机法对枝条诱导不同程度的栓塞与染色法进行了对比验证,结果表明在该染色条件下,可以很好地反映茎段的栓塞状况,保证了实验结果的准确性。在后续试验中,使用碱性副品红染色法研究木质部栓塞时可以沿用此压力及时间条件。 展开更多
关键词 木质部 刺槐 栓塞 染色法
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酶标仪法测定离心泵泵头表面肝素涂层生物活性的研究
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作者 徐苏华 刘婧群 +1 位作者 许朝生 黄敏菊 《中国体外循环杂志》 2024年第5期415-419,共5页
目的 建立酶标仪法测定离心泵泵头表面肝素的生物活性。方法 由抗凝血酶(AT)饱和的肝素化表面失活的凝血酶量来量化肝素生物活性:肝素表面与过量的血浆蛋白AT结合,形成AT饱和的肝素化表面(过量的AT通过清洗程序去除),上述肝素化表面又... 目的 建立酶标仪法测定离心泵泵头表面肝素的生物活性。方法 由抗凝血酶(AT)饱和的肝素化表面失活的凝血酶量来量化肝素生物活性:肝素表面与过量的血浆蛋白AT结合,形成AT饱和的肝素化表面(过量的AT通过清洗程序去除),上述肝素化表面又与过量凝血酶Ⅱa结合,残留的凝血酶Ⅱa与显色底物反应,凝血酶肽键H-D-Phe-Pip-Arg-pNa被裂解,释放出生色团,其可以在405 nm下进行测试并定量。结果 应用酶标仪上波长405 nm时的动力学吸光度评估生物活性,生物活性测定表明:对照品(无涂层的离心泵泵头)的结果为-0.01 IU/cm^(2)(≤0.05 IU/cm^(2)),所有3种供试品的结果为0.29 IU/cm^(2)、0.40 IU/cm^(2)和0.32 IU/cm^(2)(均≥0.1 IU/cm^(2)),符合生物活性试验的有效性标准,对照品和供试品的生物活性均被视为通过。结论 通过酶标仪定量测试凝血酶肽键裂解释放生色团的含量,可得到与肝素涂层反应的Ⅱa的量,进一步反推AT的量,最终计算出肝素涂层的生物活性。该方法操作简便,一次可以测定多份样本,检测速度快;具有需要的样本量少、重复性好的特点,适用于对离心泵泵头表面肝素涂层的生物活性定量检测。 展开更多
关键词 酶标仪法 离心泵泵头 肝素生物活性 抗凝血酶 凝血酶 体外膜氧合
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