<div style="text-align:justify;"> Bud stems arisen from in vitro cultures of A. scoparia were encapsulated in calcium alginate pieces for short term stockpile and germplasm interchange. The maximum fre...<div style="text-align:justify;"> Bud stems arisen from in vitro cultures of A. scoparia were encapsulated in calcium alginate pieces for short term stockpile and germplasm interchange. The maximum frequency (88.96%) of conversion of encapsulated nodal segments into plantlets and the highest node number (7.20) was performed on a murashige and Skoog’s medium (MS medium) containing 2.2 μM banzyl adenine (BA). The highest length of re-growing shoots was achieved when MS medium was supplied with 2.2 μM BA and 0.5 μM NAA. However, the number of shoots produced was higher (5.30 and 5.10) on MS medium supplemented with 2.2 μM BA and 0.5 μM NAA and MS medium with 2.2 μM BA, respectively than on the hormone-free media. Treatment with 19.6 μM IBA resulted in the highest conversion of encapsulated nodal segments into plantlets. The frequency of conversion (89.6% - 88.6%) was retained at 25?C for up to 2 weeks without significant change. The highest frequencies (61.1%) of plantlet formation from encapsulated nodal segments were obtained by transferring synthetic seeds onto peat mass and perlite (2:1) (v/v) mixture substrate. When transplanted into the peat mass and perlite (2:1) (v/v) mixture, these plantlets showed greater plantlet high, leaf number, shoot number and root number per plantlet than those of the other substrates. The synthetic seed technology offered a promising way for short term storage without refrigerating, germplasm conservation exchange for improvement and an alternative clonal propagation method for this endangered genotype of <em>A. scoparia</em>. </div>展开更多
The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and the...The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.展开更多
文摘<div style="text-align:justify;"> Bud stems arisen from in vitro cultures of A. scoparia were encapsulated in calcium alginate pieces for short term stockpile and germplasm interchange. The maximum frequency (88.96%) of conversion of encapsulated nodal segments into plantlets and the highest node number (7.20) was performed on a murashige and Skoog’s medium (MS medium) containing 2.2 μM banzyl adenine (BA). The highest length of re-growing shoots was achieved when MS medium was supplied with 2.2 μM BA and 0.5 μM NAA. However, the number of shoots produced was higher (5.30 and 5.10) on MS medium supplemented with 2.2 μM BA and 0.5 μM NAA and MS medium with 2.2 μM BA, respectively than on the hormone-free media. Treatment with 19.6 μM IBA resulted in the highest conversion of encapsulated nodal segments into plantlets. The frequency of conversion (89.6% - 88.6%) was retained at 25?C for up to 2 weeks without significant change. The highest frequencies (61.1%) of plantlet formation from encapsulated nodal segments were obtained by transferring synthetic seeds onto peat mass and perlite (2:1) (v/v) mixture substrate. When transplanted into the peat mass and perlite (2:1) (v/v) mixture, these plantlets showed greater plantlet high, leaf number, shoot number and root number per plantlet than those of the other substrates. The synthetic seed technology offered a promising way for short term storage without refrigerating, germplasm conservation exchange for improvement and an alternative clonal propagation method for this endangered genotype of <em>A. scoparia</em>. </div>
文摘The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.
