[Objectives] To establish the process flow of preparation of Wufang Babu Poultice and the identification method of thin layer chromatography (TLC). [Methods] For the forming process of Wufang Babu Poultice, the prepar...[Objectives] To establish the process flow of preparation of Wufang Babu Poultice and the identification method of thin layer chromatography (TLC). [Methods] For the forming process of Wufang Babu Poultice, the preparation method of mixed pharmaceutical powder with suitable pharmaceutical excipients was adopted. Qualitative identification of medicinal materials in Wufang Babu Poultice (Strychni Semen, Rhei Radix Et Rhizoma, Dipsaci Radix, and Angelicae Sinensis Radix) was carried out by TLC. [Results] Mixed pharmaceutical powder mixed with glycerol, gelatin and other pharmaceutical excipients can be prepared for forming. The test solution chromatography of each medicinal material (Strychni Semen, Rhei Radix et Rhizoma, Dipsaci Radix, Angelicae Sinensis Radix) showed pigment spots of the same color at the same position as its corresponding control medicinal materials and reference chromatography, and the display was clear. [Conclusions] The preparation process is simple and feasible, and can be used as the forming process of Wufang Babu Poultice. The TLC determination method is simple to operate, has good specificity, and has no effect on negative results, and can be used for identification of Wufang Babu Poultice.展开更多
[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edib...[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.展开更多
基金Supported by Key R&D Plan of Guangxi Zhuang Autonomous Region(Guike AB20297010).
文摘[Objectives] To establish the process flow of preparation of Wufang Babu Poultice and the identification method of thin layer chromatography (TLC). [Methods] For the forming process of Wufang Babu Poultice, the preparation method of mixed pharmaceutical powder with suitable pharmaceutical excipients was adopted. Qualitative identification of medicinal materials in Wufang Babu Poultice (Strychni Semen, Rhei Radix Et Rhizoma, Dipsaci Radix, and Angelicae Sinensis Radix) was carried out by TLC. [Results] Mixed pharmaceutical powder mixed with glycerol, gelatin and other pharmaceutical excipients can be prepared for forming. The test solution chromatography of each medicinal material (Strychni Semen, Rhei Radix et Rhizoma, Dipsaci Radix, Angelicae Sinensis Radix) showed pigment spots of the same color at the same position as its corresponding control medicinal materials and reference chromatography, and the display was clear. [Conclusions] The preparation process is simple and feasible, and can be used as the forming process of Wufang Babu Poultice. The TLC determination method is simple to operate, has good specificity, and has no effect on negative results, and can be used for identification of Wufang Babu Poultice.
基金Scientific Research Project for School-level Teachers of Sichuan College of Traditional Chinese Medicine in 2023(23ZRYB08)Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory Open Fund Project of Southwest Minzu University(QTPEM2305).
文摘[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.
