An affinity adsorbent, benzamidine Sepharose 4B, was used to separate and purify thrombin like enzymes. The p aminobenzamidine as a specific ligand was coupled to the matrix-Sepharose 4B. The recombinant thrombin like...An affinity adsorbent, benzamidine Sepharose 4B, was used to separate and purify thrombin like enzymes. The p aminobenzamidine as a specific ligand was coupled to the matrix-Sepharose 4B. The recombinant thrombin like enzyme-defibrase was used as a model in order to evaluate the efficiency of this biospecific affinity adsorbent. The homogeneity of the enzyme preparation was comfirmed as one band on sodium dodecyl sulfate polyacrylamide gel electrophoresis.展开更多
将纯化得到的蛇毒凝血酶(TLE_3、TLE_4),经家兔体内实验表明,2—3凝血酶单位/kg体重剂量能显著地使家兔全血凝血时间缩短1/3—1/2。药后1小时即有促凝作用,以2—4小时凝血(止血)效应最强,12小时已消失。与Holleman, W. H.等自美洲矛头...将纯化得到的蛇毒凝血酶(TLE_3、TLE_4),经家兔体内实验表明,2—3凝血酶单位/kg体重剂量能显著地使家兔全血凝血时间缩短1/3—1/2。药后1小时即有促凝作用,以2—4小时凝血(止血)效应最强,12小时已消失。与Holleman, W. H.等自美洲矛头蝮蛇毒中得到的蛇毒凝血酶(Hemocoagulase)相似。经家兔及家犬实验性创伤止血实验表明,对创伤出血有止血作用。展开更多
文摘An affinity adsorbent, benzamidine Sepharose 4B, was used to separate and purify thrombin like enzymes. The p aminobenzamidine as a specific ligand was coupled to the matrix-Sepharose 4B. The recombinant thrombin like enzyme-defibrase was used as a model in order to evaluate the efficiency of this biospecific affinity adsorbent. The homogeneity of the enzyme preparation was comfirmed as one band on sodium dodecyl sulfate polyacrylamide gel electrophoresis.
文摘将纯化得到的蛇毒凝血酶(TLE_3、TLE_4),经家兔体内实验表明,2—3凝血酶单位/kg体重剂量能显著地使家兔全血凝血时间缩短1/3—1/2。药后1小时即有促凝作用,以2—4小时凝血(止血)效应最强,12小时已消失。与Holleman, W. H.等自美洲矛头蝮蛇毒中得到的蛇毒凝血酶(Hemocoagulase)相似。经家兔及家犬实验性创伤止血实验表明,对创伤出血有止血作用。
