Based on the system of electric power supply for flexible manufacturing systems (FMS), a study has been carried out on the intelligent safety examination, monitoring and maintenance of its running environment. On the ...Based on the system of electric power supply for flexible manufacturing systems (FMS), a study has been carried out on the intelligent safety examination, monitoring and maintenance of its running environment. On the basis of the specific feature of the power supply network of an FMS, real time monitoring system of the power supply network and the fault diagnostic expert system for the power equipment have been designed. This system can diagnose not only definite fault phenomena, but also fuzzy, uncertain fault phenomena as well. Fault diagnostic knowledge base for the power equipment has been founded hierarchy architecture model and the method of fault tree analysis. Feasibility of this system has been proved by computer simulation.展开更多
Radar slope monitoring is now widely used across the world, for example, the slope stability radar(SSR)and the movement and surveying radar(MSR) are currently in use in many mines around the world.However, to fully re...Radar slope monitoring is now widely used across the world, for example, the slope stability radar(SSR)and the movement and surveying radar(MSR) are currently in use in many mines around the world.However, to fully realize the effectiveness of this radar in notifying mine personnel of an impending slope failure, a method that can confidently predict the time of failure is necessary. The model developed in this study is based on the inverse velocity method pioneered by Fukuzono in 1985. The model named the slope failure prediction model(SFPM) was validated with the displacement data from two slope failures monitored with the MSR. The model was found to be very effective in predicting the time to failure while providing adequate evacuation time once the progressive displacement stage is reached.展开更多
In the context of this work,a prototype hybrid photoacoustic(PA)and optical system for the on-line monitoring of laser cleaning procedures is presented.The developed apparatus has enabled the detection of MHz frequenc...In the context of this work,a prototype hybrid photoacoustic(PA)and optical system for the on-line monitoring of laser cleaning procedures is presented.The developed apparatus has enabled the detection of MHz frequency range acoustic waves generated during the laser ablation process.The intrinsically generated PA signals combined with high resolution optical images provide the opportunity to follow the cleaning process accurately and in real time.Technical mock-ups have been used to demonstrate the potential of this novel technique with emphasis given to applications that refer to the restoration of Cultural Heritage(CH)surfaces.Towards this purpose,the real time monitoring of the laser assisted removal of unwanted encrustation from stonework has been achieved using IR and UV wavelengths.This novel approach has allowed for the precise determination of the critical number of laser pulses required for the elimination of the encrustation layer,while highlighting the dominant ablation mechanisms according to the irradiation wavelength.The promising results obtained using the prototype hybrid PA and optical system can open up new perspectives in the monitoring of laser cleaning interventions,promoting an improved restoration outcome.展开更多
An introduction is made to the composition, design method and engineering application of a remote real time monitoring system of power quality in substations based on internet. With virtual instrument and network tec...An introduction is made to the composition, design method and engineering application of a remote real time monitoring system of power quality in substations based on internet. With virtual instrument and network technique adopted, this system is characterized by good real time property, high reliability, plentiful functions, and so on. It also can be used to monitor the load of a substation, such as electric locomotives.展开更多
To better understand the mechanical properties of marble at Jinping II hydropower station, this paper examines the changes of brittle rocks in excavation damaged zones(EDZs) before and after excavation of tunnel with ...To better understand the mechanical properties of marble at Jinping II hydropower station, this paper examines the changes of brittle rocks in excavation damaged zones(EDZs) before and after excavation of tunnel with the tunnel boring machine(TBM). The paper attempts to employ the acoustic emission(AE) to study the AE characteristics and distribution of rockburst before and after TBM-excavated tunnel. It is known that the headrace tunnel #2, excavated by the drill-and-blast(D&B) method, is ahead of the headrace tunnel #3 that is excavated by TBM method. The experimental sub-tunnel #2–1, about 2000 m in depth and 13 m in diameter, between the two tunnels is scheduled. In the experimental sub-tunnel #2–1, a large number of experimental boreholes are arranged, and AE sensors are installed within 10 m apart from the wall of the headrace tunnel #3. By tracking the microseismic signals in rocks, the location, frequency, quantity, scope and intensity of the microseismic signals are basically identifed. It is observed that the AE signals mainly occur within 5 m around the rock wall, basically lasting for one day before tunnel excavation and a week after excavation. Monitoring results indicate that the rockburst signals are closely related to rock stress adjustment. The rock structure has a rapid self-adjustment capacity before and after a certain period of time during tunneling. The variations of rock stresses would last for a long time before reaching a fnal steady state. Based on this, the site-specifc support parameters for the deep tunnels can be accordingly optimized.展开更多
Real time rainfall events monitoring is very important for a large number of reasons: Civil Protection, hydrogeological risk management, hydroelectric power purposes, road and traffic regulation, and tourism. Efficien...Real time rainfall events monitoring is very important for a large number of reasons: Civil Protection, hydrogeological risk management, hydroelectric power purposes, road and traffic regulation, and tourism. Efficient monitoring operations need continuous, high-resolution and large-coverage data. To monitor and observe extreme rainfall events, often much localized over small basins of interest, and that could frequently causing flash floods, an unrealistic extremely dense rain gauge network should be needed. On the other hand, common large C-band or S-band long range radars do not provide the necessary spatial and temporal resolution. Simple short-range X-band mini weather radar can be a valid compromise solution. The present work shows how a single polarization, non-Doppler and non-coherent, simple and low cost X-band radar allowed monitoring three very intense rainfall events occurred near Turin during July 2014. The events, which caused damages and floods, are detected and monitored in real time with a sample rate of 1 minute and a radial spatial resolution of 60 m, thus allowing to describe the intensity of the precipitation on each small portion of territory. This information could be very useful if used by authorities in charge of Civil Protection in order to avoid inconvenience to people and to monitor dangerous situations.展开更多
Landslide risk is increasing in many parts of the world due to growth of population and infrastructures. Therefore, an effort has to be made in developing new and cheap sensors for areas susceptible of landslides to c...Landslide risk is increasing in many parts of the world due to growth of population and infrastructures. Therefore, an effort has to be made in developing new and cheap sensors for areas susceptible of landslides to continuously control the slope behaviour, until approaching failure conditions. The paper reported experimental data from smallscale physical models about the performance of Time Domain Reflectometry(TDR) and optical fibres, which act as the indicators of the incoming failure of slopes covered by unsaturated granular soils. Obtained results appear encouraging, since both sensors provide continuous information about the state of the slope, in terms of water content profiles and ongoing deformations, induced by rainwater infiltration, even immediately before the triggering of a fast landslide.展开更多
Based on the analysis of different requirements of energy management center construction and the data acquisition of various industries in wide area network, as well as the practices of real-lime online system, the pa...Based on the analysis of different requirements of energy management center construction and the data acquisition of various industries in wide area network, as well as the practices of real-lime online system, the paper puts forward the construction scheme of regional energy management center （REMC） which can achieve real time online monitoring of organizations＇ energy consumption via data collection, and also proposes the design idea of energy data acquisition based on national standards.展开更多
Bamboo Winding Composite Pipes(BWCPs)have many advantages such as good mechanical properties,low cost,low carbon,environmental friendliness,thermal insulation and easy installation et al.At present,BWCP is widely used...Bamboo Winding Composite Pipes(BWCPs)have many advantages such as good mechanical properties,low cost,low carbon,environmental friendliness,thermal insulation and easy installation et al.At present,BWCP is widely used in urban water supply,drainage,communication cable protection,farmland irrigation etc.It is obvious that the Bamboo Winding Composite materials have good application prospects in the field of utility tunnels.However,considering that the size of utility tunnels is much larger than that of normal pipelines,the wide application of Bamboo Composite Utility Tunnels(BCUTs)is still a challenge.In this paper,the force performance of BCUT in Datong Shanxi Province,was studied using two methods:real time monitoring and numerical simulation.Wireless sensor networks were used for monitoring,and the real time monitoring data of horizontal convergence and strains under the condition of backfill were obtained.The monitoring data indicated that the horizontal convergence can meet the requirement of relevant technical standards.A three-dimensional numerical model of the utility tunnel was established using FEM software.The influence of soil parameters on the deformation and strain of the BCUT was studied using the numerical method.The simulation results were compared with real-time monitoring data.The soil parameters K_(0) and k_(sh) have significant effects on the deformation,stress and strain of the utility tunnel.This kind of utility tunnel is recommended to use in areas with relatively good geological conditions.展开更多
Rapidly acquiring and real-time transmitting crop water requirement information constitute the basis for achieving intelligent diagnosis and precision irrigation.In order to collect and transmit crop water requirement...Rapidly acquiring and real-time transmitting crop water requirement information constitute the basis for achieving intelligent diagnosis and precision irrigation.In order to collect and transmit crop water requirement information at real time,a new microcontroller-based real-time remote monitoring system was designed,including system hardware design,software and anti-jamming design.The system achieved the functions including clock reading,information configuration,LCD display,keyboard control,data sending and receiving,multi-channel information acquisition,conversion and storage.Laboratory and field tests showed that the system can achieve data acquisition and real-time display of the crop water requirement information.Unlike the current weather station,the system collects crop water information,meteorological factors and soil parameters at the same time.It has a high level of stability and acquisition accuracy,and can meet the requirements for real-time remote monitoring of the crop water requirement information for irrigation decision-making.展开更多
Large dams are complex structures with nonlinear dynamic behavior.Engineers often are forced to assess dam safety based on the available incomplete data,which is extremely difficult.This important problem can be solve...Large dams are complex structures with nonlinear dynamic behavior.Engineers often are forced to assess dam safety based on the available incomplete data,which is extremely difficult.This important problem can be solved with the modern theory of complex systems.It is possible to derive characteristics of the whole unknown dynamics of a structure using few data sets of certain carefully selected representative parameter(s).By means of high quality continuous records of some geotechnical characteristic(s)of a dam and modern methods of time series linear/nonlinear analysis the main dynamical features of the entire,unknown process(here—dam deformation)can be analyzed.We created the cost-effective Monitoring Telemetric System for Dam Diagnostics(DAMWATCH),which consists of sensors(tiltmeters),terminal and central controllers connected by the GSM/GPRS Modem to the diagnostic center.The tilt data recorded for varying reservoir level are compared with static design model of dam deformations computed by a finite element method(FEM)for the dam-reservoir-foundation system.Besides,recently developed linear/nonlinear data analysis and prediction schemes may help to quantify fine dynamical features of the dam behavior.The software package DAMTOOL has been developed for this purpose.The differences between measured and theoretically predicted response parameters of the dam may signal abnormal behavior of the object.The data obtained already by testing of the DAMWATCH/DAMTOOL system during operation of the high Enguri arc dam and reservoir(Georgia)show interesting long-term and short-term patterns of tilts in the dam body,which can be used for dam diagnostics.The proposed real-time telemetric monitoring(DAMWATCH)complex and linear/nonlinear dynamical analysis system(DAMTOOL)are unique.展开更多
We propose a technique for chromatic dispersion monitoring based on optical time domain level monitoring. Experimental and simulation results show that the technique is effective for the monitoring of dispersion in 42...We propose a technique for chromatic dispersion monitoring based on optical time domain level monitoring. Experimental and simulation results show that the technique is effective for the monitoring of dispersion in 42.7-Gbps CS-RZ signals for dynamic dispersion compensation.展开更多
Compared to small molecule process analytical technology （PAT） applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell...Compared to small molecule process analytical technology （PAT） applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell culture process is essential to establish an appropriate process control strategy for biotechnology product PAT applications. Inline spectroscopic techniques for real time monitoring of bioreactor cell culture process have the distinct potential to develop PAT approaches in manufac- turing biotechnology drug products. However, the use of inline Fourier transform infrared （FTIR） spectroscopic techniques for bioreactor cell culture process monitoring has not been reported. In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell culture fluid biomolecular dynamic model. The technical feasibility of using FTIR Spectroscopy for real time tracking and monitoring four key cell culture metabolites （including glucose, glutamine, lactate, and ammonia） and protein yield at increasing levels of complexity （simple binary system, fully formulated media, actual bioreactor cell culture process） was evaluated via a stepwise approach. The FTIR fingerprints of the key metabolites were identified. The multivariate partial least squares （PLS） calibration models were established to correlate the process FTIR spectra with the concentrations of key metabolites and protein yield of in-process samples, either individually for each metabolite and protein or globally for all four metabolites simultaneously. Applying the 2＇ld derivative pre-processing algorithm to the FTIR spectra helps to reduce the number of PLS latent variables needed significantly and thus simplify the interpretation of the PLS models. The validated PLS models show promise in predicting the concentration profiles of glucose, glutamine, lactate, and ammonia and protein yield over the course of the bioreactor cell culture process. Therefore, this work demonstrated the technical feasibility of real time monitoring of the bioreactor cell culture process via FTIR spectroscopy. Its implications for enabling cell culture PAT were discussed.展开更多
The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neur...The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte- neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neu- ronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here,we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.展开更多
Normalized Difference Vegetation Index(NDVI)has been found to have good correlations with many physical properties of soybean surfaces.Due to the factors of air temperature,humidity,solar radiation,soil moisture,etc.,...Normalized Difference Vegetation Index(NDVI)has been found to have good correlations with many physical properties of soybean surfaces.Due to the factors of air temperature,humidity,solar radiation,soil moisture,etc.,NDVI of soybean varies dynamically in a day.The establishment of the soybean NDVI prediction model at different times in a day can effectively modify this variation.The soybean NDVI values are continuously monitored in hours during soybean seeding,flowering&podding and maturating stages by way of Green Seeker.Results show that the trend of NDVI change every day in the three stages is taken on as a reverse parabola.The NDVI value reaches to the maximum at 8 am or 9 am and decreases to its minimum at 2 pm before a moderate rise.A model for intraday and long-term NDVI change for soybean is built.The test of the model with independent data indicates that the precision meets the demands,with the root mean square error(RMSE)of each day being 3.95,5.45 and 2.86 for the seeding stage,the bean podding stage and the maturation period,respectively.The prediction RMSEs of the soybean NDVI model for soybeans of the three stages for the fifth day are 5.75,2.65 and 5.51,respectively and the prediction RMSEs for the sixth day are 9.74,2.82 and 14.04,respectively according to the data from the first four days.展开更多
文摘Based on the system of electric power supply for flexible manufacturing systems (FMS), a study has been carried out on the intelligent safety examination, monitoring and maintenance of its running environment. On the basis of the specific feature of the power supply network of an FMS, real time monitoring system of the power supply network and the fault diagnostic expert system for the power equipment have been designed. This system can diagnose not only definite fault phenomena, but also fuzzy, uncertain fault phenomena as well. Fault diagnostic knowledge base for the power equipment has been founded hierarchy architecture model and the method of fault tree analysis. Feasibility of this system has been proved by computer simulation.
基金supported by the Centennial Trust Fund, School of Mining Engineering, University of the Witwatersrand, South Africa
文摘Radar slope monitoring is now widely used across the world, for example, the slope stability radar(SSR)and the movement and surveying radar(MSR) are currently in use in many mines around the world.However, to fully realize the effectiveness of this radar in notifying mine personnel of an impending slope failure, a method that can confidently predict the time of failure is necessary. The model developed in this study is based on the inverse velocity method pioneered by Fukuzono in 1985. The model named the slope failure prediction model(SFPM) was validated with the displacement data from two slope failures monitored with the MSR. The model was found to be very effective in predicting the time to failure while providing adequate evacuation time once the progressive displacement stage is reached.
文摘In the context of this work,a prototype hybrid photoacoustic(PA)and optical system for the on-line monitoring of laser cleaning procedures is presented.The developed apparatus has enabled the detection of MHz frequency range acoustic waves generated during the laser ablation process.The intrinsically generated PA signals combined with high resolution optical images provide the opportunity to follow the cleaning process accurately and in real time.Technical mock-ups have been used to demonstrate the potential of this novel technique with emphasis given to applications that refer to the restoration of Cultural Heritage(CH)surfaces.Towards this purpose,the real time monitoring of the laser assisted removal of unwanted encrustation from stonework has been achieved using IR and UV wavelengths.This novel approach has allowed for the precise determination of the critical number of laser pulses required for the elimination of the encrustation layer,while highlighting the dominant ablation mechanisms according to the irradiation wavelength.The promising results obtained using the prototype hybrid PA and optical system can open up new perspectives in the monitoring of laser cleaning interventions,promoting an improved restoration outcome.
文摘An introduction is made to the composition, design method and engineering application of a remote real time monitoring system of power quality in substations based on internet. With virtual instrument and network technique adopted, this system is characterized by good real time property, high reliability, plentiful functions, and so on. It also can be used to monitor the load of a substation, such as electric locomotives.
文摘To better understand the mechanical properties of marble at Jinping II hydropower station, this paper examines the changes of brittle rocks in excavation damaged zones(EDZs) before and after excavation of tunnel with the tunnel boring machine(TBM). The paper attempts to employ the acoustic emission(AE) to study the AE characteristics and distribution of rockburst before and after TBM-excavated tunnel. It is known that the headrace tunnel #2, excavated by the drill-and-blast(D&B) method, is ahead of the headrace tunnel #3 that is excavated by TBM method. The experimental sub-tunnel #2–1, about 2000 m in depth and 13 m in diameter, between the two tunnels is scheduled. In the experimental sub-tunnel #2–1, a large number of experimental boreholes are arranged, and AE sensors are installed within 10 m apart from the wall of the headrace tunnel #3. By tracking the microseismic signals in rocks, the location, frequency, quantity, scope and intensity of the microseismic signals are basically identifed. It is observed that the AE signals mainly occur within 5 m around the rock wall, basically lasting for one day before tunnel excavation and a week after excavation. Monitoring results indicate that the rockburst signals are closely related to rock stress adjustment. The rock structure has a rapid self-adjustment capacity before and after a certain period of time during tunneling. The variations of rock stresses would last for a long time before reaching a fnal steady state. Based on this, the site-specifc support parameters for the deep tunnels can be accordingly optimized.
文摘Real time rainfall events monitoring is very important for a large number of reasons: Civil Protection, hydrogeological risk management, hydroelectric power purposes, road and traffic regulation, and tourism. Efficient monitoring operations need continuous, high-resolution and large-coverage data. To monitor and observe extreme rainfall events, often much localized over small basins of interest, and that could frequently causing flash floods, an unrealistic extremely dense rain gauge network should be needed. On the other hand, common large C-band or S-band long range radars do not provide the necessary spatial and temporal resolution. Simple short-range X-band mini weather radar can be a valid compromise solution. The present work shows how a single polarization, non-Doppler and non-coherent, simple and low cost X-band radar allowed monitoring three very intense rainfall events occurred near Turin during July 2014. The events, which caused damages and floods, are detected and monitored in real time with a sample rate of 1 minute and a radial spatial resolution of 60 m, thus allowing to describe the intensity of the precipitation on each small portion of territory. This information could be very useful if used by authorities in charge of Civil Protection in order to avoid inconvenience to people and to monitor dangerous situations.
基金partially supported by the project Safe Land "Living with landslide risk in Europe: Assessment, effects of global change, and risk management strategies" under Grant No. 226479 (7th Framework Programme)
文摘Landslide risk is increasing in many parts of the world due to growth of population and infrastructures. Therefore, an effort has to be made in developing new and cheap sensors for areas susceptible of landslides to continuously control the slope behaviour, until approaching failure conditions. The paper reported experimental data from smallscale physical models about the performance of Time Domain Reflectometry(TDR) and optical fibres, which act as the indicators of the incoming failure of slopes covered by unsaturated granular soils. Obtained results appear encouraging, since both sensors provide continuous information about the state of the slope, in terms of water content profiles and ongoing deformations, induced by rainwater infiltration, even immediately before the triggering of a fast landslide.
文摘Based on the analysis of different requirements of energy management center construction and the data acquisition of various industries in wide area network, as well as the practices of real-lime online system, the paper puts forward the construction scheme of regional energy management center （REMC） which can achieve real time online monitoring of organizations＇ energy consumption via data collection, and also proposes the design idea of energy data acquisition based on national standards.
基金supported by The Construction Program of Shanghai Engineering Research Center(Grant number 17DZ2251800).
文摘Bamboo Winding Composite Pipes(BWCPs)have many advantages such as good mechanical properties,low cost,low carbon,environmental friendliness,thermal insulation and easy installation et al.At present,BWCP is widely used in urban water supply,drainage,communication cable protection,farmland irrigation etc.It is obvious that the Bamboo Winding Composite materials have good application prospects in the field of utility tunnels.However,considering that the size of utility tunnels is much larger than that of normal pipelines,the wide application of Bamboo Composite Utility Tunnels(BCUTs)is still a challenge.In this paper,the force performance of BCUT in Datong Shanxi Province,was studied using two methods:real time monitoring and numerical simulation.Wireless sensor networks were used for monitoring,and the real time monitoring data of horizontal convergence and strains under the condition of backfill were obtained.The monitoring data indicated that the horizontal convergence can meet the requirement of relevant technical standards.A three-dimensional numerical model of the utility tunnel was established using FEM software.The influence of soil parameters on the deformation and strain of the BCUT was studied using the numerical method.The simulation results were compared with real-time monitoring data.The soil parameters K_(0) and k_(sh) have significant effects on the deformation,stress and strain of the utility tunnel.This kind of utility tunnel is recommended to use in areas with relatively good geological conditions.
基金the financial support by the International Science&Technology Collaboration Project from Ministry of Science and Technology of the People’s Republic of China(2014DFG72150)Program for New Century Excellent Talents in University from MOE of the People's Republic of China(NCET-12-0473).
文摘Rapidly acquiring and real-time transmitting crop water requirement information constitute the basis for achieving intelligent diagnosis and precision irrigation.In order to collect and transmit crop water requirement information at real time,a new microcontroller-based real-time remote monitoring system was designed,including system hardware design,software and anti-jamming design.The system achieved the functions including clock reading,information configuration,LCD display,keyboard control,data sending and receiving,multi-channel information acquisition,conversion and storage.Laboratory and field tests showed that the system can achieve data acquisition and real-time display of the crop water requirement information.Unlike the current weather station,the system collects crop water information,meteorological factors and soil parameters at the same time.It has a high level of stability and acquisition accuracy,and can meet the requirements for real-time remote monitoring of the crop water requirement information for irrigation decision-making.
基金the financial support of joint project(#5016)of Georgian National Scientific Foundation(GNSF)and ScienceTechnology Center of Ukraine(STCU)and Open Partial Agreement on the Major Disasters at the Council of Europe(EUR-OPA).
文摘Large dams are complex structures with nonlinear dynamic behavior.Engineers often are forced to assess dam safety based on the available incomplete data,which is extremely difficult.This important problem can be solved with the modern theory of complex systems.It is possible to derive characteristics of the whole unknown dynamics of a structure using few data sets of certain carefully selected representative parameter(s).By means of high quality continuous records of some geotechnical characteristic(s)of a dam and modern methods of time series linear/nonlinear analysis the main dynamical features of the entire,unknown process(here—dam deformation)can be analyzed.We created the cost-effective Monitoring Telemetric System for Dam Diagnostics(DAMWATCH),which consists of sensors(tiltmeters),terminal and central controllers connected by the GSM/GPRS Modem to the diagnostic center.The tilt data recorded for varying reservoir level are compared with static design model of dam deformations computed by a finite element method(FEM)for the dam-reservoir-foundation system.Besides,recently developed linear/nonlinear data analysis and prediction schemes may help to quantify fine dynamical features of the dam behavior.The software package DAMTOOL has been developed for this purpose.The differences between measured and theoretically predicted response parameters of the dam may signal abnormal behavior of the object.The data obtained already by testing of the DAMWATCH/DAMTOOL system during operation of the high Enguri arc dam and reservoir(Georgia)show interesting long-term and short-term patterns of tilts in the dam body,which can be used for dam diagnostics.The proposed real-time telemetric monitoring(DAMWATCH)complex and linear/nonlinear dynamical analysis system(DAMTOOL)are unique.
文摘We propose a technique for chromatic dispersion monitoring based on optical time domain level monitoring. Experimental and simulation results show that the technique is effective for the monitoring of dispersion in 42.7-Gbps CS-RZ signals for dynamic dispersion compensation.
文摘Compared to small molecule process analytical technology （PAT） applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell culture process is essential to establish an appropriate process control strategy for biotechnology product PAT applications. Inline spectroscopic techniques for real time monitoring of bioreactor cell culture process have the distinct potential to develop PAT approaches in manufac- turing biotechnology drug products. However, the use of inline Fourier transform infrared （FTIR） spectroscopic techniques for bioreactor cell culture process monitoring has not been reported. In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell culture fluid biomolecular dynamic model. The technical feasibility of using FTIR Spectroscopy for real time tracking and monitoring four key cell culture metabolites （including glucose, glutamine, lactate, and ammonia） and protein yield at increasing levels of complexity （simple binary system, fully formulated media, actual bioreactor cell culture process） was evaluated via a stepwise approach. The FTIR fingerprints of the key metabolites were identified. The multivariate partial least squares （PLS） calibration models were established to correlate the process FTIR spectra with the concentrations of key metabolites and protein yield of in-process samples, either individually for each metabolite and protein or globally for all four metabolites simultaneously. Applying the 2＇ld derivative pre-processing algorithm to the FTIR spectra helps to reduce the number of PLS latent variables needed significantly and thus simplify the interpretation of the PLS models. The validated PLS models show promise in predicting the concentration profiles of glucose, glutamine, lactate, and ammonia and protein yield over the course of the bioreactor cell culture process. Therefore, this work demonstrated the technical feasibility of real time monitoring of the bioreactor cell culture process via FTIR spectroscopy. Its implications for enabling cell culture PAT were discussed.
基金supported by the National Key Research and Development Program of China(2017YFA050400 and2017YFC0906900)the National Natural Science Foundation of China(31722033,91649123,31671484,31225008,and 31470833)+4 种基金the Shanghai Science and Technology Commission(14XD1401400,16430723100,and 15YF1402600)Young Elite Scientists Sponsorship Program by China Association for Science and Technology(to YZ)Shanghai Young Top-notch Talent(to YZ)the State Key Laboratory of Bioreactor Engineering(to YY)Fundamental Research Funds for the Central Universities(to YY and YZ)
文摘The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte- neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neu- ronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here,we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.
基金supported by Governmental Public Industry Research Special Funds for Projects(201301016)National Key Technology R&D Program of the Ministry of Science and Technology(2012BAH29B04-02)+1 种基金Chinese Universities Scientific Fund,Northwest A&F University(QN2011132)the Introduction of Intelligence from Abroad Project for Innovation in Academic Schools,China(B12007).
文摘Normalized Difference Vegetation Index(NDVI)has been found to have good correlations with many physical properties of soybean surfaces.Due to the factors of air temperature,humidity,solar radiation,soil moisture,etc.,NDVI of soybean varies dynamically in a day.The establishment of the soybean NDVI prediction model at different times in a day can effectively modify this variation.The soybean NDVI values are continuously monitored in hours during soybean seeding,flowering&podding and maturating stages by way of Green Seeker.Results show that the trend of NDVI change every day in the three stages is taken on as a reverse parabola.The NDVI value reaches to the maximum at 8 am or 9 am and decreases to its minimum at 2 pm before a moderate rise.A model for intraday and long-term NDVI change for soybean is built.The test of the model with independent data indicates that the precision meets the demands,with the root mean square error(RMSE)of each day being 3.95,5.45 and 2.86 for the seeding stage,the bean podding stage and the maturation period,respectively.The prediction RMSEs of the soybean NDVI model for soybeans of the three stages for the fifth day are 5.75,2.65 and 5.51,respectively and the prediction RMSEs for the sixth day are 9.74,2.82 and 14.04,respectively according to the data from the first four days.
