The Ninety East Ridge in the Indian Ocean has complex and unique characteristics.The concentrations and distribution characteristics of 10 trace metals(V,Cr,Mn,Fe,Co,Ni,Cu,Cd,Pb,and U)in seawater from the Ninety East ...The Ninety East Ridge in the Indian Ocean has complex and unique characteristics.The concentrations and distribution characteristics of 10 trace metals(V,Cr,Mn,Fe,Co,Ni,Cu,Cd,Pb,and U)in seawater from the Ninety East Ridge in the Indian Ocean were investigated.Results show that the average concentrations of different trace metals in all the collected seawater samples were 1.134μg/L for V,0.158μg/L for Cr,0.489μg/L for Mn,0.427μg/L for Fe,0.011μg/L for Co,0.395μg/L for Ni,0.403μg/L for Cu,0.097μg/L for Cd,0.139μg/L for Pb,and 3.470μg/L for U.Differences in the horizontal and vertical distributions of all measured trace metals were revealed,and the occurrence of high concentrations was nonuniform.In addition,the significant differences in the concentration distribution of different trace metals in seawater on both sides of the Ninety East Ridge present regional segmentation in the area for various trace metals in deep sea water.This study provided basic data for future investigations on the environmental and ecological impact of trace metals in the Indian Ocean and the potential water mass transport mechanism.展开更多
This study aimed to investigate the pollution characteristics, source apportionment, and health risks associated with trace metal(loid)s(TMs) in the major agricultural producing areas in Chongqing, China. We analyzed ...This study aimed to investigate the pollution characteristics, source apportionment, and health risks associated with trace metal(loid)s(TMs) in the major agricultural producing areas in Chongqing, China. We analyzed the source apportionment and assessed the health risk of TMs in agricultural soils by using positive matrix factorization(PMF) model and health risk assessment(HRA) model based on Monte Carlo simulation. Meanwhile, we combined PMF and HRA models to explore the health risks of TMs in agricultural soils by different pollution sources to determine the priority control factors. Results showed that the average contents of cadmium(Cd), arsenic (As), lead(Pb), chromium(Cr), copper(Cu), nickel(Ni), and zinc(Zn) in the soil were found to be 0.26, 5.93, 27.14, 61.32, 23.81, 32.45, and 78.65 mg/kg, respectively. Spatial analysis and source apportionment analysis revealed that urban and industrial sources, agricultural sources, and natural sources accounted for 33.0%, 27.7%, and 39.3% of TM accumulation in the soil, respectively. In the HRA model based on Monte Carlo simulation, noncarcinogenic risks were deemed negligible(hazard index <1), the carcinogenic risks were at acceptable level(10^(-6)<total carcinogenic risk ≤ 10^(-4)), with higher risks observed for children compared to adults. The relationship between TMs, their sources, and health risks indicated that urban and industrial sources were primarily associated with As, contributing to 75.1% of carcinogenic risks and 55.7% of non-carcinogenic risks, making them the primary control factors. Meanwhile, agricultural sources were primarily linked to Cd and Pb, contributing to 13.1% of carcinogenic risks and 21.8% of non-carcinogenic risks, designating them as secondary control factors.展开更多
The economy of West African countries is mainly based on agriculture. However, the trace metal(loid)s contamination status in rivers is relatively unknown in the region. In this work, 45 surface sediments collected fr...The economy of West African countries is mainly based on agriculture. However, the trace metal(loid)s contamination status in rivers is relatively unknown in the region. In this work, 45 surface sediments collected from the Bandama, Comoé, and Bia Rivers in south and south eastern Côte d’Ivoire (West Africa), were analyzed for total metal concentrations and chemical speciation. The results showed that the river sediments were considerably contaminated by Cd and moderately contaminated by As, Cu, Pb, and Zn. Significant spatial variations were observed among the stations but not between the rivers. Metals Cd and Cu were likely to cause more ecological risks. The speciation analysis unravelled that the metal(loid)s partitioned mainly in the residual fraction, with the potential mobile fraction varying from 14% to 28%. The study calls for establishment of strict policies relative to the application of fertilizers and agrochemicals and mining activities to protect the environment and human health risks.展开更多
The present study was carried out on small-scale gold mining sites in the Yaouré region of Côte d’Ivoire. This region is geologically representative of the Birimian formations (2.1 Ga) of West Africa. The a...The present study was carried out on small-scale gold mining sites in the Yaouré region of Côte d’Ivoire. This region is geologically representative of the Birimian formations (2.1 Ga) of West Africa. The aim is to determine the potentially toxic trace metals (TMEs) generated by these sites, with a view to preventing possible contamination and/or metal pollution of the waters that provide fish products for local populations. To this end, a sampling campaign was carried out, resulting in the collection of 20 mining waste samples analyzed by X-ray fluorescence spectrometry (XRF) and 10 by X-ray diffractometer (XRD). The XRF analysis detected 06 predominant TMEs: arsenic, chromium, copper, nickel, zinc and vanadium. Statistical analysis was carried out to determine the distributions and correlations between these ETMs. To assess contamination and/or pollution levels, the following indices were calculated on the basis of reference concentrations of upper continental crust MTEs: Enrichment Factor, Geo-accumulation Index, Concentration Factor, Degree of contamination and those related to ecological risks. The results of statistical analyses and indices have shown that arsenic and chromium are the most predominant and can be, depending on the chemical form, potentially more toxic. The results of the DRX analysis show the occurrence of several minerals carrying these two MTEs, especially that of a rare mineral, Stenhuggarite, an arsenic oxide linked to hydrothermal veins. The majority of gold mining operations in West Africa are located in the birimian zone, hence the need for environmental monitoring by the relevant authorities, to prevent potential ecological risks to water and possibly health risks via the food chain.展开更多
Arsenic,barium,bismuth,cadmium,cesium,chromium,cobalt,copper,iron,lead,manganese,molybdenum,nickel,strontium,thallium,tungsten,uranium,vanadium,and zinc concentrations were investigated in forty-two groundwater sample...Arsenic,barium,bismuth,cadmium,cesium,chromium,cobalt,copper,iron,lead,manganese,molybdenum,nickel,strontium,thallium,tungsten,uranium,vanadium,and zinc concentrations were investigated in forty-two groundwater samples,twenty-four surface water and six surface sediment samples in Kinsevere industrial zone and its surroundings in February and march 2017,January,February and March 2018 to evaluate the potential human health risk.Chemical analyses were carried out by using ICP-SF-MS(Inductively Coupled Plasma-Sector Field Mass Spectrometry,Thermo Scientific Element II).The trace metals were detected at various concentrations in all the analyzed samples.Pb,Mn and Fe concentrations exceeded the European Union acceptable maximum limits for water intended for human consumption in 4.76%,28.57%and 61.90%of the groundwater samples,respectively and in 0%,50%and 100%of the surface water samples,respectively.As,Cr,Cu and Ni concentrations exceeded the recommended lower sediment quality guideline values in 33.33%,50%,83.33%and 83.33%of the surface sediment samples,respectively.All those elevated trace metal concentrations in the groundwater,surface water and sediments represent a risk for the health of local population as well as for aquatic organisms.展开更多
Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxy...Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxygen demand, salinity of the sea surface microlayer and subsurface water. Apparent enrichment mechanism and diurnal variation have been revealed for dissolved trace metals in the microlayer in Daya Bay. The more dissolved organic matter was enriched in the sea surface microlayer, the more dissolved trace metals were enriched in the layer. The organic matter played an important role in the enrichment process. The diurnal variations of dissolved trace metals showed that their concentration was apparently inversely related to the tide activity that the concentration was low during rising tide, but high during falling tide. The behavior of dissolved trace metals expressed by the diurnal variation was clearly opposite to that of salinity.展开更多
To examine the degree of contamination in the Dongting Lake （洞庭湖） area, surface sediments samples were collected and investigated in this study. This lake lies in the south of the middle Yangtze River, and it is ...To examine the degree of contamination in the Dongting Lake （洞庭湖） area, surface sediments samples were collected and investigated in this study. This lake lies in the south of the middle Yangtze River, and it is the second largest fresh-water lake in China. These samples were analyzed for the concentrations of the following 6 elements （CA, Cr, Cn, Ni, Ph and Zn） collected from 46 locations. The index of geo-accumulation was used to assess the degree of contamination of the total trace elements and the assessments showed that the contamination of Cd reached strong to very strong pollution. The speciation of metal in sediments was analyzed using the modified BCR （Community Bureau of Reference） sequential extraction technology and the water column-contamination risk was evaluted by the calculated contamination factors. The results of the speciation of metal indicated that more easily mobilized forms （exchangeable, water soluble, and acid soluble） were predominant for CA, especially in the samples from East Dongting Lake. In contrast, the largest amount of lead was associated with the iron and manganese oxide fractions, and Cn, Zn, Cr, and Ni analyzed were mainly distributed in residual phase at an average percentage higher than 60 % of the total metals. The potential risk to the lake＇s water contamination was the highest in East Dongting Lake based on the calculated contamination factors. The assessment results using geo-accamnlation index were compared with the information obtained by trace metal speciation and both results were consistent.展开更多
Physicochemical properties, total and DTPA （diethylenetriaminepentaacetic acid）-extractable Cu, Zn, Pb and Cd contents, microbial biomass carbon （C） content and the organic C mineralization rate of the soils in a ...Physicochemical properties, total and DTPA （diethylenetriaminepentaacetic acid）-extractable Cu, Zn, Pb and Cd contents, microbial biomass carbon （C） content and the organic C mineralization rate of the soils in a long-term trace metal-contaminated paddy region of Guangdong, China were determined to assess the sensitivity of microbial indices to moderately metal-contaminated paddy soils. The mean contents of total Cu, Zn, Pb and Cd were 251, 250, 171, and 2.4 mg kg^-1 respectively. DTPA-extractable metals were correlated positively and significantly with total metals, CEC, and organic C （except for DTPA-extractable Cd）, while they were negatively and highly significantly correlated with pH, totall Fe and Mn. Metal stress resulted in relatively low ratios of microbial biomass C to organic C and in remarkable inhibition of the microbial metabolic quotient and C mineralization rate, which eventually led to increases in soil organic C and C/N. Moreover, microbial respiratory activity showed a stronger correlation to DTPA-extractable metals than to total metal content. Likewise, in the acid paddy soils some “linked” microbial activity indices, such as metabolic quotient and ratios of basal respiration to organic C, especially during initial incubation, were found to be more sensitive indicators of soil trace metal contamination than microbial biomass C or basal respiration alone.展开更多
Four short cores were obtained from the coastal wetland of the Quanzhou Bay,Fujian Province,China,and sediment samples were analyzed with a Mastersizer 2000 for grain size analysis and an Inductively Coupled Plasma At...Four short cores were obtained from the coastal wetland of the Quanzhou Bay,Fujian Province,China,and sediment samples were analyzed with a Mastersizer 2000 for grain size analysis and an Inductively Coupled Plasma Atomic Emission Spectrometer(ICP-AES) for trace metal analysis. The results of grain size analysis show that the wetland sediments are mainly composed of silt and clay,and the distribution of median grain size is affected by human activities obviously. The results of trace metal analysis show that the wetland sediments mainly originate from terrestrial materials in the Luoyangjiang River estuary and from contaminants discharged from adjacent rivers in Shuitou area. The heavy metal contamination in Luoyangjiang estuary decreased from 1984 to 2004 due to industry transformation,but has increased since 2004 because of the discharge of contaminants to the Jinjiang River and much living sewerage to the bay,and a large number of trace metals are trapped within Spartina alterniflora marsh.展开更多
Selected trace metals （Pb, Cd, Cu, Hg） and arsenic in seawater and surface sediments of Laizhou Bay were determined, to evaluate their spatial distribution, pollution risk and potential ecological risk. Concentratio...Selected trace metals （Pb, Cd, Cu, Hg） and arsenic in seawater and surface sediments of Laizhou Bay were determined, to evaluate their spatial distribution, pollution risk and potential ecological risk. Concentrations of the elements were 0.56-2.07, 0.14-0.38, 12.70-18.40, 0.014-0.094, and 1.13- 2.37 μg/L in the seawater and 8.94-32.2, 0.18-0.67, 4.51-30.5, 0.006-0.058, and 5.75-15.3 mg/kg in sediments for Pb, Cd, Cu, Hg and As, respectively. High concentrations of the trace metals and arsenic in seawater and surface sediments were generally observed near the fiver estuary. The pollution risk result of the elements showed that Cu was the prominent trace metal pollutant in seawater, followed by Hg, Pb, Cd and As. The metal complex pollution index in seawater was at a medium level. The most important trace metal pollutant in sediments was Cd, followed by As, Cu, Pb, and Hg. Our pollution assessment suggests that trace metal pollution in Laizhou Bay sediments was at a low level. The potential ecological risk was also low in surface sediment.展开更多
A method based on the combination of coprecipitation with inductively coupledplasma atomic emission spectrometry (ICP-AES) was developed for the determination of impurities inhigh-purity sodium tungstate. Six elements...A method based on the combination of coprecipitation with inductively coupledplasma atomic emission spectrometry (ICP-AES) was developed for the determination of impurities inhigh-purity sodium tungstate. Six elements (Co, Cu, Fe, Mn, Ni, and Pb) were coprecipitated bylanthanum hydroxide so as to be concentrated and separated from the tungsten matrix. Effects of somefactors on the recoveries of the analytes and on the residual amount of sodium tungstate wereinvestigated, and the optimum conditions for the coprecipitation were proposed. Matrix-matchingcalibration curve method was used for the analysis. It is shown that the elements mentioned abovecan be quantitatively recovered. The detection limits for Co, Cu, Fe, Mn, Ni, and Pb are 0.07, 0.4,0.2, 0.1, 0.6, and 1.3 μg·g^(-1), respectively. The recoveries vary from 92.5% to 108%, and therelative standard deviations (RSDs) are in the range of 3.1%-5.5%.展开更多
This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven tr...This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-MS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEM–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent DOM component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and Mg were significantly higher than Ca,Fe,Mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.展开更多
The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The m...The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The mean enrichment factors ([Xi]microlayer/[Xi ]15cm in depth) mostly ranged between 1 . 0 and 2. 0. The dissolved forms were the major forms of the components measured, the enrichment of dissolved organic matter and suspended particles could lead to the changes in the total amount and speciation of nutrients and trace metals. No correlation was observed between sample concentrations, speciation, enrichment factors and sample locations. However, some evidence shows that these parameters are correlated with sea state, indicating the complexity and dynamic nature of the sea surface microlayer.展开更多
AIM: To assess whether trace metal concentrations (which influence metabolism as both essential and non-essential elements) are increased or decreased in cancerous tissues and to understand the precise role of these m...AIM: To assess whether trace metal concentrations (which influence metabolism as both essential and non-essential elements) are increased or decreased in cancerous tissues and to understand the precise role of these metals in carcinogenesis. METHODS: Concentrations of trace metals including Cd, Ni, Cu, Zn, Fe, Mg and Ca in both cancerous and non- cancerous stomach tissue samples were determined by atomic absorption spectrometry (AAS). Tissue samples were digested using microwave energy. Slotted tube atom trap was used to improve the sensitivity of copper and cadmium in flame AAS determinations. RESULTS: From the obtained data in this study, the concentrations of nickel, copper and iron in the cancerous human stomach were found to be significantly higher than those in the non-cancerous tissues, by using t-test for the paired samples. Furthermore, the average calcium concentrations in the cancerous stomach tissue samples were found to be significantly lower than those in the non-cancerous stomach tissue samples by using t-test. Exceedingly high Zn concentrations (207-826 mg/ kg) were found in two paired stomach tissue samples from both cancerous and non-cancerous parts. CONCLUSION: In contrast to the literature data for Cu and Fe, the concentrations of copper, iron and nickel in cancerous tissue samples are higher than those in the non-cancerous samples. Furthermore, the Ca levels are lower in cancerous tissue samples than in non-cancerous tissue samples.展开更多
Historical records of metal inputs were studied by using a sediment core collected from a sand-rich mudflat in the Qinjiang River estuary, China. 210Pb chronology was used to reconstruct the fluxes of Hg, Cu, Pb, Zn, ...Historical records of metal inputs were studied by using a sediment core collected from a sand-rich mudflat in the Qinjiang River estuary, China. 210Pb chronology was used to reconstruct the fluxes of Hg, Cu, Pb, Zn, Cd, Cr and As to the core site during the last 86 years. Based on the constant initial concentration model, the sedimentation rates are 1.18 cm year-1 in the top 30 cm sandy layer and 0.92 cm year-1 in the muddy bottom layer. To compensate for grain-size and mineralogy effects on metal concentra-tions, aluminum was used as the normalizing element. The enrichment factors (EF) indicate that the natural inputs had prevailed up to the early 1980s. After this period, the intensity of human activities has resulted in continual increasing trend of metals towards the surface. Recent sediment samples from the Qinjiang River estuary are found moderately enriched by Cd (EF>1.5) and slightly enriched by other metals (EF<1.5). Considering that the drainage area of the Qinjiang River is mostly agricultural land, the increased Cd may be due to the usage of fertilizers and pesticides in agricultural activities and the combustion of fossil fuels.展开更多
The long-term seepage of hydrocarbons, either as macroseepage or microseepage, can set up near-surface oxidation reduction zones that favor the development of a diverse array of chemical and mineralogical changes. The...The long-term seepage of hydrocarbons, either as macroseepage or microseepage, can set up near-surface oxidation reduction zones that favor the development of a diverse array of chemical and mineralogical changes. The bacterial oxidation of light hydrocarbons can directly or indirectly bring about significant changes in the values of pH and Eh of the surrounding environment, thereby also changing the stability fields of the different mineral species present in that environment. The paper reports the role of hydrocarbon microseepage in surface alterations of trace metal concentrations. In this study trace metal alterations were mapped that appear to be associated with hydrocarbon microseepages in the oil/ gas fields. A total of 50 soil samples were collected near oil and gas fields of the Tatipaka and Pasarlapudi areas of the Krishna Godavari Basin, Andhra Pradesh. The soil samples were collected from a depth of 2-2.5 m. The paper reports the chemical alterations associated with trace metals in soils that are related to hydrocarbon microseepages above some of the major oil and gas fields of this petroliferous region. Trace metals, such as scandium （Sc）, vanadium （V）, chromium （Cr）, cobalt （Co）, nickel （Ni）, copper （Cu）, zinc （Zn）, barium （Ba） and strontium （Sr）, in soil samples were analyzed using inductively coupled plasma- mass spectrometry （ICP-MS）. The concentrations of Sc （8 to 40 mg/kg）, V （197 to 489 mg/kg）, Cr （106 to 287 mg/kg）, Co （31 to 52 mg/kg）, Ni （65 to 110 mg/kg）, Cu （88 to 131 mg/kg）, Zn （88 to 471 mg/kg）, Ba （263 to 3,091 mg/kg） and Sr （119 to 218 mg/kg） were obtained. It was observed that the concentrations of trace elements were tremendously increased when they were compared with their normal concentrations in soils. The analysis of adsorbed soil gas showed the presence of high concentrations of YC2＋ （C2H6, C3H8 and n-C4H10） ranging from 7 to 222 μg/kg respectively. Integrated studies of trace elements over adsorbed light gaseous hydrocarbons （2C2＋） anomalies showed good correlation with the existing oil and gas wells. The carbon isotopic composition of δ13C1 of the samples ranges between -36.6%o to -22.7‰ （Pee Dee Belemnite） values indicate thermogenic origin, which presents convincing evidence that the adsorbed soil gases collected from these sediments are of catagenetic origin. The increase in the concentrations of trace metals near oil/gas producing areas, suggests a soil chemical change to a reducing environment, presumably due to the influence of hydrocarbon microseepage, which could be applied with other geoscientific data to identify areas of future hydrocarbon exploration in frontier areas.展开更多
Objective To determine the metal contents of lichen species from East Black Sea region of Turkey for investigation of trace metal pollution sourced traffic. Methods The levels of copper, cadmium, lead, zinc, manganese...Objective To determine the metal contents of lichen species from East Black Sea region of Turkey for investigation of trace metal pollution sourced traffic. Methods The levels of copper, cadmium, lead, zinc, manganese, iron, chromium, nickel, cobalt, palladium in lichen samples collected from East Black Sea region of Turkey were determined by flame and graphite furnace atomic absorption spectrometry after microwave digestion method. The accuracy of the method was corrected by standard reference material （NIST SRM IAEA-336 Lichen）. Results The contents of invest!gated trace metals in lichen samples were 7.19-22.4 μg/g for copper, 0.10-0.64 μg/g for cadmium, 4.03-44.6 μg/g for lead, 14.5-41.8 μg/g for zinc, 25.8-208 μg/g for manganese, 331-436 μg/g for iron, 1.20-3.01 μg/g for chromium, 1.48-3.90 μg/g for nickel, 0.20-3.55 μg/g for cobalt, 0.11-0.64 μg/g for palladium. The results were compared with the literature values. Conclusion Some lichen species such as Xanthoparmelia conspersa, Xanthoria calcicola, Peltigera membranacea, and Physcia adscendens are accumulated trace metals at a high ratio.展开更多
AIM: To investigate the oxidative-stress-related changes in rats with portal hypertension with particular emphasis on nitric oxide (NO) and trace metals. METHODS: Cirrhosis was induced by partial portal vein ligation ...AIM: To investigate the oxidative-stress-related changes in rats with portal hypertension with particular emphasis on nitric oxide (NO) and trace metals. METHODS: Cirrhosis was induced by partial portal vein ligation (PVL) in Wistar rats. The lipid peroxidation marker (malondialdehyde, MDA), antioxidant defense enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and agents known to have antioxidant features including nitric oxide (NO), zinc (Zn), copper (Cu) were determined both in serum and in liver tissue at 4 wk after surgery in PVL and sham-operated rats. Portal pressure of all experimental animals was measured. MDA was detected by thiobarbituric acid reactivity assay.SOD activity was determined by inhibition of nitroblue tetrazolium reduction with xanthine/xanthine oxidase used asa superoxide generator. CAT activity was determined by the breakdown of hydrogen peroxide. GSH concentrations were measured by using metaphosphoric acid for protein precipitation and 5'-5'-dithio-bis-2-nitrobenzoic acid for color development. NO was detected by the Griess method after reduction of nitrate to nitrite with nitrate reductase, and the concentrations of Zn and Cu were measured by a Shimadzu 680 AA atomic absorption spectrometer. Histopathological confirmation was done under light microscope. Statistical analyses were done by Student's t-test, and significance of the difference was tested by the unpaired Mann-Whitney test. P＜0.05 was considered statistically significant.RESULTS: Histopathological studies confirmed PVL induced cirrhotic changes. There was a statistically significant difference in portal pressure between PVL and control groups (P＜0.001). The results showed significant increases in the levels of MDA and NO in both tissue and serum (P＜0.05 and P＜0.001, respectively in tissue; P＜0.001 for each in serum), and Zn only in tissue (P＜0.001)in rats with PVL compared with sham-operated rats. Besides, PVL rats exhibited reduced plasma and tissue GSH, CAT, SOD (P＜0.001 for each). Serum and tissue Cu concentration did not change.CONCLUSION: Our findings suggest that PVL in rats induces important biochemical and molecular changes related to oxidative stress in the liver.展开更多
This study is aimed at determining the level of environmental degradation as well as the concentration of trace elements in soil and stream sediments in order to evaluate the environmental impact of the mining operati...This study is aimed at determining the level of environmental degradation as well as the concentration of trace elements in soil and stream sediments in order to evaluate the environmental impact of the mining operation.Twenty-five(25) soils and ten(10) stream sediment samples were collected from the study area. The physicochemical parameters were determined using appropriate instrumentation with the aid of a digital p H meter(Milwaukee meter) to measure the p H and electrical conductivity, total dissolved solids, moisture content and loss on ignition of the soil and stream sediment samples. The p H of the soil sample ranged from(6.10 to 7.19); Electrical conductivity ranged from(21.3 to 279.4 μS/cm), moisture content varied from(0.60% to 7.20%), and the LOI ranged from(2.03% to 18.62%). The results of the analysis showed that the concentrations of the trace elements in the soils and stream sediment samples were slightly higher than the background values. Plots of the trace elements in stream sediment samples show moderate, consistent decrease downstream except at points where there was mine water discharge into the main river. The pollution levels of heavy metals were examined in stream sediment and soil samples using different assessable indices, such as the enrichment factor, which showed significant-moderate enrichment for Cr, Th, Nb, Zn, Pb, Y and Zr and the geoaccumulation index, which showed practically moderate contamination with Cr, Ni and Sr based on regional background reference values. Geo-accumulation index and contamination index for soils and stream sediment revealed uncontaminated to moderate contamination. Likewise,elements with moderate contamination were Cr, Ni and Sr.The Pearson correlation showed that there were significant positive associations among selected metals in soil and stream sediment samples.展开更多
Coral bleaching has generally been recognized as the main reason for tropical coral reef degradation, but there are few long-term records of coral bleaching events. In this study, trace metals including chromium(Cr), ...Coral bleaching has generally been recognized as the main reason for tropical coral reef degradation, but there are few long-term records of coral bleaching events. In this study, trace metals including chromium(Cr), copper(Cu), molybdenum(Mo), manganese(Mn), lead(Pb), tin(Sn), titanium(Ti), vanadium(V), and yttrium(Y), were analyzed in two Porites corals collected from Meiji Reef in the tropical South China Sea(SCS) to assess differences in trace metal concentrations in bleached compared with unbleached coral growth bands. Ti, V, Cr, and Mo generally showed irregular fluctuations in both corals. Bleached layers contained high concentrations of Mn, Cu, Sn, and Pb. Unbleached layers showed moderately high concentrations of Mn and Cu only. The different distribution of trace metals in Porites may be attributable to different selectivity on the basis of vital utility or toxicity. Ti, V, Cr, and Mo are discriminated against by both coral polyps and zooxanthellae, but Mn, Cu, Sn, and Pb are accumulated by zooxanthellae and only Mn and Cu are accumulated by polyps as essential elements. The marked increase in Cu, Mn, Pb, and Sn are associated with bleaching processes, including mucus secretion, tissue retraction, and zooxanthellae expulsion and occlusion. Variation in these trace elements within the coral skeleton can be used as potential tracers of short-lived bleaching events.展开更多
基金Supported by the Natural Science Foundation of Shandong Province(No.ZR2021MD079)the APEC Cooperation Fund(No.WJ1323001)the Asian Cooperation Fund(No.WJ1223001)。
文摘The Ninety East Ridge in the Indian Ocean has complex and unique characteristics.The concentrations and distribution characteristics of 10 trace metals(V,Cr,Mn,Fe,Co,Ni,Cu,Cd,Pb,and U)in seawater from the Ninety East Ridge in the Indian Ocean were investigated.Results show that the average concentrations of different trace metals in all the collected seawater samples were 1.134μg/L for V,0.158μg/L for Cr,0.489μg/L for Mn,0.427μg/L for Fe,0.011μg/L for Co,0.395μg/L for Ni,0.403μg/L for Cu,0.097μg/L for Cd,0.139μg/L for Pb,and 3.470μg/L for U.Differences in the horizontal and vertical distributions of all measured trace metals were revealed,and the occurrence of high concentrations was nonuniform.In addition,the significant differences in the concentration distribution of different trace metals in seawater on both sides of the Ninety East Ridge present regional segmentation in the area for various trace metals in deep sea water.This study provided basic data for future investigations on the environmental and ecological impact of trace metals in the Indian Ocean and the potential water mass transport mechanism.
基金supported by Project of Chongqing Science and Technology Bureau (cstc2022jxjl0005)。
文摘This study aimed to investigate the pollution characteristics, source apportionment, and health risks associated with trace metal(loid)s(TMs) in the major agricultural producing areas in Chongqing, China. We analyzed the source apportionment and assessed the health risk of TMs in agricultural soils by using positive matrix factorization(PMF) model and health risk assessment(HRA) model based on Monte Carlo simulation. Meanwhile, we combined PMF and HRA models to explore the health risks of TMs in agricultural soils by different pollution sources to determine the priority control factors. Results showed that the average contents of cadmium(Cd), arsenic (As), lead(Pb), chromium(Cr), copper(Cu), nickel(Ni), and zinc(Zn) in the soil were found to be 0.26, 5.93, 27.14, 61.32, 23.81, 32.45, and 78.65 mg/kg, respectively. Spatial analysis and source apportionment analysis revealed that urban and industrial sources, agricultural sources, and natural sources accounted for 33.0%, 27.7%, and 39.3% of TM accumulation in the soil, respectively. In the HRA model based on Monte Carlo simulation, noncarcinogenic risks were deemed negligible(hazard index <1), the carcinogenic risks were at acceptable level(10^(-6)<total carcinogenic risk ≤ 10^(-4)), with higher risks observed for children compared to adults. The relationship between TMs, their sources, and health risks indicated that urban and industrial sources were primarily associated with As, contributing to 75.1% of carcinogenic risks and 55.7% of non-carcinogenic risks, making them the primary control factors. Meanwhile, agricultural sources were primarily linked to Cd and Pb, contributing to 13.1% of carcinogenic risks and 21.8% of non-carcinogenic risks, designating them as secondary control factors.
文摘The economy of West African countries is mainly based on agriculture. However, the trace metal(loid)s contamination status in rivers is relatively unknown in the region. In this work, 45 surface sediments collected from the Bandama, Comoé, and Bia Rivers in south and south eastern Côte d’Ivoire (West Africa), were analyzed for total metal concentrations and chemical speciation. The results showed that the river sediments were considerably contaminated by Cd and moderately contaminated by As, Cu, Pb, and Zn. Significant spatial variations were observed among the stations but not between the rivers. Metals Cd and Cu were likely to cause more ecological risks. The speciation analysis unravelled that the metal(loid)s partitioned mainly in the residual fraction, with the potential mobile fraction varying from 14% to 28%. The study calls for establishment of strict policies relative to the application of fertilizers and agrochemicals and mining activities to protect the environment and human health risks.
文摘The present study was carried out on small-scale gold mining sites in the Yaouré region of Côte d’Ivoire. This region is geologically representative of the Birimian formations (2.1 Ga) of West Africa. The aim is to determine the potentially toxic trace metals (TMEs) generated by these sites, with a view to preventing possible contamination and/or metal pollution of the waters that provide fish products for local populations. To this end, a sampling campaign was carried out, resulting in the collection of 20 mining waste samples analyzed by X-ray fluorescence spectrometry (XRF) and 10 by X-ray diffractometer (XRD). The XRF analysis detected 06 predominant TMEs: arsenic, chromium, copper, nickel, zinc and vanadium. Statistical analysis was carried out to determine the distributions and correlations between these ETMs. To assess contamination and/or pollution levels, the following indices were calculated on the basis of reference concentrations of upper continental crust MTEs: Enrichment Factor, Geo-accumulation Index, Concentration Factor, Degree of contamination and those related to ecological risks. The results of statistical analyses and indices have shown that arsenic and chromium are the most predominant and can be, depending on the chemical form, potentially more toxic. The results of the DRX analysis show the occurrence of several minerals carrying these two MTEs, especially that of a rare mineral, Stenhuggarite, an arsenic oxide linked to hydrothermal veins. The majority of gold mining operations in West Africa are located in the birimian zone, hence the need for environmental monitoring by the relevant authorities, to prevent potential ecological risks to water and possibly health risks via the food chain.
基金This study was carried out in the framework of the research project entitled“Assessing and Mapping the Environmental and Health Impacts of Abandoned and Ongoing Mining Activities in Lubumbashi and Its Neighborhood,Democratic Republic of Congo”with UNESCO-Sida Project funds(Contract No.4500309530).
文摘Arsenic,barium,bismuth,cadmium,cesium,chromium,cobalt,copper,iron,lead,manganese,molybdenum,nickel,strontium,thallium,tungsten,uranium,vanadium,and zinc concentrations were investigated in forty-two groundwater samples,twenty-four surface water and six surface sediment samples in Kinsevere industrial zone and its surroundings in February and march 2017,January,February and March 2018 to evaluate the potential human health risk.Chemical analyses were carried out by using ICP-SF-MS(Inductively Coupled Plasma-Sector Field Mass Spectrometry,Thermo Scientific Element II).The trace metals were detected at various concentrations in all the analyzed samples.Pb,Mn and Fe concentrations exceeded the European Union acceptable maximum limits for water intended for human consumption in 4.76%,28.57%and 61.90%of the groundwater samples,respectively and in 0%,50%and 100%of the surface water samples,respectively.As,Cr,Cu and Ni concentrations exceeded the recommended lower sediment quality guideline values in 33.33%,50%,83.33%and 83.33%of the surface sediment samples,respectively.All those elevated trace metal concentrations in the groundwater,surface water and sediments represent a risk for the health of local population as well as for aquatic organisms.
文摘Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxygen demand, salinity of the sea surface microlayer and subsurface water. Apparent enrichment mechanism and diurnal variation have been revealed for dissolved trace metals in the microlayer in Daya Bay. The more dissolved organic matter was enriched in the sea surface microlayer, the more dissolved trace metals were enriched in the layer. The organic matter played an important role in the enrichment process. The diurnal variations of dissolved trace metals showed that their concentration was apparently inversely related to the tide activity that the concentration was low during rising tide, but high during falling tide. The behavior of dissolved trace metals expressed by the diurnal variation was clearly opposite to that of salinity.
基金This paper is supported by the China Geological Survey(No200314200021).
文摘To examine the degree of contamination in the Dongting Lake （洞庭湖） area, surface sediments samples were collected and investigated in this study. This lake lies in the south of the middle Yangtze River, and it is the second largest fresh-water lake in China. These samples were analyzed for the concentrations of the following 6 elements （CA, Cr, Cn, Ni, Ph and Zn） collected from 46 locations. The index of geo-accumulation was used to assess the degree of contamination of the total trace elements and the assessments showed that the contamination of Cd reached strong to very strong pollution. The speciation of metal in sediments was analyzed using the modified BCR （Community Bureau of Reference） sequential extraction technology and the water column-contamination risk was evaluted by the calculated contamination factors. The results of the speciation of metal indicated that more easily mobilized forms （exchangeable, water soluble, and acid soluble） were predominant for CA, especially in the samples from East Dongting Lake. In contrast, the largest amount of lead was associated with the iron and manganese oxide fractions, and Cn, Zn, Cr, and Ni analyzed were mainly distributed in residual phase at an average percentage higher than 60 % of the total metals. The potential risk to the lake＇s water contamination was the highest in East Dongting Lake based on the calculated contamination factors. The assessment results using geo-accamnlation index were compared with the information obtained by trace metal speciation and both results were consistent.
基金Project supported by the France-China. Advanced Research Programme, France (No. PRA E03-03) and Natural ScienceFoundation of Guangdong Province (No. 032255).
文摘Physicochemical properties, total and DTPA （diethylenetriaminepentaacetic acid）-extractable Cu, Zn, Pb and Cd contents, microbial biomass carbon （C） content and the organic C mineralization rate of the soils in a long-term trace metal-contaminated paddy region of Guangdong, China were determined to assess the sensitivity of microbial indices to moderately metal-contaminated paddy soils. The mean contents of total Cu, Zn, Pb and Cd were 251, 250, 171, and 2.4 mg kg^-1 respectively. DTPA-extractable metals were correlated positively and significantly with total metals, CEC, and organic C （except for DTPA-extractable Cd）, while they were negatively and highly significantly correlated with pH, totall Fe and Mn. Metal stress resulted in relatively low ratios of microbial biomass C to organic C and in remarkable inhibition of the microbial metabolic quotient and C mineralization rate, which eventually led to increases in soil organic C and C/N. Moreover, microbial respiratory activity showed a stronger correlation to DTPA-extractable metals than to total metal content. Likewise, in the acid paddy soils some “linked” microbial activity indices, such as metabolic quotient and ratios of basal respiration to organic C, especially during initial incubation, were found to be more sensitive indicators of soil trace metal contamination than microbial biomass C or basal respiration alone.
基金Under the auspices of Science Foundation of Fujian Province (No. D0510025)National Natural Science Foundation of China (No. 40606012)
文摘Four short cores were obtained from the coastal wetland of the Quanzhou Bay,Fujian Province,China,and sediment samples were analyzed with a Mastersizer 2000 for grain size analysis and an Inductively Coupled Plasma Atomic Emission Spectrometer(ICP-AES) for trace metal analysis. The results of grain size analysis show that the wetland sediments are mainly composed of silt and clay,and the distribution of median grain size is affected by human activities obviously. The results of trace metal analysis show that the wetland sediments mainly originate from terrestrial materials in the Luoyangjiang River estuary and from contaminants discharged from adjacent rivers in Shuitou area. The heavy metal contamination in Luoyangjiang estuary decreased from 1984 to 2004 due to industry transformation,but has increased since 2004 because of the discharge of contaminants to the Jinjiang River and much living sewerage to the bay,and a large number of trace metals are trapped within Spartina alterniflora marsh.
基金Supported by the Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Fisheries Research Institute(No.201211)the National Natural Science Foundation of China(No.41206120)
文摘Selected trace metals （Pb, Cd, Cu, Hg） and arsenic in seawater and surface sediments of Laizhou Bay were determined, to evaluate their spatial distribution, pollution risk and potential ecological risk. Concentrations of the elements were 0.56-2.07, 0.14-0.38, 12.70-18.40, 0.014-0.094, and 1.13- 2.37 μg/L in the seawater and 8.94-32.2, 0.18-0.67, 4.51-30.5, 0.006-0.058, and 5.75-15.3 mg/kg in sediments for Pb, Cd, Cu, Hg and As, respectively. High concentrations of the trace metals and arsenic in seawater and surface sediments were generally observed near the fiver estuary. The pollution risk result of the elements showed that Cu was the prominent trace metal pollutant in seawater, followed by Hg, Pb, Cd and As. The metal complex pollution index in seawater was at a medium level. The most important trace metal pollutant in sediments was Cd, followed by As, Cu, Pb, and Hg. Our pollution assessment suggests that trace metal pollution in Laizhou Bay sediments was at a low level. The potential ecological risk was also low in surface sediment.
文摘A method based on the combination of coprecipitation with inductively coupledplasma atomic emission spectrometry (ICP-AES) was developed for the determination of impurities inhigh-purity sodium tungstate. Six elements (Co, Cu, Fe, Mn, Ni, and Pb) were coprecipitated bylanthanum hydroxide so as to be concentrated and separated from the tungsten matrix. Effects of somefactors on the recoveries of the analytes and on the residual amount of sodium tungstate wereinvestigated, and the optimum conditions for the coprecipitation were proposed. Matrix-matchingcalibration curve method was used for the analysis. It is shown that the elements mentioned abovecan be quantitatively recovered. The detection limits for Co, Cu, Fe, Mn, Ni, and Pb are 0.07, 0.4,0.2, 0.1, 0.6, and 1.3 μg·g^(-1), respectively. The recoveries vary from 92.5% to 108%, and therelative standard deviations (RSDs) are in the range of 3.1%-5.5%.
基金financially supported by the Key Construction Program of the National 985 Project,Tianjin University,Chinathe National Key R and D Program of China (2016YFA0601000)
文摘This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-MS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEM–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent DOM component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and Mg were significantly higher than Ca,Fe,Mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.
文摘The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The mean enrichment factors ([Xi]microlayer/[Xi ]15cm in depth) mostly ranged between 1 . 0 and 2. 0. The dissolved forms were the major forms of the components measured, the enrichment of dissolved organic matter and suspended particles could lead to the changes in the total amount and speciation of nutrients and trace metals. No correlation was observed between sample concentrations, speciation, enrichment factors and sample locations. However, some evidence shows that these parameters are correlated with sea state, indicating the complexity and dynamic nature of the sea surface microlayer.
文摘AIM: To assess whether trace metal concentrations (which influence metabolism as both essential and non-essential elements) are increased or decreased in cancerous tissues and to understand the precise role of these metals in carcinogenesis. METHODS: Concentrations of trace metals including Cd, Ni, Cu, Zn, Fe, Mg and Ca in both cancerous and non- cancerous stomach tissue samples were determined by atomic absorption spectrometry (AAS). Tissue samples were digested using microwave energy. Slotted tube atom trap was used to improve the sensitivity of copper and cadmium in flame AAS determinations. RESULTS: From the obtained data in this study, the concentrations of nickel, copper and iron in the cancerous human stomach were found to be significantly higher than those in the non-cancerous tissues, by using t-test for the paired samples. Furthermore, the average calcium concentrations in the cancerous stomach tissue samples were found to be significantly lower than those in the non-cancerous stomach tissue samples by using t-test. Exceedingly high Zn concentrations (207-826 mg/ kg) were found in two paired stomach tissue samples from both cancerous and non-cancerous parts. CONCLUSION: In contrast to the literature data for Cu and Fe, the concentrations of copper, iron and nickel in cancerous tissue samples are higher than those in the non-cancerous samples. Furthermore, the Ca levels are lower in cancerous tissue samples than in non-cancerous tissue samples.
基金supported by the National Basic Research Program of China (973 Pro-gram) under grant No.2010CB951203the National 908 Program under grant No.GX908 (supplement)
文摘Historical records of metal inputs were studied by using a sediment core collected from a sand-rich mudflat in the Qinjiang River estuary, China. 210Pb chronology was used to reconstruct the fluxes of Hg, Cu, Pb, Zn, Cd, Cr and As to the core site during the last 86 years. Based on the constant initial concentration model, the sedimentation rates are 1.18 cm year-1 in the top 30 cm sandy layer and 0.92 cm year-1 in the muddy bottom layer. To compensate for grain-size and mineralogy effects on metal concentra-tions, aluminum was used as the normalizing element. The enrichment factors (EF) indicate that the natural inputs had prevailed up to the early 1980s. After this period, the intensity of human activities has resulted in continual increasing trend of metals towards the surface. Recent sediment samples from the Qinjiang River estuary are found moderately enriched by Cd (EF>1.5) and slightly enriched by other metals (EF<1.5). Considering that the drainage area of the Qinjiang River is mostly agricultural land, the increased Cd may be due to the usage of fertilizers and pesticides in agricultural activities and the combustion of fossil fuels.
文摘The long-term seepage of hydrocarbons, either as macroseepage or microseepage, can set up near-surface oxidation reduction zones that favor the development of a diverse array of chemical and mineralogical changes. The bacterial oxidation of light hydrocarbons can directly or indirectly bring about significant changes in the values of pH and Eh of the surrounding environment, thereby also changing the stability fields of the different mineral species present in that environment. The paper reports the role of hydrocarbon microseepage in surface alterations of trace metal concentrations. In this study trace metal alterations were mapped that appear to be associated with hydrocarbon microseepages in the oil/ gas fields. A total of 50 soil samples were collected near oil and gas fields of the Tatipaka and Pasarlapudi areas of the Krishna Godavari Basin, Andhra Pradesh. The soil samples were collected from a depth of 2-2.5 m. The paper reports the chemical alterations associated with trace metals in soils that are related to hydrocarbon microseepages above some of the major oil and gas fields of this petroliferous region. Trace metals, such as scandium （Sc）, vanadium （V）, chromium （Cr）, cobalt （Co）, nickel （Ni）, copper （Cu）, zinc （Zn）, barium （Ba） and strontium （Sr）, in soil samples were analyzed using inductively coupled plasma- mass spectrometry （ICP-MS）. The concentrations of Sc （8 to 40 mg/kg）, V （197 to 489 mg/kg）, Cr （106 to 287 mg/kg）, Co （31 to 52 mg/kg）, Ni （65 to 110 mg/kg）, Cu （88 to 131 mg/kg）, Zn （88 to 471 mg/kg）, Ba （263 to 3,091 mg/kg） and Sr （119 to 218 mg/kg） were obtained. It was observed that the concentrations of trace elements were tremendously increased when they were compared with their normal concentrations in soils. The analysis of adsorbed soil gas showed the presence of high concentrations of YC2＋ （C2H6, C3H8 and n-C4H10） ranging from 7 to 222 μg/kg respectively. Integrated studies of trace elements over adsorbed light gaseous hydrocarbons （2C2＋） anomalies showed good correlation with the existing oil and gas wells. The carbon isotopic composition of δ13C1 of the samples ranges between -36.6%o to -22.7‰ （Pee Dee Belemnite） values indicate thermogenic origin, which presents convincing evidence that the adsorbed soil gases collected from these sediments are of catagenetic origin. The increase in the concentrations of trace metals near oil/gas producing areas, suggests a soil chemical change to a reducing environment, presumably due to the influence of hydrocarbon microseepage, which could be applied with other geoscientific data to identify areas of future hydrocarbon exploration in frontier areas.
文摘Objective To determine the metal contents of lichen species from East Black Sea region of Turkey for investigation of trace metal pollution sourced traffic. Methods The levels of copper, cadmium, lead, zinc, manganese, iron, chromium, nickel, cobalt, palladium in lichen samples collected from East Black Sea region of Turkey were determined by flame and graphite furnace atomic absorption spectrometry after microwave digestion method. The accuracy of the method was corrected by standard reference material （NIST SRM IAEA-336 Lichen）. Results The contents of invest!gated trace metals in lichen samples were 7.19-22.4 μg/g for copper, 0.10-0.64 μg/g for cadmium, 4.03-44.6 μg/g for lead, 14.5-41.8 μg/g for zinc, 25.8-208 μg/g for manganese, 331-436 μg/g for iron, 1.20-3.01 μg/g for chromium, 1.48-3.90 μg/g for nickel, 0.20-3.55 μg/g for cobalt, 0.11-0.64 μg/g for palladium. The results were compared with the literature values. Conclusion Some lichen species such as Xanthoparmelia conspersa, Xanthoria calcicola, Peltigera membranacea, and Physcia adscendens are accumulated trace metals at a high ratio.
文摘AIM: To investigate the oxidative-stress-related changes in rats with portal hypertension with particular emphasis on nitric oxide (NO) and trace metals. METHODS: Cirrhosis was induced by partial portal vein ligation (PVL) in Wistar rats. The lipid peroxidation marker (malondialdehyde, MDA), antioxidant defense enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and agents known to have antioxidant features including nitric oxide (NO), zinc (Zn), copper (Cu) were determined both in serum and in liver tissue at 4 wk after surgery in PVL and sham-operated rats. Portal pressure of all experimental animals was measured. MDA was detected by thiobarbituric acid reactivity assay.SOD activity was determined by inhibition of nitroblue tetrazolium reduction with xanthine/xanthine oxidase used asa superoxide generator. CAT activity was determined by the breakdown of hydrogen peroxide. GSH concentrations were measured by using metaphosphoric acid for protein precipitation and 5'-5'-dithio-bis-2-nitrobenzoic acid for color development. NO was detected by the Griess method after reduction of nitrate to nitrite with nitrate reductase, and the concentrations of Zn and Cu were measured by a Shimadzu 680 AA atomic absorption spectrometer. Histopathological confirmation was done under light microscope. Statistical analyses were done by Student's t-test, and significance of the difference was tested by the unpaired Mann-Whitney test. P＜0.05 was considered statistically significant.RESULTS: Histopathological studies confirmed PVL induced cirrhotic changes. There was a statistically significant difference in portal pressure between PVL and control groups (P＜0.001). The results showed significant increases in the levels of MDA and NO in both tissue and serum (P＜0.05 and P＜0.001, respectively in tissue; P＜0.001 for each in serum), and Zn only in tissue (P＜0.001)in rats with PVL compared with sham-operated rats. Besides, PVL rats exhibited reduced plasma and tissue GSH, CAT, SOD (P＜0.001 for each). Serum and tissue Cu concentration did not change.CONCLUSION: Our findings suggest that PVL in rats induces important biochemical and molecular changes related to oxidative stress in the liver.
基金the Chinese Academy of Sciences and The World Academy of Sciences for sponsoring this research through the CAS-TWAS President’s Fellowship program
文摘This study is aimed at determining the level of environmental degradation as well as the concentration of trace elements in soil and stream sediments in order to evaluate the environmental impact of the mining operation.Twenty-five(25) soils and ten(10) stream sediment samples were collected from the study area. The physicochemical parameters were determined using appropriate instrumentation with the aid of a digital p H meter(Milwaukee meter) to measure the p H and electrical conductivity, total dissolved solids, moisture content and loss on ignition of the soil and stream sediment samples. The p H of the soil sample ranged from(6.10 to 7.19); Electrical conductivity ranged from(21.3 to 279.4 μS/cm), moisture content varied from(0.60% to 7.20%), and the LOI ranged from(2.03% to 18.62%). The results of the analysis showed that the concentrations of the trace elements in the soils and stream sediment samples were slightly higher than the background values. Plots of the trace elements in stream sediment samples show moderate, consistent decrease downstream except at points where there was mine water discharge into the main river. The pollution levels of heavy metals were examined in stream sediment and soil samples using different assessable indices, such as the enrichment factor, which showed significant-moderate enrichment for Cr, Th, Nb, Zn, Pb, Y and Zr and the geoaccumulation index, which showed practically moderate contamination with Cr, Ni and Sr based on regional background reference values. Geo-accumulation index and contamination index for soils and stream sediment revealed uncontaminated to moderate contamination. Likewise,elements with moderate contamination were Cr, Ni and Sr.The Pearson correlation showed that there were significant positive associations among selected metals in soil and stream sediment samples.
基金Supported by the National Basic Research Program of China(973 Program)(No.2013CB956103)the National Science Foundation of China(Nos.41306109,41476038)
文摘Coral bleaching has generally been recognized as the main reason for tropical coral reef degradation, but there are few long-term records of coral bleaching events. In this study, trace metals including chromium(Cr), copper(Cu), molybdenum(Mo), manganese(Mn), lead(Pb), tin(Sn), titanium(Ti), vanadium(V), and yttrium(Y), were analyzed in two Porites corals collected from Meiji Reef in the tropical South China Sea(SCS) to assess differences in trace metal concentrations in bleached compared with unbleached coral growth bands. Ti, V, Cr, and Mo generally showed irregular fluctuations in both corals. Bleached layers contained high concentrations of Mn, Cu, Sn, and Pb. Unbleached layers showed moderately high concentrations of Mn and Cu only. The different distribution of trace metals in Porites may be attributable to different selectivity on the basis of vital utility or toxicity. Ti, V, Cr, and Mo are discriminated against by both coral polyps and zooxanthellae, but Mn, Cu, Sn, and Pb are accumulated by zooxanthellae and only Mn and Cu are accumulated by polyps as essential elements. The marked increase in Cu, Mn, Pb, and Sn are associated with bleaching processes, including mucus secretion, tissue retraction, and zooxanthellae expulsion and occlusion. Variation in these trace elements within the coral skeleton can be used as potential tracers of short-lived bleaching events.
