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Composition of Triacylglycerols in Fats of Cow and Goat Milk Produced in Four Zones of Mexico
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作者 Rey Gutié rrez Tolentino +14 位作者 Salvador Vega y Leó n Mario Noa Pé rez Marta Coronado Herrera Acacia Ramí rez Ayala José Jesús Pé rez González Beatriz Schettino Bermúdez Rutilio Ortiz Salinas Marcela Vazquez Francisca Juan Gabriel Rivera Martí nez 《Food and Nutrition Sciences》 2015年第6期555-561,共7页
The study of the triacylglycerols (TAG) by gas chromatography (GC) using capillary columns is an efficient technique for the determination of some characteristics of quality of fats and oils. The objective of the pres... The study of the triacylglycerols (TAG) by gas chromatography (GC) using capillary columns is an efficient technique for the determination of some characteristics of quality of fats and oils. The objective of the present study was to determine by GC the content of TAG present in fat of cow and goat milk produced in four zones of Mexico. According to criteria established in Mexican Standardization, 25 samples were obtained of 1 L of ultra-pasteurized cow milk (UHT) and 27 and 48 of raw cow and goat milk, respectively. The fat was extracted from all of the milk samples by detergent solution, and was stored at –20℃ until its analysis. The chromatographic conditions made it possible to identify and quantify TAG of 28 to 54 numbers of carbons, which were analyzed under descriptive and inferential statistical techniques. For the cow milk fat, the statistical analyses indicated significant difference (p < 0.05) in the TAG C34, C50 and C52, and for the goat milk fat in the TAG of C36 to C52. No equality of means was found among the TAG of the cow and goat milk fat. This study offers an advance in the characterization of the TAG present in the cow and goat milk fat produced in Mexico. 展开更多
关键词 COW MILK GOAT MILK Mexico triacylglycerols
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Rapid semi-quantification of triacylglycerols,phosphatidylcholines,and free fatty acids in the rice bran of one grain
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作者 Junko Nagamoto Ai Sawazaki +5 位作者 Motonori Miyago Bungo Shirouchi Mitsukazu Sakata TONG Litao Toshihiro Kumamaru Masao Sato 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第5期977-981,共5页
We developed a microplate assay method for determining the contents of triacylglycerols(TAGs), phosphatidylcholines(PCs), and free fatty acids(FFAs) in the rice bran of one grain using enzymatic reactions. In th... We developed a microplate assay method for determining the contents of triacylglycerols(TAGs), phosphatidylcholines(PCs), and free fatty acids(FFAs) in the rice bran of one grain using enzymatic reactions. In this method, enzymes from commercially available kits were used. Optimum reaction conditions were established. It was found that Nonidet P-40 was the optimal among the three surfactants used(Triton X-100, Tween 40, and Nonidet P-40) when lipid was dissolved in a reaction solution. Using this method, it was possible to quantify TAGs, PCs, and FFAs in concentration ranges of 7–150, 5–70, and 8–200 mg L-(–1), respectively. Furthermore, when the TAG contents in the rice bran were measured, the values closely corresponded to those obtained by extracting from large amounts of rice bran. However, sufficient data on the PC and FFA contents in rice bran are not available for valid comparisons. Although this method can accurately quantify the TAG contents in the rice bran of one grain, the accuracy of the PC and FFA contents has not been verified. Hence, future study is necessary. 展开更多
关键词 rice bran triacylglycerols phosphatidylcholines free fatty acids surfactants
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Impact of plant polyphenols on triacylglycerols and phospholipids in rat liver fed with high fat diet
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作者 Huifang Ma Ya Xie +8 位作者 Chen Yang Fang Wei Chao Hu Zongyuan Wu Zong Yang Xin Lyu Xuyan Dong Hong Chen Fenghong Huang 《Oil Crop Science》 2017年第4期251-264,共14页
The effect of plant (rapeseed and grape seed) polyphenols on lipid metabolism of rat fed with high fat diet was studied. 30 rats were divided into 5 groups including group Z (basal diet), C (high fat diet without plan... The effect of plant (rapeseed and grape seed) polyphenols on lipid metabolism of rat fed with high fat diet was studied. 30 rats were divided into 5 groups including group Z (basal diet), C (high fat diet without plant polyphenol), S (high fat diet with rapeseed polyphenols), R (high fat diet with grape seed polyphenols) and F (combined with rapeseed and grape seed polyphenols). Levels of lipids including TAGs and PLs in rats were investigated by shotgun-electrospray ionization tandem mass spectrometry (shotgun-ESI-MS). Results showed that high fat diet would give rise to the total TAGs and decreased total phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) (p < 0.05) in group C, S, R and F, compared with group Z. Results also showed that supplementary with rapeseed polyphenols or grape seed polyphenols reduced total TAGs and increased total PC, PE and PS (p < 0.05) in group S, R and F, compared with group C. Grape seed polyphenols and rapeseed polyphenols had certain regulatory effect on metabolism diseases such as fatty liver. 展开更多
关键词 triacylglycerols PHOSPHOLIPIDS shotgun-ESI-MS GRAPE seed POLYPHENOLS RAPESEED POLYPHENOLS
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Fast determination and quantification of triacylglycerols in plant oil by direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS)
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作者 Xin Lv Fang Wei Hong Chen 《Oil Crop Science》 2019年第4期267-274,共8页
Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes ... Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes a relatively long time during chromatographic separation.Therefore,in this study,a method about fast determination and quantification of TGs in plant oil based on MALDI-TOF-MS(matrix-assisted laser desorption ionization time-of-flight mass spectrometry)was established.OCNT(oxidized carbon nanotube)and CNT(carbon nanotube)were firstly used as matrix of MALDI-TOF-MS for TGs identification.Satisfyingly,OCNT showed good ionization efficiency,especially when used with CHCA(α-Cyano-4-hydroxy cinnamic acid).Sandwich method was optimized to solve the problem that how matrix and sample were deposited to the plate well and to improve ionization efficiency.Nine calibration curves were established for TGs quantification analysis in plant oil.To verify the accuracy of this method,relative content(%)of fatty acids(FAs)calculated from TGs in peanut oil,rapeseed oil,and soybean oil,respectively,by MALDI-TOF-MS were compared with that calculated from FAMEs(fatty acid methyl esters)by gas chromatography.In general,the results were relatively consistent,proving that this method was convenient,fast and accurate for the determination of main TG molecular species in plant oil. 展开更多
关键词 MATRIX MALDI-TOF-MS triacylglycerols PLANT OIL
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Isoform-and cell-state-specific APOE homeostasis and function 被引量:2
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作者 Karina Lindner Anne-Claude Gavin 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第11期2456-2466,共11页
Apolipoprotein E is the major lipid transporter in the brain and an important player in neuron-astrocyte metabolic coupling.It ensures the survival of neurons under stressful conditions and hyperactivity by nourishing... Apolipoprotein E is the major lipid transporter in the brain and an important player in neuron-astrocyte metabolic coupling.It ensures the survival of neurons under stressful conditions and hyperactivity by nourishing and detoxifying them.Apolipoprotein E polymorphism,combined with environmental stresses and/or age-related alterations,influences the risk of developing late-onset Alzheimer’s disease.In this review,we discuss our current knowledge of how apolipoprotein E homeostasis,i.e.its synthesis,secretion,degradation,and lipidation,is affected in Alzheimer’s disease. 展开更多
关键词 Alzheimer’s disease apolipoprotein E autophagy CHOLESTEROL lipid detoxification lipid transport lysosomal failure metabolic impairment TRIACYLGLYCEROL
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Chemical Composition of Pellonula leonensis Fish Oils from the Congo River Obtained by Soxhlet Extraction
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作者 Bob Wilfrid Loumouamou Léa Béatrice Mambou Gadet Michel Dzondo 《Open Journal of Applied Sciences》 2024年第8期2112-2123,共12页
The chemical composition of Pellonula leonensis fish oils from the Congo River (at Boko city) was carried out. The fatty acids were determined by gas chromatography. The Sn-2 position of fatty acids on glycerol was ca... The chemical composition of Pellonula leonensis fish oils from the Congo River (at Boko city) was carried out. The fatty acids were determined by gas chromatography. The Sn-2 position of fatty acids on glycerol was carried out by the ISO 6800 standard. The separation of the compounds (as free fatty acids, monoglycerides, diglycerides, triglycerides, sterols and methyl esters, etc.) was carried out by HPLC using gel permeation with refractometric detection. The phospholipid composition was by HPLC with an evaporative light scattering detector. We obtained oil contents of 32.10 (±0.46)%. The major fatty acids were Palmitic acid (27.41%) and oleic acid (24.23%). The SFA were of 44.50%. The MUFA represent 32.54% and PUFA 22.60%. Regarding the Sn-2 position on glycerol, 48.0% were by SFA and 51.3% were by unsaturated fatty acids. Among them 34.2% of fatty acids were 17.5% oleic acid molecules, 3.3% DHA molecules and 2.1% EPA molecules. Free fatty acids have contents of more than 62%, Diglycerides 16.63% and triglycerides 20.46%. Seven different phospholipids were identified, namely: Phosphatidylglycerol (PG), Phosphatidylethanolamine (PE), Phosphatidylinositol (PI), Phosphatidylcholine (PC), Sphingomyelin (SM), Lyso Phosphatitdylcholine (LPC) and Lyso Phosphatidylethanolamine (LPE). According to the high levels in position 2 of the glycerol of palmitic acid, the consumption of Pellonula leonensis fish could be moderate. 展开更多
关键词 Pellonula leonensis Fatty Acids Sn-2 Position TRIACYLGLYCEROL Phospholopids
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Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii 被引量:6
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作者 Xiao-dong DENG Jia-jia CAI Xiao-wen FEI 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第12期1121-1131,共11页
Lipid biosynthesis is essential for eukaryotic cells, but the mechanisms of the process in microalgae remain poorly understood. Phosphatidic acid phosphohydrolase or 3-sn-phosphatidate phosphohydrolase(PAP) catalyzes ... Lipid biosynthesis is essential for eukaryotic cells, but the mechanisms of the process in microalgae remain poorly understood. Phosphatidic acid phosphohydrolase or 3-sn-phosphatidate phosphohydrolase(PAP) catalyzes the dephosphorylation of phosphatidic acid to form diacylglycerols and inorganic orthophosphates. This reaction is integral in the synthesis of triacylglycerols. In this study, the mRNA level of the PAP isoform CrPAP2 in a species of Chlamydomonas was found to increase in nitrogen-free conditions. Silencing of the CrPAP2 gene using RNA interference resulted in the decline of lipid content by 2.4%–17.4%. By contrast, over-expression of the CrPAP2 gene resulted in an increase in lipid content by 7.5%–21.8%. These observations indicate that regulation of the CrPAP2 gene can control the lipid content of the algal cells. In vitro CrPAP2 enzyme activity assay indicated that the cloned CrPAP2 gene exhibited biological activities. 展开更多
关键词 Phosphatidate phosphohydrolase 2 Triacylglycerol biosynthesis RNAI Chlamydomonas reinhardtii Nitrogen deprivation OVER-EXPRESSION
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Synthesis of symmetrical medium-and long-chain triacylglycerols rich in arachidonic acid at sn-2 position for infant formula
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作者 Xuan Jiang Jianhua Huang +6 位作者 Yaru Li Ye Wen Shibin Wu Xiuli Xu Zhonghao Chao Hong Zhang Xiaoqiang Zou 《Food Bioscience》 SCIE 2022年第1期386-392,共7页
Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by... Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula. 展开更多
关键词 Arachidonic acid sn-2 monoacylglycerol LIPASE Medium-and long-chain triacylglycerol Infant formula
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Altered Levels of Blood Glucose and Serum Lipids in Sudanese Patients with Ovarian Cancer
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作者 Maysoon A. Hassaan Atif H. Khirelsied +1 位作者 Tagelsir M. Ali Ahmed A. Agab-Aldour 《Open Journal of Obstetrics and Gynecology》 CAS 2023年第5期896-906,共11页
Background: The etiology of ovarian cancer is not well-understood;numerous metabolomics profiling, epidemiological, and hospital-based case control studies have associated abnormal levels of blood glucose and serum li... Background: The etiology of ovarian cancer is not well-understood;numerous metabolomics profiling, epidemiological, and hospital-based case control studies have associated abnormal levels of blood glucose and serum lipids with the risk and the prognosis of various types of cancers including ovarian cancer. The association between the risk of the incidence of ovarian cancer and the alterations in the levels of blood glucose and serum lipids is not well defined. Objective: In this study we aimed to compare the levels of blood glucose, triacylglycerols, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in patients with different stages of ovarian cancer and healthy controls to determine how they relate to the risk and prognosis of ovarian cancer. Methodology: In a case-control cross sectional study, we enrolled ninety-nine Sudanese women, diagnosed with ovarian cancer but had not received any kind of treatment as the study group, and a control group of forty-one age-matched, apparently healthy women. The patients were classified according to the International Federation of Obstetricians and Gynecologists staging system into two groups: early stages (stage I & II) and late stages (stages III & IV). Blood glucose and serum lipids;triacylglycerols, total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol were determined by enzymatic colorimetric methods using commercially available analytical kits. The IBM SPSS version 20 software was used for statistical analysis. A Mann-Whitney U test was used for comparison of the median concentrations of blood glucose, triacylglycerols, total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol in the study groups. Logistic regression model was used to estimate the relative risk of ovarian cancer in relation to levels of blood glucose and serum lipids. P value of 0.05 was considered significant. Results: Our data indicated significantly higher levels of blood glucose (p < 0.001), triacylglycerols (p = 0.002), and low-density lipoprotein cholesterol (p < 0.001), and lower levels of high-density lipoprotein cholesterol (p = 0.023), in ovarian cancer patients compared to the control subjects. No significant difference was found in the levels of blood glucose or any of the serum lipids between patients in the early stages (stage I & II) and those in late stages (stage III & IV) of ovarian cancer. The logistic regression analysis indicated significant association between the elevated levels of the blood glucose, triacylglycerols and low-density lipoprotein cholesterol and the risk of the ovarian cancer. Conclusion: We conclude that the levels of blood glucose, triacylglycerols, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol differ significantly between ovarian cancer patients and the healthy control subjects. The risk of ovarian cancer was positively associated with the levels of blood glucose, triacylglycerols and low-density lipoprotein cholesterol, and negatively associated with levels of high-density lipoprotein cholesterol. Therefore, determination of blood glucose and serum lipids, particularly, triacylglycerols, low-density lipoprotein cholesterol may be helpful as diagnostic indicators of ovarian cancer (OC). 展开更多
关键词 Blood Glucose CHOLESTEROL Ovarian Cancer Serum Lipids TRIACYLGLYCEROL
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Lipase-Catalyzed Synthesis of Sn-2 Palmitate: A Review 被引量:7
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作者 Wei Wei Cong Sun +4 位作者 Xiaosan Wang Qingzhe Jin Xuebing Xu Casimir CAkoh Xingguo Wang 《Engineering》 SCIE EI 2020年第4期406-414,共9页
Human milk fat(HMF)is an important source of nutrients and energy for infants.Triacylglycerols(TAGs)account for about 98%of HMF and have a unique molecular structure.HMF is highly enriched in palmitic acid(PA)at the s... Human milk fat(HMF)is an important source of nutrients and energy for infants.Triacylglycerols(TAGs)account for about 98%of HMF and have a unique molecular structure.HMF is highly enriched in palmitic acid(PA)at the sn-2 position of the glycerol backbone(more than 70%)and in unsaturated fatty acids at the sn-1,3 position.The specific TAG structure in HMF plays a valuable function in infant growth.Sn-2 palmitate(mainly 1,3-dioleoyl-2-palmitoyl-glycerol)is one of the structured TAGs that is commonly supplemented into infant formula in order to enable it to present a similar structure to HMF.In this review,the development of the lipase-catalyzed synthesis of sn-2 palmitate over the last 25 years are summarized,with a focus on reaction schemes in a laboratory setting.Particular attention is also paid to the commercialized sn-1,3 regioselective lipases that are used in structured TAGs synthesis,to general methods of TAG analysis,and to successfully developed sn-2 palmitate products on the market.Prospects for the lipase-catalyzed synthesis of sn-2 palmitate are discussed. 展开更多
关键词 Structured triacylglycerols Palmitic acid Human milk fat substitutes Lipase-catalyzed reaction Sn-2 palmitate Quantitative analysis
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Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis 被引量:1
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作者 Yanxia Jia Weiqi Li 《Plant Diversity》 SCIE CAS CSCD 2018年第6期292-298,共7页
Storage oil breakdown plays an important role in the life cycle of many plants by providing the carbon skeletons that support seedling growth immediately following germination. 1-Butanol, a specific inhibitor of phosp... Storage oil breakdown plays an important role in the life cycle of many plants by providing the carbon skeletons that support seedling growth immediately following germination. 1-Butanol, a specific inhibitor of phospholipase D(PLD)-dependent production of the signalling molecule phosphatidic acid(PA), inhibited Arabidopsis seed germination. N-Acylethanolamines(NAEs), which have been shown to inhibits PLDa1 activity, have no effect on seed germination. However, mobilization profile of triacylglycerols(TAG) that induced by each compound has not been reported. To gain deeper insights into the mode of mobilization of TAG during NAE 12:0 or 1-butanol treatment, we conducted a detailed comparative analysis of the effect of NAE 12:0, DMSO, 1-butanol and tert-butanol on Arabidopsis seed germination and fatty acid composition, tert-butanol and DMSO served as the corresponding controls treatment respectively. Our data show that 1-butanol, but not the inactive tert-butanol isomer, inhibited Arabidopsis seed germination, which is accompanied by a with retardation of the mobilization of triacylglycerols(TAG). In contrast, NAE 12:0 did not affect mobilization of TAG, nor did it significantly delay seed germination as monitored by radicle and cotyledon emergence. 1-Butanol induced RNA degradation in seeds and seedlings. We speculate that the large-scale degradation of RNA under the induction of 1-butanol may lead to abnormal gene expression in genes necessary for seed germination, including the genes needed for the mobilization of oil bodies, and thus cause a delay of seed germination. To the best of our knowledge, we report for the first time that 1-butanol delays the mobilization of TAG. 展开更多
关键词 Germination 1-butanol N-ACYLETHANOLAMINES triacylglycerols Fatty acid
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Dietary Calcium Decreases Plasma Cholesterol Level only in Female but not in Male Hamster Fed a High Cholesterol Diet 被引量:1
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作者 MA Ka Ying LIANG Yin Tong +7 位作者 CHEN Jing Nan JIANG Yue KWAN Kin Ming PENG Cheng JIAO Rui ZUO Yuan Yuan HUANG Yu CHEN Zhen Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第4期392-398,共7页
Objective To investigate the effect of dietary calcium on plasma lipoprotein profile in castrated and ovariectomized hamsters. Methods Male, castrated, female and ovariectomized hamsters (n=36 each group) were rando... Objective To investigate the effect of dietary calcium on plasma lipoprotein profile in castrated and ovariectomized hamsters. Methods Male, castrated, female and ovariectomized hamsters (n=36 each group) were randomly divided into three sub-groups (n=12) and fed one of the three diets containing O, 2, and B g calcium per kg diet for a period of six weeks. Changes in plasma lipoprotein profile were monitored at the end of week O, 3 and 6. Results Plasma total cholesterol (TC), non-high density lipoprotein cholesterol (non-HDL-C), triacylglycerols (TG) and TC/HDL-C were decreased only in intact female and ovariectomized hamsters. In contrast, three levels of dietary calcium had no effect on lipoprotein profiles in both intact male and castrated hamsters. Conclusion Beneficial modification of lipoprotein profile by dietary calcium was gender-dependent at least in hamsters. 展开更多
关键词 Calcium CHOLESTEROL triacylglycerols Fecal sterols
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Advances on Current Techniques and Methodologies in Milk Lipidomics
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作者 Li Mo-han Rayhnigul Abdlla +6 位作者 Chen Jia-li Liu Yi-ming Zhang Xiu-min Cao Xue-yan Yang Mei Zheng Yan Yue Xi-qing 《Journal of Northeast Agricultural University(English Edition)》 CAS 2021年第4期25-37,共13页
Milk is a complex biological fluid containing lipids,proteins,carbohydrates and minerals,which are essential for infant growth.While the lipid portion constitutes only 3%-5%of the total milk composition,it accounts fo... Milk is a complex biological fluid containing lipids,proteins,carbohydrates and minerals,which are essential for infant growth.While the lipid portion constitutes only 3%-5%of the total milk composition,it accounts for over 50%of the infant’s daily energy intake.The dominant portion(approximately 98%)is in the form of triacylglycerols and polar lipids,such as glycerophospholipids and sphingolipids,forming minor components.Recently,with the development of lipidomics,important progresses have been made in milk lipidomics,and the identification and quantification of several milk lipids at the group and molecular species level has become a reality,thereby providing useful information for the infant formula industry.In this review,an overview of the separation of the main components of milk lipids was presented,including glycerolipids,phospholipids and sphingolipids.The analytical methods and strategies for milk lipidomics,including gas chromatography-mass spectrometry(MS),capillary electrophoresis MS,nuclear magnetic resonance,matrix-assisted laser desorption ionization-MS,electrospray ionization-MS,shotgun lipidomics and liquid chromatography-MS,were reviewed.Additionally,the bioinformatics of lipidomics for milk lipid determination,including lipid classification,lipid databases and lipid analysis software,were investigated.This review would aid future investigations of the nutrition of milk lipids and refined researches on formula milk powder. 展开更多
关键词 MILK LIPIDOMICS triacylglycerols GLYCEROPHOSPHOLIPIDS SPHINGOLIPIDS
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Anti-inflammatory and anti-thrombogenic effects of atorvastatin in acute ischemic stroke 被引量:20
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作者 Lianqiu Min Shuai Shao +4 位作者 Xiaoning Wu Lin Cong Ping Liu Haiping Zhao Yumin Luo 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第23期2144-2154,共11页
Atorvastatin decreases inflammation and thrombogenesis in patients with carotid artery plaque. Atorvastatin is administered to lower lipid levels, but its anti-inflammatory and anti-thrombogenic effects remain unclear... Atorvastatin decreases inflammation and thrombogenesis in patients with carotid artery plaque. Atorvastatin is administered to lower lipid levels, but its anti-inflammatory and anti-thrombogenic effects remain unclear. Eighty-nine patients from northeastern China with acute ischemic stroke caused by large-artery atherosclerosis were randomly divided into the study and control groups. All patients received routine treatment, including antiplatelet therapy, circulatory support, and symp- tomatic treatment. The study group (n = 43) also received daily atorvastatin 20 mg/d, and the control group (n = 46) received daily placebo pills containing glucose. After 4 weeks, the levels of C-reactive protein, fibrinogen, and D-dimer were significantly lower in the study group than in the control group. Decreases in the levels of C-reactive protein, fibrinogen, and D-dimer were not associated with de- creases in the levels of triacylglycerol and low-density lipoprotein cholesterol. These results suggest that atorvastatin reduces inflammation and thrombogenesis independent of its lipid-lowering effects in patients with acute ischemic stroke caused by large-artery atherosclerosis. 展开更多
关键词 neural regeneration brain injury ischemic strokeC-reactive protein FIBRINOGEN D-dimer inflammationlarge-artery atherosclerosis ATORVASTATIN THROMBUS TRIACYLGLYCEROL low-density lipo-protein cholesterol grants-supported paper neuroregeneration
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miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta 被引量:9
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作者 Liuan Ma Huiling Qiu +4 位作者 Zhi Chen Li Li Yan Zeng Jun Luo Deming Gou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2018年第4期868-877,共10页
Background: The goat(Caprahircus) is one of the most important livestock animals. Goat milk fat is an important component in the nutritional quality of goat milk. Growing evidence points to the critical roles of micro... Background: The goat(Caprahircus) is one of the most important livestock animals. Goat milk fat is an important component in the nutritional quality of goat milk. Growing evidence points to the critical roles of microRNAs(miRNAs) in lipid metabolism.Results: Using a highly sensitive method of S-poly(T) plus for miRNAs detection, we analyze the expression patterns of 715 miRNAs in goat mammary gland tissues at different stages of lactation. We observed that miR-25 expression had an inverse relationship with milk production. Overexpression of miR-25 significantly repressed triacylglycerol synthesis and lipid droplet accumulation. To explore the regulatory mechanism of miR-25 in milk lipid metabolism,we analyzed its putative target genes with bioinformatics analysis followed by 3′-UTR assays. Peroxisome proliferative activated receptor gamma coactivator 1 beta(PGC-1 beta), a key regulator of lipogenics was identified as a direct target of miR-25 with three specific sites within its 3′-UTR. In addition, miR-25 mimics in goat mammary epithelial cells reduced the expressions of genes involved in lipid metabolism.Conclusions: Taken together, our results show miR-25 is potentially involved in lipid metabolism and we reveal the function of the miR-25/PGC-1 beta regulatory axis during lactation. 展开更多
关键词 Goat mammary epithelial cells LIPID miR-25 PGC-1beta TRIACYLGLYCEROL
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Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang 被引量:2
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作者 Wenqiang Wang Hao Wen +6 位作者 Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu 《Food Science and Human Wellness》 SCIE 2022年第5期1201-1214,共14页
Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples ... Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic analysis.The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were PUFAs.We identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing.Ten key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid biosynthesis.Quantitative PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic data.Finally,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut. 展开更多
关键词 WALNUT Oil accumulation Triacylglycerol assembly Transcription factors Correlation analysis
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The Light Regime Effect on Triacylglycerol Accumulation of Isochrysis zhangjiangensis 被引量:1
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作者 FAN Xuran CAO Xupeng +2 位作者 CHU Yadong WU Peichun XUE Song 《Journal of Ocean University of China》 SCIE CAS CSCD 2019年第2期474-480,共7页
Stress state of microalgal cells is caused under unfavorable conditions such as disordered light regime and depleted nitrogen. The stress state can impair photosynthetic efficiency, inhibit cell growth and result in t... Stress state of microalgal cells is caused under unfavorable conditions such as disordered light regime and depleted nitrogen. The stress state can impair photosynthetic efficiency, inhibit cell growth and result in the accumulation of triacylglycerol(TAG) from protective mechanisms. Continuous light or nitrogen starvation was applied on microalgae and performed effectively on inducing TAG production. To evaluate the light regime effect on inducing TAG production, the effect of different light regimes on nitrogen-starved Isochrysis zhangjiangensis was investigated in this work. The continuous light and nitrogen starvation elevated TAG content of biomass by 73% and 193%, respectively. Furthermore, the TAG accumulation of I. zhangjiangensis cell under nitrogen starvation decreased under aggravated stress from continuous illumination. Our results demonstrated that culturing the cells with 14 L: 10 D light regime under nitrogen starvation is the optimal mode to achieve maximal accumulation of TAG. A recovery in light regime was necessary for I. zhangjiangensis cultivation. 展开更多
关键词 microalgal CULTIVATION TRIACYLGLYCEROL ACCUMULATION light REGIME NITROGEN STARVATION
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A Study on Triacylglycerol Composition and the Structure of High-Oleic Rapeseed Oil 被引量:3
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作者 Mei Guan Hong Chen +4 位作者 Xinghua Xiong Xin Lu Xun Li Fenghong Huang Chunyun Guan 《Engineering》 SCIE EI 2016年第2期258-262,共5页
The composition of fatty acids in triacylglycerides (TAGs) and their position on the glycerol backbone de- termine the nutritional value of vegetable oil. In this study, gas chromatography and high-performance liqui... The composition of fatty acids in triacylglycerides (TAGs) and their position on the glycerol backbone de- termine the nutritional value of vegetable oil. In this study, gas chromatography and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were used to analyze the compo- sition and distribution of fatty acids in TAGs of different rapeseed oils. Our results show the content of oleic acid in higb-oleic-acid rapeseed oil to be about 80%. In terms of the number of acyl carbon atoms (CN), TAGs with CN52-54 were most abundant, with a maximum concentration at CN54 (80%). The main type of TAG was oleic-oleic-oleic (OOO), accounting for 71.75%, while oleic-oleic-linoleic (OOL) accounted for ?.56%, oleic-oleic-linolenic (OOLn) accounted for 4.81%, and stearic-oleic-oleic (SO0) accounted for 4.74%. Oleic acid in high-oleic-acid rapeseed oil was distributed in the following order of preference: sn-2 〉 sn-1/3. In high-erucic-acid rapeseed oil, however, oleic acid was enriched at the sn-1/3. These data show that the content of oleic acid can be as high as about 80% in high-oleic-acid material. This finding suggests that high-oleic-acid rapeseed oil has high nutritional value. 展开更多
关键词 Brassica napus Fatty acid Triacylglycerol
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Diacylglycerol-enriched Oil from Hydrolysis of Soybean Oil with Rhizopus Oryzae Lipase Against High-fat Diet-induced Obesity in Mice 被引量:1
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作者 ZHANG Mengke SHI Bo +1 位作者 LI Chao CHENG Yongqiang 《Grain & Oil Science and Technology》 2018年第1期53-58,共6页
The present work aimed to investigate the impact of intake of diacylglycerol(DAG) from soybean oil on the reduction of fat accumulation and the long-term effects of dietary intake of DAG and triacylglycerol(TAG) with ... The present work aimed to investigate the impact of intake of diacylglycerol(DAG) from soybean oil on the reduction of fat accumulation and the long-term effects of dietary intake of DAG and triacylglycerol(TAG) with similar fatty acid compositions on the development of obesity. Kuming mice were used to compare the effects of low-dose TAG(2.5 g/kg BW), low-dose DAG(2.5 g/kg BW), high-dose TAG(10 g/kg BW), and high-dose DAG(10 g/kg BW) on the induced obesity. The results showed that the body weight and serum triglyceride concentration decreased significantly in both DAG-treated groups compared with the TAG-treated groups(P < 0.01, P < 0.05, respectively). However, the plasma glucose concentration was significantly lower in the DAG-treated groups than the TAG-treated groups(P < 0.05); the weight and morphology of the liver and kidney in DAG-treated groups were similar to those in the control group, there were no significant differences within each group. The present results indicated the anti-obesity and lipid-lowering effects of dietary DAG oil in mice and its potential use as a functional food for humans. 展开更多
关键词 DIACYLGLYCEROL TRIACYLGLYCEROL OBESITY Soybean oil MICE
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Genetic Manipulation of Non-Classic Oilseed Plants for Enhancement of Their Potential as a Biofactory for Triacylglycerol Production 被引量:3
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作者 Xiao-Yu Xu Hong-Kun Yang +2 位作者 Surinder P. Singh Peter J. Sharp Qing Liu 《Engineering》 2018年第4期523-533,共11页
Global demand for vegetable oil is anticipated to double by 2030. The current vegetable oil production platforms, including oil palm and temperate oilseeds, are unlikely to produce such an expansion. Therefore, the ex... Global demand for vegetable oil is anticipated to double by 2030. The current vegetable oil production platforms, including oil palm and temperate oilseeds, are unlikely to produce such an expansion. Therefore, the exploration of novel vegetable oil sources has become increasingly important in order to make up this future vegetable oil shortfall. Triacylglycerol (TAG), as the dominant form of vegetable oil, has recently attracted immense interest in terms of being produced in plant vegetative tissues via genetic engineering technologies. Multidiscipline-based "-omics" studies are increasingly enhancing our understanding of plant lipid biochemistry and metabolism. As a result, the identification of biochemical pathways and the annotation of key genes contributing to fatty acid biosynthesis and to lipid assembly and turnover have been effectively updated. In recent years, there has been a rapid development in the genetic enhancement of TAG accumulation in high-biomass plant vegetative tissues and oilseeds through the genetic manipulation of the key genes and regulators involved in TAG biosynthesis. In this review, current genetic engineering strategies ranging from single-gene manipulation to multigene stacking aimed at increasing plant biomass TAG accumulation are summarized. New directions and suggestions for plant oil production that may help to further alleviate the potential shortage of edible oil and biodiesel are discussed. 展开更多
关键词 Plant vegetable oil TRIACYLGLYCEROL Genetic engineering Edible oil BIODIESEL
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