The issue of the extremely imbalanced gender ratio in preschool teachers has received widespread attention,and there are few studies on teacher-child verbal interaction behavior based on gender differences in preschoo...The issue of the extremely imbalanced gender ratio in preschool teachers has received widespread attention,and there are few studies on teacher-child verbal interaction behavior based on gender differences in preschool teachers.This article takes the“Little Light Bulb Is On”,a scientific exploration activity done by 5-6 years old kindergarten students as an example,and uses the improved Flanders Interaction Analysis System(iFIAS)as a tool to analyze the speech interaction behavior of male and female preschool teachers.The research results indicate that there are gender differences in teacher child language interaction between male and female teachers in terms of the atmosphere,the teaching structure,the teaching tendency,the way of raising questions,and the overall trend of interaction.展开更多
Based on prior studies and a questionnaire survey,this paper is seeking to demonstrate that interactive activities will facilitate EFL learners' social strategy awareness and use,and thus enhance their linguistic ...Based on prior studies and a questionnaire survey,this paper is seeking to demonstrate that interactive activities will facilitate EFL learners' social strategy awareness and use,and thus enhance their linguistic development.A sample lesson plan is also presented in this paper to illustrate that social strategy awareness and use can be improved through interactive learning activities.展开更多
In this paper, we conduct research on the interactive training pattern and the applications on the modern emergency rescue training activities. In emergency rescue training linkage the ecological communities, importan...In this paper, we conduct research on the interactive training pattern and the applications on the modern emergency rescue training activities. In emergency rescue training linkage the ecological communities, important is not gathered themselves together, and not the size of the momentum and the strength that is not the amount of emergency rescue training linkage performance, important is the ecological communities each organic elements in the function of the cohesion, lies in the relationship between the organic elements in ecological communities, the construction and the development is the specific ecological communities in action elements of emergency rescue training, joint quality lies in the emergency rescue training. These contents are involved in the emergency rescue training linkage management model of concrete. Under this basis, we integrate the condition of the Hainan Medical School to propose the corresponding suggestions that will enhance the performance.展开更多
A novel thermodynamic model-the molecular interaction volume model （MIVM） which can be reduced to the Flory-Huggins equation of polymer solution was employed for the prediction of component activities in the ternary...A novel thermodynamic model-the molecular interaction volume model （MIVM） which can be reduced to the Flory-Huggins equation of polymer solution was employed for the prediction of component activities in the ternary molten aluminosilicate slag CaO-Al2O3-SiO2 at different temperatures. The results show that the predicted values of activity of CaO, Al2O3 and SiO2 are in reasonably agreement with experimental data in some ranges of their concentrations which are about x1 〈0.25 for CaO, x2=0.05-0.55 for Al2O3 and x3=0.03-0.85 for SiO2. This further shows that MIVM requires only two binary parameters for each sub-binary system to predict activities of all components in a multicomponent solution and is the superior alternative in a molten slag.展开更多
Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the...Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the foreign language.This paper will introduce some oral communicative activities that can motivate students to practice conversation skills and promote interaction.展开更多
The periphery of the Qinghai-Tibet Plateau is renowned for its susceptibility to landslides.However,the northwestern margin of this region,characterised by limited human activities and challenging transportation,remai...The periphery of the Qinghai-Tibet Plateau is renowned for its susceptibility to landslides.However,the northwestern margin of this region,characterised by limited human activities and challenging transportation,remains insufficiently explored concerning landslide occurrence and dispersion.With the planning and construction of the Xinjiang-Tibet Railway,a comprehensive investigation into disastrous landslides in this area is essential for effective disaster preparedness and mitigation strategies.By using the human-computer interaction interpretation approach,the authors established a landslide database encompassing 13003 landslides,collectively spanning an area of 3351.24 km^(2)(36°N-40°N,73°E-78°E).The database incorporates diverse topographical and environmental parameters,including regional elevation,slope angle,slope aspect,distance to faults,distance to roads,distance to rivers,annual precipitation,and stratum.The statistical characteristics of number and area of landslides,landslide number density(LND),and landslide area percentage(LAP)are analyzed.The authors found that a predominant concentration of landslide origins within high slope angle regions,with the highest incidence observed in intervals characterised by average slopes of 20°to 30°,maximum slope angle above 80°,along with orientations towards the north(N),northeast(NE),and southwest(SW).Additionally,elevations above 4.5 km,distance to rivers below 1 km,rainfall between 20-30 mm and 30-40 mm emerge as particularly susceptible to landslide development.The study area’s geological composition primarily comprises Mesozoic and Upper Paleozoic outcrops.Both fault and human engineering activities have different degrees of influence on landslide development.Furthermore,the significance of the landslide database,the relationship between landslide distribution and environmental factors,and the geometric and morphological characteristics of landslides are discussed.The landslide H/L ratios in the study area are mainly concentrated between 0.4 and 0.64.It means the landslides mobility in the region is relatively low,and the authors speculate that landslides in this region more possibly triggered by earthquakes or located in meizoseismal area.展开更多
Based on ECMWF objective analysis data, and with use of Batterworth bandpass-filtered skill and diagnostic analysis method, the interaction characteristics of low frequency wave and mean flow in midlatitudes during t...Based on ECMWF objective analysis data, and with use of Batterworth bandpass-filtered skill and diagnostic analysis method, the interaction characteristics of low frequency wave and mean flow in midlatitudes during the winter years of 1983 / 1984 and 1986 / 1987 have been studied in this paper. The main results point out the important role of the tropical convective activity on the above mentioned interaction process.展开更多
Because of the evolution of markets and technologies, prototyping concerns should be kept updated almost day by day. Moreover, user centered design moves the focus towards interaction issues. Prototyping activities ma...Because of the evolution of markets and technologies, prototyping concerns should be kept updated almost day by day. Moreover, user centered design moves the focus towards interaction issues. Prototyping activities matching such characteristics are already available, but they are not so diffused in the industrial domain. This is due to many reasons;an important one is that a rigorous classification of them is missing, as well as an effective helping tool for the selection of the best activities, given the design context. The research described in this paper aims at defining a new classification of prototyping activities, as well as at developing a selection algorithm to choose the best ones in an automatic way. These goals are pursued by defining a set of characteristics that allow describing accurately the prototyping activities. The resulting classification is made by five classes, based on eighteen characteristics. This classification is exploited by the first release of an algorithm for the selection of the best activities, chosen in order to satisfy design situations described thanks to a different set of eleven indices. Five experiences in the field have been used up to now as a starting point for validating the research outcomes.展开更多
Objective To study the effect of fleroxacin （FLRX） on biological properties of Bloom （BLM） helicase catalytic core （BLM 642-2290 helicase） in vitro and the molecular mechanism of interaction between the two mol...Objective To study the effect of fleroxacin （FLRX） on biological properties of Bloom （BLM） helicase catalytic core （BLM 642-2290 helicase） in vitro and the molecular mechanism of interaction between the two molecules. Methods DNA-binding and unwinding activities of BLM 642-1290 helicase were assayed by fluorescence polarization and gel retardation assay under conditions that the helicase was subjected to different concentrations of FLRX. Effect of FLRX on helicase ATPase activity was analyzed by phosphorus-free assay based on a colorimetric estimation of ATP hydrolysis-produced inorganic phosphate. Molecular mechanism of interaction between the two molecules was assayed by ultraviolet and fluorescence spectra. Results The DNA unwinding and ATPase activities of BLM 642-1290 helicase were inhibited whereas the DNA-binding activity was promoted in vitro. A BLM-FLRX complex was formed through one binding site, electrostatic and hydrophobic interaction force. Moreover, the intrinsic fluorescence of the helicase was quenched by FLRX as a result of non-radioactive energy transfer. The biological activity of helicase was affected by FLRX, which may be through an allosteric mechanism and stabilization of enzyme conformation in low helicase activity state, disruption of the coupling of ATP hydrolysis to unwinding, and blocking helicase translocation on DNA strands. Conclusion FLRX may affect the biological activities and conformation of BLM 642-1290 helicase, and DNA helicase may be used as a promising drug target for some diseases.展开更多
The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After...The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After reaction, tuffaceous slate became light colored and soft, and its mass density reduced. The amount of gold extracted from tuffaceous slate ranges widely, from 0 027 to 0 234 μg/g. Chlorine solution may activate appreciable amount of gold, and the gold migratory rate is high enough, from 50 70% to 92 30%, which reveals that sulphur and chlorine work together in solutions to accelerate gold activation and migration, and to realize gold mineralization in favorable places.展开更多
Gold catalysts have been reported as highly effective catalysts in various oxidation reactions.However,for chemoselective hydrogenation reactions,gold‐based catalysts normally show much lowercatalytic activity than p...Gold catalysts have been reported as highly effective catalysts in various oxidation reactions.However,for chemoselective hydrogenation reactions,gold‐based catalysts normally show much lowercatalytic activity than platinum group metals,even though their selectivities are excellent.Here,wereport that the chemoselective hydrogenation activity of 3‐nitrostyrene to 3‐vinylaniline overAu/TiO_(2)can be enhanced up to 3.3 times through the hydrogen reduction strategy.It is revealedthat strong metal‐support interaction,between gold nanoparticles(NPs)and TiO_(2)support,is introducedthrough hydrogen reduction,resulting in partial dispersion of reduced TiOx on the Au surface.The partially covered Au not only increases the perimeter of the interface between the gold NPs andthe support,but also benefits H_(2)activation.Reaction kinetic analysis and H_(2)‐D2 exchange reactionshow that H_(2)activation is the critical step in the hydrogenation of 3‐nitrostyrene to 3‐vinylaniline.Density functional theory calculations verify that hydrogen dissociation and hydrogen transfer arefavored at the interface of gold NPs and TiO_(2)over the hydrogen‐reduced Au/TiO_(2).This study providesinsights for fabricating highly active gold‐based catalysts for chemoselective hydrogenationreactions.展开更多
Axonal injury is a pathological hallmark of both head injury and inflammatory-mediated neurological disorders,including multiple sclerosis（Schirmer et al.,2013）.Such axonal disruptions and/or disconnections typicall...Axonal injury is a pathological hallmark of both head injury and inflammatory-mediated neurological disorders,including multiple sclerosis（Schirmer et al.,2013）.Such axonal disruptions and/or disconnections typically result in proximal axonal segments that remain in continuity with the neuronal somawhile losing contact with their distal targets.展开更多
From the organization of animal flocks to the emergence of swarming behaviors in bacterial suspension,populations of motile organisms at all scales display coherent collective motion.Recent studies showed that the ani...From the organization of animal flocks to the emergence of swarming behaviors in bacterial suspension,populations of motile organisms at all scales display coherent collective motion.Recent studies showed that the anisotropic interaction between active particles plays a key role in the phase behaviors.Here we investigate the collective behaviors of based-active Janus particles that experience an anisotropic interaction of which the orientation is opposite to the direction of active force by using Langevin dynamics simulations in two dimensional space.Interestingly,the system shows emergence of collective swarming states upon increasing the total area fraction of particles,which is not observed in systems without anisotropic interaction or activity.The threshold for emergence of swarming states decreases as particle activity or interaction strength increases.We have also performed basic kinetic analysis to reproduce the essential features of the simulation results.Our results demonstrate that anisotropic interactions at the individual level are sufficient to set homogeneous active particles into stable directed motion.展开更多
To gain insight into the function of AOB and MOB during different social interaction and in different vole species,the behaviors and neural activation of the olfactory bulbs in social interactions of mandarin voles Mi...To gain insight into the function of AOB and MOB during different social interaction and in different vole species,the behaviors and neural activation of the olfactory bulbs in social interactions of mandarin voles Microtus mandarinus and reed voles Microtus fortis were compared in the present research.Mandarin voles spent significantly more time attacking and sniffing their opponents and sniffing sawdust than reed voles.During same sex encounters,mandarin voles attacked their opponents for a significantly longer time and sniffed its opponent for shorter time compared with male-female interactions.However,no significant behavioral differences were found during encounters of two individual reed voles,regardless of gender composition of the pair.Using c-Fos as an indicator of neural activation,we observed that neural activation was significantly higher in almost all sub-regions of the main olfactory bulb(MOB)and the accessory olfactory bulb(AOB)of mandarin voles compared with reed voles.Numbers of c-Fos-ir neurons in almost all sub-regions of the AOB and the MOB during male-female interactions were also higher than those in interactions of the same sex.Anterior-posterior ratios of Fos-ir neurons in the AOBM(AOBMR)and the AOBG(AOBGR)in male-female interaction were significantly higher than those in interaction of the same sex.The AOBMR of male mandarin voles and reed voles were larger than those of females in male-female interactions.Behavioral patterns are consistent with cellular activity patterns.Consistent level of neural activation in MOB and AOB suggests important roles of both the main olfactory bulb and the accessory olfactory bulb in social interaction in two species.展开更多
Background： The first described obesity-susceptibility gene was the fat mass and obesity-associated （FTO） gene. However, knowledge about FTO＇s potential modifying effect on changes in body weight achieved through ...Background： The first described obesity-susceptibility gene was the fat mass and obesity-associated （FTO） gene. However, knowledge about FTO＇s potential modifying effect on changes in body weight achieved through a training program is still limited. We decided to study the association between the FTO A/T polymorphism （rs9939609） and obesity-related traits. Additionally, we investigated whether body mass and body composition, as well as metabolic variables observed in physically active participants, are modulated by the FTO polymorphism. Methods： A group of 201 young Polish women were recruited for the study. The genotype distribution was examined in participants measured for selected changes before and after the completion of a 12-week training program. Results： Our results confirm the association between the common FTO A/T polymorphism and increased body mass index （BMI）. Subjects with AA and AT genotypes had higher BMI during the entire study period compared with the TT genotype. Although parameters such as BMI, basal metabolism rate, tissue independence, fat mass percentage, fat mass, fat-free mass, total body water, high-density lipoprotein, and glucose changed significantly during the training program, none of the examined parameters changed significantly across the FTO genotypes （genotype × training interaction）. Conclusion： We confirm an association between the FTO A/T polymorphism and increased BMI; this polymorphism is therefore a candidate for influencing obesity and other disease-related phenotypes. Although the gene x physical activity interaction was not shown, we want to point out that promoting physical activity is an important approach to controlling the increasing obesity epidemic.2018 Published by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license. （http：//creativecommons.org/licenses/by-nc-nd/4.0/）.展开更多
Plant phenolic acids are good sources of antioxidants and sinapic acid(SA)is one of them that can be applied in protein-based food system.However,little research is available regarding interactions between almond prot...Plant phenolic acids are good sources of antioxidants and sinapic acid(SA)is one of them that can be applied in protein-based food system.However,little research is available regarding interactions between almond protein(AP)and SA.In this study,structure-affinity interaction between SA and AP,structure and antioxidant activity of proteins were investigated.Different mathematical models showed that Ka of binding SA and AP were 3.27×10^4 L/mol and 3.08×10^4 L/mol.CD(Circular dichroism)spectroscopy and FT-IR(Fourier transform infrared)spectroscopy showed that the amount of random coil andα-helix decreased whileβ-sheet increased in AP-SA complex.In combination,the interaction model of AP-SA complex was static quenching and attributed to hydrophobic interaction.Further,AP-SA complex exerted better DPPH radical scavenging ability(36.97±0.78%),ABTS+radical scavenging ability(47.26±0.45%),and higher ORAC value(2.41±0.23 M trolox/g)compared to AP.In the further,SA can be applied in protein matrix to improve film stability,gel strength and restraining fat oxidation degradation.展开更多
The full counting statistics of electron transport through two parallel quantum dots with antiparallel magnetic fluxes is investigated as a probe to detect the topological quantum-phase coherence （TQPC）, which resul...The full counting statistics of electron transport through two parallel quantum dots with antiparallel magnetic fluxes is investigated as a probe to detect the topological quantum-phase coherence （TQPC）, which results in the characteristic oscillation of the zero-frequency cumulants including the shot noise and skewness. We show explicitly the phase transition of cumulant spectrum-patterns induced by the topology change of electron path-loops while the pattern period, which depends only on the topology （or Chern number）, is robust against the variation of Coulomb interaction and interdot coupling strengths. Most importantly we report for the first time on a new type of TQPC, which is generated by the two- particle interaction and does not exist in the single-particle wave function interference. Moreover, the accurately quantized peaks of Fano-factor spectrum, which characterize the super- and sub-Poissonian shot noises, are of fundamental importance in technical applications similar to the superconducting quantum interference device.展开更多
Based on Miedema's semiempirical formation enthalpy model for binary alloys, free volume theory and ageneral solution model, a new model for prediction of activity interaction coefficient ε is proposed. The calcu...Based on Miedema's semiempirical formation enthalpy model for binary alloys, free volume theory and ageneral solution model, a new model for prediction of activity interaction coefficient ε is proposed. The calculatedresults are better in agreement with the experimental values than the two previous models. The related theories andmodels are discussed according to the degree of agreement with experimental values.展开更多
Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host.Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants u...Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host.Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions.The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera,i.e.,roots,stem and leaves of Calotropis procera(Ait.)W.T.Aiton.Plants were collected from the Lundkhwar,district Mardan.A total of 12 bacterial strains,i.e.,six from roots,three from the stem and three from the leaves were isolated.The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and easy response to the bioactive compounds present in the culture filtrate(CF)of the potent endophytic strains.The rice plants were cultivated in pots containing 30 mL of 0.8%w/v water-agar medium.The pots were placed in a growth chamber,operated at 28±0.3℃ for 14 h(day);and 25±0.3℃ for 10 h(night),at 70%relative-humidity.Among the isolated strains,R1,S1,S3,L1,R5 and R6 showed visible growth promotion in rice plants.The biochemical analysis revealed that the strains were able to produce indole acetic acid(IAA)and flavonoids in higher quantities.Moreover,the strains also produced bioactive compounds that inhibited the growth of Escherichia coli and Aspergillus flavus using the well diffusion method.From the results,it was concluded that these strains can secrete potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and,therefore,can be used as bio-fertilizer and bio-control agents.展开更多
文摘The issue of the extremely imbalanced gender ratio in preschool teachers has received widespread attention,and there are few studies on teacher-child verbal interaction behavior based on gender differences in preschool teachers.This article takes the“Little Light Bulb Is On”,a scientific exploration activity done by 5-6 years old kindergarten students as an example,and uses the improved Flanders Interaction Analysis System(iFIAS)as a tool to analyze the speech interaction behavior of male and female preschool teachers.The research results indicate that there are gender differences in teacher child language interaction between male and female teachers in terms of the atmosphere,the teaching structure,the teaching tendency,the way of raising questions,and the overall trend of interaction.
文摘Based on prior studies and a questionnaire survey,this paper is seeking to demonstrate that interactive activities will facilitate EFL learners' social strategy awareness and use,and thus enhance their linguistic development.A sample lesson plan is also presented in this paper to illustrate that social strategy awareness and use can be improved through interactive learning activities.
文摘In this paper, we conduct research on the interactive training pattern and the applications on the modern emergency rescue training activities. In emergency rescue training linkage the ecological communities, important is not gathered themselves together, and not the size of the momentum and the strength that is not the amount of emergency rescue training linkage performance, important is the ecological communities each organic elements in the function of the cohesion, lies in the relationship between the organic elements in ecological communities, the construction and the development is the specific ecological communities in action elements of emergency rescue training, joint quality lies in the emergency rescue training. These contents are involved in the emergency rescue training linkage management model of concrete. Under this basis, we integrate the condition of the Hainan Medical School to propose the corresponding suggestions that will enhance the performance.
基金the National Natural Science Foundation of China under grant No.50764006the Applied Fundamental Research Foundation of Yunnan Province under Grant No.2006E0021M are gratefully acknowledged.
文摘A novel thermodynamic model-the molecular interaction volume model （MIVM） which can be reduced to the Flory-Huggins equation of polymer solution was employed for the prediction of component activities in the ternary molten aluminosilicate slag CaO-Al2O3-SiO2 at different temperatures. The results show that the predicted values of activity of CaO, Al2O3 and SiO2 are in reasonably agreement with experimental data in some ranges of their concentrations which are about x1 〈0.25 for CaO, x2=0.05-0.55 for Al2O3 and x3=0.03-0.85 for SiO2. This further shows that MIVM requires only two binary parameters for each sub-binary system to predict activities of all components in a multicomponent solution and is the superior alternative in a molten slag.
文摘Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the foreign language.This paper will introduce some oral communicative activities that can motivate students to practice conversation skills and promote interaction.
基金supported by the National Key Research and Development Program of China(2021YFB3901205)National Institute of Natural Hazards,Ministry of Emergency Management of China(2023-JBKY-57)。
文摘The periphery of the Qinghai-Tibet Plateau is renowned for its susceptibility to landslides.However,the northwestern margin of this region,characterised by limited human activities and challenging transportation,remains insufficiently explored concerning landslide occurrence and dispersion.With the planning and construction of the Xinjiang-Tibet Railway,a comprehensive investigation into disastrous landslides in this area is essential for effective disaster preparedness and mitigation strategies.By using the human-computer interaction interpretation approach,the authors established a landslide database encompassing 13003 landslides,collectively spanning an area of 3351.24 km^(2)(36°N-40°N,73°E-78°E).The database incorporates diverse topographical and environmental parameters,including regional elevation,slope angle,slope aspect,distance to faults,distance to roads,distance to rivers,annual precipitation,and stratum.The statistical characteristics of number and area of landslides,landslide number density(LND),and landslide area percentage(LAP)are analyzed.The authors found that a predominant concentration of landslide origins within high slope angle regions,with the highest incidence observed in intervals characterised by average slopes of 20°to 30°,maximum slope angle above 80°,along with orientations towards the north(N),northeast(NE),and southwest(SW).Additionally,elevations above 4.5 km,distance to rivers below 1 km,rainfall between 20-30 mm and 30-40 mm emerge as particularly susceptible to landslide development.The study area’s geological composition primarily comprises Mesozoic and Upper Paleozoic outcrops.Both fault and human engineering activities have different degrees of influence on landslide development.Furthermore,the significance of the landslide database,the relationship between landslide distribution and environmental factors,and the geometric and morphological characteristics of landslides are discussed.The landslide H/L ratios in the study area are mainly concentrated between 0.4 and 0.64.It means the landslides mobility in the region is relatively low,and the authors speculate that landslides in this region more possibly triggered by earthquakes or located in meizoseismal area.
文摘Based on ECMWF objective analysis data, and with use of Batterworth bandpass-filtered skill and diagnostic analysis method, the interaction characteristics of low frequency wave and mean flow in midlatitudes during the winter years of 1983 / 1984 and 1986 / 1987 have been studied in this paper. The main results point out the important role of the tropical convective activity on the above mentioned interaction process.
文摘Because of the evolution of markets and technologies, prototyping concerns should be kept updated almost day by day. Moreover, user centered design moves the focus towards interaction issues. Prototyping activities matching such characteristics are already available, but they are not so diffused in the industrial domain. This is due to many reasons;an important one is that a rigorous classification of them is missing, as well as an effective helping tool for the selection of the best activities, given the design context. The research described in this paper aims at defining a new classification of prototyping activities, as well as at developing a selection algorithm to choose the best ones in an automatic way. These goals are pursued by defining a set of characteristics that allow describing accurately the prototyping activities. The resulting classification is made by five classes, based on eighteen characteristics. This classification is exploited by the first release of an algorithm for the selection of the best activities, chosen in order to satisfy design situations described thanks to a different set of eleven indices. Five experiences in the field have been used up to now as a starting point for validating the research outcomes.
基金supported by the National Basic Research Program of China (No.2010CB534912)the Doctoral Program of the Ministry of Education (No.200806570003)+1 种基金the Governor of Guizhou Province Talents Fund (No.200822)Guizhou University innovation fundsfor graduate student (No. Nongke 2012027)
文摘Objective To study the effect of fleroxacin （FLRX） on biological properties of Bloom （BLM） helicase catalytic core （BLM 642-2290 helicase） in vitro and the molecular mechanism of interaction between the two molecules. Methods DNA-binding and unwinding activities of BLM 642-1290 helicase were assayed by fluorescence polarization and gel retardation assay under conditions that the helicase was subjected to different concentrations of FLRX. Effect of FLRX on helicase ATPase activity was analyzed by phosphorus-free assay based on a colorimetric estimation of ATP hydrolysis-produced inorganic phosphate. Molecular mechanism of interaction between the two molecules was assayed by ultraviolet and fluorescence spectra. Results The DNA unwinding and ATPase activities of BLM 642-1290 helicase were inhibited whereas the DNA-binding activity was promoted in vitro. A BLM-FLRX complex was formed through one binding site, electrostatic and hydrophobic interaction force. Moreover, the intrinsic fluorescence of the helicase was quenched by FLRX as a result of non-radioactive energy transfer. The biological activity of helicase was affected by FLRX, which may be through an allosteric mechanism and stabilization of enzyme conformation in low helicase activity state, disruption of the coupling of ATP hydrolysis to unwinding, and blocking helicase translocation on DNA strands. Conclusion FLRX may affect the biological activities and conformation of BLM 642-1290 helicase, and DNA helicase may be used as a promising drug target for some diseases.
文摘The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After reaction, tuffaceous slate became light colored and soft, and its mass density reduced. The amount of gold extracted from tuffaceous slate ranges widely, from 0 027 to 0 234 μg/g. Chlorine solution may activate appreciable amount of gold, and the gold migratory rate is high enough, from 50 70% to 92 30%, which reveals that sulphur and chlorine work together in solutions to accelerate gold activation and migration, and to realize gold mineralization in favorable places.
文摘Gold catalysts have been reported as highly effective catalysts in various oxidation reactions.However,for chemoselective hydrogenation reactions,gold‐based catalysts normally show much lowercatalytic activity than platinum group metals,even though their selectivities are excellent.Here,wereport that the chemoselective hydrogenation activity of 3‐nitrostyrene to 3‐vinylaniline overAu/TiO_(2)can be enhanced up to 3.3 times through the hydrogen reduction strategy.It is revealedthat strong metal‐support interaction,between gold nanoparticles(NPs)and TiO_(2)support,is introducedthrough hydrogen reduction,resulting in partial dispersion of reduced TiOx on the Au surface.The partially covered Au not only increases the perimeter of the interface between the gold NPs andthe support,but also benefits H_(2)activation.Reaction kinetic analysis and H_(2)‐D2 exchange reactionshow that H_(2)activation is the critical step in the hydrogenation of 3‐nitrostyrene to 3‐vinylaniline.Density functional theory calculations verify that hydrogen dissociation and hydrogen transfer arefavored at the interface of gold NPs and TiO_(2)over the hydrogen‐reduced Au/TiO_(2).This study providesinsights for fabricating highly active gold‐based catalysts for chemoselective hydrogenationreactions.
基金the center of the current manuscript was performed as a component of the Operation Brain Trauma Therapy consortium,which is supported by U.S.Army grants W81XWH-10-1-0623 and WH81XWH-14-2-0018Microscopy was performed at the VCU Department of Anatomy and Neurobiology Microscopy Facility,supported,in part,with funding from NIH-NINDS Center core grant 5P30NS047463
文摘Axonal injury is a pathological hallmark of both head injury and inflammatory-mediated neurological disorders,including multiple sclerosis（Schirmer et al.,2013）.Such axonal disruptions and/or disconnections typically result in proximal axonal segments that remain in continuity with the neuronal somawhile losing contact with their distal targets.
基金supported by the Ministry of Science and Technology(2016YFA0400904 and 2018YFA0208702)the National Natural Foundation of China(No.21973085,No.21833007,No.21790350,No.21673212,No.21521001,and No.21473165)+1 种基金the Fundamental Research Funds for the Central Universities(No.WK2340000074)Anhui Initiative in Quantum Information Technologies(No.AHY090200).
文摘From the organization of animal flocks to the emergence of swarming behaviors in bacterial suspension,populations of motile organisms at all scales display coherent collective motion.Recent studies showed that the anisotropic interaction between active particles plays a key role in the phase behaviors.Here we investigate the collective behaviors of based-active Janus particles that experience an anisotropic interaction of which the orientation is opposite to the direction of active force by using Langevin dynamics simulations in two dimensional space.Interestingly,the system shows emergence of collective swarming states upon increasing the total area fraction of particles,which is not observed in systems without anisotropic interaction or activity.The threshold for emergence of swarming states decreases as particle activity or interaction strength increases.We have also performed basic kinetic analysis to reproduce the essential features of the simulation results.Our results demonstrate that anisotropic interactions at the individual level are sufficient to set homogeneous active particles into stable directed motion.
基金supported by National Natural Science Foundation of China(No.30670273No.30200026)Ministry of Education Key Project of Peoples Republic of China(20060718)
文摘To gain insight into the function of AOB and MOB during different social interaction and in different vole species,the behaviors and neural activation of the olfactory bulbs in social interactions of mandarin voles Microtus mandarinus and reed voles Microtus fortis were compared in the present research.Mandarin voles spent significantly more time attacking and sniffing their opponents and sniffing sawdust than reed voles.During same sex encounters,mandarin voles attacked their opponents for a significantly longer time and sniffed its opponent for shorter time compared with male-female interactions.However,no significant behavioral differences were found during encounters of two individual reed voles,regardless of gender composition of the pair.Using c-Fos as an indicator of neural activation,we observed that neural activation was significantly higher in almost all sub-regions of the main olfactory bulb(MOB)and the accessory olfactory bulb(AOB)of mandarin voles compared with reed voles.Numbers of c-Fos-ir neurons in almost all sub-regions of the AOB and the MOB during male-female interactions were also higher than those in interactions of the same sex.Anterior-posterior ratios of Fos-ir neurons in the AOBM(AOBMR)and the AOBG(AOBGR)in male-female interaction were significantly higher than those in interaction of the same sex.The AOBMR of male mandarin voles and reed voles were larger than those of females in male-female interactions.Behavioral patterns are consistent with cellular activity patterns.Consistent level of neural activation in MOB and AOB suggests important roles of both the main olfactory bulb and the accessory olfactory bulb in social interaction in two species.
基金supported by National Science Centre of Poland(No.2012/07/B/NZ7/01155)
文摘Background： The first described obesity-susceptibility gene was the fat mass and obesity-associated （FTO） gene. However, knowledge about FTO＇s potential modifying effect on changes in body weight achieved through a training program is still limited. We decided to study the association between the FTO A/T polymorphism （rs9939609） and obesity-related traits. Additionally, we investigated whether body mass and body composition, as well as metabolic variables observed in physically active participants, are modulated by the FTO polymorphism. Methods： A group of 201 young Polish women were recruited for the study. The genotype distribution was examined in participants measured for selected changes before and after the completion of a 12-week training program. Results： Our results confirm the association between the common FTO A/T polymorphism and increased body mass index （BMI）. Subjects with AA and AT genotypes had higher BMI during the entire study period compared with the TT genotype. Although parameters such as BMI, basal metabolism rate, tissue independence, fat mass percentage, fat mass, fat-free mass, total body water, high-density lipoprotein, and glucose changed significantly during the training program, none of the examined parameters changed significantly across the FTO genotypes （genotype × training interaction）. Conclusion： We confirm an association between the FTO A/T polymorphism and increased BMI; this polymorphism is therefore a candidate for influencing obesity and other disease-related phenotypes. Although the gene x physical activity interaction was not shown, we want to point out that promoting physical activity is an important approach to controlling the increasing obesity epidemic.2018 Published by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license. （http：//creativecommons.org/licenses/by-nc-nd/4.0/）.
基金supported by the National Key Research and Development Program of China (2016YFD0401401)The Technological innovation project of Hubei Province (2017ABA142)+2 种基金The Science and Technology Plan Project of Tibet Autonomous Region (XZ201901NA04)The Science and Technology Plan Project of Hunan Science (2017NK2212)The Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2016-OCRI)
文摘Plant phenolic acids are good sources of antioxidants and sinapic acid(SA)is one of them that can be applied in protein-based food system.However,little research is available regarding interactions between almond protein(AP)and SA.In this study,structure-affinity interaction between SA and AP,structure and antioxidant activity of proteins were investigated.Different mathematical models showed that Ka of binding SA and AP were 3.27×10^4 L/mol and 3.08×10^4 L/mol.CD(Circular dichroism)spectroscopy and FT-IR(Fourier transform infrared)spectroscopy showed that the amount of random coil andα-helix decreased whileβ-sheet increased in AP-SA complex.In combination,the interaction model of AP-SA complex was static quenching and attributed to hydrophobic interaction.Further,AP-SA complex exerted better DPPH radical scavenging ability(36.97±0.78%),ABTS+radical scavenging ability(47.26±0.45%),and higher ORAC value(2.41±0.23 M trolox/g)compared to AP.In the further,SA can be applied in protein matrix to improve film stability,gel strength and restraining fat oxidation degradation.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11075099.11204203,and 11275118)
文摘The full counting statistics of electron transport through two parallel quantum dots with antiparallel magnetic fluxes is investigated as a probe to detect the topological quantum-phase coherence （TQPC）, which results in the characteristic oscillation of the zero-frequency cumulants including the shot noise and skewness. We show explicitly the phase transition of cumulant spectrum-patterns induced by the topology change of electron path-loops while the pattern period, which depends only on the topology （or Chern number）, is robust against the variation of Coulomb interaction and interdot coupling strengths. Most importantly we report for the first time on a new type of TQPC, which is generated by the two- particle interaction and does not exist in the single-particle wave function interference. Moreover, the accurately quantized peaks of Fano-factor spectrum, which characterize the super- and sub-Poissonian shot noises, are of fundamental importance in technical applications similar to the superconducting quantum interference device.
文摘Based on Miedema's semiempirical formation enthalpy model for binary alloys, free volume theory and ageneral solution model, a new model for prediction of activity interaction coefficient ε is proposed. The calculatedresults are better in agreement with the experimental values than the two previous models. The related theories andmodels are discussed according to the degree of agreement with experimental values.
基金This research was supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2017R1D1A1B04035601).
文摘Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host.Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions.The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera,i.e.,roots,stem and leaves of Calotropis procera(Ait.)W.T.Aiton.Plants were collected from the Lundkhwar,district Mardan.A total of 12 bacterial strains,i.e.,six from roots,three from the stem and three from the leaves were isolated.The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and easy response to the bioactive compounds present in the culture filtrate(CF)of the potent endophytic strains.The rice plants were cultivated in pots containing 30 mL of 0.8%w/v water-agar medium.The pots were placed in a growth chamber,operated at 28±0.3℃ for 14 h(day);and 25±0.3℃ for 10 h(night),at 70%relative-humidity.Among the isolated strains,R1,S1,S3,L1,R5 and R6 showed visible growth promotion in rice plants.The biochemical analysis revealed that the strains were able to produce indole acetic acid(IAA)and flavonoids in higher quantities.Moreover,the strains also produced bioactive compounds that inhibited the growth of Escherichia coli and Aspergillus flavus using the well diffusion method.From the results,it was concluded that these strains can secrete potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and,therefore,can be used as bio-fertilizer and bio-control agents.
