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Evaluation of Abuse Drugs and Clinical Laboratory Tests Variations in Whole Blood & Urine Samples of Abusers 被引量:1
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作者 Majid Rezaei Basiri Mojgan Behshid +9 位作者 Alireza Najafi Saleh Alilou Haniyeh Mohebbi-Kamali: Fatemeh Hosseindoust Marziyeh Mokhtari Valiyollah Watani Ladan Aminzadeh Fatemeh Seyed Nejad Ashraf Razavi Ligha Saadat 《Journal of Pharmacy and Pharmacology》 2018年第1期69-76,共8页
In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples ... In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples of addicts in clinics of welfare organization, during detoxification treatment or maintenance treatment were screened for abuse drugs presence. The all of samples were tested through as a view of clinical laboratory methods. Age ranges of female patients were 35 ~ 15 and age range of males patients were 45 ~ 15. All patients filled questionnaire and satisfy forms too. First, all fresh urine and blood samples were examined to confirm presence drugs abuses, depend on their addiction and treatment, so all samples were confirmed by two tests. Then they were examined to other clinical laboratory tests. All data were analyzed by t-test and were Anova one way and two ways of Anova Turkey, and p 〈 0.05 was considered significant. The p-value of this study was p = 0.0001. The results of this study were showed that 4% of abusers had mild increase in hematocrite level and 2% of narcotic drugs abusers had mild lower level of blood sugars than normal range and 4% of participants had increase liver enzymes such ALT (alanine transferase), AST (aspartat transferase), ALP (alkaline phosphatease) and 1% of them had renal failure. Although blood level BUN (blood urea nitrogen) and creatinin were examined to evaluation of their renal failure .The results in Tabriz/Iran undrevision of welfare organization clinics were approximately showed that positive results of addiction are in each of urine and blood samples. Because some of abusers directly consumed full long time agonist or partial agonists' drugs such as methadone and buprenorphine for their maintenance therapy in clinics. Also doing test on blood samples has high importance in distinguishing and confirmation of drugs abuse in samples. Also in this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis, also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse, and serum levels of some other parameters showed all abusers patients situation such as liver and renal dysfimction through clinical laboratory tests. 展开更多
关键词 Abusers urine and whole blood samples strip test thin-layer chromatography abuse drugs clinical laboratory tests.
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Direct analysis method of^(14)C radioactivity concentration in urine samples
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作者 Xiao Ge Linlin Yan +5 位作者 Qiang Xiong Fei Chen Jing Liang Zhen Zhang Hailan Yang Dingxin Long 《Radiation Medicine and Protection》 2022年第1期26-30,共5页
Objective:To establish a rapid analysis method for the activity concentration of carbon-14(14C)in urine,in order to estimate the internal dose of 14C exposure,and to protect the health of occupational population.Metho... Objective:To establish a rapid analysis method for the activity concentration of carbon-14(14C)in urine,in order to estimate the internal dose of 14C exposure,and to protect the health of occupational population.Methods:Liquid scintillation counting(LSC)combined with the function of transformed spectral index of the external standard spectrum(tSIE)was used to measure the quenching level and counting rate,and the spiked urine samples with different shades of color were measured by LSC.After establishing the efficiency-quenching curve,the quenching correction and activity concentration analysis of the actual samples were carried out.Results:By LSC and the data fitting,the relationship between efficiency and quenching index could be represented using the equation y=0.0013x-0.0177(R^(2)=0.978).Three actual spiked samples were tested to verify this method,with recoveries of 97%,102%,and 89%,respectively.14C activity concentration of 4 actual urine samples were 0.12,0.11,0.10 and 0.08 Bq/mL,respectively,while the corresponding extended relative uncertainties were 0.0652,0.0929,0.0893 and 0.1043,respectively.Conclusion:The direct analysis method of 14C activity concentration in urine samples was established using LSC.The recovery of 14C activity concentration in urine samples showed that the proposed method had relatively high accuracy.By studying on the source of uncertainty,the uncertainty of the analysis results mainly came from the statistical error of LSC,and the uncertainty component of counting efficiency. 展开更多
关键词 ^(14)C urine sample Direct analysis Radioactivity concentration
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Distinguishing of Abuse Drugs in Urine and Blood Samples of Abusers in Iran 被引量:1
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作者 Rezaei-Basiri Majid Behshid Mojgan +6 位作者 Ghazi-Khansari Mahmoud Tabatabaei Seyed Mahmoud Moradi Danesh Alilu Saleh Jabbarzadeh Aahad Mokhtari Marziyeh Bahari Afrouz 《Journal of Pharmacy and Pharmacology》 2015年第8期385-390,共6页
In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples re... In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples referred who addicts in clinics of Welfare Organization, during of detoxification treatment or maintenance treatment were screened for abuse drugs presence. Age ranges of female patients were 35 ± 15 and age range of males patients were 42 ± 18. All patients filled questionnaire and satisfy forms too. All data were analyzed by t-test and were Anowa one way, and P 〈 0.05 was considered significant. The P value of this study was P = 0.000. In this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis and TLC (thin-layer chromatography) is appropriate confirmation method to drug abuse distinguishing. Also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse. 展开更多
关键词 Abusers urine and blood samples Strip test Thin-layer chromatography abuse drugs.
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Determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction and capillary electrophoresis with PDA detection
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作者 Liang Meng Shuhai Ye +1 位作者 Yilin Wu Linda You 《Forensic Sciences Research》 CSCD 2022年第2期265-271,共7页
A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array... A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array detection.The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent(chloroform)dissolved in water-miscible organic dispersive solvent(isopropyl alcohol).The organic phase,which extracted eight drugs of abuse from the prepared urine solution,was separated by centrifugation.The sedimented phase was transferred into a small volume CE auto-sampler vial with 10μL of 1%HCl methanol solution and evaporated to dryness.The residue was reconstituted in lidocaine hydrochloride(internal standard)aqueous solution and introduced by electrokinetic injection into CE.Under the optimum conditions,acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient(r)of 0.9982–0.9994 for spiked urine samples.The limit of detection(LOD)(S/N=3)was estimated to be 1.0 ng/mL.A recovery of 75.7%–90.6%was obtained for spiked samples.The mean relative error(MRE)was within±7.0%and the relative standard deviation(RSD)was less than 6.9%.The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples. 展开更多
关键词 Forensic sciences forensic toxicology dispersive liquid-liquid microextraction capillary electrophoresis drugs of abuse urine sample
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An AIEgens and exonuclease Ⅲ aided quadratic amplification assay for detecting and cellular imaging of telomerase activity 被引量:5
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作者 Xuehong Min Lei Xia +5 位作者 Yuan Zhuang Xudong Wang Jie Du Xiaojin Zhang Xiaoding Lou Fan Xia 《Science Bulletin》 SCIE EI CAS CSCD 2017年第14期997-1003,共7页
Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified ... Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe (TPE-Py-DNA) as a fluorescence reporter and exonuclease llI (Exo lIl) as a signal amplifier. With the aid of telomerase, repeat units (TrAGGG)n are extended from the end of template substrate oligonucleotides (TS primer) that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 :N2. This strategy exhibits good performance for detecting clinical urine samples (distinguishing 15 cancer patients' samples from 8 healthy ones) and checking intracellular telomerase activity (differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug), which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer. 展开更多
关键词 Quadratic amplification AIEgens Telomerase urine samples Exonuclease Ill Cell imaging
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