Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically li...Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.展开更多
An airborne pushbroom hyperspectrai imager （APHI） with wide field （42° field of view） is presented. It is composed of two 22° field of view （FOV） imagers and can provide 1304 pixels in spatial dimensio...An airborne pushbroom hyperspectrai imager （APHI） with wide field （42° field of view） is presented. It is composed of two 22° field of view （FOV） imagers and can provide 1304 pixels in spatial dimension, 124 bands in spectral dimension in one frame. APHI has a bandwidth ranging from 400 to 900 nm. The spectral resolution is 5 nm and the spatial resolution is 0.6 m at 1000-m height. The implementation of this system is helpful to overcome the restriction of FOV in pushbroom hyperspectral imaging in a more feasible way. The electronic and optical designs axe also introduced in detail.展开更多
The high-altitude detection of astronomical radiation(HADAR)experiment is a new Cherenkov observation technique with a wide field of view(FoV),aimed at observing the prompt emissions ofγ-ray bursts(GRBs).The bottlene...The high-altitude detection of astronomical radiation(HADAR)experiment is a new Cherenkov observation technique with a wide field of view(FoV),aimed at observing the prompt emissions ofγ-ray bursts(GRBs).The bottleneck for this type of experiment can be found in determining how to reject the high rate of nightsky background(NSB)noise from random stars.In this work,we propose a novel method for rejecting noise,which considers the spatial properties of GRBs and the temporal characteristics of Cherenkov radiation.In space coordinates,the map between the celestial sphere and the fired photomultiplier tubes(PMTs)on the telescope's camera can be expressed as f(δ(i,j))=δ'(i',j'),which means that a limited number of PMTs is selected from one direction.On the temporal scale,a 20-ns time window was selected based on the knowledge of Cherenkov radiation.This allowed integration of the NSB for a short time interval.Consequently,the angular resolution and effective area at 100 GeV in the HADAR experiment were obtained as 0.2°and 10^(4)m^(2),respectively.This method can be applied to all wide-FoV experiments.展开更多
This paper presents a method for structured scene modeling using micro stereo vision system with large field of view. The proposed algorithm includes edge detection with Canny detector, line fitting with principle axi...This paper presents a method for structured scene modeling using micro stereo vision system with large field of view. The proposed algorithm includes edge detection with Canny detector, line fitting with principle axis based approach, finding corresponding lines using feature based matching method, and 3D line depth computation.展开更多
Optical imaging has served as a primary method to collect information about biosystems across scales—from functionalities of tissues to morphological structures of cells and even at biomolecular levels.However,to ade...Optical imaging has served as a primary method to collect information about biosystems across scales—from functionalities of tissues to morphological structures of cells and even at biomolecular levels.However,to adequately characterize a complex biosystem,an imaging system with a number of resolvable points,referred to as a space-bandwidth product(SBP),in excess of one billion is typically needed.Since a gigapixel-scale far exceeds the capacity of current optical imagers,compromises must be made to obtain either a low spatial resolution or a narrow field-of-view(FOV).The problem originates from constituent refractive optics—the larger the aperture,the more challenging the correction of lens aberrations.Therefore,it is impractical for a conventional optical imaging system to achieve an SBP over hundreds of millions.To address this unmet need,a variety of high-SBP imagers have emerged over the past decade,enabling an unprecedented resolution and FOV beyond the limit of conventional optics.We provide a comprehensive survey of high-SBP imaging techniques,exploring their underlying principles and applications in bioimaging.展开更多
Histopathology relies upon the staining and sectioning of biological tissues,which can be laborious and may cause artifacts and distort tissues.We develop label-free volumetric imaging of thick-tissue slides,exploitin...Histopathology relies upon the staining and sectioning of biological tissues,which can be laborious and may cause artifacts and distort tissues.We develop label-free volumetric imaging of thick-tissue slides,exploiting refractive index distributions as intrinsic imaging contrast.The present method systematically exploits label-free quantitative phase imaging techniques,volumetric reconstruction of intrinsic refractive index distributions in tissues,and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image distortion.We demonstrated label-free volumetric imaging of thick tissues with the field of view of 2 mm×1.75 mm×0.2 mm with a spatial resolution of 170 nm×170 nm×1400 nm.The number of optical modes,calculated as the reconstructed volume divided by the size of the point spread function,was∼20 giga voxels.We have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method.展开更多
基金partially supported by the Gordon and Betty Moore Foundation Grant No.5722
文摘Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.
基金This work was supported by the National "863" High Technology Project of China (No. 2001AA131019).
文摘An airborne pushbroom hyperspectrai imager （APHI） with wide field （42° field of view） is presented. It is composed of two 22° field of view （FOV） imagers and can provide 1304 pixels in spatial dimension, 124 bands in spectral dimension in one frame. APHI has a bandwidth ranging from 400 to 900 nm. The spectral resolution is 5 nm and the spatial resolution is 0.6 m at 1000-m height. The implementation of this system is helpful to overcome the restriction of FOV in pushbroom hyperspectral imaging in a more feasible way. The electronic and optical designs axe also introduced in detail.
基金supported by the Key R&D Program of Sichuan Province (Nos. 2019ZYZF0001 and 2020YFSY0016)the National Natural Science Foundation of China (Nos. 11873005,12047575, 11705103, 11635011, U1831208, U1632104, 11875264U2031110)
文摘The high-altitude detection of astronomical radiation(HADAR)experiment is a new Cherenkov observation technique with a wide field of view(FoV),aimed at observing the prompt emissions ofγ-ray bursts(GRBs).The bottleneck for this type of experiment can be found in determining how to reject the high rate of nightsky background(NSB)noise from random stars.In this work,we propose a novel method for rejecting noise,which considers the spatial properties of GRBs and the temporal characteristics of Cherenkov radiation.In space coordinates,the map between the celestial sphere and the fired photomultiplier tubes(PMTs)on the telescope's camera can be expressed as f(δ(i,j))=δ'(i',j'),which means that a limited number of PMTs is selected from one direction.On the temporal scale,a 20-ns time window was selected based on the knowledge of Cherenkov radiation.This allowed integration of the NSB for a short time interval.Consequently,the angular resolution and effective area at 100 GeV in the HADAR experiment were obtained as 0.2°and 10^(4)m^(2),respectively.This method can be applied to all wide-FoV experiments.
文摘This paper presents a method for structured scene modeling using micro stereo vision system with large field of view. The proposed algorithm includes edge detection with Canny detector, line fitting with principle axis based approach, finding corresponding lines using feature based matching method, and 3D line depth computation.
基金supported partially by the National Institutes of Health(R01EY029397,R35GM128761)the National Science Foundation(1652150)+1 种基金support from the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2019R1A6A3A03031505)support from the National Science Foundation(1846784)。
文摘Optical imaging has served as a primary method to collect information about biosystems across scales—from functionalities of tissues to morphological structures of cells and even at biomolecular levels.However,to adequately characterize a complex biosystem,an imaging system with a number of resolvable points,referred to as a space-bandwidth product(SBP),in excess of one billion is typically needed.Since a gigapixel-scale far exceeds the capacity of current optical imagers,compromises must be made to obtain either a low spatial resolution or a narrow field-of-view(FOV).The problem originates from constituent refractive optics—the larger the aperture,the more challenging the correction of lens aberrations.Therefore,it is impractical for a conventional optical imaging system to achieve an SBP over hundreds of millions.To address this unmet need,a variety of high-SBP imagers have emerged over the past decade,enabling an unprecedented resolution and FOV beyond the limit of conventional optics.We provide a comprehensive survey of high-SBP imaging techniques,exploring their underlying principles and applications in bioimaging.
基金H.H.,R.H.H.,S.-M.H.,and Y.P.conceived the initial idea.H.H.developed the optical system and analysis methods.H.H.and Y.W.K.performed the experiments and analyzed the data.M.L.and S.S.provided the analysis methods and analyzed the data.All authors wrote and revised the manuscript.This work was supported by KAIST,Up Program,BK21+program,Tomocube,and National Research Foundation of Korea(2017M3C1A3013923,2015R1A3A2066550,and 2018K000396).Professor Park and Mr.Moosung Lee have financial interests in Tomocube Inc.,a company that commercializes optical diffraction tomography and quantitative phase imaging instruments and is one of the sponsors of the work.
文摘Histopathology relies upon the staining and sectioning of biological tissues,which can be laborious and may cause artifacts and distort tissues.We develop label-free volumetric imaging of thick-tissue slides,exploiting refractive index distributions as intrinsic imaging contrast.The present method systematically exploits label-free quantitative phase imaging techniques,volumetric reconstruction of intrinsic refractive index distributions in tissues,and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image distortion.We demonstrated label-free volumetric imaging of thick tissues with the field of view of 2 mm×1.75 mm×0.2 mm with a spatial resolution of 170 nm×170 nm×1400 nm.The number of optical modes,calculated as the reconstructed volume divided by the size of the point spread function,was∼20 giga voxels.We have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method.
