Objective: To investigate the role of 99mTc-TRODAT-1 SPECT in diagnosis and assessing severity of idiopathic Parkinson's disease (PD). Methods: Thirty-eight patients with primary, tentative diagnosis of PD and eig...Objective: To investigate the role of 99mTc-TRODAT-1 SPECT in diagnosis and assessing severity of idiopathic Parkinson's disease (PD). Methods: Thirty-eight patients with primary, tentative diagnosis of PD and eighteen age-matched normal controls were studied with 99mTc-TRODAT-1 SPECT imaging. The regions of interests (ROIs) were drawn manually on cerebellum (CB), occipital cortex (OC) and three transverse plane slice-views of striatums, the semiquantitative BG (background)/[(OC+CB)/2] were then calculated. Results: A lower uptake of 99mTc-TRODAT-Ⅰ in striatums were displayed in thirty-six out of thirty-eight PD patients by visual inspection, compared to controls. In twenty-four PD cases with HYS (Hoehn and Yahr scale) stage I, a greater loss of DAT uptake was found in striatum and its subregions contralateral striatum to the affected limbs than in the same regions of the controls, although the striatal uptake was bilaterally reduced. Using Spearman correlation analysis showed that the reduction of the uptake ratios significantly correlated with the UPDRS in striatum and all its subregions in the PD group (P＜0.05), a similar change was also found in the putamen by using the rating scale of Hoehn and Yahr (P＜0.05).However, analysis of variance (ANOVA) did not show any relationship between the decreasing uptake of 99mTc-TRODAT-1 and increasing severity of PD patients, although the specific uptake of 99mTc-TRODAT-Ⅰ was continuously decreased in the striatum by visual inspection with the progress of PD from HYS stage Ⅰ to Ⅲ. Conclusion: 99mTc-TRODAT- 1 SPECT imaging may serve as a useful method for improving the correct diagnosis of PD. In assessing the role of99mTc-TRODAT- 1 SPECT in disease severity of PD, UPDRS can offer a comprehensive index, although the Hoehn and Yahr assessment may be available in part.展开更多
Dopamine transporter (DAT) mediates the regulation of dopaminergic function. Two agents of TRODAT-1 and FP-CIT were observed in evaluating DAT change of Parkinson's disease (PD). The relationship between them was ...Dopamine transporter (DAT) mediates the regulation of dopaminergic function. Two agents of TRODAT-1 and FP-CIT were observed in evaluating DAT change of Parkinson's disease (PD). The relationship between them was also evaluated. The results suggested that 99mTc-TRODAT-1 SPECT and 131I-FP-CIT SPECT may serve as sensitive and objective in vivo markers to reflect the severity of PD. The 99mTc-TRODAT-1 image is more accurate and clearer compared with 131I-FP-CIT.展开更多
To prevent and treat Parkinson's disease in its early stages,it is essential to be able to detect the degree of early dopaminergic neuron degeneration.Dopamine transporters(DAT) in the striatum regulate synaptic d...To prevent and treat Parkinson's disease in its early stages,it is essential to be able to detect the degree of early dopaminergic neuron degeneration.Dopamine transporters(DAT) in the striatum regulate synaptic dopamine levels,and striatal ^(99m)Tc-TRODAT-1 single-photon emission computed tomography(-SPECT) imaging is a marker for presynaptic neuronal degeneration.However,the association between the degree of dopaminergic degeneration and in vivo ^(99m)Tc-TRODAT-1 SPECT imaging is unknown.Therefore,this study investigated the association between the degree of 6-hydroxydopamine(6-OHDA)-induced dopaminergic degeneration and DAT imaging using ^(99m)Tc-TRODAT-1 SPECT in rats.Different degrees of nigrostriatal dopamine depletion were generated by injecting different doses of 6-OHDA(2,4,and 8 μg) into the right medial forebrain bundle.The degree of nigrostriatal dopaminergic neuron degeneration was assessed by rotational behavior and immunohistochemical staining.The results showed that striatal ^(99m)Tc-TRODAT-1 binding was significantly diminished both in the ipsilateral and the contralateral sides in the 4 and 8 μg 6-OHDA groups,and that DAT ^(99m)Tc-TRODAT-1 binding in the ipsilateral striatum showed a high correlation to apomorphine-induced rotations at 8 weeks post-lesion(r = –0.887,P < 0.01).There were significant correlations between DAT ^(99m)Tc-TRODAT-1 binding in the ipsilateral striatum and the amount of tyrosine hydroxylase immunoreactive neurons in the ipsilateral substantia nigra in the 2,4,and 8 μg 6-OHDA groups at 8 weeks post-lesion(r = 0.899,P < 0.01).These findings indicate that striatal DAT imaging using ^(99m)Tc-TRODAT-1 is a useful technique for evaluating the severity of dopaminergic degeneration.展开更多
In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with ...In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia.Pigmented guinea pigs aged 3 weeks were derided into form deprivation myopia (FDM) group (n=6) and normal control group (n=6).The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4^(th) week after injection of ^(99m)Tc-TRODAT-1.The retinas were clearly resolved in the images.The ratio of ^(99m)Tc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98),and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04).The results showed that ^(99m)Tc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina,and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes.Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia.展开更多
Objective:Evaluate the application values of 99mTc-2 β[N, N′-bis(2-mercaptoethy1)ethylenediamino]methyl, 3β-(4-chlorophenyl)tropane(TRODAT-1) dopamine transporter (DAT) SPECT imaging in children autism, and offer t...Objective:Evaluate the application values of 99mTc-2 β[N, N′-bis(2-mercaptoethy1)ethylenediamino]methyl, 3β-(4-chlorophenyl)tropane(TRODAT-1) dopamine transporter (DAT) SPECT imaging in children autism, and offer the academic foundation to etiology, mechanism and clinical therapy of autism. Methods:Ten autistic children and ten healthy controls were examined with 99mTc-TRODAT-1 DAT SPECT imaging. Striatal specific uptake of 99mTc-TRODAT-1 was calculated with region of interest analysis according to the ratios between striatum and cerebellum[(STR-BKG)/BKG]. Results:There was no difference in semiquantitative dopamine transporter between bilateral striatum in autistic children (P=0.562) and in normal controls (P=0.573); dopamine transporter in brain of patients with autism increased more significantly than that in normal controls (P=0.017).Conclusion:Dopaminergic nervous system is dysfunction in human brain with children autism, and DAT 99mTc-TRODAT-1 SPECT imaging on human brain will help the imaging diagnosis of children autism.展开更多
文摘Objective: To investigate the role of 99mTc-TRODAT-1 SPECT in diagnosis and assessing severity of idiopathic Parkinson's disease (PD). Methods: Thirty-eight patients with primary, tentative diagnosis of PD and eighteen age-matched normal controls were studied with 99mTc-TRODAT-1 SPECT imaging. The regions of interests (ROIs) were drawn manually on cerebellum (CB), occipital cortex (OC) and three transverse plane slice-views of striatums, the semiquantitative BG (background)/[(OC+CB)/2] were then calculated. Results: A lower uptake of 99mTc-TRODAT-Ⅰ in striatums were displayed in thirty-six out of thirty-eight PD patients by visual inspection, compared to controls. In twenty-four PD cases with HYS (Hoehn and Yahr scale) stage I, a greater loss of DAT uptake was found in striatum and its subregions contralateral striatum to the affected limbs than in the same regions of the controls, although the striatal uptake was bilaterally reduced. Using Spearman correlation analysis showed that the reduction of the uptake ratios significantly correlated with the UPDRS in striatum and all its subregions in the PD group (P＜0.05), a similar change was also found in the putamen by using the rating scale of Hoehn and Yahr (P＜0.05).However, analysis of variance (ANOVA) did not show any relationship between the decreasing uptake of 99mTc-TRODAT-1 and increasing severity of PD patients, although the specific uptake of 99mTc-TRODAT-Ⅰ was continuously decreased in the striatum by visual inspection with the progress of PD from HYS stage Ⅰ to Ⅲ. Conclusion: 99mTc-TRODAT- 1 SPECT imaging may serve as a useful method for improving the correct diagnosis of PD. In assessing the role of99mTc-TRODAT- 1 SPECT in disease severity of PD, UPDRS can offer a comprehensive index, although the Hoehn and Yahr assessment may be available in part.
基金National "Tenth Five" Scientific Technique Research Plan Project (2001BA702B02)
文摘Dopamine transporter (DAT) mediates the regulation of dopaminergic function. Two agents of TRODAT-1 and FP-CIT were observed in evaluating DAT change of Parkinson's disease (PD). The relationship between them was also evaluated. The results suggested that 99mTc-TRODAT-1 SPECT and 131I-FP-CIT SPECT may serve as sensitive and objective in vivo markers to reflect the severity of PD. The 99mTc-TRODAT-1 image is more accurate and clearer compared with 131I-FP-CIT.
基金supported by the National Natural Science Foundation of China,No.81571250
文摘To prevent and treat Parkinson's disease in its early stages,it is essential to be able to detect the degree of early dopaminergic neuron degeneration.Dopamine transporters(DAT) in the striatum regulate synaptic dopamine levels,and striatal ^(99m)Tc-TRODAT-1 single-photon emission computed tomography(-SPECT) imaging is a marker for presynaptic neuronal degeneration.However,the association between the degree of dopaminergic degeneration and in vivo ^(99m)Tc-TRODAT-1 SPECT imaging is unknown.Therefore,this study investigated the association between the degree of 6-hydroxydopamine(6-OHDA)-induced dopaminergic degeneration and DAT imaging using ^(99m)Tc-TRODAT-1 SPECT in rats.Different degrees of nigrostriatal dopamine depletion were generated by injecting different doses of 6-OHDA(2,4,and 8 μg) into the right medial forebrain bundle.The degree of nigrostriatal dopaminergic neuron degeneration was assessed by rotational behavior and immunohistochemical staining.The results showed that striatal ^(99m)Tc-TRODAT-1 binding was significantly diminished both in the ipsilateral and the contralateral sides in the 4 and 8 μg 6-OHDA groups,and that DAT ^(99m)Tc-TRODAT-1 binding in the ipsilateral striatum showed a high correlation to apomorphine-induced rotations at 8 weeks post-lesion(r = –0.887,P < 0.01).There were significant correlations between DAT ^(99m)Tc-TRODAT-1 binding in the ipsilateral striatum and the amount of tyrosine hydroxylase immunoreactive neurons in the ipsilateral substantia nigra in the 2,4,and 8 μg 6-OHDA groups at 8 weeks post-lesion(r = 0.899,P < 0.01).These findings indicate that striatal DAT imaging using ^(99m)Tc-TRODAT-1 is a useful technique for evaluating the severity of dopaminergic degeneration.
文摘In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia.Pigmented guinea pigs aged 3 weeks were derided into form deprivation myopia (FDM) group (n=6) and normal control group (n=6).The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4^(th) week after injection of ^(99m)Tc-TRODAT-1.The retinas were clearly resolved in the images.The ratio of ^(99m)Tc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98),and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04).The results showed that ^(99m)Tc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina,and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes.Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia.
文摘Objective:Evaluate the application values of 99mTc-2 β[N, N′-bis(2-mercaptoethy1)ethylenediamino]methyl, 3β-(4-chlorophenyl)tropane(TRODAT-1) dopamine transporter (DAT) SPECT imaging in children autism, and offer the academic foundation to etiology, mechanism and clinical therapy of autism. Methods:Ten autistic children and ten healthy controls were examined with 99mTc-TRODAT-1 DAT SPECT imaging. Striatal specific uptake of 99mTc-TRODAT-1 was calculated with region of interest analysis according to the ratios between striatum and cerebellum[(STR-BKG)/BKG]. Results:There was no difference in semiquantitative dopamine transporter between bilateral striatum in autistic children (P=0.562) and in normal controls (P=0.573); dopamine transporter in brain of patients with autism increased more significantly than that in normal controls (P=0.017).Conclusion:Dopaminergic nervous system is dysfunction in human brain with children autism, and DAT 99mTc-TRODAT-1 SPECT imaging on human brain will help the imaging diagnosis of children autism.
