Objective Intracellular formation of Lewy body （LB） is one of the hallmarks of Parkinson’s disease.The main component of LB is aggregatedα-synuclein,present in the substantia nigra where iron accumulation also occ...Objective Intracellular formation of Lewy body （LB） is one of the hallmarks of Parkinson’s disease.The main component of LB is aggregatedα-synuclein,present in the substantia nigra where iron accumulation also occurs.The present study was aimed to study the relationship between iron andα-synuclein aggregation.Methods SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h.MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detectα-synuclein aggregation.Results With the increase of iron concentration,the cell viability decreased significantly.At the concentrations of 5 mmol/L and 10 mmol/L,iron inducedα-synuclein aggregation more severely than at the concentration of 1 mmol/L.Besides,48-h treatment-induced aggregation was more severe than that induced by 24-h treatment,at the corresponding iron concentrations.Conclusion Ferric iron can induceα-synuclein aggregation,which is toxic to the cells,in a dose-and time-dependent way.展开更多
基金supported by the grants from the National Basic Research Development Program of China(No.2006CB500704)the National Natural Science Foundation of China(No.30930036,30870858)the Natural Science Fund for Distinguished Young Scholars of Shandong Province,China(No.JQ200807)
文摘Objective Intracellular formation of Lewy body （LB） is one of the hallmarks of Parkinson’s disease.The main component of LB is aggregatedα-synuclein,present in the substantia nigra where iron accumulation also occurs.The present study was aimed to study the relationship between iron andα-synuclein aggregation.Methods SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h.MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detectα-synuclein aggregation.Results With the increase of iron concentration,the cell viability decreased significantly.At the concentrations of 5 mmol/L and 10 mmol/L,iron inducedα-synuclein aggregation more severely than at the concentration of 1 mmol/L.Besides,48-h treatment-induced aggregation was more severe than that induced by 24-h treatment,at the corresponding iron concentrations.Conclusion Ferric iron can induceα-synuclein aggregation,which is toxic to the cells,in a dose-and time-dependent way.
