AIM:To investigate the safety of β-L-D4A on DNA polymeraseα. METHODS:Ion exchange chromatography was used to separate DNA polymeraseαfrom crude extract of human Hela cells.Detailed kinetic parameters were determine...AIM:To investigate the safety of β-L-D4A on DNA polymeraseα. METHODS:Ion exchange chromatography was used to separate DNA polymeraseαfrom crude extract of human Hela cells.Detailed kinetic parameters were determined for β-L-D4A against DNA polymeraseα. RESULTS:DNA polymeraseαwas purified with 4% yield and 31000 units/rag specific activity.The Michaelis constant (Km=3.22μmol/L),50% inhibition concentration (IC50=178.49μmol/L) and inhibition constant (Ki=126μmol/L) of β-L-D4A were determined by kinetic analysis. CONCLUSION:β-L-D4A is a more safe nucleoside for hepatitis B virus (HBV) infection with a lower host toxicity.展开更多
In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was ...In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.展开更多
基金National Natural Science Foundation of China,No.30330680
文摘AIM:To investigate the safety of β-L-D4A on DNA polymeraseα. METHODS:Ion exchange chromatography was used to separate DNA polymeraseαfrom crude extract of human Hela cells.Detailed kinetic parameters were determined for β-L-D4A against DNA polymeraseα. RESULTS:DNA polymeraseαwas purified with 4% yield and 31000 units/rag specific activity.The Michaelis constant (Km=3.22μmol/L),50% inhibition concentration (IC50=178.49μmol/L) and inhibition constant (Ki=126μmol/L) of β-L-D4A were determined by kinetic analysis. CONCLUSION:β-L-D4A is a more safe nucleoside for hepatitis B virus (HBV) infection with a lower host toxicity.
文摘In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.
