Our previous work proved that high adsorption capacity and uptake rate of lysozyme were achieved on alginate(Alg)-grafted re sin with an ionic capacity(IC) of 240 mmol·L^(-1)(Alg-FF-240).Moreover,the salt-toleran...Our previous work proved that high adsorption capacity and uptake rate of lysozyme were achieved on alginate(Alg)-grafted re sin with an ionic capacity(IC) of 240 mmol·L^(-1)(Alg-FF-240).Moreover,the salt-tolerant feature of Alg-FF-230 was improved by using sequential alginate grafting and sulfonation strategy.Inspired by the enhanced adsorption performance of lysozyme,we have herein proposed to investigate the static and dynamic adsorption behaviors of γ-globulin on a series of Alg-grafted resins with different grafting densities and sulfonation degrees.The adsorption ca pacity of γ-globulin decreased with increa sing alginate-grafting density(IC) from 160 to 230 mmol·L^(-1) at 0 mmol·L^(-1) NaCl because of the steric hindrance caused by the alginate-grafting layer.Effects of ionic strength(IS) indicated that the adsorption capacities of the resins with the IC value of 230-370 mmol·L^(-1) were much higher than CM Sepharose FF at 50-100 mmol·L^(-1) NaCl,and the uptake rate of Alg-FF-230 was about twice as much as that of CM Sepharose FF.This work demonstrated the important effects of alginate-grafting layer and IS in γ-globulin adsorption behavior,which would be helpful in the design of Alggrafted resins and the selection of proper IS condition for protein purification.展开更多
γ-globulin is a globulin which possesses immune special characteristic. Steven et al. investigated the binding of Gd<sup>3+</sup> to non-immune rabbit immunoglobulin G and its fragments by measurements of...γ-globulin is a globulin which possesses immune special characteristic. Steven et al. investigated the binding of Gd<sup>3+</sup> to non-immune rabbit immunoglobulin G and its fragments by measurements of the enhancement of the solvent-water展开更多
Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6-Diaminohe...Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6-Diaminohexane (DAH) as space arm and phenylalanine (Phe) as ligand were immobilized onto nylon membrane through the activation of s-triazine trichloride (CyCl3). The affinity membrane obtained can be used to adsorb γ-globulin specifically. The optimum conditions of preparing affinity membrane and adsorbing bovine γ-globulin were studied.展开更多
目的:探讨丙种球蛋白无反应及敏感川崎病患儿血小板(PLT)、干扰素-γ(IFN-γ)、白介素-6(IL-6)表达水平的差异及临床意义。方法:选取2019年5月至2022年6月厦门大学附属中山医院收治的99例川崎病患儿,根据丙种球蛋白治疗反应性分为无反应...目的:探讨丙种球蛋白无反应及敏感川崎病患儿血小板(PLT)、干扰素-γ(IFN-γ)、白介素-6(IL-6)表达水平的差异及临床意义。方法:选取2019年5月至2022年6月厦门大学附属中山医院收治的99例川崎病患儿,根据丙种球蛋白治疗反应性分为无反应组24例和敏感组75例,比较两组基线水平及12 h、36 h PLT、IFN-γ、IL-6表达水平。采用Logistic回归分析川崎病丙种球蛋白无反应的相关影响因素,采用受试者工作特征曲线(ROC)分析PLT、IFN-γ、IL-6及联合预测川崎病丙种球蛋白无反应的价值,采用DeLong检验不同方案的预测价值,采用临床决策曲线分析法(DCA)分析PLT+IFN-γ+IL-6方案预测川崎病丙种球蛋白反应性的临床效用。结果:无反应组12 h及36 h PLT、IFN-γ、IL-6表达水平与基线水平比较,差异无统计学意义(P>0.05);敏感组12 h及36 h PLT、IFN-γ、IL-6表达水平均低于基线水平(P<0.05);无反应组基线水平及12 h、36 h PLT、IFN-γ、IL-6表达水平高于敏感组(P<0.05);基线PLT、IFN-γ、IL-6均为川崎病丙种球蛋白无反应的独立相关影响因素(P<0.05);PLT、IFN-γ、IL-6及联合的ROC曲线下面积(AUC)依次为0.818、0.767、0.712、0.915,PLT+IFN-γ+IL-6的AUC高于IFN-γ、IL-6(P<0.05),与PLT比较,差异无统计学意义(P>0.05);采用PLT+IFN-γ+IL-6方案预测川崎病丙种球蛋白无反应具有临床正向的净获益。结论:川崎病丙种球蛋白无反应和敏感患儿PLT、IFN-γ、IL-6表达水平存在显著差异,与患儿治疗反应性有关,联合检测PLT、IFN-γ、IL-6可预测丙种球蛋白治疗反应。展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21878222 and 21621004)。
文摘Our previous work proved that high adsorption capacity and uptake rate of lysozyme were achieved on alginate(Alg)-grafted re sin with an ionic capacity(IC) of 240 mmol·L^(-1)(Alg-FF-240).Moreover,the salt-tolerant feature of Alg-FF-230 was improved by using sequential alginate grafting and sulfonation strategy.Inspired by the enhanced adsorption performance of lysozyme,we have herein proposed to investigate the static and dynamic adsorption behaviors of γ-globulin on a series of Alg-grafted resins with different grafting densities and sulfonation degrees.The adsorption ca pacity of γ-globulin decreased with increa sing alginate-grafting density(IC) from 160 to 230 mmol·L^(-1) at 0 mmol·L^(-1) NaCl because of the steric hindrance caused by the alginate-grafting layer.Effects of ionic strength(IS) indicated that the adsorption capacities of the resins with the IC value of 230-370 mmol·L^(-1) were much higher than CM Sepharose FF at 50-100 mmol·L^(-1) NaCl,and the uptake rate of Alg-FF-230 was about twice as much as that of CM Sepharose FF.This work demonstrated the important effects of alginate-grafting layer and IS in γ-globulin adsorption behavior,which would be helpful in the design of Alggrafted resins and the selection of proper IS condition for protein purification.
文摘γ-globulin is a globulin which possesses immune special characteristic. Steven et al. investigated the binding of Gd<sup>3+</sup> to non-immune rabbit immunoglobulin G and its fragments by measurements of the enhancement of the solvent-water
基金Project (No. 29675026) supported by the National Natural Science Foundation of China.
文摘Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6-Diaminohexane (DAH) as space arm and phenylalanine (Phe) as ligand were immobilized onto nylon membrane through the activation of s-triazine trichloride (CyCl3). The affinity membrane obtained can be used to adsorb γ-globulin specifically. The optimum conditions of preparing affinity membrane and adsorbing bovine γ-globulin were studied.
文摘目的:探讨丙种球蛋白无反应及敏感川崎病患儿血小板(PLT)、干扰素-γ(IFN-γ)、白介素-6(IL-6)表达水平的差异及临床意义。方法:选取2019年5月至2022年6月厦门大学附属中山医院收治的99例川崎病患儿,根据丙种球蛋白治疗反应性分为无反应组24例和敏感组75例,比较两组基线水平及12 h、36 h PLT、IFN-γ、IL-6表达水平。采用Logistic回归分析川崎病丙种球蛋白无反应的相关影响因素,采用受试者工作特征曲线(ROC)分析PLT、IFN-γ、IL-6及联合预测川崎病丙种球蛋白无反应的价值,采用DeLong检验不同方案的预测价值,采用临床决策曲线分析法(DCA)分析PLT+IFN-γ+IL-6方案预测川崎病丙种球蛋白反应性的临床效用。结果:无反应组12 h及36 h PLT、IFN-γ、IL-6表达水平与基线水平比较,差异无统计学意义(P>0.05);敏感组12 h及36 h PLT、IFN-γ、IL-6表达水平均低于基线水平(P<0.05);无反应组基线水平及12 h、36 h PLT、IFN-γ、IL-6表达水平高于敏感组(P<0.05);基线PLT、IFN-γ、IL-6均为川崎病丙种球蛋白无反应的独立相关影响因素(P<0.05);PLT、IFN-γ、IL-6及联合的ROC曲线下面积(AUC)依次为0.818、0.767、0.712、0.915,PLT+IFN-γ+IL-6的AUC高于IFN-γ、IL-6(P<0.05),与PLT比较,差异无统计学意义(P>0.05);采用PLT+IFN-γ+IL-6方案预测川崎病丙种球蛋白无反应具有临床正向的净获益。结论:川崎病丙种球蛋白无反应和敏感患儿PLT、IFN-γ、IL-6表达水平存在显著差异,与患儿治疗反应性有关,联合检测PLT、IFN-γ、IL-6可预测丙种球蛋白治疗反应。
