This study was designed to elucidate the relationship between root chemical signals and the quality of rice. Various ricegenotypes were used. Zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), 1-aminocylopropane ...This study was designed to elucidate the relationship between root chemical signals and the quality of rice. Various ricegenotypes were used. Zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), 1-aminocylopropane -1-carboxylic acid(ACC), and organic acids in roots during grain filling and the appearance quality, cooking/eating quality were investigated.The correlations among them were analyzed. The results showed that Z + ZR concentrations in the roots at mid- and late-grain-filling stages were significantly and positively correlated with the gel consistency and alkali spreading value (r =0.72* - 0.90**), whereas negatively correlated with the amylose content (r = -0.68* - -0.78**). ABA concentrations in rootsat mid- grain-filling stage were significantly and negatively correlated with the gel consistency and alkali spreading value(r = -0.90** - -0.91**), and positively correlated with the amylose content (r = 0.87**). ACC concentrations in root exudatesat mid-grain-filling stage were very significantly correlated with the percentage of chalky grains and chalkiness (r = 0.97**- 0.98**), and those at late-grain-filling stage were significantly correlated with chalkiness and chalky size (r = 0.69* -0.96**). The more the malic acid and succinic acid exuded from roots for a cultivar, the greater the breakdown values andthe smaller the setback values in the starch profile, and the results were reversed for a cultivar with more tartaric acid andcitric acid exuded from roots during the grain-filling period. The cultivar with more lactic acid in exudates had smaller gelconsistency and alkali spreading values, but had greater amylose content. When roots were treated with exogenous ZR,ABA, and ACC during grain filling, effects of the chemicals on the rice quality were consistent with the relationships ofthe endogenous hormones (Z + ZR, ABA, and ACC) with the quality indexes. Using rape cake as organic fertilizer canincrease the concentrations of malic acid and succinic acids exuded from roots as well as the breakdown value in starchprofile and reduce the setback value. The results suggest that root chemical signals play important roles in the formationof rice quality, and rice quality could be improved through regulating the signals.展开更多
目的:分析NFKBIA基因多态性、饮食习惯及其交互作用与胃癌易感性的关系。方法:采用1∶1配对病例对照研究设计,收集2013年4月-2017年6月福建仙游县医院587例胃癌新发病例和按性别、年龄匹配的587例健康人群为研究对象,采用自制调查表收...目的:分析NFKBIA基因多态性、饮食习惯及其交互作用与胃癌易感性的关系。方法:采用1∶1配对病例对照研究设计,收集2013年4月-2017年6月福建仙游县医院587例胃癌新发病例和按性别、年龄匹配的587例健康人群为研究对象,采用自制调查表收集一般情况、饮食习惯等资料,利用Sequenom Mass ARRAY SNP方法检测外周血NFKBIA基因rs696位点的基因型。应用条件logistic回归模型分析饮食习惯、基因型对胃癌发病风险的影响,利用叉生分析结合logistic回归模型和Andersson等编制的Excel表进行基因与环境的交互作用分析。结果:快速饮食、不规律进餐、高盐饮食和经常摄入腌菜是胃癌发生的危险因素,OR(95%CI)分别为1.53(1.20,1.95)、1.55(1.16,2.08)、1.51(1.17,1.94)、2.78(2.01,3.85);NFKBIA基因rs696位点携带AG基因型、AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险。此外,rs696位点AA基因型与高盐饮食、经常摄入腌菜存在联合作用,rs696位点显性模型(AG+AA)与快速饮食、高盐饮食、经常摄入腌菜存在联合作用,均增加胃贲门癌发病风险。结论:不良饮食习惯是胃癌发生的危险因素,NFKBIA基因rs696位点AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险,且与饮食习惯存在联合作用。展开更多
Eating disorder is a group of physiological and psychological disorders affecting approximately 1%of the female population worldwide.Although the genetic epidemiology of eating disorder is becoming increasingly clear ...Eating disorder is a group of physiological and psychological disorders affecting approximately 1%of the female population worldwide.Although the genetic epidemiology of eating disorder is becoming increasingly clear with accumulated studies,the underlying molecular mechanisms are still unclear.Recently,integration of various high-throughput data expanded the range of candidate genes and started to generate hypotheses for understanding potential pathogenesis in complex diseases.This article presents EDdb(Eating Disorder database),the first evidence-based gene resource for eating disorder.Fifty-nine experimentally validated genes from the literature in relation to eating disorder were collected as the core dataset.Another four datasets with 2824 candidate genes across 601 genome regions were expanded based on the core dataset using different criteria(e.g.,protein-protein interactions,shared cytobands,and related complex diseases).Based on human protein-protein interaction data,we reconstructed a potential molecular sub-network related to eating disorder.Furthermore,with an integrative pathway enrichment analysis of genes in EDdb,we identified an extended adipocytokine signaling pathway in eating disorder.Three genes in EDdb(ADIPO(adiponectin),TNF(tumor necrosis factor)and NR3C1(nuclear receptor subfamily 3,group C,member 1))link the KEGG(Kyoto Encyclopedia of Genes and Genomes)"adipocytokine signaling pathway"with the BioCarta"visceral fat deposits and the metabolic syndrome"pathway to form a joint pathway.In total,the joint pathway contains 43genes,among which 39 genes are related to eating disorder.As the first comprehensive gene resource for eating disorder,EDdb(http://eddb.cbi.pku.edu.cn)enables the exploration of gene-disease relationships and cross-talk mechanisms between related disorders.Through pathway statistical studies,we revealed that abnormal body weight caused by eating disorder and obesity may both be related to dysregulation of the novel joint pathway of adipocytokine signaling.In addition,this joint pathway may be the common pathway for body weight regulation in complex human diseases related to unhealthy lifestyle.展开更多
基金supported by the National Natural Science Foundation of China(30370828)the Natural Science Foundation of Jiangsu Province,China(BK200341).
文摘This study was designed to elucidate the relationship between root chemical signals and the quality of rice. Various ricegenotypes were used. Zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), 1-aminocylopropane -1-carboxylic acid(ACC), and organic acids in roots during grain filling and the appearance quality, cooking/eating quality were investigated.The correlations among them were analyzed. The results showed that Z + ZR concentrations in the roots at mid- and late-grain-filling stages were significantly and positively correlated with the gel consistency and alkali spreading value (r =0.72* - 0.90**), whereas negatively correlated with the amylose content (r = -0.68* - -0.78**). ABA concentrations in rootsat mid- grain-filling stage were significantly and negatively correlated with the gel consistency and alkali spreading value(r = -0.90** - -0.91**), and positively correlated with the amylose content (r = 0.87**). ACC concentrations in root exudatesat mid-grain-filling stage were very significantly correlated with the percentage of chalky grains and chalkiness (r = 0.97**- 0.98**), and those at late-grain-filling stage were significantly correlated with chalkiness and chalky size (r = 0.69* -0.96**). The more the malic acid and succinic acid exuded from roots for a cultivar, the greater the breakdown values andthe smaller the setback values in the starch profile, and the results were reversed for a cultivar with more tartaric acid andcitric acid exuded from roots during the grain-filling period. The cultivar with more lactic acid in exudates had smaller gelconsistency and alkali spreading values, but had greater amylose content. When roots were treated with exogenous ZR,ABA, and ACC during grain filling, effects of the chemicals on the rice quality were consistent with the relationships ofthe endogenous hormones (Z + ZR, ABA, and ACC) with the quality indexes. Using rape cake as organic fertilizer canincrease the concentrations of malic acid and succinic acids exuded from roots as well as the breakdown value in starchprofile and reduce the setback value. The results suggest that root chemical signals play important roles in the formationof rice quality, and rice quality could be improved through regulating the signals.
文摘目的:分析NFKBIA基因多态性、饮食习惯及其交互作用与胃癌易感性的关系。方法:采用1∶1配对病例对照研究设计,收集2013年4月-2017年6月福建仙游县医院587例胃癌新发病例和按性别、年龄匹配的587例健康人群为研究对象,采用自制调查表收集一般情况、饮食习惯等资料,利用Sequenom Mass ARRAY SNP方法检测外周血NFKBIA基因rs696位点的基因型。应用条件logistic回归模型分析饮食习惯、基因型对胃癌发病风险的影响,利用叉生分析结合logistic回归模型和Andersson等编制的Excel表进行基因与环境的交互作用分析。结果:快速饮食、不规律进餐、高盐饮食和经常摄入腌菜是胃癌发生的危险因素,OR(95%CI)分别为1.53(1.20,1.95)、1.55(1.16,2.08)、1.51(1.17,1.94)、2.78(2.01,3.85);NFKBIA基因rs696位点携带AG基因型、AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险。此外,rs696位点AA基因型与高盐饮食、经常摄入腌菜存在联合作用,rs696位点显性模型(AG+AA)与快速饮食、高盐饮食、经常摄入腌菜存在联合作用,均增加胃贲门癌发病风险。结论:不良饮食习惯是胃癌发生的危险因素,NFKBIA基因rs696位点AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险,且与饮食习惯存在联合作用。
基金supported by the National High Technology Research and Development Program of China(2008BAI64B01)the National Natural Science Foundation of China(31171270)
文摘Eating disorder is a group of physiological and psychological disorders affecting approximately 1%of the female population worldwide.Although the genetic epidemiology of eating disorder is becoming increasingly clear with accumulated studies,the underlying molecular mechanisms are still unclear.Recently,integration of various high-throughput data expanded the range of candidate genes and started to generate hypotheses for understanding potential pathogenesis in complex diseases.This article presents EDdb(Eating Disorder database),the first evidence-based gene resource for eating disorder.Fifty-nine experimentally validated genes from the literature in relation to eating disorder were collected as the core dataset.Another four datasets with 2824 candidate genes across 601 genome regions were expanded based on the core dataset using different criteria(e.g.,protein-protein interactions,shared cytobands,and related complex diseases).Based on human protein-protein interaction data,we reconstructed a potential molecular sub-network related to eating disorder.Furthermore,with an integrative pathway enrichment analysis of genes in EDdb,we identified an extended adipocytokine signaling pathway in eating disorder.Three genes in EDdb(ADIPO(adiponectin),TNF(tumor necrosis factor)and NR3C1(nuclear receptor subfamily 3,group C,member 1))link the KEGG(Kyoto Encyclopedia of Genes and Genomes)"adipocytokine signaling pathway"with the BioCarta"visceral fat deposits and the metabolic syndrome"pathway to form a joint pathway.In total,the joint pathway contains 43genes,among which 39 genes are related to eating disorder.As the first comprehensive gene resource for eating disorder,EDdb(http://eddb.cbi.pku.edu.cn)enables the exploration of gene-disease relationships and cross-talk mechanisms between related disorders.Through pathway statistical studies,we revealed that abnormal body weight caused by eating disorder and obesity may both be related to dysregulation of the novel joint pathway of adipocytokine signaling.In addition,this joint pathway may be the common pathway for body weight regulation in complex human diseases related to unhealthy lifestyle.
