Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved b...Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68~2.75 mg·L -1 and 0.07~0.54 mg·L -1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.展开更多
文摘Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68~2.75 mg·L -1 and 0.07~0.54 mg·L -1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.