Suspension cell cultures of Maytenus hookeri Loos. (Celastraceae) in SH media were established from the calli induced from the leaves and young steins of M. hookeri on MS media with the supplement of 2 mg/L 2,4-D and ...Suspension cell cultures of Maytenus hookeri Loos. (Celastraceae) in SH media were established from the calli induced from the leaves and young steins of M. hookeri on MS media with the supplement of 2 mg/L 2,4-D and 0.1 mg/L KIN (kinetin). Ethyl acetate extract of the cultures showed inhibitory activities against Penicillium avellaneum UC-4376 which was sensitive to maytansinoids. Exhaustive isolation of natural products from a large scale of suspension cell cultures did not yield maytansine instead of affording nine compounds including one novel triterpenoid, named 2, 3-diacetoxyl maytenusone (1), and eight known ones including squalene (2), beta-sitosterol (3), 2', 3', 4-triacetyl-sitoindoside I (4), salaspermic acid (5), maytenonic acid (6), 2alpha-hydroxy-maytenonic acid (7), 6, 11,12-trihydroxy-8, 11, 13-abietrien-7-one (8) and 11, 12-dihydroxy-8, 11, 13-abietatrien-7-one (9) elucidated on the basis of 1D and 2D NMR data. The H-1-NMR and C-13-NMR assignments were made for 1, 5, 6 and 7, while the C-13-NMR assignments for 5 and 6 were revised. The chemical results suggested that the suspension cell cultures of M. hookeri did not produce maytansinoids under the reported experiment conditions.展开更多
The Chinese traditional aesthetic culture not only pay attention to the overall sense of He Xie （Harmony, 和谐）, and pay attention to a kind of artistic conception as Zi Wei （滋味）, Qi Yun （氣韵）, and YiJing （...The Chinese traditional aesthetic culture not only pay attention to the overall sense of He Xie （Harmony, 和谐）, and pay attention to a kind of artistic conception as Zi Wei （滋味）, Qi Yun （氣韵）, and YiJing （意境） as well. Mr. JI Xian-lin had tried to start from Zi Wei （滋味）, a new comprehension to open a new understanding on Chinese aesthetics. Hence, we embark from the Chinese aesthetic culture and its basic spirit, the humanistic connotation of Zi Wei （滋味）, which sounds with the basis, put forward it into a Music-Dance Spirit （乐舞精神） in Chinese art through this unique view, has made a new thinking to the Chinese traditional aesthetic artistic category system.展开更多
Cloud water samples, LWC （Liquid Water Content） and meteorological data were collected at the Clingmans Dome, Tennessee, high-elevation site in Great Smoky Mountains National Park during the warm season from 1994 th...Cloud water samples, LWC （Liquid Water Content） and meteorological data were collected at the Clingmans Dome, Tennessee, high-elevation site in Great Smoky Mountains National Park during the warm season from 1994 through 2011. This paper presents results from 2000 through the conclusion of the study in 2011. Samples were analyzed for SO42＂, NO3, NH4＋ and H＋. These measurements were supplemented by measurements of ambient air and precipitation concentrations to estimate dry and wet deposition. Cloud water concentrations, LWC, cloud frequency, various meteorological measurements and information on nearby forest canopy were used to model cloud water deposition to gauge trends in deposition. Total deposition was calculated as the sum of cloud, dry and wet deposition estimates. Concentrations and deposition fluxes declined over the study period. The decreases in cloud water SO42＂ and NO3 concentrations were 40 percent and 26 percent, respectively. Three-year mean 5042 and NO3 deposition rates decreased by 71 percent and 70 percent, respectively. Trends in concentrations and depositions were comparable with trends in SO2 and NOx emissions from Tennessee Valley Authority power plants and aggregated emission reductions from electric generating units in adjacent states. Back trajectories were simulated with the HYSPLIT model and aggregated over cloud sampling periods from 2000 through 2007 and 2009 through 2011. Trajectories during periods with high H＋ concentrations traveled over local EGU （Electric Generating Unit） emission sources in Tennessee and Kentucky to the Ohio River Valley, Alabama and Georgia with the conclusion that these source regions contributed to acidic cloud water deposition at Clingmans Dome. This work was supported by U.S. Environmental Protection Agency and the Tennessee Valley Authority with infrastructure support provided by the National Park Service.展开更多
文摘Suspension cell cultures of Maytenus hookeri Loos. (Celastraceae) in SH media were established from the calli induced from the leaves and young steins of M. hookeri on MS media with the supplement of 2 mg/L 2,4-D and 0.1 mg/L KIN (kinetin). Ethyl acetate extract of the cultures showed inhibitory activities against Penicillium avellaneum UC-4376 which was sensitive to maytansinoids. Exhaustive isolation of natural products from a large scale of suspension cell cultures did not yield maytansine instead of affording nine compounds including one novel triterpenoid, named 2, 3-diacetoxyl maytenusone (1), and eight known ones including squalene (2), beta-sitosterol (3), 2', 3', 4-triacetyl-sitoindoside I (4), salaspermic acid (5), maytenonic acid (6), 2alpha-hydroxy-maytenonic acid (7), 6, 11,12-trihydroxy-8, 11, 13-abietrien-7-one (8) and 11, 12-dihydroxy-8, 11, 13-abietatrien-7-one (9) elucidated on the basis of 1D and 2D NMR data. The H-1-NMR and C-13-NMR assignments were made for 1, 5, 6 and 7, while the C-13-NMR assignments for 5 and 6 were revised. The chemical results suggested that the suspension cell cultures of M. hookeri did not produce maytansinoids under the reported experiment conditions.
文摘The Chinese traditional aesthetic culture not only pay attention to the overall sense of He Xie （Harmony, 和谐）, and pay attention to a kind of artistic conception as Zi Wei （滋味）, Qi Yun （氣韵）, and YiJing （意境） as well. Mr. JI Xian-lin had tried to start from Zi Wei （滋味）, a new comprehension to open a new understanding on Chinese aesthetics. Hence, we embark from the Chinese aesthetic culture and its basic spirit, the humanistic connotation of Zi Wei （滋味）, which sounds with the basis, put forward it into a Music-Dance Spirit （乐舞精神） in Chinese art through this unique view, has made a new thinking to the Chinese traditional aesthetic artistic category system.
文摘Cloud water samples, LWC （Liquid Water Content） and meteorological data were collected at the Clingmans Dome, Tennessee, high-elevation site in Great Smoky Mountains National Park during the warm season from 1994 through 2011. This paper presents results from 2000 through the conclusion of the study in 2011. Samples were analyzed for SO42＂, NO3, NH4＋ and H＋. These measurements were supplemented by measurements of ambient air and precipitation concentrations to estimate dry and wet deposition. Cloud water concentrations, LWC, cloud frequency, various meteorological measurements and information on nearby forest canopy were used to model cloud water deposition to gauge trends in deposition. Total deposition was calculated as the sum of cloud, dry and wet deposition estimates. Concentrations and deposition fluxes declined over the study period. The decreases in cloud water SO42＂ and NO3 concentrations were 40 percent and 26 percent, respectively. Three-year mean 5042 and NO3 deposition rates decreased by 71 percent and 70 percent, respectively. Trends in concentrations and depositions were comparable with trends in SO2 and NOx emissions from Tennessee Valley Authority power plants and aggregated emission reductions from electric generating units in adjacent states. Back trajectories were simulated with the HYSPLIT model and aggregated over cloud sampling periods from 2000 through 2007 and 2009 through 2011. Trajectories during periods with high H＋ concentrations traveled over local EGU （Electric Generating Unit） emission sources in Tennessee and Kentucky to the Ohio River Valley, Alabama and Georgia with the conclusion that these source regions contributed to acidic cloud water deposition at Clingmans Dome. This work was supported by U.S. Environmental Protection Agency and the Tennessee Valley Authority with infrastructure support provided by the National Park Service.
