AIM:To investigate whether the secretion of phospha-tidylcholine(PC)in intestinal mucus occurs by apical secretion or via basolateral excretion and to determine its subsequent passage across the tight junctions to the...AIM:To investigate whether the secretion of phospha-tidylcholine(PC)in intestinal mucus occurs by apical secretion or via basolateral excretion and to determine its subsequent passage across the tight junctions to the apical mucus.METHODS:We addressed this question using the po-larized intestinally differentiated tumor cell line CaCo-2 grown on filters to confluence in Transwell culture chambers.The released PC and sphingomyelin(Sph)from apical and basolateral media were analyzed by mass spectrometry.RESULTS:The secreted PC species were identical in both compartments indicating the same intracellular origin of PC.However,PC secretion into the basolateral compart-ment was more effective,and the PC:Sph ratio in the ba-solateral compartment was signif icantly higher than that in the apical compartment(8.18 ± 1.84 vs 4.31 ± 1.22,P = 0.01).Both pathways were temperature sensitive and were unaltered in the presence of cyclosporine.CONCLUSION:The data demonstrate the PC secre-tion capacity of CaCo-2 cells and indicate two sepa-rated apical and basolateral release mechanisms.展开更多
Fifteen inorganic salts were evaluated as possible sterilants against the Red Palm Weevil （RPW） Rhvachophorus ferrugineus, by applying the pupal dipping method in a screening program. Results showed that all tested ...Fifteen inorganic salts were evaluated as possible sterilants against the Red Palm Weevil （RPW） Rhvachophorus ferrugineus, by applying the pupal dipping method in a screening program. Results showed that all tested salts adversely affected the adtflt emergence and reduced the egg laying capacity and egg hatchability. Among the tested salts Ca（OH）2. LiC1, CuCl2, ZnSO4, KBr and KI gave various 97%-100% percent sterility at the tested non toxic concentration under different pupal dipping periods. Percentage of sterility increased with the increase of the concentration and the dipping period. All other salts induced degrees oi sterility ranging from 63% to 96%.展开更多
基金Supported by A Grant From the Dietmar Hopp Foundation (Stremmel WR)the Post-Doc programme of the Medical Faculty of the University of Heidelberg (Gotthardt D and Braun A)
文摘AIM:To investigate whether the secretion of phospha-tidylcholine(PC)in intestinal mucus occurs by apical secretion or via basolateral excretion and to determine its subsequent passage across the tight junctions to the apical mucus.METHODS:We addressed this question using the po-larized intestinally differentiated tumor cell line CaCo-2 grown on filters to confluence in Transwell culture chambers.The released PC and sphingomyelin(Sph)from apical and basolateral media were analyzed by mass spectrometry.RESULTS:The secreted PC species were identical in both compartments indicating the same intracellular origin of PC.However,PC secretion into the basolateral compart-ment was more effective,and the PC:Sph ratio in the ba-solateral compartment was signif icantly higher than that in the apical compartment(8.18 ± 1.84 vs 4.31 ± 1.22,P = 0.01).Both pathways were temperature sensitive and were unaltered in the presence of cyclosporine.CONCLUSION:The data demonstrate the PC secre-tion capacity of CaCo-2 cells and indicate two sepa-rated apical and basolateral release mechanisms.
文摘Fifteen inorganic salts were evaluated as possible sterilants against the Red Palm Weevil （RPW） Rhvachophorus ferrugineus, by applying the pupal dipping method in a screening program. Results showed that all tested salts adversely affected the adtflt emergence and reduced the egg laying capacity and egg hatchability. Among the tested salts Ca（OH）2. LiC1, CuCl2, ZnSO4, KBr and KI gave various 97%-100% percent sterility at the tested non toxic concentration under different pupal dipping periods. Percentage of sterility increased with the increase of the concentration and the dipping period. All other salts induced degrees oi sterility ranging from 63% to 96%.
